Feng Z

References (26)

Title : Rapid screening of acetylcholinesterase active contaminants in water: A solid phase microextraction-based ligand fishing approach - Huang_2024_Chemosphere_356_141976
Author(s) : Huang Z , He L , Li H , Zhao J , Chen T , Feng Z , Li Y , You J
Ref : Chemosphere , 356 :141976 , 2024
Abstract : Effect-directed analysis (EDA) has been increasingly used for screening toxic contaminants in the environment, but conventional EDA procedures are often time-consuming and labor-extensive. This challenges the use of EDA for toxicant identification in the scenarios when quick answers are demanded. Herein, a solid phase microextraction ligand fishing (SPME-LF) strategy has been proposed as a rapid EDA approach for identifying acetylcholinesterase (AChE) active compounds in water. The feasibility of ligand fishing techniques for screening AChE active chemicals from environmental mixtures was first verified by a membrane separation method. Then, SPME fibers were prepared through self-assembly of boronic acid groups with AChE via co-bonding and applied for SPME-LF. As AChE coated SPME fibers selectively enriched AChE-active compounds from water, comparing sorbing compounds by the SPME fibers with and without AChE coating can quickly distinguish AChE toxicants in mixtures. Compared with conventional EDA, SPME-LF does not require repeating sample separations and bioassays, endowing SPME-LF with the merits of low-cost, labor-saving, and user-friendly. It is believed that cost-efficient and easy-to-use SPME-LF strategy can potentially be a rapid EDA method for screening receptor-specific toxicants in aquatic environment, especially applicable in time-sensitive screening.
ESTHER : Huang_2024_Chemosphere_356_141976
PubMedSearch : Huang_2024_Chemosphere_356_141976
PubMedID: 38608773

Title : Integrative Analysis of Transcriptome and Metabolome to Illuminate the Protective Effects of Didymin against Acute Hepatic Injury - Pang_2023_Mediators.Inflamm_2023_6051946
Author(s) : Pang L , Xiong Y , Feng Z , Li C , Fang B , Huang Q , Lin X
Ref : Mediators Inflamm , 2023 :6051946 , 2023
Abstract : Based on the multiomics analysis, this study is aimed at investigating the underlying mechanism of didymin against acute liver injury (ALI). The mice were administrated with didymin for 2 weeks, followed by injection with lipopolysaccharide (LPS) plus D-galactosamine (D-Gal) to induce ALI. The pathological examination revealed that didymin significantly ameliorated LPS/D-Gal-induced hepatic damage. Also, it markedly reduced proinflammatory cytokines release by inhibiting the TLR4/NF-kappaB pathway activation, alleviating inflammatory injury. A transcriptome analysis proved 2680 differently expressed genes (DEGs) between the model and didymin groups and suggested that the PI3K/Akt and metabolic pathways might be the most relevant targets. Meanwhile, the metabolome analysis revealed 67 differently expressed metabolites (DEMs) between the didymin and model groups that were mainly clustered into the glycerophospholipid metabolism, which was consistent with the transcriptome study. Importantly, a comprehensive analysis of both the omics indicated a strong correlation between the DEGs and DEMs, and an in-depth study demonstrated that didymin alleviated metabolic disorder and hepatocyte injury likely by inhibiting the glycerophospholipid metabolism pathway through the regulation of PLA2G4B, LPCAT3, and CEPT1 expression. In conclusion, this study demonstrates that didymin can ameliorate LPS/D-Gal-induced ALI by inhibiting the glycerophospholipid metabolism and PI3K/Akt and TLR4/NF-kappaB pathways.
ESTHER : Pang_2023_Mediators.Inflamm_2023_6051946
PubMedSearch : Pang_2023_Mediators.Inflamm_2023_6051946
PubMedID: 36687218

Title : Metabolism-guided development of Ko143 analogs as ABCG2 inhibitors - Zhu_2023_Eur.J.Med.Chem_259_115666
Author(s) : Zhu J , Lei S , Lu J , Hao Y , Qian Q , Devanathan AS , Feng Z , Xie XQ , Wipf P , Ma X
Ref : Eur Journal of Medicinal Chemistry , 259 :115666 , 2023
Abstract : ATP-binding cassette subfamily G member 2 (ABCG2), an efflux transporter, is involved in multiple pathological processes. Ko143 is a potent ABCG2 inhibitor; however, it is quickly metabolized through carboxylesterase 1-mediated hydrolysis of its t-butyl ester moiety. The current work aimed to develop more metabolically stable ABCG2 inhibitors. Novel Ko143 analogs were designed and synthesized by replacing the unstable t-butyl ester moiety in Ko143 with an amide group. The synthesized Ko143 analogs were evaluated for their ABCG2 inhibitory activity, binding mode with ABCG2, cytotoxicity, and metabolic stability. We found that the amide modification of Ko143 led to metabolically stable ABCG2 inhibitors. Among these Ko143 analogs, K2 and K34 are promising candidates with favorable oral pharmacokinetic profiles in mice. In summary, we synthesized novel Ko143 analogs with improved metabolic stability, which can potentially be used as lead compounds for the future development of ABCG2 inhibitors.
ESTHER : Zhu_2023_Eur.J.Med.Chem_259_115666
PubMedSearch : Zhu_2023_Eur.J.Med.Chem_259_115666
PubMedID: 37482017

