Zhang_2025_Food.Chem_481_144017

Since early 1990s, diacylglycerol (DAG) has drawn a continuous trending interest among researchers and oil industries/markets as part of a reduced-energy diet due to its functions to prevent and manage obesity. With the accumulated knowledge, a stereoisomer of sn-1,3-DAG is regarded as the sole compound to contribute to DAG's functions. sn-1,3-DAG can be produced by direct esterification of free fatty acids and glycerol, partial hydrolysis of TAGs/edible oils, and glycerolysis of TAGs/edible oils with glycerol using the regioselective microbial lipases as the catalyst. However, the specific microbial lipases with high efficiency to produce sn-1,3-DAG and their catalytic mechanisms are still a mystery. Herein, we provide an overview of metabolic fates of three stereoisomers of DAGs including sn-1,3-DAG, sn-1,2-DAG and/or sn-2,3-DAG, and synthesis process for sn-1,3-DAG, and critically outline the microbial lipases to selectively produce sn-1,3-DAG, and their pathways and mechanisms, which hopefully presents a reasonable full picture of functions, synthesis schemes, and catalytic performance to improve regioselectivity and catalytic efficiency for sn-1,3-DAG production with high yield.