Cui F

References (14)

Title : Novel Dual-Functional Enzyme Lip10 Catalyzes Lipase and Acyltransferase Activities in the Oleaginous Fungus Mucor circinelloides - Zan_2019_J.Agric.Food.Chem_67_13176
Author(s) : Zan X , Cui F , Sun J , Zhou S , Song Y
Ref : Journal of Agricultural and Food Chemistry , 67 :13176 , 2019
Abstract : Lipases or triacylglycerol (TAG) lipases belong to the alpha/beta-hydrolases superfamily, which are enzymes capable of catalyzing the hydrolysis of the ester bond between fatty acids and glycerol. Interestingly, some lipases have been found to not only possess hydrolysis activity but also acyltransferase activity in yeasts and microalgae. Our present study reported a novel dual-functional Mucor circinelloides lipase Lip10 with a slight lipolysis activity but a noteworthy phospholipid/diacylglycerol acyltransferase (PDAT) activity. The purified Lip10 mutants prefer to utilize phosphatidyl serine to form TAG over phosphatidyl ethanolamine and phosphatidylcholine. Site-directed mutagenesis indicated that the histidine residue in the acyltransferase motif H-(X)4-D is indispensable for the PDAT activity of Lip10. Overexpression of the acyltransferase motif of Lip10 promoted cell growth by 12% and increased lipid production by 14% compared to the control, whilst overexpression of the lipase motif induced lipid degradation in M. circinelloides.
ESTHER : Zan_2019_J.Agric.Food.Chem_67_13176
PubMedSearch : Zan_2019_J.Agric.Food.Chem_67_13176
PubMedID: 31690075
Gene_locus related to this paper: muccl-a0a168kyh0 , muccl-a0a168mc90 , muccl-a0a168nw20

Title : Carboxylesterase-mediated insecticide resistance: Quantitative increase induces broader metabolic resistance than qualitative change - Cui_2015_Pestic.Biochem.Physiol_121_88
Author(s) : Cui F , Li MX , Chang HJ , Mao Y , Zhang HY , Lu LX , Yan SG , Lang ML , Liu L , Qiao CL
Ref : Pestic Biochem Physiol , 121 :88 , 2015
Abstract : Carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (OP) insecticides. Carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (G/A151D) or from tryptophan to leucine at the 271 site (W271L), following the numbering of Drosophila melanogaster AChE. Qualitative mechanism has been observed in few species. However, whether this carboxylesterase mutation mechanism is prevalent in insects remains unclear. In this study, wild-type, G/A151D and W271L mutant carboxylesterases from Culex pipiens and Aphis gossypii were subjected to germline transformation and then transferred to D. melanogaster. These germlines were ubiquitously expressed as induced by tub-Gal4. In carboxylesterase activity assay, the introduced mutant carboxylesterase did not enhance the overall carboxylesterase activity of flies. This result indicated that G/A151D or W271L mutation disrupted the original activities of the enzyme. Less than 1.5-fold OP resistance was only observed in flies expressing A. gossypii mutant carboxylesterases compared with those expressing A. gossypii wild-type carboxylesterase. However, transgenic flies universally showed low resistance to OP insecticides compared with non-transgenic flies. The flies expressing A. gossypii W271L mutant esterase exhibited 1.5-fold resistance to deltamethrin, a pyrethroid insecticide compared with non-transgenic flies. The present transgenic Drosophila system potentially showed that a quantitative increase in carboxylesterases induced broader resistance of insects to insecticides than a qualitative change.
ESTHER : Cui_2015_Pestic.Biochem.Physiol_121_88
PubMedSearch : Cui_2015_Pestic.Biochem.Physiol_121_88
PubMedID: 26047115