Title : Characterization of Feruloyl Esterase from Klebsiella oxytoca Z28 and Its Application in the Release of Ferulic Acid from De-Starching Wheat Bran - Zhang_2023_Microorganisms_11_989
Author(s) : Zhang Y , Feng Z , Xiang H , Zhang X , Yang L
Ref : Microorganisms , 11 :989 , 2023
Abstract : Feruloyl esterase (EC3.1.1.73; FAE) can degrade biomass to release ferulic acid (FA), which has a high application in bioprocessing, food, pharmaceutical, paper, feed, and other industrial fields. A strain of Klebsiella oxytoca Z28 with ferulic esterase activity was screened from Daqu. In addition, the FAE gene was expressed in Escherichia coli BL21 (DE3). The enzyme consists of 340 amino acids with a molecular mass of 37.7 kDa. The FAE enzyme activity was 463 U/L when the substrate was ethyl 4-hydroxy-3-methoxycinnamate and the optimum temperature and pH were 50 degreesC and 8.0, respectively. The enzyme had good stability at temperatures of 25-40 degreesC and a pH of 8.0. Ba(2+), Cu(2+), Mn(2+), and Ca(2+) had a strong inhibitory effect on the enzyme activity, and Na(+) had a promotive effect on the enzyme activity. The de-starching wheat bran was degraded by KoFAE, and the FA release was up to 227.15 microg/g. This indicated that the heterologous expression of KoFAE from Klebsiella oxytoca Z28 in E. coli had a certain potential of biodegradation, which can be applied to the degradation of agricultural waste to obtain high value-added FA products.
ESTHER : Zhang_2023_Microorganisms_11_989
PubMedSearch : Zhang_2023_Microorganisms_11_989
PubMedID: 37110412
Gene_locus related to this paper: kleox-KoFae

Title : Rapid Mining of Novel alpha-Glucosidase and Lipase Inhibitors from Streptomyces sp. HO1518 Using UPLC-QTOF-MS\/MS - Xu_2022_Mar.Drugs_20_
Author(s) : Xu J , Liu Z , Feng Z , Ren Y , Liu H , Wang Y
Ref : Mar Drugs , 20 : , 2022
Abstract : A rapid and sensitive method using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) was applied for the analysis of the metabolic profile of acarviostatin-containing aminooligosaccharides derived from Streptomyces sp. HO1518. A total of ninety-eight aminooligosaccharides, including eighty potential new compounds, were detected mainly based on the characteristic fragment ions originating from quinovosidic bond cleavages in their molecules. Following an LC-MS-guided separation technique, seven new aminooligosaccharides (10-16) along with four known related compounds (17-20) were obtained directly from the crude extract of strain HO1518. Compounds 10-13 represent the first examples of aminooligosaccharides with a rare acarviostatin II02-type structure. In addition, all isolates displayed considerable inhibitory effects on three digestive enzymes, which revealed that the number of the pseudo-trisaccharide core(s), the feasible length of the oligosaccharides, and acyl side chain exerted a crucial influence on their bioactivities. These results demonstrated that the UPLC-QTOF-MS/MS-based metabolomics approach could be applied for the rapid identification of aminooligosaccharides and other similar structures in complex samples. Furthermore, this study highlights the potential of acylated aminooligosaccharides with conspicuous alpha-glucosidase and lipase inhibition for the future development of multi-target anti-diabetic drugs.
ESTHER : Xu_2022_Mar.Drugs_20_
PubMedSearch : Xu_2022_Mar.Drugs_20_
PubMedID: 35323488

Title : Simultaneous CSM-TACE with CalliSpheres() and partial splenic embolization using 8spheres() for hepatocellular carcinoma with hypersplenism: Early prospective multicenter clinical outcome - Zhou_2022_Front.Oncol_12_998500
Author(s) : Zhou J , Feng Z , Liu S , Li X , Liu Y , Gao F , Shen J , Zhang YW , Zhao GS , Zhang M
Ref : Front Oncol , 12 :998500 , 2022
Abstract : BACKGROUND: Primary hepatocellular carcinoma is often complicated with hepatitis and liver cirrhosis. Some patients develop different degrees of splenomegaly, hypersplenism and hypohepatia due to the aggravation of liver cirrhosis, which to some extent interfere with the treatment of tumors and even affect the prognosis of patients. In this study, we prospectively evaluate the efficacy and safety of simultaneous CalliSpheres((a)) microspheres transcatheter arterial chemoembolization (CSM-TACE) and partial splenic embolization (PSE) using 8spheres((a)) for hepatocellular carcinoma (HCC) with hypersplenism. METHODS: Ninety consecutive HCC patients with hypersplenism who underwent CSM-TACE were selected: 32 patients in CSM-TACE+PSE group, and 58 patients in CSM-TACE group. The peripheral blood cell counts (leukocyte, platelet (PLT), liver function and red blood cell (RBC)), CSM-TACE and/or PSE related complications, and the tumor control rate at 1 month after CSM-TACE were compared. The survival time and prognostic factors were also observed. RESULTS: Before CSM-TACE, there were no significant differences in sex, age, Child-Pugh grade, tumor size, and alpha-fetoprotein (AFP) between the two groups. After CSM-TACE, the PLT and white blood cell (WBC) counts in CSM-TACE+PSE group were significantly higher than those in the CSM-TACE group (P<0.05). There were no significant differences in RBC before and after treatment (P > 0.05). In the CSM-TACE group, there were no significant differences in WBC, PLT, and RBC before and after treatment (P > 0.05). There was no significant difference in liver function at 1 month after treatment between the two groups. The cholinesterase (CHE) level in the CSM-TACE+PSE group after CSM-TACE+PSE was obviously higher than that before CSM-TACE+PSE and higher than that in the CSM-TACE group (P<0.05). However, the level of CHE returned to the preoperative level 1 month after CSM-TACE in the CSM-TACE group. The objective response rate (ORR) and median overall survival (OS) in the CSM-TACE+PSE group were higher than those in the CSM-TACE group (P<0.05). The adverse reactions of the two groups were fever, abdominal pain, stomach discomfort, nausea, and vomiting, and no serious complications occurred. The degree of abdominal pain and fever in the experimental group was lower than that in the control group (P > 0.05). CONCLUSIONS: Simultaneous CSM-TACE and PSE using domestic embolization particles for HCC with hypersplenism have good safety and efficacy and has a low incidence of PSE-related adverse events, it is conducive to improving liver function reserve, and can further improve the median OS.
ESTHER : Zhou_2022_Front.Oncol_12_998500
PubMedSearch : Zhou_2022_Front.Oncol_12_998500
PubMedID: 36530976

