| Title : PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains - Zhang_2001_FEMS.Microbiol.Lett_198_165 |
| Author(s) : Zhang G , Hang X , Green P , Ho KP , Chen GQ |
| Ref : FEMS Microbiology Letters , 198 :165 , 2001 |
|
Abstract :
Two polyhydroxyalkanoate synthase genes, phaC1 from Pseudomonas pseudoalcaligenes HBQ06 and phaC2 from Pseudomonas nitroreducens 0802, were cloned using a PCR cloning strategy based on the type II pha loci property of Pseudomonas strains. The complete open reading frames (ORFs) of phaC1 (P. nitroreducens HBQ06) and phaC2 (P. nitroreducens 0802) were identified from the PCR products. Using the sequence information, the complete PHA synthase genes were PCR cloned directly from the genomic DNA and expressed in Escherichia coli as confirmed by Fourier transform-infrared spectroscopy and gas chromatography. The differences between PhaC1 and PhaC2 were analyzed and the two proteins were suggested to contain different functions and evolution history. |
| PubMedSearch : Zhang_2001_FEMS.Microbiol.Lett_198_165 |
| PubMedID: 11430409 |
| Gene_locus related to this paper: pseme-PHAC1 , pseme-PHAC2 , pseme-PHAZ , psepf-PHAZ |
| Gene_locus | pseme-PHAC1 pseme-PHAC2 pseme-PHAZ psepf-PHAZ |
Zhang G, Hang X, Green P, Ho KP, Chen GQ (2001)
PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two Pseudomonas strains
FEMS Microbiology Letters
198 :165
Zhang G, Hang X, Green P, Ho KP, Chen GQ (2001)
FEMS Microbiology Letters
198 :165