Title : Over-transcription of genes in a parathion-resistant strain of mosquito Culex pipiens quinquefasciatus - Wang_2015_Insect.Sci_22_150
Author(s) : Wang W , Liu SL , Liu YY , Qiao CL , Chen SL , Cui F
Ref : Insect Sci , 22 :150 , 2015
Abstract : Insecticide resistance is an evolutionary adaptation that develops quite quickly in mosquitoes because of the high selection pressure of chemical insecticides, rapid generation time and large population size. Identification of genes associated with insecticide resistance is fundamental to understand the complex processes responsible for resistance. We compared the gene transcriptional profiles of parathion-resistant and -susceptible Culex pipiens quinquefasciatus using a combination of suppression subtractive hybridization and complementary DNA (cDNA) microarray techniques. A total of 278 colonies were selected from the resistant-susceptible mosquito subtractive library, 38 of which showed more than two fold stronger immunoblotting signals in the resistant strain than in the susceptible strain using cDNA microarray selection. The sequencing results showed that the 38 colonies can be matched to 12 genes of C. p. quinquefasciatus. Eight genes were confirmed to be overexpressed by more than two fold in the resistant strain. These genes encode chymotrypsin-1, theta glutathione S-transferase, lipase 3, larval serum protein 1 beta chain, cytochrome b, mitochondrial ribosomal large subunit, 28S rRNA, and a protein with unknown function. This study serves as a preliminary attempt to identify new genes associated with organophosphate resistance in this mosquito species and provides insights into the complicated physiological phenomenon of insecticide resistance.
ESTHER : Wang_2015_Insect.Sci_22_150
PubMedSearch : Wang_2015_Insect.Sci_22_150
PubMedID: 24431295

Title : High-throughput genotyping of single-nucleotide polymorphisms in ace-1 gene of mosquitoes using MALDI-TOF mass spectrometry - Mao_2013_Insect.Sci_20_167
Author(s) : Mao Y , Tan F , Yan SG , Wu GX , Qiao CL , Zhang WX , Cui F
Ref : Insect Sci , 20 :167 , 2013
Abstract : Abstract Acetylcholinesterase (AChE) plays a vital role in the nervous system of insects and other animal species and serves as the target for many chemical agents such as organophosphate and carbamate insecticides. The mosquito, Culex pipiens complex, a vector of human disease, has evolved to be resistant to insecticides by a limited number of amino acid substitutions in AChE1, which is encoded by the ace-1 gene. The aims of this study are to identify single nucleotide polymorphism (SNP) sites in the ace-1 gene of the C. pipiens complex and explore an economical high-throughput method to differentiate the genotypes of these sites in mosquitoes collected in the field. We identified 22 SNP sites in exon regions of the ace-1 gene. Four of them led to non-synonymous mutations, that is, Y163C, G247S, C677S and T682A. We used matrix-assisted laser desorption ionization - time-of-flight mass spectrometry for genotyping at these four sites and another site F416V, which was relevant to insecticide resistance, in 150 mosquitoes collected from 15 field populations. We were able to synchronize analysis of the five SNP sites in each well of a 384-well plate for each individual mosquito, thus decreasing the cost to one-fifth of the routine analysis. Heterozygous genotypes at Y163C and G247S sites were observed in one mosquito. The possible influence of the five SNP sites on the activity or function of the enzyme is discussed based on the predicted tertiary structure of the enzyme.
ESTHER : Mao_2013_Insect.Sci_20_167
PubMedSearch : Mao_2013_Insect.Sci_20_167
PubMedID: 23955857

