Zhang_2014_Nat.Chem.Biol_10_1028

Reference

Title : Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis - Zhang_2014_Nat.Chem.Biol_10_1028
Author(s) : Zhang Y , van Dijk AD , Scaffidi A , Flematti GR , Hofmann M , Charnikhova T , Verstappen F , Hepworth J , van der Krol S , Leyser O , Smith SM , Zwanenburg B , Al-Babili S , Ruyter-Spira C , Bouwmeester HJ
Ref : Nat Chemical Biology , 10 :1028 , 2014
Abstract : Strigolactones (SLs) are a class of phytohormones and rhizosphere signaling compounds with high structural diversity. Three enzymes, carotenoid isomerase DWARF27 and carotenoid cleavage dioxygenases CCD7 and CCD8, were previously shown to convert all-trans-beta-carotene to carlactone (CL), the SL precursor. However, how CL is metabolized to SLs has remained elusive. Here, by reconstituting the SL biosynthetic pathway in Nicotiana benthamiana, we show that a rice homolog of Arabidopsis More Axillary Growth 1 (MAX1), encodes a cytochrome P450 CYP711 subfamily member that acts as a CL oxidase to stereoselectively convert CL into ent-2'-epi-5-deoxystrigol (B-C lactone ring formation), the presumed precursor of rice SLs. A protein encoded by a second rice MAX1 homolog then catalyzes the conversion of ent-2'-epi-5-deoxystrigol to orobanchol. We therefore report that two members of CYP711 enzymes can catalyze two distinct steps in SL biosynthesis, identifying the first enzymes involved in B-C ring closure and a subsequent structural diversification step of SLs.
ESTHER : Zhang_2014_Nat.Chem.Biol_10_1028
PubMedSearch : Zhang_2014_Nat.Chem.Biol_10_1028
PubMedID: 25344813

Related information

Substrate Carlactone

Citations formats

Zhang Y, van Dijk AD, Scaffidi A, Flematti GR, Hofmann M, Charnikhova T, Verstappen F, Hepworth J, van der Krol S, Leyser O, Smith SM, Zwanenburg B, Al-Babili S, Ruyter-Spira C, Bouwmeester HJ (2014)
Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis
Nat Chemical Biology 10 :1028

Zhang Y, van Dijk AD, Scaffidi A, Flematti GR, Hofmann M, Charnikhova T, Verstappen F, Hepworth J, van der Krol S, Leyser O, Smith SM, Zwanenburg B, Al-Babili S, Ruyter-Spira C, Bouwmeester HJ (2014)
Nat Chemical Biology 10 :1028