Title : Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis - Zhang_2014_Nat.Chem.Biol_10_1028 |
Author(s) : Zhang Y , van Dijk AD , Scaffidi A , Flematti GR , Hofmann M , Charnikhova T , Verstappen F , Hepworth J , van der Krol S , Leyser O , Smith SM , Zwanenburg B , Al-Babili S , Ruyter-Spira C , Bouwmeester HJ |
Ref : Nat Chemical Biology , 10 :1028 , 2014 |
Abstract :
Strigolactones (SLs) are a class of phytohormones and rhizosphere signaling compounds with high structural diversity. Three enzymes, carotenoid isomerase DWARF27 and carotenoid cleavage dioxygenases CCD7 and CCD8, were previously shown to convert all-trans-beta-carotene to carlactone (CL), the SL precursor. However, how CL is metabolized to SLs has remained elusive. Here, by reconstituting the SL biosynthetic pathway in Nicotiana benthamiana, we show that a rice homolog of Arabidopsis More Axillary Growth 1 (MAX1), encodes a cytochrome P450 CYP711 subfamily member that acts as a CL oxidase to stereoselectively convert CL into ent-2'-epi-5-deoxystrigol (B-C lactone ring formation), the presumed precursor of rice SLs. A protein encoded by a second rice MAX1 homolog then catalyzes the conversion of ent-2'-epi-5-deoxystrigol to orobanchol. We therefore report that two members of CYP711 enzymes can catalyze two distinct steps in SL biosynthesis, identifying the first enzymes involved in B-C ring closure and a subsequent structural diversification step of SLs. |
PubMedSearch : Zhang_2014_Nat.Chem.Biol_10_1028 |
PubMedID: 25344813 |
Substrate | Carlactone |
Zhang Y, van Dijk AD, Scaffidi A, Flematti GR, Hofmann M, Charnikhova T, Verstappen F, Hepworth J, van der Krol S, Leyser O, Smith SM, Zwanenburg B, Al-Babili S, Ruyter-Spira C, Bouwmeester HJ (2014)
Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis
Nat Chemical Biology
10 :1028
Zhang Y, van Dijk AD, Scaffidi A, Flematti GR, Hofmann M, Charnikhova T, Verstappen F, Hepworth J, van der Krol S, Leyser O, Smith SM, Zwanenburg B, Al-Babili S, Ruyter-Spira C, Bouwmeester HJ (2014)
Nat Chemical Biology
10 :1028