Title : Structural and functional insights into the Asp1\/2\/3 complex mediated secretion of pneumococcal serine-rich repeat protein PsrP - Guo_2020_Biochem.Biophys.Res.Commun_524_784
Author(s) : Guo C , Feng Z , Zuo G , Jiang YL , Zhou CZ , Chen Y , Hou WT
Ref : Biochemical & Biophysical Research Communications , 524 :784 , 2020
Abstract : The accessory sec system consisting of seven conserved components is commonly distributed among pathogenic Gram-positive bacteria for the secretion of serine-rich-repeat proteins (SRRPs). Asp1/2/3 protein complex in the system is responsible for both the O-acetylation of GlcNAc and delivering SRRPs to SecA2. However, the molecular mechanism of how Asp1/2/3 transport SRRPs remains unknown. Here, we report the complex structure of Asp1/2/3 from Streptococcus pneumoniae at 2.9 A. Further functional assays indicated that Asp1/2/3 can stimulate the ATPase activity of SecA2. In addition, the deletion of asp1/2/3 gene resulted in the accumulation of a secreted version of PsrP with an altered glycoform in protoplast fraction of the mutant cell, which suggested the modification/transport coupling of the substrate. Altogether, these findings not only provide structural basis for further investigations on the transport process of SRRPs, but also uncover the indispensable role of Asp1/2/3 in the accessory sec system.
ESTHER : Guo_2020_Biochem.Biophys.Res.Commun_524_784
PubMedSearch : Guo_2020_Biochem.Biophys.Res.Commun_524_784
PubMedID: 32037091
Gene_locus related to this paper: stree-a0a0b7mbs7

Title : Lycodine-type alkaloids from Lycopodiastrum casuarinoides and their acetylcholinesterase inhibitory activity - Feng_2019_Fitoterapia__104378
Author(s) : Feng Z , Chen S , Wang W , Feng L , Dong Y , Zou Y , Ke C , Tang C , Yao S , Zhang H , Gan L , Ye Y , Lin L
Ref : Fitoterapia , :104378 , 2019
Abstract : Five previously undescribed lycodine-type alkaloids, named huperzine Y (1), 8,15-epoxy-N-demethylhuperzinine (2), 7-hydroxyl-huperzinine (3), huperzine Z (4), and huperzine D N-oxide (5), were isolated from the whole plants of Lycopodiastrum casuarinoides (Lycopodiaceae), along with ten known analogues. The structures of the new compounds were elucidated by means of spectroscopic technique (IR, UV, MS and NMR). The absolute configurations of the new compounds were established on the basis of comparison of their experimental and TD-DFT (time-dependent density functional theory) calculated ECD spectra. Moreover, all the isolates were evaluated for acetylcholinesterase (AChE) inhibitory activity. Only huperzine C showed moderate activity, with an IC50 value of 0.525+/-0.140muM, which was comparable with the positive control, huperzine A (IC50=0.143+/-0.029muM).
ESTHER : Feng_2019_Fitoterapia__104378
PubMedSearch : Feng_2019_Fitoterapia__104378
PubMedID: 31676395

Title : Genomic Analysis of Microbulbifer sp. Strain A4B-17 and the Characterization of Its Metabolic Pathways for 4-Hydroxybenzoic Acid Synthesis - Tian_2018_Front.Microbiol_9_3115
Author(s) : Tian J , Zhu L , Wang W , Zhang L , Li Z , Zhao Q , Xing K , Feng Z , Peng X
Ref : Front Microbiol , 9 :3115 , 2018
Abstract : The marine bacterium Microbulbifer sp. A4B-17 produces secondary metabolites such as 4-hydroxybenzoic acid (4HBA) and esters of 4HBA (parabens). 4HBA is a useful material in the synthesis of the liquid crystal. Parabens are man-made compounds that have been extensively used since the 1920s in the cosmetic, pharmaceutical, and food industries for their effective antimicrobial activity. In this study, we completed the sequencing and annotation of the A4B-17 strain genome and found all genes for glucose utilization and 4HBA biosynthesis. Strain A4B-17 uses the Embden-Meyerhof-Parnas (EMP), hexose monophosphate (HMP), and Entner-Doudoroff (ED) pathways to utilize glucose. Other sugars such as fructose, sucrose, xylose, arabinose, galactose, mannitol, and glycerol supported cell growth and 4HBA synthesis. Reverse transcriptional analysis confirmed that the key genes involved in the glucose metabolism were functional. Paraben concentrations were proportionally increased by adding alcohols to the culture medium, indicating that strain A4B-17 synthesizes the 4HBA and the alcohols separately and an esterification reaction between them is responsible for the paraben synthesis. A gene that codes for a carboxylesterase was proposed to catalyze this reaction. The temperature and NaCl concentration for optimal growth were determined to be 35 degrees C and 22.8 g/L.
ESTHER : Tian_2018_Front.Microbiol_9_3115
PubMedSearch : Tian_2018_Front.Microbiol_9_3115
PubMedID: 30619190

