Title : Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides - Wu_2014_Biosens.Bioelectron_53_26 |
Author(s) : Wu C , Liu X , Li Y , Du X , Wang X , Xu P |
Ref : Biosensors & Bioelectronics , 53 :26 , 2014 |
Abstract :
For triglycerides biosensor design, protein immobilization is necessary to create the interface between the enzyme and the electrode. In this study, a glassy carbon electrode (GCE) was modified with lipase-nanoporous gold (NPG) biocomposite (denoted as lipase/NPG/GCE). Due to highly conductive, porous, and biocompatible three-dimensional structure, NPG is suitable for enzyme immobilization. In cyclic voltammetry experiments, the lipase/NPG/GCE bioelectrode displayed surface-confined reaction in a phosphate buffer solution. Linear responses were obtained for tributyrin concentrations ranging from 50 to 250 mg dl(-1) and olive oil concentrations ranging from 10 to 200 mg dl(-1). The value of apparent Michaelis-Menten constant for tributyrin was 10.67 mg dl(-1) and the detection limit was 2.68 mg dl(-1). Further, the lipase/NPG/GCE bioelectrode had strong anti-interference ability against urea, glucose, cholesterol, and uric acid as well as a long shelf-life. For the detection of triglycerides in human serum, the values given by the lipase/NPG/GCE bioelectrode were in good agreement with those of an automatic biochemical analyzer. These properties along with a long self-life make the lipase/NPG/GCE bioelectrode an excellent choice for the construction of triglycerides biosensor. |
PubMedSearch : Wu_2014_Biosens.Bioelectron_53_26 |
PubMedID: 24121205 |
Wu C, Liu X, Li Y, Du X, Wang X, Xu P (2014)
Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides
Biosensors & Bioelectronics
53 :26
Wu C, Liu X, Li Y, Du X, Wang X, Xu P (2014)
Biosensors & Bioelectronics
53 :26