Barbe V

References (56)

Title : Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic - Bennke_2016_Environ.Microbiol_18_4456
Author(s) : Bennke CM , Kruger K , Kappelmann L , Huang S , Gobet A , Schuler M , Barbe V , Fuchs BM , Michel G , Teeling H , Amann RI
Ref : Environ Microbiol , 18 :4456 , 2016
Abstract : Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes-affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here, we report on the analysis of further 174 fosmids from the same libraries. The combined, re-assembled dataset (226 contigs; 8.8 Mbp) suggests that planktonic Bacteroidetes at the oligotrophic southern station use more peptides and bacterial and animal polysaccharides, whereas Bacteroidetes at the polar station (East-Greenland Current) use more algal and plant polysaccharides. The latter agrees with higher abundances of algae and terrigenous organic matter, including plant material, at the polar station. Results were corroborated by in-depth bioinformatic analysis of 14 polysaccharide utilisation loci from both stations, suggesting laminarin-specificity for four and specificity for sulfated xylans for two loci. In addition, one locus from the polar station supported use of non-sulfated xylans and mannans, possibly of plant origin. While peptides likely represent a prime source of carbon for Bacteroidetes in open oceans, our data suggest that as yet unstudied clades of these Bacteroidetes have a surprisingly broad capacity for polysaccharide degradation. In particular, laminarin-specific PULs seem widespread and thus must be regarded as globally important.
ESTHER : Bennke_2016_Environ.Microbiol_18_4456
PubMedSearch : Bennke_2016_Environ.Microbiol_18_4456
PubMedID: 27348854
Gene_locus related to this paper: 9bact-a0a1b2ypb1

Title : Structural and functional partitioning of bread wheat chromosome 3B - Choulet_2014_Science_345_1249721
Author(s) : Choulet F , Alberti A , Theil S , Glover N , Barbe V , Daron J , Pingault L , Sourdille P , Couloux A , Paux E , Leroy P , Mangenot S , Guilhot N , Le Gouis J , Balfourier F , Alaux M , Jamilloux V , Poulain J , Durand C , Bellec A , Gaspin C , Safar J , Dolezel J , Rogers J , Vandepoele K , Aury JM , Mayer K , Berges H , Quesneville H , Wincker P , Feuillet C
Ref : Science , 345 :1249721 , 2014
Abstract : We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.
ESTHER : Choulet_2014_Science_345_1249721
PubMedSearch : Choulet_2014_Science_345_1249721
PubMedID: 25035497
Gene_locus related to this paper: wheat-a0a080yuw6 , wheat-w5d1z6 , wheat-a0a077rex4 , wheat-a0a077s1q2

Title : Comparative genomic analysis provides insights into the evolution and niche adaptation of marine Magnetospira sp. QH-2 strain - Ji_2014_Environ.Microbiol_16_525
Author(s) : Ji B , Zhang SD , Arnoux P , Rouy Z , Alberto F , Philippe N , Murat D , Zhang WJ , Rioux JB , Ginet N , Sabaty M , Mangenot S , Pradel N , Tian J , Yang J , Zhang L , Zhang W , Pan H , Henrissat B , Coutinho PM , Li Y , Xiao T , Medigue C , Barbe V , Pignol D , Talla E , Wu LF
Ref : Environ Microbiol , 16 :525 , 2014
Abstract : Magnetotactic bacteria (MTB) are capable of synthesizing intracellular organelles, the magnetosomes, that are membrane-bounded magnetite or greigite crystals arranged in chains. Although MTB are widely spread in various ecosystems, few axenic cultures are available, and only freshwater Magnetospirillum spp. have been genetically analysed. Here, we present the complete genome sequence of a marine magnetotactic spirillum, Magnetospira sp. QH-2. The high number of repeats and transposable elements account for the differences in QH-2 genome structure compared with other relatives. Gene cluster synteny and gene correlation analyses indicate that the insertion of the magnetosome island in the QH-2 genome occurred after divergence between freshwater and marine magnetospirilla. The presence of a sodium-quinone reductase, sodium transporters and other functional genes are evidence of the adaptive evolution of Magnetospira sp. QH-2 to the marine ecosystem. Genes well conserved among freshwater magnetospirilla for nitrogen fixation and assimilatory nitrate respiration are absent from the QH-2 genome. Unlike freshwater Magnetospirillum spp., marine Magnetospira sp. QH-2 neither has TonB and TonB-dependent receptors nor does it grow on trace amounts of iron. Taken together, our results show a distinct, adaptive evolution of Magnetospira sp. QH-2 to marine sediments in comparison with its closely related freshwater counterparts.
ESTHER : Ji_2014_Environ.Microbiol_16_525
PubMedSearch : Ji_2014_Environ.Microbiol_16_525
PubMedID: 23841906
Gene_locus related to this paper: 9prot-w6k5m7 , 9prot-w6khf2 , 9prot-w6kbu9