Title : Acetylcholinesterase Biosensor Based On Mesoporous Hollow Carbon Spheres\/Core-Shell Magnetic Nanoparticles-Modified Electrode for the Detection of Organophosphorus Pesticides - Luo_2018_Sensors.(Basel)_18_
Author(s) : Luo R , Feng Z , Shen G , Xiu Y , Zhou Y , Niu X , Wang H
Ref : Sensors (Basel) , 18 : , 2018
Abstract : The present study investigated the synthesis of mesoporous hollow carbon spheres (MHCS) and magnetic mesoporous hollow carbon spheres with core-shell structures (Fe(3)O(4)@MHCS). Two acetylcholinesterase sensors (acetylcholinesterase/mesoporous hollow carbon spheres/glassy carbon electrode (AChE/MHCS/GCE) and acetylcholinesterase/core-shell magnetic mesoporous hollow carbon spheres/glassy carbon electrode (AChE/Fe(3)O(4)@MHCS/GCE) based on mesoporous carbon materials were prepared. Under the optimum conditions, using Malathion as the model compound, the developed biosensors showed a wide detection range, low detection limit, good reproducibility, and high stability. The AChE/MHCS/GCE electrochemical sensor response exhibited two good linear ranges at the incubation time of 10 min at the Malathion concentration ranges of 0.01 to 100 ppb and 100 to 600 ppb, with a detection limit of 0.0148 ppb (S/N = 3). The AChE/Fe(3)O(4)@MHCS/GCE electrochemical sensor that was operated with an incubation time of 12 min at the malathion concentration ranges between 0.01(-)50 ppb and 50(-)600 ppb had a detection limit of 0.0182 ppb (S/N = 3). Moreover, the AChE/MHCS/GCE and AChE/Fe(3)O(4)@MHCS/GCE biosensors were effective for the detection of real samples, and were demonstrated to be suitable for the field-testing of organophosphorus pesticide (OP) residues.
ESTHER : Luo_2018_Sensors.(Basel)_18_
PubMedSearch : Luo_2018_Sensors.(Basel)_18_
PubMedID: 30558201

Title : Synergism of antihypertensives and cholinesterase inhibitors in Alzheimer's disease - Hu_2018_Alzheimers.Dement.(N.Y)_4_542
Author(s) : Hu Z , Wang L , Ma S , Kirisci L , Feng Z , Xue Y , Klunk WE , Kamboh MI , Sweet RA , Becker J , Lv Q , Lopez OL , Xie XQ
Ref : Alzheimers Dement (N Y) , 4 :542 , 2018
Abstract : Introduction: We investigated the effect of antihypertensive (aHTN) medications and cholinesterase inhibitors (ChEIs) on the cognitive decline in patients with Alzheimer's disease (AD) and analyzed synergism by chemogenomics systems pharmacology mapping. Methods: We compared the effect of aHTN drugs on Mini-Mental State Examination scores in 617 AD patients with hypertension, and studied the synergistic effects. Results: The combination of diuretics, calcium channel blockers, and renin-angiotensin-aldosterone system blockers showed slower cognitive decline compared with other aHTN groups (Deltabeta = +1.46, P < .0001). aHTN medications slow down cognitive decline in ChEI users (Deltabeta = +0.56, P = .006), but not in non-ChEI users (Deltabeta = -0.31, P = .53). Discussion: aHTN and ChEI drugs showed synergistic effects. A combination of diuretics, renin-angiotensin-aldosterone system blockers, and calcium channel blockers had the slowest cognitive decline. The chemogenomics systems pharmacology-identified molecular targets provide system pharmacology interpretation of the synergism of the drugs in clinics. The results suggest that improving vascular health is essential for AD treatment and provide a novel direction for AD drug development.
ESTHER : Hu_2018_Alzheimers.Dement.(N.Y)_4_542
PubMedSearch : Hu_2018_Alzheimers.Dement.(N.Y)_4_542
PubMedID: 30386819

Title : O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3 - Wu_2017_J.Biol.Chem_292_10014
Author(s) : Wu D , Zhao L , Feng Z , Yu C , Ding J , Wang L , Wang F , Liu D , Zhu H , Xing F , Conaway JW , Conaway RC , Cai Y , Jin J
Ref : Journal of Biological Chemistry , 292 :10014 , 2017
Abstract : The human males absent on the first (MOF)-containing histone acetyltransferase nonspecific lethal (NSL) complex comprises nine subunits including the O-linked N-acetylglucosamine (O-GlcNAc) transferase, isoform 1 (OGT1). However, whether the O-GlcNAc transferase activity of OGT1 controls histone acetyltransferase activity of the NSL complex and whether OGT1 physically interacts with the other NSL complex subunits remain unclear. Here, we demonstrate that OGT1 regulates the activity of the NSL complex by mainly acetylating histone H4 Lys-16, Lys-5, and Lys-8 via O-GlcNAcylation and stabilization of the NSL complex subunit NSL3. Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. Because OGT1 is a subunit of the NSL complex, we also investigated the function of OGT1 in this complex. Co-transfection/co-immunoprecipitation experiments combined with in vitro O-GlcNAc transferase assays confirmed that OGT1 specifically binds to and O-GlcNAcylates NSL3. In addition, wheat germ agglutinin affinity purification verified the occurrence of O-GlcNAc modification on NSL3 in cells. Moreover, O-GlcNAcylation of NSL3 by wild-type OGT1 (OGT1-WT) stabilized NSL3. This stabilization was lost after co-transfection of NSL3 with an OGT1 mutant, OGT1(C964A), that lacks O-GlcNAc transferase activity. Furthermore, stabilization of NSL3 by OGT1-WT significantly increased the global acetylation levels of H4 Lys-5, Lys-8, and Lys-16 in cells. These results suggest that OGT1 regulates the activity of the NSL complex by stabilizing NSL3.
ESTHER : Wu_2017_J.Biol.Chem_292_10014
PubMedSearch : Wu_2017_J.Biol.Chem_292_10014
PubMedID: 28450392
Gene_locus related to this paper: human-KANSL3

