de Groot_2009_PLoS.Genet_5_e1000434

Reference

Title : Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti - de Groot_2009_PLoS.Genet_5_e1000434
Author(s) : de Groot A , Dulermo R , Ortet P , Blanchard L , Guerin P , Fernandez B , Vacherie B , Dossat C , Jolivet E , Siguier P , Chandler M , Barakat M , Dedieu A , Barbe V , Heulin T , Sommer S , Achouak W , Armengaud J
Ref : PLoS Genet , 5 :e1000434 , 2009
Abstract :

To better understand adaptation to harsh conditions encountered in hot arid deserts, we report the first complete genome sequence and proteome analysis of a bacterium, Deinococcus deserti VCD115, isolated from Sahara surface sand. Its genome consists of a 2.8-Mb chromosome and three large plasmids of 324 kb, 314 kb, and 396 kb. Accurate primary genome annotation of its 3,455 genes was guided by extensive proteome shotgun analysis. From the large corpus of MS/MS spectra recorded, 1,348 proteins were uncovered and semiquantified by spectral counting. Among the highly detected proteins are several orphans and Deinococcus-specific proteins of unknown function. The alliance of proteomics and genomics high-throughput techniques allowed identification of 15 unpredicted genes and, surprisingly, reversal of incorrectly predicted orientation of 11 genes. Reversal of orientation of two Deinococcus-specific radiation-induced genes, ddrC and ddrH, and identification in D. deserti of supplementary genes involved in manganese import extend our knowledge of the radiotolerance toolbox of Deinococcaceae. Additional genes involved in nutrient import and in DNA repair (i.e., two extra recA, three translesion DNA polymerases, a photolyase) were also identified and found to be expressed under standard growth conditions, and, for these DNA repair genes, after exposure of the cells to UV. The supplementary nutrient import and DNA repair genes are likely important for survival and adaptation of D. deserti to its nutrient-poor, dry, and UV-exposed extreme environment.

PubMedSearch : de Groot_2009_PLoS.Genet_5_e1000434
PubMedID: 19370165
Gene_locus related to this paper: deidv-c1cva5 , deidv-c1cvi6 , deidv-c1cwe6 , deidv-c1cwk6 , deidv-c1cxm7 , deidv-c1cy72 , deidv-c1d002 , deidv-c1d2d8 , deidv-c1d3d8 , deidv-c1d3p0 , deidv-c1cxw0 , deidv-c1d2x1 , deidv-c1cvt7

Related information

Gene_locus deidv-c1cva5    deidv-c1cvi6    deidv-c1cwe6    deidv-c1cwk6    deidv-c1cxm7    deidv-c1cy72    deidv-c1d002    deidv-c1d2d8    deidv-c1d3d8    deidv-c1d3p0    deidv-c1cxw0    deidv-c1d2x1    deidv-c1cvt7
Gene_locus_frgt deidv-c1d0j6

Citations formats

de Groot A, Dulermo R, Ortet P, Blanchard L, Guerin P, Fernandez B, Vacherie B, Dossat C, Jolivet E, Siguier P, Chandler M, Barakat M, Dedieu A, Barbe V, Heulin T, Sommer S, Achouak W, Armengaud J (2009)
Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti
PLoS Genet 5 :e1000434

de Groot A, Dulermo R, Ortet P, Blanchard L, Guerin P, Fernandez B, Vacherie B, Dossat C, Jolivet E, Siguier P, Chandler M, Barakat M, Dedieu A, Barbe V, Heulin T, Sommer S, Achouak W, Armengaud J (2009)
PLoS Genet 5 :e1000434