Zhang_2018_Sci.Rep_8_224

Reference

Title : Multiple mutations and overexpression of the MdaE7 carboxylesterase gene associated with male-linked malathion resistance in housefly, Musca domestica (Diptera: Muscidae) - Zhang_2018_Sci.Rep_8_224
Author(s) : Zhang Y , Li J , Ma Z , Shan C , Gao X
Ref : Sci Rep , 8 :224 , 2018
Abstract : Two unique housefly strains, MSS and N-MRS, were selected and used to clarify mechanisms of sex-associated malathion resistance in the housefly, Musca domestica. Compared with the lab-susceptible CSS strain, susceptible females and resistant males were observed in the malathion-susceptible MSS strain, while the malathion-resistant near-isogenic line, N-MRS, achieved similar resistance level between genders. Significant synergistic effect of the esterase-inhibitor DEF on resistant houseflies pointed to the important involvement of esterase in this specific malathion resistance. Examination of the carboxylesterase gene MdalphaE7 in malathion resistant housefly populations found seven, non-synonymous SNP mutations (Ser(250)-Thr, Trp(251)-Ser, Met(303)-Ile, Leu(354)-Phe, Ser(357)-Leu, Trp(378)-Arg and Ser(383)-Thr), not found in susceptible houseflies, revealing a strong correlation between these mutations and the development of malathion resistance. Further genetic analysis conducted with bioassays by topical application and nucleotide polymorphism detection provided a first line of molecular evidence for a linkage between a male-determining factor and MdalphaE7 gene in the MSS and N-MRS males. This linkage results in a much higher level of malathion resistance for males than females in the MSS strain. Lastly, quantitative real-time PCR showed that MdalphaE7 was over expressed in the resistant strain due to the increased transcription level of mRNA rather than gene duplication.
ESTHER : Zhang_2018_Sci.Rep_8_224
PubMedSearch : Zhang_2018_Sci.Rep_8_224
PubMedID: 29317643
Gene_locus related to this paper: musdo-EST23aes07

Related information

Gene_locus related to this paper: musdo-EST23aes07

Citations formats

Zhang Y, Li J, Ma Z, Shan C, Gao X (2018)
Multiple mutations and overexpression of the MdaE7 carboxylesterase gene associated with male-linked malathion resistance in housefly, Musca domestica (Diptera: Muscidae)
Sci Rep 8 :224

Zhang Y, Li J, Ma Z, Shan C, Gao X (2018)
Sci Rep 8 :224