Title : LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer - Zhao_2023_J.Immunother.Cancer_11_ |
Author(s) : Zhao T , Liu S , Hanna NH , Jalal S , Ding X , Wan J , Yan C , Du H |
Ref : J Immunother Cancer , 11 : , 2023 |
Abstract :
BACKGROUND: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of cells in tumor microenvironment, which suppress antitumor immunity. Expansion of various MDSC subpopulations is closely associated with poor clinical outcomes in cancer. Lysosomal acid lipase (LAL) is a key enzyme in the metabolic pathway of neutral lipids, whose deficiency (LAL-D) in mice induces the differentiation of myeloid lineage cells into MDSCs. These Lal (-/-) MDSCs not only suppress immune surveillance but also stimulate cancer cell proliferation and invasion. Understanding and elucidating the underlying mechanisms of MDSCs biogenesis will help to facilitate diagnosis/prognosis of cancer occurrence and prevent cancer growth and spreading. METHODS: Single-cell RNA sequencing (scRNA-seq) was performed to distinguish intrinsic molecular and cellular differences between normal versus Lal (-/-) bone marrow-derived Ly6G(+) myeloid populations in mice. In humans, LAL expression and metabolic pathways in various myeloid subsets of blood samples of patients with non-small cell lung cancer (NSCLC) were assessed by flow cytometry. The profiles of myeloid subsets were compared in patients with NSCLC before and after the treatment of programmed death-1 (PD-1) immunotherapy. RESULTS: scRNA-seq of Lal (-/-) CD11b(+)Ly6G(+) MDSCs identified two distinctive clusters with differential gene expression patterns and revealed a major metabolic shift towards glucose utilization and reactive oxygen species (ROS) overproduction. Blocking pyruvate dehydrogenase (PDH) in glycolysis reversed Lal (-/-) MDSCs' capabilities of immunosuppression and tumor growth stimulation and reduced ROS overproduction. In the blood samples of human patients with NSCLC, LAL expression was significantly decreased in CD13(+)/CD14(+)/CD15(+)/CD33(+) myeloid cell subsets. Further analysis in the blood of patients with NSCLC revealed an expansion of CD13(+)/CD14(+)/CD15(+) myeloid cell subsets, accompanied by upregulation of glucose-related and glutamine-related metabolic enzymes. Pharmacological inhibition of the LAL activity in the blood cells of healthy participants increased the numbers of CD13(+) and CD14(+) myeloid cell subsets. PD-1 checkpoint inhibitor treatment in patients with NSCLC reversed the increased number of CD13(+) and CD14(+) myeloid cell subsets and PDH levels in CD13(+) myeloid cells. CONCLUSION: These results demonstrate that LAL and the associated expansion of MDSCs could serve as targets and biomarkers for anticancer immunotherapy in humans. |
PubMedSearch : Zhao_2023_J.Immunother.Cancer_11_ |
PubMedID: 36914206 |
Gene_locus related to this paper: mouse-1llip |
Gene_locus | mouse-1llip |
Zhao T, Liu S, Hanna NH, Jalal S, Ding X, Wan J, Yan C, Du H (2023)
LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer
J Immunother Cancer
11 :
Zhao T, Liu S, Hanna NH, Jalal S, Ding X, Wan J, Yan C, Du H (2023)
J Immunother Cancer
11 :