Zhu_2025_Int.J.Syst.Evol.Microbiol_75_

A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3(T), was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 degreesC (optimum: 30 degreesC) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3(T) could degrade 59.9% of 75.0 microM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3(T); it exhibited low similarity (<31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3(T) was most closely related to strain Comamonas odontotermitis Dant 3-8(T). The 16S rRNA gene similarity between strain J-3(T) and C. odontotermitis Dant 3-8(T) was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3(T) and strain C. odontotermitis Dant 3-8(T) were 81.7% and 25.4%, respectively. The major fatty acids were C(16:0), summed feature 3 (C(16:1) omega7c and/or C(16:1) omega6c) and summed feature 8 (C(18 : 1) omega7c and/or C(18 : 1) omega6c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3(T) was considered to represent a novel species within the genus Comamonas, for which the name Comamonas trifloxystrobinivorans sp. nov. is proposed with strain J-3(T) (=KCTC 8781(T)=MCCC 1K09559(T)) as the type strain.