Title : Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae) - Zhang_2020_Ecotoxicology_29_35 |
Author(s) : Zhang Y , Guo M , Ma Z , You C , Gao X , Shi X |
Ref : Ecotoxicology , 29 :35 , 2020 |
Abstract :
Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdalphaE7 in the two strains revealed that four non-synonymous mutations (Trp(251)-Leu, Asp(273)-Glu, Ala(365)-Val, and Ile(396)-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdalphaE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies. |
PubMedSearch : Zhang_2020_Ecotoxicology_29_35 |
PubMedID: 31749037 |
Gene_locus related to this paper: musdo-EST23aes07 |
Zhang Y, Guo M, Ma Z, You C, Gao X, Shi X (2020)
Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae)
Ecotoxicology
29 :35
Zhang Y, Guo M, Ma Z, You C, Gao X, Shi X (2020)
Ecotoxicology
29 :35