Title : An OliGreen-responsive fluorescence sensor for sensitive detection of organophosphorus pesticide based on its specific selectivity towards T-Hg(2+)-T DNA structure - Zhou_2020_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_247_119155 |
Author(s) : Zhou X , Wang C , Wu L , Wei W , Liu S |
Ref : Spectrochim Acta A Mol Biomol Spectrosc , 247 :119155 , 2020 |
Abstract :
In this paper, it was found that OliGreen emitted much stronger fluorescence in rigid T-Hg(2+)-T DNA structure than that in the presence of poly T. Thus, an OliGreen-responsive label-free fluorescent sensor was proposed for sensitive detection of organophosphorus pesticides (OPs) by constructing T-Hg(2+)-T DNA structure. OliGreen emits strong fluorescence in T-Hg(2+)-T structures. The rigid DNA structure of T-Hg(2+)-T is prone to be destroyed by thiocholine (TCh) that hydrolyzed by acetylcholinesterase (AChE) because of the high affinity of TCh with Hg(2+). As a result, T-Hg(2+)-T DNA structure broke down and the fluorescence intensity of OliGreen decreased greatly. With the inhibition of AChE by OPs, fluorescence intensity of OliGreen remained strong because of the rigid T-Hg(2+)-T DNA structure. Thus, a "turn-on" fluorescent sensor which avoids synthesis of nanomaterials and complex label procedures is proposed based on the fluorescence intensity of OliGreen. DDVP were detected with a wide linear range from 0.005 to 25.0 ng/mL and the detection limit was 2.9 pg/mL, which is more sensitive than previously reported methods. |
PubMedSearch : Zhou_2020_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_247_119155 |
PubMedID: 33186818 |
Zhou X, Wang C, Wu L, Wei W, Liu S (2020)
An OliGreen-responsive fluorescence sensor for sensitive detection of organophosphorus pesticide based on its specific selectivity towards T-Hg(2+)-T DNA structure
Spectrochim Acta A Mol Biomol Spectrosc
247 :119155
Zhou X, Wang C, Wu L, Wei W, Liu S (2020)
Spectrochim Acta A Mol Biomol Spectrosc
247 :119155