Zeng_2022_ACS.Catal_12_3033

Reference

Title : Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy - Zeng_2022_ACS.Catal_12_3033
Author(s) : Zeng W , Li X , Yang Y , Min J , Huang JW , Liu W , Niu D , Yang X , Han X , Zhang L , Dai L , Chen CC , Guo RT
Ref : ACS Catal , 12 :3033 , 2022
Abstract :

Polyethylene terephthalate (PET) is among the most extensively produced plastics, but huge amounts of PET wastes that have accumulated in the environment have become a serious threat to the ecosystem. Applying PET hydrolytic enzymes to depolymerize PET is an attractive measure to manage PET pollution, and searching for more effective enzymes is a prerequisite to achieve this goal. A thermostable cutinase that originates from the leaf-branch compost termed ICCG is the most effective PET hydrolase reported so far. Here, we illustrated the crystal structure of ICCG in complex with the PET analogue, mono(2-hydroxyethyl)terephthalic acid, to reveal the enzyme-substrate interaction network. Furthermore, we applied structure-based engineering to modify ICCG and screened for variants that exhibit higher efficacy than the parental enzyme. As a result, several variants with the measured melting temperature approaching 99 C and elevated PET hydrolytic activity were obtained. Finally, crystallographic analyses were performed to reveal the structural stabilization effects mediated by the introduced mutations. These results are of importance in the context of understanding the mechanism of action of the thermostable PET hydrolytic enzyme and shall be beneficial to the development of PET biodegradation platforms.

PubMedSearch : Zeng_2022_ACS.Catal_12_3033
PubMedID:
Gene_locus related to this paper: 9bact-g9by57

Related information

Inhibitor MHET
Gene_locus MHET    9bact-g9by57
Structure MHET    9bact-g9by57    7VVC    7VVE    7W1N    7W44    7W45

Citations formats

Zeng W, Li X, Yang Y, Min J, Huang JW, Liu W, Niu D, Yang X, Han X, Zhang L, Dai L, Chen CC, Guo RT (2022)
Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy
ACS Catal 12 :3033

Zeng W, Li X, Yang Y, Min J, Huang JW, Liu W, Niu D, Yang X, Han X, Zhang L, Dai L, Chen CC, Guo RT (2022)
ACS Catal 12 :3033