Yucel_2014_Dev.Biol_392_266

Reference

Title : SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans - Yucel_2014_Dev.Biol_392_266
Author(s) : Yucel D , Hoe M , Llamosas E , Kant S , Jamieson C , Young PA , Crossley M , Nicholas HR
Ref : Developmental Biology , 392 :266 , 2014
Abstract :

Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval development in C. elegans and the proteins encoded by these genes include components of several histone modification and chromatin remodelling complexes. By inhibiting ectopic expression of the epidermal growth factor (LIN-3) in the nematode hypodermis, the synMuv genes prevent inappropriate vulval induction. In a forward genetic screen for modifiers of the expression of a hypodermal reporter gene, we identified a mutation that results in increased expression of the reporter. This mutation also suppresses ectopic vulval induction in synMuv mutants and we have consequently named the affected gene suppressor of synthetic multivulva-1 (sumv-1). We show that SUMV-1 is required in the hypodermis for the synMuv phenotype and that loss of sumv-1 function suppresses ectopic expression of lin-3 in synMuv mutant animals. In yeast two-hybrid assays SUMV-1 physically interacts with SUMV-2, and reduction of sumv-2 function also suppresses the synMuv phenotype. We identified similarities between SUMV-1 and SUMV-2 and mammalian proteins KAT8 NSL2 and KAT8 NSL3, respectively, which are components of the KAT8/MOF histone acetyltransferase complex. Reduction of function of mys-2, which encodes the enzymatic component of the KAT8/MOF complex, also suppresses the synMuv phenotype, and MYS-2 physically interacts with SUMV-2 in yeast two-hybrid assays. Together these observations suggest that SUMV-1 and SUMV-2 may function together with MYS-2 in a nematode KAT8/MOF-like complex to antagonise the activity of the synMuv genes.

PubMedSearch : Yucel_2014_Dev.Biol_392_266
PubMedID: 24882710
Gene_locus related to this paper: caeel-q22992

Related information

Gene_locus caeel-q22992
Family caeel-q22992    NLS3-Tex30

Citations formats

Yucel D, Hoe M, Llamosas E, Kant S, Jamieson C, Young PA, Crossley M, Nicholas HR (2014)
SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans
Developmental Biology 392 :266

Yucel D, Hoe M, Llamosas E, Kant S, Jamieson C, Young PA, Crossley M, Nicholas HR (2014)
Developmental Biology 392 :266