Yang_2023_J.Hazard.Mater_464_132965

Reference

Title : Remodeling the polymer-binding cavity to improve the efficacy of PBAT-degrading enzyme - Yang_2023_J.Hazard.Mater_464_132965
Author(s) : Yang Y , Cheng S , Zheng Y , Xue T , Huang JW , Zhang L , Guo RT , Chen CC
Ref : J Hazard Mater , 464 :132965 , 2023
Abstract :

Poly(butylene adipate-co-terephthalate) (PBAT) is among the most widely applied synthetic polyesters that are utilized in the packaging and agricultural industries, but the accumulation of PBAT wastes has posed a great burden to ecosystems. Using renewable enzymes to decompose PBAT is an eco-friendly solution to tackle this problem. Recently, we demonstrated that cutinase is the most effective PBAT-degrading enzyme and that an engineered cutinase termed TfCut-DM could completely decompose PBAT film to terephthalate (TPA). Here, we report crystal structures of a variant of leaf compost cutinase in complex with soluble fragments of PBAT, including BTa and TaBTa. In the TaBTa complex, one TPA moiety was located at a polymer-binding site distal to the catalytic center that has never been experimentally validated. Intriguingly, the composition of the distal TPA-binding site shows higher diversity relative to the one proximal to the catalytic center in various cutinases. We thus modified the distal TPA-binding site of TfCut-DM and obtained variants that exhibit higher activity. Notably, the time needed to completely degrade the PBAT film to TPA was shortened to within 24 h by TfCut-DM Q132Y (5813 mol per mol protein). Taken together, the structural information regarding the substrate-binding behavior of PBAT-degrading enzymes could be useful guidance for direct enzyme engineering.

PubMedSearch : Yang_2023_J.Hazard.Mater_464_132965
PubMedID: 37979420
Gene_locus related to this paper: 9bact-g9by57

Related information

Substrate 1,4-butanediol-terephthalate    BTa    PBAT
Gene_locus 1,4-butanediol-terephthalate    BTa    PBAT    9bact-g9by57
Structure 1,4-butanediol-terephthalate    BTa    PBAT    9bact-g9by57    8JMP    8JMO

Citations formats

Yang Y, Cheng S, Zheng Y, Xue T, Huang JW, Zhang L, Guo RT, Chen CC (2023)
Remodeling the polymer-binding cavity to improve the efficacy of PBAT-degrading enzyme
J Hazard Mater 464 :132965

Yang Y, Cheng S, Zheng Y, Xue T, Huang JW, Zhang L, Guo RT, Chen CC (2023)
J Hazard Mater 464 :132965