Title : A magnetic nanoprobe technology for detecting molecular interactions in live cells - Won_2005_Science_309_121 |
Author(s) : Won J , Kim M , Yi YW , Kim YH , Jung N , Kim TK |
Ref : Science , 309 :121 , 2005 |
Abstract :
Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins. |
PubMedSearch : Won_2005_Science_309_121 |
PubMedID: 15994554 |
Won J, Kim M, Yi YW, Kim YH, Jung N, Kim TK (2005)
A magnetic nanoprobe technology for detecting molecular interactions in live cells
Science
309 :121
Won J, Kim M, Yi YW, Kim YH, Jung N, Kim TK (2005)
Science
309 :121