Title : Identification and Characterization of Two Novel Esterases from a Metagenomic Library - Gu_2015_Food.Sci.Technol.Res_21_649
Author(s) : Gu X , Wang S , Wang SC , Zhao LX , Cao M , Feng Z
Ref : Food Science and Technology Research , 21 :649 , 2015
Abstract : Esterases are biocatalysts in food industry aimed for nutrition improvements, formation of flavor, and food fermentation. Two esterases EstGX1 and EstGX2 were identified based on function-based screening of a soil metagenomic cosmid library. Enzyme properties including optimum pH, optimal temperature, tolerance to organic solvents and metal ions were measured, respectively. The activity of EstGX2 could maintain about 40% after incubated at 99C for 55 min, and could be increased in presence of 15% ethanol. The unique properties of EstGX2, high thermostability and stability in the presence of several organic solvents, may make it a promising enzyme candidate in food industry.
ESTHER : Gu_2015_Food.Sci.Technol.Res_21_649
PubMedSearch : Gu_2015_Food.Sci.Technol.Res_21_649
Gene_locus related to this paper: 9bact-estGX1 , 9bact-a0a0d5w6z2

Title : Human pDCs preferentially sense enveloped hepatitis A virions - Feng_2015_J.Clin.Invest_125_169
Author(s) : Feng Z , Li Y , McKnight KL , Hensley L , Lanford RE , Walker CM , Lemon SM
Ref : J Clinical Investigation , 125 :169 , 2015
Abstract : Unlike other picornaviruses, hepatitis A virus (HAV) is cloaked in host membranes when released from cells, providing protection from neutralizing antibodies and facilitating spread in the liver. Acute HAV infection is typified by minimal type I IFN responses; therefore, we questioned whether plasmacytoid dendritic cells (pDCs), which produce IFN when activated, are capable of sensing enveloped virions (eHAV). Although concentrated nonenveloped virus failed to activate freshly isolated human pDCs, these cells produced substantial amounts of IFN-alpha via TLR7 signaling when cocultured with infected cells. pDCs required either close contact with infected cells or exposure to concentrated culture supernatants for IFN-alpha production. In isopycnic and rate-zonal gradients, pDC-activating material cosedimented with eHAV but not membrane-bound acetylcholinesterase, suggesting that eHAV, and not viral RNA exosomes, is responsible for IFN-alpha induction. pDC activation did not require virus replication and was associated with efficient eHAV uptake, which was facilitated by phosphatidylserine receptors on pDCs. In chimpanzees, pDCs were transiently recruited to the liver early in infection, during or shortly before maximal intrahepatic IFN-stimulated gene expression, but disappeared prior to inflammation onset. Our data reveal that, while membrane envelopment protects HAV against neutralizing antibody, it also facilitates an early but limited detection of HAV infection by pDCs.
ESTHER : Feng_2015_J.Clin.Invest_125_169
PubMedSearch : Feng_2015_J.Clin.Invest_125_169
PubMedID: 25415438

Title : FAM172A induces S phase arrest of HepG2 cells via Notch 3 - Feng_2013_Oncol.Rep_29_1154
Author(s) : Feng Z , Li H , Liu S , Cheng J , Xiang G , Zhang J
Ref : Oncol Rep , 29 :1154 , 2013
Abstract : Our previous results revealed that FAM172A was significantly downregulated in liver tissue from hepatocellular carcinoma or cirrhotic patients. The present study was designed to elucidate the regulatory role of FAM172A in HepG2 cells. In order to determine the expression of the FAM172A protein, western blot analysis was performed. Confocal laser scanning technique was used to observe the localization of FAM172A in HepG2 cells. Surface plasmon resonance experiments were used to determine the binding activity of FAM172A and active single sugar and Ca2+. The cell cycle progression of HepG2 cells was assessed by flow cytometry. The FAM172A protein was localized in the endoplasmic reticulum of HepG2 cells. This protein was moderately expressed in normal liver tissue, but was significantly decreased in liver tissue of patients with chronic hepatitis B When co-cultured with the FAM172A recombinant protein, HepG2 cells exhibited complete cell cycle arrest in the S phase at a high concentration (100 ng/ml). Proliferation of HepG2 cells treated with the FAM172A recombinant protein was prominently inhibited compared with that of the control cells. Western blot analysis showed that upregulation of Notch 3 and cyclin E may be related with the cell cycle control. Our results indicate that FAM172A may be a novel tumor-suppressor gene, which plays an important role in cell cycle control and tumor cell proliferation. G1/S phase arrest may be mediated, at least partially, by the Notch 3 signaling pathway.
ESTHER : Feng_2013_Oncol.Rep_29_1154
PubMedSearch : Feng_2013_Oncol.Rep_29_1154
PubMedID: 23314443

