Zaninetti_2000_Neurosci_95_319

Reference

Title : Magnocellular neurons of the rat supraoptic nucleus are endowed with functional nicotinic acetylcholine receptors - Zaninetti_2000_Neurosci_95_319
Author(s) : Zaninetti M , Blanchet C , Tribollet E , Bertrand D , Raggenbass M
Ref : Neuroscience , 95 :319 , 2000
Abstract :

Acetylcholine can stimulate the release of vasopressin. In organ-cultured hypothalamo-neurohypophyseal systems, acetylcholine enhanced vasopressin release by acting in or near the supraoptic nucleus Extracellular recordings suggested that acetylcholine can increase supraoptic neuron excitability. These effects could be mimicked, in part, by nicotine or blocked by nicotinic antagonists, suggesting that they might be mediated by nicotinic acetylcholine receptors. Autoradiography indicated that alpha-bungarotoxin binding sites are present in the supraoptic nucleus; however, neither acetylcholine nor nicotine binding sites could be detected. Thus, the existence, let alone the nature, of nicotinic receptors in the supraoptic nucleus has so far remained elusive. The present work attempts to determine: (i) whether functional nicotinic receptors are present in this nucleus; (ii) whether they are located on neurosecretory magnocellular cells or at presynaptic sites; (iii) what their pharmacological and biophysical properties are; (iv) whether they influence the activity of all or only part of supraoptic neurons. Whole-cell recordings were performed in hypothalamic slices or in acutely dissociated supraoptic neurons and the effect of nicotinic agonists was tested under voltage-clamp conditions. Autoradiography was done in coronal hypothalamic sections, using [3H]epibatidine and [125I]alpha-bungarotoxin as ligands. Our results indicate that supraoptic neurons possess functional nicotinic receptors containing the alpha7 subunit.

PubMedSearch : Zaninetti_2000_Neurosci_95_319
PubMedID: 10658610

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Citations formats

Zaninetti M, Blanchet C, Tribollet E, Bertrand D, Raggenbass M (2000)
Magnocellular neurons of the rat supraoptic nucleus are endowed with functional nicotinic acetylcholine receptors
Neuroscience 95 :319

Zaninetti M, Blanchet C, Tribollet E, Bertrand D, Raggenbass M (2000)
Neuroscience 95 :319