Zhu_2025_J.Agric.Food.Chem_73_4740

The immobilized kappa-carrageenase MCNF was developed by synergistic integration of kappa-carrageenase, calcium phosphate crystals, and magnetic nanoparticles. MCNF outperformed free kappa-carrageenase in terms of optimal temperature, thermostability (45-55 degreesC), stability (pH 6.0 and 12.0), and storage stability. Furthermore, MCNF was extremely reusable and easy to separate from the reaction system. MCNF's enzymatic breakdown of kappa-carrageenan resulted in a tetrasaccharide that competitively inhibited pancreatic lipase (PL). Fluorescence titration experiments showed that kappa-carrageenan tetrasaccharide altered the microenvironment of PL by causing static bursting of its intrinsic fluorescence. The circular dichroism experiment demonstrated that adding kappa-carrageenan tetrasaccharide reduced alpha-helix content and increased beta-sheet content in PL's secondary structure. Molecular docking and dynamics simulation research suggested that the kappa-carrageenan tetrasaccharide could form a stable complex with PL, entering its hydrophobic cavity and occupying its active site, reducing PL's catalytic activity. kappa-Carrageenan tetrasaccharide could inhibit PL, making it a promising therapeutic agent for obesity prevention and treatment.