Title : Transcriptomic and phylogenetic analysis of Culex pipiens quinquefasciatus for three detoxification gene families - Yan_2012_BMC.Genomics_13_609
Author(s) : Yan L , Yang P , Jiang F , Cui N , Ma E , Qiao C , Cui F
Ref : BMC Genomics , 13 :609 , 2012
Abstract : ABSTRACT BACKGROUND The genomes of three major mosquito vectors of human diseases Anopheles gambiae Aedes aegypti and Culex pipiens quinquefasciatus have been previously sequenced C p quinquefasciatus has the largest number of predicted protein-coding genes which partially results from the expansion of three detoxification gene families cytochrome P450 monooxygenases P450 glutathione S-transferases GST and carboxyl/cholinesterases CCE However unlike An gambiae and Ae aegypti which have large amounts of gene expression data C p quinquefasciatus has limited transcriptomic resources Knowledge of complete gene expression information is very important for the exploration of the functions of genes involved in specific biological processes In the present study the three detoxification gene families of C p quinquefasciatus were analyzed for phylogenetic classification and compared with those of three other dipteran insects Gene expression during various developmental stages and the differential expression responsible for parathion resistance were profiled using the digital gene expression DGE technique RESULTS A total of 302 detoxification genes were found in C p quinquefasciatus including 71 CCE 196 P450 and 35 cytosolic GST genes Compared with three other dipteran species gene expansion in Culex mainly occurred in the CCE and P450 families where the genes of alpha-esterases juvenile hormone esterases and CYP325 of the CYP4 subfamily showed the most pronounced expansion on the genome For the five DGE libraries 3.5-3.8 million raw tags were generated and mapped to 13314 reference genes Among 302 detoxification genes 225 75 were detected for expression in at least one DGE library One fourth of the CCE and P450 genes were detected uniquely in one stage indicating potential developmentally regulated expression A total of 1511 genes showed different expression levels between a parathion-resistant and a susceptible strain Fifteen detoxification genes including 2 CCEs 6 GSTs and 7 P450s were expressed at higher levels in the resistant strain CONCLUSIONS The results of the present study provide new insights into the functions and evolution of three detoxification gene families in mosquitoes and comprehensive transcriptomic resources for C p quinquefasciatus which will facilitate the elucidation of molecular mechanisms underlying the different biological characteristics of the three major mosquito vectors.
ESTHER : Yan_2012_BMC.Genomics_13_609
PubMedSearch : Yan_2012_BMC.Genomics_13_609
PubMedID: 23140097

Title : Identification of resistant carboxylesterase alleles in Culex pipiens complex via PCR-RFLP - Zhang_2012_Parasit.Vectors_5_209
Author(s) : Zhang H , Meng F , Qiao C , Cui F
Ref : Parasit Vectors , 5 :209 , 2012
Abstract : BACKGROUND: Carboxylesterase overproduction is a frequently observed resistance mechanism of insects to organophosphate insecticides. As a major transmitter of human diseases, mosquitoes in the Culex pipiens complex have evolved 13 carboxylesterase alleles (Ester) that confer organophosphate resistance. Six alleles, Ester(B1), Ester(2), Ester(8), Ester(9), Ester(B10), and Ester(1)(1), have been observed in field populations in China, sometimes co-existing in one population. To differentiate the carboxylesterase alleles found in these field populations, PCR-RFLP was designed for use in resistance monitoring. RESULTS: Based on the DNA sequences of resistant and nonresistant carboxylesterase alleles, Ester B alleles were first amplified with PCR-specific primers and then digested with the restriction enzyme DraI. In this step, Ester(2) and Ester(1)(1) were differentiated from the other Ester alleles. When the other Ester B alleles were digested with the restriction enzyme XbaI, Ester(B1) and the susceptible C. p. pallens Ester were screened out. Ester(8) and Ester(9) were differentiated from Ester(B10) and the susceptible C. p. quinquefasciatus esterase allele, respectively, by amplifying and digesting the Ester A alleles with the restriction enzyme ApaLI. The effectiveness of the custom-designed PCR-RFLP was verified in two field mosquito populations. CONCLUSIONS: A PCR-RFLP based approach was developed to differentiate carboxylesterase alleles in Culex pipiens complex mosquitoes. These processes may be useful in monitoring the evolutionary dynamics of known carboxylesterase alleles as well as in the identification of new alleles in field populations.
ESTHER : Zhang_2012_Parasit.Vectors_5_209
PubMedSearch : Zhang_2012_Parasit.Vectors_5_209
PubMedID: 23006470
Gene_locus related to this paper: culpi-ESTA