Title : Genomic analysis of smooth tubercle bacilli provides insights into ancestry and pathoadaptation of Mycobacterium tuberculosis - Supply_2013_Nat.Genet_45_172
Author(s) : Supply P , Marceau M , Mangenot S , Roche D , Rouanet C , Khanna V , Majlessi L , Criscuolo A , Tap J , Pawlik A , Fiette L , Orgeur M , Fabre M , Parmentier C , Frigui W , Simeone R , Boritsch EC , Debrie AS , Willery E , Walker D , Quail MA , Ma L , Bouchier C , Salvignol G , Sayes F , Cascioferro A , Seemann T , Barbe V , Locht C , Gutierrez MC , Leclerc C , Bentley SD , Stinear TP , Brisse S , Medigue C , Parkhill J , Cruveiller S , Brosch R
Ref : Nat Genet , 45 :172 , 2013
Abstract : Global spread and limited genetic variation are hallmarks of M. tuberculosis, the agent of human tuberculosis. In contrast, Mycobacterium canettii and related tubercle bacilli that also cause human tuberculosis and exhibit unusual smooth colony morphology are restricted to East Africa. Here, we sequenced and analyzed the whole genomes of five representative strains of smooth tubercle bacilli (STB) using Sanger (4-5x coverage), 454/Roche (13-18x coverage) and/or Illumina DNA sequencing (45-105x coverage). We show that STB isolates are highly recombinogenic and evolutionarily early branching, with larger genome sizes, higher rates of genetic variation, fewer molecular scars and distinct CRISPR-Cas systems relative to M. tuberculosis. Despite the differences, all tuberculosis-causing mycobacteria share a highly conserved core genome. Mouse infection experiments showed that STB strains are less persistent and virulent than M. tuberculosis. We conclude that M. tuberculosis emerged from an ancestral STB-like pool of mycobacteria by gain of persistence and virulence mechanisms, and we provide insights into the molecular events involved.
ESTHER : Supply_2013_Nat.Genet_45_172
PubMedSearch : Supply_2013_Nat.Genet_45_172
PubMedID: 23291586
Gene_locus related to this paper: mycmm-b2ht49 , myctu-cut3 , myctu-cutas1 , myctu-cutas2 , myctu-Rv1069c , myctu-RV1215C , myctu-RV1758 , myctu-Rv2045c , myctu-RV3452 , myctu-RV3724 , myctu-Rv3802c

Title : Genome Sequence of Halomonas sp. Strain A3H3, Isolated from Arsenic-Rich Marine Sediments - Koechler_2013_Genome.Announc_1_e00819
Author(s) : Koechler S , Plewniak F , Barbe V , Battaglia-Brunet F , Jost B , Joulian C , Philipps M , Vicaire S , Vincent S , Ye T , Bertin PN
Ref : Genome Announc , 1 : , 2013
Abstract : We report the genome sequence of Halomonas sp. strain A3H3, a bacterium with a high tolerance to arsenite, isolated from multicontaminated sediments of the l'Estaque harbor in Marseille, France. The genome is composed of a 5,489,893-bp chromosome and a 157,085-bp plasmid.
ESTHER : Koechler_2013_Genome.Announc_1_e00819
PubMedSearch : Koechler_2013_Genome.Announc_1_e00819
PubMedID: 24115546
Gene_locus related to this paper: 9gamm-t2l9f4 , 9gamm-t2l8b8