Title : Metabonomics analysis of urine and plasma from rats given long-term and low-dose dimethoate by ultra-performance liquid chromatography-mass spectrometry - Feng_2012_Chem.Biol.Interact_199_143
Author(s) : Feng Z , Sun X , Yang J , Hao D , Du L , Wang H , Xu W , Zhao X , Sun C
Ref : Chemico-Biological Interactions , 199 :143 , 2012
Abstract : This study assessed the effects of long-term low-dose dimethoate administration to rats by ultra-performance liquid chromatography-mass spectrometry UPLC-MS Dimethoate 0.04 0.12 and 0.36mg/kg body weight/day was administered daily to male Wistar rats through their drinking water for 24weeks Significant changes in serum clinical chemistry were observed in the middle and high-dose groups UPLC-MS revealed evident separate clustering among the different dose groups using global metabolic profiling by supervised partial least squares-discriminant analysis Metabonomic analysis showed alterations in a number of metabolites 12 from urine and 13 from plasma such as l-tyrosine dimethylthiophosphate DMTP dimethyldithiophosphate DMDTP citric acid uric acid suberic acid glycylproline allantoin isovalerylglutamic acid and kinds of lipids The results suggest that long-term low-dose exposure to dimethoate can cause disturbances in liver function antioxidant and nervous systems as well as the metabolisms of lipids glucose fatty acids amino acids and collagen in rats DMTP and DMDTP which had the most significant changes among all other studied biomarkers were considered as early sensitive biomarkers of exposure to dimethoate The other aforementioned proposed toxicity biomarkers in metabonomic analysis may be useful in the risk assessment of the toxic effects of dimethoate Metabonomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of dimethoate induced toxicity In addition the results indicate that metabonomic approach could detect systemic toxic effects at an earlier stage compared to clinical chemistry The combination of metabonomics and clinical chemistry made the toxicity of dimethoate on rats more comprehensive.
ESTHER : Feng_2012_Chem.Biol.Interact_199_143
PubMedSearch : Feng_2012_Chem.Biol.Interact_199_143
PubMedID: 22884955

Title : Complete genome sequence of Mycoplasma hyopneumoniae strain 168 - Liu_2011_J.Bacteriol_193_1016
Author(s) : Liu W , Feng Z , Fang L , Zhou Z , Li Q , Li S , Luo R , Wang L , Chen H , Shao G , Xiao S
Ref : Journal of Bacteriology , 193 :1016 , 2011
Abstract : Mycoplasma hyopneumoniae strain 168, a pathogenic strain prevalent in China, was isolated in 1974. Although this strain has been widespread for a long time, the genome sequence had not been determined. Here, we announce the complete genome sequence of M. hyopneumoniae strain 168.
ESTHER : Liu_2011_J.Bacteriol_193_1016
PubMedSearch : Liu_2011_J.Bacteriol_193_1016
PubMedID: 21148737

Title : Metabolomic analysis of the toxic effects of chronic exposure to low-level dichlorvos on rats using ultra-performance liquid chromatography-mass spectrometry - Yang_2011_Toxicol.Lett_206_306
Author(s) : Yang J , Sun X , Feng Z , Hao D , Wang M , Zhao X , Sun C
Ref : Toxicol Lett , 206 :306 , 2011
Abstract : The purpose of the current study was to assess the effects of long-term exposure to low levels of DDVP on the biochemical parameters and metabolic profiles of rats. Three different doses (2.4, 7.2, and 21.6 mg/kg body weight/day) of DDVP were administered to rats through their drinking water over 24 weeks. Significant changes in blood cholinesterase, creatinine, urea nitrogen, aspartate aminotransferase, alanine aminotransferase, and albumin concentrations were observed in the middle and high dose groups. Changes in the concentration of some urine metabolites were detected via ultra performance liquid chromatography-mass spectrometry (UPLC-MS). Dimethyl phosphate (DMP), which was exclusively detected in the treated groups, can be an early, sensitive biomarker for DDVP exposure. Moreover, DDVP treatment resulted in an increase in the lactobionic acid, estrone sulfate, and indoxyl sulfic concentrations, and a decrease in citric acid, suberic acid, gulonic acid, urea, creatinine, and uric acid. These results suggest that chronic exposure to low-level DDVP can cause a disturbance in carbohydrate and fatty acid metabolism, the antioxidant system, etc. Therefore, an analysis of the metabolic profiles can contribute to the understanding of the adverse effects of long-term exposure to low doses of DDVP.
ESTHER : Yang_2011_Toxicol.Lett_206_306
PubMedSearch : Yang_2011_Toxicol.Lett_206_306
PubMedID: 21889581

Title : Complete genome sequence of Mycoplasma hyorhinis strain HUB-1 - Liu_2010_J.Bacteriol_192_5844
Author(s) : Liu W , Fang L , Li S , Li Q , Zhou Z , Feng Z , Luo R , Shao G , Wang L , Chen H , Xiao S
Ref : Journal of Bacteriology , 192 :5844 , 2010
Abstract : Mycoplasma hyorhinis is generally considered a swine pathogen yet is most commonly found infecting laboratory cell lines. An increasing body of evidence suggests that chronic infections with M. hyorhinis may cause oncogenic transformation. Here, we announce the complete genome sequence of M. hyorhinis strain HUB-1.
ESTHER : Liu_2010_J.Bacteriol_192_5844
PubMedSearch : Liu_2010_J.Bacteriol_192_5844
PubMedID: 20802032

Title : Design and synthesis of prolylcarboxypeptidase (PrCP) inhibitors to validate PrCP as a potential target for obesity - Zhou_2010_J.Med.Chem_53_7251
Author(s) : Zhou C , Garcia-Calvo M , Pinto S , Lombardo M , Feng Z , Bender K , Pryor KD , Bhatt UR , Chabin RM , Geissler WM , Shen Z , Tong X , Zhang Z , Wong KK , Roy RS , Chapman KT , Yang L , Xiong Y
Ref : Journal of Medicinal Chemistry , 53 :7251 , 2010
Abstract : Prolylcarboxypeptidase (PrCP) is a serine protease that may have a role in metabolism regulation. A class of reversible, potent, and selective PrCP inhibitors was developed starting from a mechanism based design for inhibiting this serine protease. Compound 8o inhibits human and mouse PrCP at IC(50) values of 1 and 2 nM and is not active (IC(50) > 25 microM) against a panel of closely related proteases. It has lower serum binding than its close analogues and is bioavailable in mouse. Subchronic dosing of 8o in PrCP(-/-) and WT mice at 100 mg/kg for 5 days resulted in a 5% reduction in body weight in WT mice and a 1% reduction in PrCP KO mice.
ESTHER : Zhou_2010_J.Med.Chem_53_7251
PubMedSearch : Zhou_2010_J.Med.Chem_53_7251
PubMedID: 20857914
Gene_locus related to this paper: human-PRCP