Title : Correlation between carboxylesterase alleles and insecticide resistance in Culex pipiens complex from China - Liu_2011_Parasit.Vectors_4_236
Author(s) : Liu Y , Zhang H , Qiao C , Lu X , Cui F
Ref : Parasit Vectors , 4 :236 , 2011
Abstract : BACKGROUND: In China, large amounts of chemical insecticides are applied in fields or indoors every year, directly or indirectly bringing selection pressure on vector mosquitoes. Culex pipiens complex has evolved to be resistant to all types of chemical insecticides, especially organophosphates, through carboxylesterases. Six resistant carboxylesterase alleles (Ester) were recorded previously and sometimes co-existed in one field population, representing a complex situation for the evolution of Ester genes. RESULTS: In order to explore the evolutionary scenario, we analyzed the data from an historical record in 2003 and a recent investigation on five Culex pipiens pallens populations sampled from north China in 2010. Insecticide bioassays showed that these five populations had high resistance to pyrethroids, medium resistance to organophosphates, and low resistance to carbamates. Six types of Ester alleles, EsterB1, Ester2, Ester8, Ester9, EsterB10, and Ester11 were identified, and the overall pattern of their frequencies in geographic distribution was consistent with the report seven years prior to this study. Statistical correlation analysis indicated that Ester8 and Ester9 positively correlated with resistance to four insecticides, and EsterB10 to one insecticide. The occurrences of these three alleles were positively correlated, while the occurrence of EsterB1 was negatively correlated with Ester8, indicating an allelic competition. CONCLUSION: Our analysis suggests that one insecticide can select multiple Ester alleles and one Ester allele can work on multiple insecticides. The evolutionary scenario of carboxylesterases under insecticide selection is possibly "one to many".
ESTHER : Liu_2011_Parasit.Vectors_4_236
PubMedSearch : Liu_2011_Parasit.Vectors_4_236
PubMedID: 22177233

Title : Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects - Cui_2011_Insect.Biochem.Mol.Biol_41_1
Author(s) : Cui F , Lin Z , Wang H , Liu S , Chang H , Reeck G , Qiao C , Raymond M , Kang L
Ref : Insect Biochemistry & Molecular Biology , 41 :1 , 2011
Abstract : Carboxylesterases provide key mechanisms of resistance to insecticides, particularly organophosphates (OPs), in insects. One resistance mechanism is a qualitative change in the properties of a carboxylesterase. Two mutant forms, G151D and W271L, have been observed, mostly in dipteran species, to affect substrate specificity of enzymes. But whether these two single mutations can commonly change character of insect carboxylesterases is unknown. In our study carboxylesterase genes from seven insects distributed among four orders were cloned, mutated at position 151 or 271 and expressed in Escherichia coli. The kinetics of the purified recombinant proteins was examined towards an artificial carboxylester and two OP insecticides. The G/A151D and W271L mutation significantly reduced carboxylesterase activity in 87.5% and 100% cases, respectively, and at the same time conferred OP hydrolase activities in 62.5% and 87.5% cases, respectively. Thus, the change at position 271 is more effective to influence substrate specificity than that at position 151. These results may suggest that these two mutations have the potential to cause insecticide resistance broadly in insects.
ESTHER : Cui_2011_Insect.Biochem.Mol.Biol_41_1
PubMedSearch : Cui_2011_Insect.Biochem.Mol.Biol_41_1
PubMedID: 20888910
Gene_locus related to this paper: aphgo-cxest

Title : Dynamics of esterase alleles in Culex pipiens complex mosquitoes in Beijing - Yan_2008_J.Econ.Entomol_101_1897
Author(s) : Yan S , Wu Z , Cui F , Zhao Q , Qiao C
Ref : J Econ Entomol , 101 :1897 , 2008
Abstract : To investigate insecticide resistance levels and dynamic changes of carboxylesterase polymorphism with time in resistant populations of mosquitoes in the Culex pipiens complex, four field populations were collected in September 2006. The resistance levels of fourth-instar larvae to organophosphate (dichlorvos, parathion, and chlorpyrifos), carbamate (fenobucarb and propoxur), and pyrethroid (permethrin and tetramethrin) insecticides were determined by bioassay. Larvae had a low but significant resistance to organophosphate and carbamate insecticides but no significant resistance to pyrethroid insecticides. Starch gel electrophoresis revealed the presence of the overproduced esterases B1, A2-B2, A8-B8, A9-B9, and All-B11. The frequency of each overproduced esterases varied depending on its regional localities. Compared with previous surveys, the polymorphism of amplified esterase alleles in Beijing populations increased, although the resistance level to organophosphate insecticides declined.
ESTHER : Yan_2008_J.Econ.Entomol_101_1897
PubMedSearch : Yan_2008_J.Econ.Entomol_101_1897
PubMedID: 19133472

