Family: NLS3-Tex30

Block: X

Parent Family: No family


This entry consists of the KAT8 regulatory NSL complex subunit 3 (KANSL3 or NSL3, also known as testis development protein PRTD) and the testis-expressed sequence 30 proteins (TEX30). KAT8 regulatory NSL complex subunit 3 is part of the NSL complex that is involved in acetylation of nucleosomal histone H4 on several lysine residues and therefore may be involved in the regulation of transcription. Meunier et al identified KANSL3 as a microtubule minus-end-binding protein. In C. Elegans SUMV-2 interacts with SUMV-1, and they may function together with MYS-2 in a nematode KAT8/MOF-like complex to antagonise the activity of the synMuv (synthetic multivulva) genes. The function of the testis-expressed sequence 30 protein is not known. Wu et al. showed that KANSL3 is a subunit of a nonspecific lethal (NSL) histone acetyltransferase (HAT) complex that acetylates core histone H4. NSL3 and the O-linked N-acetylglucosamine (O-GlcNAc) transferase OGT1 are subunits of an NSL HAT complex that acetylates histone H4 residues lys5 (K5), K8, and K16. Knockdown of OGT1 or NSL3 reduced global H4K16 acetylation in a dose-dependent manner. Immunoprecipitation analysis in HEK293 and HeLa cells revealed that endogenous OGT1 and NSL3 interacted, and mutation analysis revealed that the N-terminal TPR repeat domain of OGT1 interacted with the abhydrolase domain of NSL3. In vitro, OGT1 catalyzed O-GlcNAc modification of NSL3 in the presence of donor UDP-GlcNAc. The O-GlcNAc modification stabilized NSL3, and stabilized NSL3 was required for H4K16 acetylation by the HAT NSL complex


Interpro : IPR026555 KAT8 regulatory NSL complex subunit 3\/Testis-expressed sequence 30 protein , IPR046879 KANL3\/Tex30, alpha\/beta hydrolase-like domain


Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

No structure scheme yet for this family

Genes Proteins in NLS3-Tex30 family (676)

Fragments of genes in NLS3-Tex30 family (58)

No fragment of genes

No substrate

No Inhibitor

References (4)

Title : O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3 - Wu_2017_J.Biol.Chem_292_10014
Author(s) : Wu D , Zhao L , Feng Z , Yu C , Ding J , Wang L , Wang F , Liu D , Zhu H , Xing F , Conaway JW , Conaway RC , Cai Y , Jin J
Ref : Journal of Biological Chemistry , 292 :10014 , 2017
PubMedID: 28450392
Gene_locus related to this paper: human-KANSL3

Title : An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis - Meunier_2015_Nat.Commun_6_7889
Author(s) : Meunier S , Shvedunova M , Van Nguyen N , Avila L , Vernos I , Akhtar A
Ref : Nat Commun , 6 :7889 , 2015
PubMedID: 26243146
Gene_locus related to this paper: human-KANSL3

Title : SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans - Yucel_2014_Dev.Biol_392_266
Author(s) : Yucel D , Hoe M , Llamosas E , Kant S , Jamieson C , Young PA , Crossley M , Nicholas HR
Ref : Developmental Biology , 392 :266 , 2014
PubMedID: 24882710
Gene_locus related to this paper: caeel-q22992

Title : Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex - Cai_2010_J.Biol.Chem_285_4268
Author(s) : Cai Y , Jin J , Swanson SK , Cole MD , Choi SH , Florens L , Washburn MP , Conaway JW , Conaway RC
Ref : Journal of Biological Chemistry , 285 :4268 , 2010
PubMedID: 20018852
Gene_locus related to this paper: human-KANSL3