| Title : Functional characterization of MpaG', the O-methyltransferase involved in the biosynthesis of mycophenolic acid - Zhang_2015_Chembiochem_16_565 |
| Author(s) : Zhang W , Cao S , Qiu L , Qi F , Li Z , Yang Y , Huang S , Bai F , Liu C , Wan X , Li S |
| Ref : Chembiochem , 16 :565 , 2015 |
|
Abstract :
Mycophenolic acid (MPA, 1) is a clinically important immunosuppressant. In this report, a gene cluster mpa' responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S-adenosyl-L-methionine-dependent (SAM-dependent) O-methyltransferase encoded by the mpaG' gene was functionally and kinetically characterized in vitro. MpaG' catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6) to form 1. It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis. |
| PubMedSearch : Zhang_2015_Chembiochem_16_565 |
| PubMedID: 25630520 |
| Gene_locus related to this paper: penbr-mpaH , penbr-mpac |
| Gene_locus | penbr-mpaH penbr-mpac |
Zhang W, Cao S, Qiu L, Qi F, Li Z, Yang Y, Huang S, Bai F, Liu C, Wan X, Li S (2015)
Functional characterization of MpaG', the O-methyltransferase involved in the biosynthesis of mycophenolic acid
Chembiochem
16 :565
Zhang W, Cao S, Qiu L, Qi F, Li Z, Yang Y, Huang S, Bai F, Liu C, Wan X, Li S (2015)
Chembiochem
16 :565