Title : Filariasis vector in China: insecticide resistance and population structure of mosquito Culex pipiens complex - Cui_2007_Pest.Manag.Sci_63_453
Author(s) : Cui F , Tan Y , Qiao C-L
Ref : Pest Manag Sci , 63 :453 , 2007
Abstract : Seven field populations of mosquito Culex pipiens complex (Diptera: Culicidae) were collected from four provinces of China. The resistance status of larvae to dichlorvos, parathion, chlorpyrifos, fenobucarb (BPMC) and propoxur were determined by bioassays, disclosing that they were more resistant to organophosphate (moderate or low resistance) than to carbamate (low or no significant resistance) insecticides. Starch gel electrophoresis confirmed the presence and distribution of overproduced esterases B1, A2-B2, A8-B8 and A9-B9, the frequencies of which varied according to their regional origins. Electrophoretic polymorphism at four putatively neutral loci (got-1, got-2, pgi and pgm) showed that the overall genetic differentiation found across all populations was significantly large (Fst = 0.28, P < 10(-4)), and genetic exchange was slightly restricted by distance isolation (P = 0.018).
ESTHER : Cui_2007_Pest.Manag.Sci_63_453
PubMedSearch : Cui_2007_Pest.Manag.Sci_63_453
PubMedID: 17387711

Title : Different amino-acid substitutions confer insecticide resistance through acetylcholinesterase 1 insensitivity in Culex vishnui and Culex tritaeniorhynchus (Diptera: Culicidae) from China - Alout_2007_J.Med.Entomol_44_463
Author(s) : Alout H , Berthomieu A , Cui F , Tan Y , Berticat C , Qiao C , Weill M
Ref : Journal of Medical Entomology , 44 :463 , 2007
Abstract : Insecticide resistance owing to insensitive acetylcholinesterase (AChE)1 has been reported in several mosquito species, and only two mutations in the ace-1 gene have been implicated in resistance: 119S and 331W substitutions. We analyzed the AChE1 resistance status of Culex vishnui (Theobald) and Culex tritaeniorhynchus Giles sampled in various regions of China. These two species displayed distinct mutations leading to AChE1 insensitivity; the 119S substitution in resistant C. vishnui mosquitoes and the 331W substitution in resistant C. tritaeniorhynchus. A biochemical test was validated to detect the 331W mutation in field samples. The comparison of the recombinant G119S and 331W mutant proteins produced in vitro with the AChE1 extracted from resistant mosquitoes indicated that the AChE1 insensitivity observed could be specifically attributed to these substitutions. Comparison of their biochemical characteristics indicated that the resistance conferred by these mutations depends on the insecticide used, regardless of its class. This resistance seemed to be fixed in the Cx. tritaeniorhynchus populations sampled in a 2000-km transect, suggesting a very high level of insecticide application or a low fitness cost associated with this 331W mutation.
ESTHER : Alout_2007_J.Med.Entomol_44_463
PubMedSearch : Alout_2007_J.Med.Entomol_44_463
PubMedID: 17547232
Gene_locus related to this paper: culpi-ACHE1