Title : Life in an arsenic-containing gold mine: genome and physiology of the autotrophic arsenite-oxidizing bacterium rhizobium sp. NT-26 - Andres_2013_Genome.Biol.Evol_5_934
Author(s) : Andres J , Arsene-Ploetze F , Barbe V , Brochier-Armanet C , Cleiss-Arnold J , Coppee JY , Dillies MA , Geist L , Joublin A , Koechler S , Lassalle F , Marchal M , Medigue C , Muller D , Nesme X , Plewniak F , Proux C , Ramirez-Bahena MH , Schenowitz C , Sismeiro O , Vallenet D , Santini JM , Bertin PN
Ref : Genome Biol Evol , 5 :934 , 2013
Abstract : Arsenic is widespread in the environment and its presence is a result of natural or anthropogenic activities. Microbes have developed different mechanisms to deal with toxic compounds such as arsenic and this is to resist or metabolize the compound. Here, we present the first reference set of genomic, transcriptomic and proteomic data of an Alphaproteobacterium isolated from an arsenic-containing goldmine: Rhizobium sp. NT-26. Although phylogenetically related to the plant-associated bacteria, this organism has lost the major colonizing capabilities needed for symbiosis with legumes. In contrast, the genome of Rhizobium sp. NT-26 comprises a megaplasmid containing the various genes, which enable it to metabolize arsenite. Remarkably, although the genes required for arsenite oxidation and flagellar motility/biofilm formation are carried by the megaplasmid and the chromosome, respectively, a coordinate regulation of these two mechanisms was observed. Taken together, these processes illustrate the impact environmental pressure can have on the evolution of bacterial genomes, improving the fitness of bacterial strains by the acquisition of novel functions.
ESTHER : Andres_2013_Genome.Biol.Evol_5_934
PubMedSearch : Andres_2013_Genome.Biol.Evol_5_934
PubMedID: 23589360
Gene_locus related to this paper: rhisp-l0nkx2 , 9rhiz-l0net0

Title : The rhizome of the multidrug-resistant Enterobacter aerogenes genome reveals how new killer bugs are created because of a sympatric lifestyle - Diene_2013_Mol.Biol.Evol_30_369
Author(s) : Diene SM , Merhej V , Henry M , El Filali A , Roux V , Robert C , Azza S , Gavory F , Barbe V , La Scola B , Raoult D , Rolain JM
Ref : Molecular Biology Evolution , 30 :369 , 2013
Abstract : Here, we sequenced the 5,419,609 bp circular genome of an Enterobacter aerogenes clinical isolate that killed a patient and was resistant to almost all current antibiotics (except gentamicin) commonly used to treat Enterobacterial infections, including colistin. Genomic and phylogenetic analyses explain the discrepancies of this bacterium and show that its core genome originates from another genus, Klebsiella. Atypical characteristics of this bacterium (i.e., motility, presence of ornithine decarboxylase, and lack of urease activity) are attributed to genomic mosaicism, by acquisition of additional genes, such as the complete 60,582 bp flagellar assembly operon acquired "en bloc" from the genus Serratia. The genealogic tree of the 162,202 bp multidrug-resistant conjugative plasmid shows that it is a chimera of transposons and integrative conjugative elements from various bacterial origins, resembling a rhizome. Moreover, we demonstrate biologically that a G53S mutation in the pmrA gene results in colistin resistance. E. aerogenes has a large RNA population comprising 8 rRNA operons and 87 cognate tRNAs that have the ability to translate transferred genes that use different codons, as exemplified by the significantly different codon usage between genes from the core genome and the "mobilome." On the basis of our findings, the evolution of this bacterium to become a "killer bug" with new genomic repertoires was from three criteria that are "opportunity, power, and usage" to indicate a sympatric lifestyle: "opportunity" to meet other bacteria and exchange foreign sequences since this bacteria was similar to sympatric bacteria; "power" to integrate these foreign sequences such as the acquisition of several mobile genetic elements (plasmids, integrative conjugative element, prophages, transposons, flagellar assembly system, etc.) found in his genome; and "usage" to have the ability to translate these sequences including those from rare codons to serve as a translator of foreign languages.
ESTHER : Diene_2013_Mol.Biol.Evol_30_369
PubMedSearch : Diene_2013_Mol.Biol.Evol_30_369
PubMedID: 23071100
Gene_locus related to this paper: entak-g0ea28 , entae-l8bq29 , entae-y1gc81 , entae-y1evy4

