Title : Constructing Cross-linked Nanofibrous Scaffold via Dual-Enzyme-Instructed Hierarchical Assembly - Zhang_2020_Langmuir__ |
Author(s) : Zhang S , Cortes W , Zhang Y |
Ref : Langmuir , : , 2020 |
Abstract :
To explore the potential of step-by-step assembly in the fabrication of biological materials, we designed and synthesized two peptide-based molecules for enzyme-instructed hierarchical assembly (EIHA). Upon the treatment of alkaline phosphatase (ALP), one molecule undergoes enzyme-instructed self-assembly (EISA) forming uniformed nanofibers. The other one that can self-assemble into vesicles undergoes enzyme-induced transformation of self-assembly (EITSA) converting vesicles into irregular aggregates upon the treatment of carboxylesterase (CES). Co-administration of two enzymes to a mixture of these two molecules in a stage-by-stage fashion leads to a physically knotted nanofibrous scaffold that is applicable as a nanostructured matrix for cell culture. |
PubMedSearch : Zhang_2020_Langmuir__ |
PubMedID: 32418429 |
Zhang S, Cortes W, Zhang Y (2020)
Constructing Cross-linked Nanofibrous Scaffold via Dual-Enzyme-Instructed Hierarchical Assembly
Langmuir
:
Zhang S, Cortes W, Zhang Y (2020)
Langmuir
: