| Title : Rapid Mining of Novel alpha-Glucosidase and Lipase Inhibitors from Streptomyces sp. HO1518 Using UPLC-QTOF-MS\/MS - Xu_2022_Mar.Drugs_20_ |
| Author(s) : Xu J , Liu Z , Feng Z , Ren Y , Liu H , Wang Y |
| Ref : Mar Drugs , 20 : , 2022 |
| Abstract : A rapid and sensitive method using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) was applied for the analysis of the metabolic profile of acarviostatin-containing aminooligosaccharides derived from Streptomyces sp. HO1518. A total of ninety-eight aminooligosaccharides, including eighty potential new compounds, were detected mainly based on the characteristic fragment ions originating from quinovosidic bond cleavages in their molecules. Following an LC-MS-guided separation technique, seven new aminooligosaccharides (10-16) along with four known related compounds (17-20) were obtained directly from the crude extract of strain HO1518. Compounds 10-13 represent the first examples of aminooligosaccharides with a rare acarviostatin II02-type structure. In addition, all isolates displayed considerable inhibitory effects on three digestive enzymes, which revealed that the number of the pseudo-trisaccharide core(s), the feasible length of the oligosaccharides, and acyl side chain exerted a crucial influence on their bioactivities. These results demonstrated that the UPLC-QTOF-MS/MS-based metabolomics approach could be applied for the rapid identification of aminooligosaccharides and other similar structures in complex samples. Furthermore, this study highlights the potential of acylated aminooligosaccharides with conspicuous alpha-glucosidase and lipase inhibition for the future development of multi-target anti-diabetic drugs. |
| ESTHER : Xu_2022_Mar.Drugs_20_ |
| PubMedSearch : Xu_2022_Mar.Drugs_20_ |
| PubMedID: 35323488 |
Xu J, Liu Z, Feng Z, Ren Y, Liu H, Wang Y (2022)
Rapid Mining of Novel alpha-Glucosidase and Lipase Inhibitors from Streptomyces sp. HO1518 Using UPLC-QTOF-MS\/MS
Mar Drugs
20 :
Xu J, Liu Z, Feng Z, Ren Y, Liu H, Wang Y (2022)
Mar Drugs
20 :