Title : Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads - Darrasse_2013_BMC.Genomics_14_761
Author(s) : Darrasse A , Carrere S , Barbe V , Boureau T , Arrieta-Ortiz ML , Bonneau S , Briand M , Brin C , Cociancich S , Durand K , Fouteau S , Gagnevin L , Guerin F , Guy E , Indiana A , Koebnik R , Lauber E , Munoz A , Noel LD , Pieretti I , Poussier S , Pruvost O , Robene-Soustrade I , Rott P , Royer M , Serres-Giardi L , Szurek B , Van Sluys MA , Verdier V , Verniere C , Arlat M , Manceau C , Jacques MA
Ref : BMC Genomics , 14 :761 , 2013
Abstract : BACKGROUND: Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences.
RESULTS: Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations.
CONCLUSIONS: This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.
ESTHER : Darrasse_2013_BMC.Genomics_14_761
PubMedSearch : Darrasse_2013_BMC.Genomics_14_761
PubMedID: 24195767
Gene_locus related to this paper: xanax-estA1 , xanax-XAC1213 , xanax-XAC2987 , xanax-XAC3315 , xanax-XAC4055 , xanor-q5h5n1

Title : Genome sequence of the human- and animal-pathogenic strain Nocardia cyriacigeorgica GUH-2 - Zoropogui_2012_J.Bacteriol_194_2098
Author(s) : Zoropogui A , Pujic P , Normand P , Barbe V , Beaman B , Beaman L , Boiron P , Colinon C , Deredjian A , Graindorge A , Mangenot S , Nazaret S , Neto M , Petit S , Roche D , Vallenet D , Rodriguez-Nava V , Richard Y , Cournoyer B , Blaha D
Ref : Journal of Bacteriology , 194 :2098 , 2012
Abstract : The pathogenic strain Nocardia cyriacigeorgica GUH-2 was isolated from a fatal human nocardiosis case, and its genome was sequenced. The complete genomic sequence of this strain contains 6,194,645 bp, an average G+C content of 68.37%, and no plasmids. We also identified several protein-coding genes to which N. cyriacigeorgica's virulence can potentially be attributed.
ESTHER : Zoropogui_2012_J.Bacteriol_194_2098
PubMedSearch : Zoropogui_2012_J.Bacteriol_194_2098
PubMedID: 22461543
Gene_locus related to this paper: noccg-h6r1f5 , noccg-h6r6y9 , noccg-h6r479 , noccg-h6rch6 , noccg-h6r8b1 , noccg-h6r6b6 , noccg-h6r249 , noccg-h6r9n3 , noccg-h6r4q8 , noccg-h6rbg1 , noccg-h6r7g5 , noccg-h6r481 , noccg-h6r0w3 , noccg-h6r3f1

Title : Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti - Cornillot_2012_Nucleic.Acids.Res_40_9102
Author(s) : Cornillot E , Hadj-Kaddour K , Dassouli A , Noel B , Ranwez V , Vacherie B , Augagneur Y , Bres V , Duclos A , Randazzo S , Carcy B , Debierre-Grockiego F , Delbecq S , Moubri-Menage K , Shams-Eldin H , Usmani-Brown S , Bringaud F , Wincker P , Vivares CP , Schwarz RT , Schetters TP , Krause PJ , Gorenflot A , Berry V , Barbe V , Ben Mamoun C
Ref : Nucleic Acids Research , 40 :9102 , 2012
Abstract : We have sequenced the genome of the emerging human pathogen Babesia microti and compared it with that of other protozoa. B. microti has the smallest nuclear genome among all Apicomplexan parasites sequenced to date with three chromosomes encoding approximately 3500 polypeptides, several of which are species specific. Genome-wide phylogenetic analyses indicate that B. microti is significantly distant from all species of Babesidae and Theileridae and defines a new clade in the phylum Apicomplexa. Furthermore, unlike all other Apicomplexa, its mitochondrial genome is circular. Genome-scale reconstruction of functional networks revealed that B. microti has the minimal metabolic requirement for intraerythrocytic protozoan parasitism. B. microti multigene families differ from those of other protozoa in both the copy number and organization. Two lateral transfer events with significant metabolic implications occurred during the evolution of this parasite. The genomic sequencing of B. microti identified several targets suitable for the development of diagnostic assays and novel therapies for human babesiosis.
ESTHER : Cornillot_2012_Nucleic.Acids.Res_40_9102
PubMedSearch : Cornillot_2012_Nucleic.Acids.Res_40_9102
PubMedID: 22833609
Gene_locus related to this paper: babmi-i7isy5