Title : Characterization of novel esterases in insecticide-resistant mosquitoes - Cui_2007_Insect.Biochem.Mol.Biol_37_1131
Author(s) : Cui F , Weill M , Berthomieu A , Raymond M , Qiao CL
Ref : Insect Biochemistry & Molecular Biology , 37 :1131 , 2007
Abstract : In the mosquito Culex pipiens complex (Diptera: Culicidae), the amplification of carboxylesterase genes is an important mechanism providing resistance to organophosphate insecticides. Various amplified alleles at the Ester locus have been identified over the world. In this study, two newly detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively. A high molecular identity is observed both at the nucleotide level and at the deduced amino acid level among the known Ester alleles. Real-time quantitative PCR results suggest 2.5-fold amplification of the Ester(B10) allele, 36.5-fold amplification of the Ester(A11) allele, and 19.1-fold amplification of the Ester(B11) allele. The ca. 2-fold difference in amplification level between Ester(A11) and Ester(B11) may indicate a new model for the esterase amplification. Bioassays show that these two resistant Ester alleles only can confer moderate or low resistance to the tested organophosphate insecticides.
ESTHER : Cui_2007_Insect.Biochem.Mol.Biol_37_1131
PubMedSearch : Cui_2007_Insect.Biochem.Mol.Biol_37_1131
PubMedID: 17916499
Gene_locus related to this paper: culpi-ESTA , culqu-1estb

Title : Genetic differentiation of Culex pipiens (Diptera: Culicidae) in China - Cui_2007_Bull.Entomol.Res_97_291
Author(s) : Cui F , Qiao CL , Shen BC , Marquine M , Weill M , Raymond M
Ref : Bull Entomol Res , 97 :291 , 2007
Abstract : The population genetic structures of Culex pipiens Linnaeus were evaluated in China over a 2000 km transect that encompasses the two subspecies, C. p. pallens and C. p. quinquefasciatus. Four polymorphic allozyme loci were investigated in 1376 mosquitoes sampled from 20 populations across four provinces. These loci were not statistically dependent with no apparent heterozygote deficit or excess. On a regional scale (intra-province), a low (Fst=0.007-0.016) and significant genetic differentiation was found, with no clear geographical pattern. On a wider scale (inter-province), the genetic differentiation was higher (Fst=0.059), and an isolation by distance emerged. The results are compared with previous population genetic surveys of this mosquito species in different geographic areas over the world. The overall pattern suggests that Culex pipiens requires considerable distance (500-1000 km) to show isolation by distance, irrespective of the subspecies (C. p. pipiens, C. p. quinquefasciatus and C. p. pallens) or the geographic location.
ESTHER : Cui_2007_Bull.Entomol.Res_97_291
PubMedSearch : Cui_2007_Bull.Entomol.Res_97_291
PubMedID: 17524160

Title : Recent emergence of insensitive acetylcholinesterase in Chinese populations of the mosquito Culex pipiens (Diptera: Culicidae) - Cui_2006_J.Med.Entomol_43_878
Author(s) : Cui F , Raymond M , Berthomieu A , Alout H , Weill M , Qiao CL
Ref : Journal of Medical Entomology , 43 :878 , 2006
Abstract : Organophosphate/carbamate target resistance has emerged in Culex pipiens L. (Diptera: Culicidae), the vector of Wuchereria bancrofti and West Nile virus (family Flaviviridae, genus Flavivirus) in China. The insensitive acetylcholinesterase was detected in only one of 20 samples collected on a north-to-south transect. According to previous findings, a unique mutation, G119S in the ace-1 gene, explained this high insensitivity. Phylogenetic analysis indicates that the mutation G119S recently detected in China results from an independent mutation event. The G119S mutation thus occurred at least three times independently within the Cx. pipiens complex, once in the temperate (Cx. p. pipiens) and twice in the tropical form (Cx. p. quinquefasciatus). Bioassays performed with a purified G119S strain indicated that this substitution was associated with high levels of resistance to chlorpyrifos, fenitrothion, malathion, and parathion, but low levels of resistance to dichlorvos, trichlorfon, and fenthion. Hence, it is possible that in China, dichlorvos, trichlorfon, and fenthion will still achieve effective control even in the presence of the G119S mutation.
ESTHER : Cui_2006_J.Med.Entomol_43_878
PubMedSearch : Cui_2006_J.Med.Entomol_43_878
PubMedID: 17017223
Gene_locus related to this paper: culpi-ACHE1