Title : Cloning large natural product gene clusters from the environment: piecing environmental DNA gene clusters back together with TAR - Kim_2010_Biopolymers_93_833
Author(s) : Kim JH , Feng Z , Bauer JD , Kallifidas D , Calle PY , Brady SF
Ref : Biopolymers , 93 :833 , 2010
Abstract : A single gram of soil can contain thousands of unique bacterial species, of which only a small fraction is regularly cultured in the laboratory. Although the fermentation of cultured microorganisms has provided access to numerous bioactive secondary metabolites, with these same methods it is not possible to characterize the natural products encoded by the uncultured majority. The heterologous expression of biosynthetic gene clusters cloned from DNA extracted directly from environmental samples (eDNA) has the potential to provide access to the chemical diversity encoded in the genomes of uncultured bacteria. One of the challenges facing this approach has been that many natural product biosynthetic gene clusters are too large to be readily captured on a single fragment of cloned eDNA. The reassembly of large eDNA-derived natural product gene clusters from collections of smaller overlapping clones represents one potential solution to this problem. Unfortunately, traditional methods for the assembly of large DNA sequences from multiple overlapping clones can be technically challenging. Here we present a general experimental framework that permits the recovery of large natural product biosynthetic gene clusters on overlapping soil-derived eDNA cosmid clones and the reassembly of these large gene clusters using transformation-associated recombination (TAR) in Saccharomyces cerevisiae. The development of practical methods for the rapid assembly of biosynthetic gene clusters from collections of overlapping eDNA clones is an important step toward being able to functionally study larger natural product gene clusters from uncultured bacteria.
ESTHER : Kim_2010_Biopolymers_93_833
PubMedSearch : Kim_2010_Biopolymers_93_833
PubMedID: 20577994
Gene_locus related to this paper: 9bact-e2d2h0

Title : Genomic differences of Vaccinia virus clones from Dryvax smallpox vaccine: the Dryvax-like ACAM2000 and the mouse neurovirulent Clone-3 - Osborne_2007_Vaccine_25_8807
Author(s) : Osborne JD , Da Silva M , Frace AM , Sammons SA , Olsen-Rasmussen M , Upton C , Buller RM , Chen N , Feng Z , Roper RL , Liu J , Pougatcheva S , Chen W , Wohlhueter RM , Esposito JJ
Ref : Vaccine , 25 :8807 , 2007
Abstract : Conventional vaccines used for smallpox eradication were often denoted one or another strain of Vaccinia virus (VACV), even though seed virus was sub-cultured multifariously, which rendered the virion population genetically heterogeneous. ACAM2000 cell culture vaccine, recently licensed in the U.S., consists of a biologically vaccine-like VACV homogeneous-sequence clone from the conventional smallpox vaccine Dryvax, which we verified from Dryvax sequence chromatograms is genetically heterogeneous. ACAM2000 VACV and CL3, a mouse-neurovirulent clone from Dryvax, differ by 572 single nucleotide polymorphisms and 53 insertions-deletions of varied size, including a 4.5-kbp deletion in ACAM2000 and a 6.2-kbp deletion in CL3. The sequence diversity between the two clones precludes precisely defining why CL3 is more pathogenic; however, four genes appear significantly dissimilar to account for virulence differences. CL3 encodes intact immunomodulators interferon-alpha/beta and tumor necrosis factor receptors, which are truncated in ACAM2000. CL3 specifies a Cowpox and Variola virus-like ankyrin-repeat protein that might be associated with proteolysis via ubiquitination. And, CL3 shows an elongated thymidylate kinase, similar to the enzyme of the mouse-neurovirulent VACV-WR, a derivative of the New York City Board of Health vaccine, the origin vaccine of Dryvax. Although ACAM2000 encodes most proteins associated with immunization protection, the cloning probably delimited the variant epitopes and other motifs produced by Dryvax due to its VACV genetic heterogeneity. The sequence information for ACAM2000 and CL3 could be significant for resolving the dynamics of their different proteomes and thereby aid development of safer, more effective vaccines.
ESTHER : Osborne_2007_Vaccine_25_8807
PubMedSearch : Osborne_2007_Vaccine_25_8807
PubMedID: 18037545
Gene_locus related to this paper: cowvi-M5L

Title : New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum - Liu_2006_PLoS.Pathog_2_e29
Author(s) : Liu F , Lu J , Hu W , Wang SY , Cui SJ , Chi M , Yan Q , Wang XR , Song HD , Xu XN , Wang JJ , Zhang XL , Zhang X , Wang ZQ , Xue CL , Brindley PJ , McManus DP , Yang PY , Feng Z , Chen Z , Han ZG
Ref : PLoS Pathog , 2 :e29 , 2006
Abstract : Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.
ESTHER : Liu_2006_PLoS.Pathog_2_e29
PubMedSearch : Liu_2006_PLoS.Pathog_2_e29
PubMedID: 16617374
Gene_locus related to this paper: schja-q5byv1