Title : Genome sequence of radiation-resistant Modestobacter marinus strain BC501, a representative actinobacterium that thrives on calcareous stone surfaces - Normand_2012_J.Bacteriol_194_4773
Author(s) : Normand P , Gury J , Pujic P , Chouaia B , Crotti E , Brusetti L , Daffonchio D , Vacherie B , Barbe V , Medigue C , Calteau A , Ghodhbane-Gtari F , Essoussi I , Nouioui I , Abbassi-Ghozzi I , Gtari M
Ref : Journal of Bacteriology , 194 :4773 , 2012
Abstract : Here we report the full genome sequence of Modestobacter marinus strain BC501, an actinobacterial isolate that thrives on stone surfaces. The generated chromosome is circular, with a length of 5.57 Mb and a G+C content of 74.13%, containing 5,445 protein-coding genes, 48 tRNAs, and 3 ribosomal operons.
ESTHER : Normand_2012_J.Bacteriol_194_4773
PubMedSearch : Normand_2012_J.Bacteriol_194_4773
PubMedID: 22887672
Gene_locus related to this paper: modmb-i4eyb4 , modmb-i4f114 , modmb-i4f525 , modmb-i4f4c1

Title : Genome sequence of the marine bacterium Marinobacter hydrocarbonoclasticus SP17, which forms biofilms on hydrophobic organic compounds - Grimaud_2012_J.Bacteriol_194_3539
Author(s) : Grimaud R , Ghiglione JF , Cagnon C , Lauga B , Vaysse PJ , Rodriguez-Blanco A , Mangenot S , Cruveiller S , Barbe V , Duran R , Wu LF , Talla E , Bonin P , Michotey V
Ref : Journal of Bacteriology , 194 :3539 , 2012
Abstract : Marinobacter hydrocarbonoclasticus SP17 forms biofilms specifically at the interface between water and hydrophobic organic compounds (HOCs) that are used as carbon and energy sources. Biofilm formation at the HOC-water interface has been recognized as a strategy to overcome the low availability of these nearly water-insoluble substrates. Here, we present the genome sequence of SP17, which could provide further insights into the mechanisms of enhancement of HOCs assimilation through biofilm formation.
ESTHER : Grimaud_2012_J.Bacteriol_194_3539
PubMedSearch : Grimaud_2012_J.Bacteriol_194_3539
PubMedID: 22689231
Gene_locus related to this paper: marav-a1u338 , marhy-h8w622 , marhy-h8w685 , marav-a1u1j6

Title : Genome sequence of the haloalkaliphilic methanotrophic bacterium Methylomicrobium alcaliphilum 20Z - Vuilleumier_2012_J.Bacteriol_194_551
Author(s) : Vuilleumier S , Khmelenina VN , Bringel F , Reshetnikov AS , Lajus A , Mangenot S , Rouy Z , Op den Camp HJ , Jetten MS , DiSpirito AA , Dunfield P , Klotz MG , Semrau JD , Stein LY , Barbe V , Medigue C , Trotsenko YA , Kalyuzhnaya MG
Ref : Journal of Bacteriology , 194 :551 , 2012
Abstract : Methylomicrobium strains are widespread in saline environments. Here, we report the complete genome sequence of Methylomicrobium alcaliphilum 20Z, a haloalkaliphilic methanotrophic bacterium, which will provide the basis for detailed characterization of the core pathways of both single-carbon metabolism and responses to osmotic and high-pH stresses. Final assembly of the genome sequence revealed that this bacterium contains a 128-kb plasmid, making M. alcaliphilum 20Z the first methanotrophic bacterium of known genome sequence for which a plasmid has been reported.
ESTHER : Vuilleumier_2012_J.Bacteriol_194_551
PubMedSearch : Vuilleumier_2012_J.Bacteriol_194_551
PubMedID: 22207753

Title : Pichia sorbitophila, an Interspecies Yeast Hybrid, Reveals Early Steps of Genome Resolution After Polyploidization - Louis_2012_G3.(Bethesda)_2_299
Author(s) : Louis VL , Despons L , Friedrich A , Martin T , Durrens P , Casaregola S , Neuveglise C , Fairhead C , Marck C , Cruz JA , Straub ML , Kugler V , Sacerdot C , Uzunov Z , Thierry A , Weiss S , Bleykasten C , De Montigny J , Jacques N , Jung P , Lemaire M , Mallet S , Morel G , Richard GF , Sarkar A , Savel G , Schacherer J , Seret ML , Talla E , Samson G , Jubin C , Poulain J , Vacherie B , Barbe V , Pelletier E , Sherman DJ , Westhof E , Weissenbach J , Baret PV , Wincker P , Gaillardin C , Dujon B , Souciet JL
Ref : G3 (Bethesda) , 2 :299 , 2012
Abstract : Polyploidization is an important process in the evolution of eukaryotic genomes, but ensuing molecular mechanisms remain to be clarified. Autopolyploidization or whole-genome duplication events frequently are resolved in resulting lineages by the loss of single genes from most duplicated pairs, causing transient gene dosage imbalance and accelerating speciation through meiotic infertility. Allopolyploidization or formation of interspecies hybrids raises the problem of genetic incompatibility (Bateson-Dobzhansky-Muller effect) and may be resolved by the accumulation of mutational changes in resulting lineages. In this article, we show that an osmotolerant yeast species, Pichia sorbitophila, recently isolated in a concentrated sorbitol solution in industry, illustrates this last situation. Its genome is a mosaic of homologous and homeologous chromosomes, or parts thereof, that corresponds to a recently formed hybrid in the process of evolution. The respective parental contributions to this genome were characterized using existing variations in GC content. The genomic changes that occurred during the short period since hybrid formation were identified (e.g., loss of heterozygosity, unilateral loss of rDNA, reciprocal exchange) and distinguished from those undergone by the two parental genomes after separation from their common ancestor (i.e., NUMT (NUclear sequences of MiTochondrial origin) insertions, gene acquisitions, gene location movements, reciprocal translocation). We found that the physiological characteristics of this new yeast species are determined by specific but unequal contributions of its two parents, one of which could be identified as very closely related to an extant Pichia farinosa strain.
ESTHER : Louis_2012_G3.(Bethesda)_2_299
PubMedSearch : Louis_2012_G3.(Bethesda)_2_299
PubMedID: 22384408
Gene_locus related to this paper: picso-g8ycc9 , picso-g8yet0 , picso-g8yb96 , picso-g8ym39 , erecy-g8jrp5 , picso-g8y652

Title : Ralstonia syzygii, the Blood Disease Bacterium and some Asian R. solanacearum strains form a single genomic species despite divergent lifestyles - Remenant_2011_PLoS.One_6_e24356
Author(s) : Remenant B , de Cambiaire JC , Cellier G , Jacobs JM , Mangenot S , Barbe V , Lajus A , Vallenet D , Medigue C , Fegan M , Allen C , Prior P
Ref : PLoS ONE , 6 :e24356 , 2011
Abstract : The Ralstonia solanacearum species complex includes R. solanacearum, R. syzygii, and the Blood Disease Bacterium (BDB). All colonize plant xylem vessels and cause wilt diseases, but with significant biological differences. R. solanacearum is a soilborne bacterium that infects the roots of a broad range of plants. R. syzygii causes Sumatra disease of clove trees and is actively transmitted by cercopoid insects. BDB is also pathogenic to a single host, banana, and is transmitted by pollinating insects. Sequencing and DNA-DNA hybridization studies indicated that despite their phenotypic differences, these three plant pathogens are actually very closely related, falling into the Phylotype IV subgroup of the R. solanacearum species complex. To better understand the relationships among these bacteria, we sequenced and annotated the genomes of R. syzygii strain R24 and BDB strain R229. These genomes were compared to strain PSI07, a closely related Phylotype IV tomato isolate of R. solanacearum, and to five additional R. solanacearum genomes. Whole-genome comparisons confirmed previous phylogenetic results: the three phylotype IV strains share more and larger syntenic regions with each other than with other R. solanacearum strains. Furthermore, the genetic distances between strains, assessed by an in-silico equivalent of DNA-DNA hybridization, unambiguously showed that phylotype IV strains of BDB, R. syzygii and R. solanacearum form one genomic species. Based on these comprehensive data we propose a revision of the taxonomy of the R. solanacearum species complex. The BDB and R. syzygii genomes encoded no obvious unique metabolic capacities and contained no evidence of horizontal gene transfer from bacteria occupying similar niches. Genes specific to R. syzygii and BDB were almost all of unknown function or extrachromosomal origin. Thus, the pathogenic life-styles of these organisms are more probably due to ecological adaptation and genomic convergence during vertical evolution than to the acquisition of DNA by horizontal transfer.
ESTHER : Remenant_2011_PLoS.One_6_e24356
PubMedSearch : Remenant_2011_PLoS.One_6_e24356
PubMedID: 21931687
Gene_locus related to this paper: 9rals-g2zrs4 , 9rals-g3a1g4 , ralsl-a0a072zy50 , 9rals-g3a7g7