Title : Long-term effects of melatonin or 17 beta-estradiol on improving spatial memory performance in cognitively impaired, ovariectomized adult rats - Feng_2004_J.Pineal.Res_37_198
Author(s) : Feng Z , Cheng Y , Zhang JT
Ref : J Pineal Res , 37 :198 , 2004
Abstract : Melatonin is an endogenously generated potent antioxidant. Our previous studies indicate that melatonin improved learning and memory deficits in APP695 transgenic mouse of Alzheimer's disease. An ovariectomized (OVX) rat model which is characterized by progressive memory deficits, central cholinergic nerve system degeneration and differentiation/apoptosis imbalance is the ideal in vivo model in which to test the neuroprotective effects of melatonin. OVX Sprague-Dawley rats received daily injections of melatonin (5, 10 and 20 mg/kg) or 17 beta-estradiol (E2, 80 microg/kg) or sesame oil for 16 wk. Morris water maze results showed that ovarian steroid deprivation resulted in spatial memory impairment, while melatonin and E2 significantly ameliorated spatial memory deficits in OVX rats. The latency to find the hidden platform and the distance to reach the platform become shorter in both melatonin and E2-treated rats compared with those that were only OVX. Four months after OVX, the choline acetyltransferase activity in the frontal cortex and hippocampus were greatly decreased in comparison with the controls. Melatonin and E2 antagonized the effects induced by OVX. Interestingly, the activity of the acetylcholinesterase was not altered in any group of rats. DNA fragmentation was presented in the front cortex of the OVX rats. Melatonin and E2 reduced the number of apoptotic neurons. These findings demonstrate the important effects of melatonin and E2 on cholinergic neurons and support the potential application of melatonin in the treatment of dementia in postmenopausal women. Our results indicate that neuroprotection by melatonin partly correlated to modulation of apoptosis and protection of the cholinergic system. Early long-term melatonin application is a promising strategy which could potentially be applied in a clinic setting.
ESTHER : Feng_2004_J.Pineal.Res_37_198
PubMedSearch : Feng_2004_J.Pineal.Res_37_198
PubMedID: 15357665

Title : [Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats] - Wang_2003_Zhonghua.Shao.Shang.Za.Zhi_19_275
Author(s) : Wang XJ , Sun YH , Ding QX , Feng Z , Cao Y
Ref : Zhonghua Shao Shang Za Zhi , 19 :275 , 2003
Abstract : OBJECTIVE: To explore the pathogenesis of postburn intestinal mucosal injury in scalded rats. METHODS: The rats inflicted with full thickness burn were employed as the model. The two-dimensional electrophoresis (2-DE) was employed to identify the down-regulating proteins from the differential proteins in scalded rats. Spot detection and matching were performed with Image Master 2D Elite. Mass spectrometry was performed on Bruker BIFLEX III TOF. RESULTS: There are 34 points of proteins in intestinal mucosa in scalded rats which were down-regulated at 6 and 12 postburn hours. Among them 22 proteins were employed for the identification and analysis. Mitochondrial aconitase, alpha-propionyl -CoA carboxylase heavy chain A of F1-ATPase in rat liver, Troponin short-chain of hydroxyacyl-coenzyme A dehydrogenase, alpha-subunit of P-electronic transferring flavoprotein (ETF) were down-regulating proteins correlated with mitochondria in intestinal mucosa in severely scalded rats. Triosephosphate isomerase 1 and cytosolic epoxide hydrolase were down-regulating proteins participated in metabolism of scalded rats. Fibromodulin, dynein-like protein, Troponin-2 and myosin light chain 3 alkali (MLC) were down-regulating proteins correlated with cellular skeletal protein. Glucocorticoid-inducible protein, nuclear factor1-B2, BRCA1, transcriptive factor EB (estradiol benzoate), beta2 subunit of G-protein, N-methyl-D-aspartate receptor1 (NMDAR) were down-regulating proteins participated in postburn regulation of intestinal mucosa in scalded rats. T-cell receptor-V-delta 6 and Ig heavy chain V region protein 1 were down-regulating proteins correlated with the immunomodulation of intestinal mucosa in scalded rats. CONCLUSION: The down-regulating proteins of intestinal mucosa in scalded rats exhibited close relationship with mitochondria.
ESTHER : Wang_2003_Zhonghua.Shao.Shang.Za.Zhi_19_275
PubMedSearch : Wang_2003_Zhonghua.Shao.Shang.Za.Zhi_19_275
PubMedID: 14687529

Title : Evolutionary and biomedical implications of a Schistosoma japonicum complementary DNA resource - Hu_2003_Nat.Genet_35_139
Author(s) : Hu W , Yan Q , Shen DK , Liu F , Zhu ZD , Song HD , Xu XR , Wang ZJ , Rong YP , Zeng LC , Wu J , Zhang X , Wang JJ , Xu XN , Wang SY , Fu G , Zhang XL , Wang ZQ , Brindley PJ , McManus DP , Xue CL , Feng Z , Chen Z , Han ZG
Ref : Nat Genet , 35 :139 , 2003
Abstract : Schistosoma japonicum causes schistosomiasis in humans and livestock in the Asia-Pacific region. Knowledge of the genome of this parasite should improve understanding of schistosome-host interactions, biomedical aspects of schistosomiasis and invertebrate evolution. We assigned 43,707 expressed sequence tags (ESTs) derived from adult S. japonicum and their eggs to 13,131 gene clusters. Of these, 35% shared no similarity with known genes and 75% had not been reported previously in schistosomes. Notably, S. japonicum encoded mammalian-like receptors for insulin, progesterone, cytokines and neuropeptides, suggesting that host hormones, or endogenous parasite homologs, could orchestrate schistosome development and maturation and that schistosomes modulate anti-parasite immune responses through inhibitors, molecular mimicry and other evasion strategies.
ESTHER : Hu_2003_Nat.Genet_35_139
PubMedSearch : Hu_2003_Nat.Genet_35_139
PubMedID: 12973349
Gene_locus related to this paper: schja-Q86EA4 , schja-Q86EV0 , schja-Q86F58 , schja-Q86F66 , schja-Q5DDP9