Title : Complete genome sequence of a beneficial plant root-associated bacterium, Pseudomonas brassicacearum - Ortet_2011_J.Bacteriol_193_3146
Author(s) : Ortet P , Barakat M , Lalaouna D , Fochesato S , Barbe V , Vacherie B , Santaella C , Heulin T , Achouak W
Ref : Journal of Bacteriology , 193 :3146 , 2011
Abstract : To shed light on the genetic equipment of the beneficial plant-associated bacterium Pseudomonas brassicacearum, we sequenced the whole genome of the strain NFM421. Its genome consists of one chromosome equipped with a repertoire of factors beneficial for plant growth. In addition, a complete type III secretion system and two complete type VI secretion systems were identified. We report here the first genome sequence of this species.
ESTHER : Ortet_2011_J.Bacteriol_193_3146
PubMedSearch : Ortet_2011_J.Bacteriol_193_3146
PubMedID: 21515771
Gene_locus related to this paper: psebn-f2k6b7 , psebn-f2k6e0 , psebn-f2kcw9 , psebn-f2kd12 , psebn-f2khr7 , psebr-lipa , psepf-PHAZ , psebn-f2k9f6 , 9psed-s6i647

Title : Azospirillum genomes reveal transition of bacteria from aquatic to terrestrial environments - Wisniewski-Dye_2011_PLoS.Genet_7_e1002430
Author(s) : Wisniewski-Dye F , Borziak K , Khalsa-Moyers G , Alexandre G , Sukharnikov LO , Wuichet K , Hurst GB , McDonald WH , Robertson JS , Barbe V , Calteau A , Rouy Z , Mangenot S , Prigent-Combaret C , Normand P , Boyer M , Siguier P , Dessaux Y , Elmerich C , Condemine G , Krishnen G , Kennedy I , Paterson AH , Gonzalez V , Mavingui P , Zhulin IB
Ref : PLoS Genet , 7 :e1002430 , 2011
Abstract : Fossil records indicate that life appeared in marine environments approximately 3.5 billion years ago (Gyr) and transitioned to terrestrial ecosystems nearly 2.5 Gyr. Sequence analysis suggests that "hydrobacteria" and "terrabacteria" might have diverged as early as 3 Gyr. Bacteria of the genus Azospirillum are associated with roots of terrestrial plants; however, virtually all their close relatives are aquatic. We obtained genome sequences of two Azospirillum species and analyzed their gene origins. While most Azospirillum house-keeping genes have orthologs in its close aquatic relatives, this lineage has obtained nearly half of its genome from terrestrial organisms. The majority of genes encoding functions critical for association with plants are among horizontally transferred genes. Our results show that transition of some aquatic bacteria to terrestrial habitats occurred much later than the suggested initial divergence of hydro- and terrabacterial clades. The birth of the genus Azospirillum approximately coincided with the emergence of vascular plants on land.
ESTHER : Wisniewski-Dye_2011_PLoS.Genet_7_e1002430
PubMedSearch : Wisniewski-Dye_2011_PLoS.Genet_7_e1002430
PubMedID: 22216014
Gene_locus related to this paper: azobr-g8al65 , azol4-g7zc95 , azol4-g7zc98 , azol4-g7zfp9 , azol4-g7zcr5 , azobr-g8b0i6

Title : The Medicago genome provides insight into the evolution of rhizobial symbioses - Young_2011_Nature_480_520
Author(s) : Young ND , Debelle F , Oldroyd GE , Geurts R , Cannon SB , Udvardi MK , Benedito VA , Mayer KF , Gouzy J , Schoof H , Van de Peer Y , Proost S , Cook DR , Meyers BC , Spannagl M , Cheung F , De Mita S , Krishnakumar V , Gundlach H , Zhou S , Mudge J , Bharti AK , Murray JD , Naoumkina MA , Rosen B , Silverstein KA , Tang H , Rombauts S , Zhao PX , Zhou P , Barbe V , Bardou P , Bechner M , Bellec A , Berger A , Berges H , Bidwell S , Bisseling T , Choisne N , Couloux A , Denny R , Deshpande S , Dai X , Doyle JJ , Dudez AM , Farmer AD , Fouteau S , Franken C , Gibelin C , Gish J , Goldstein S , Gonzalez AJ , Green PJ , Hallab A , Hartog M , Hua A , Humphray SJ , Jeong DH , Jing Y , Jocker A , Kenton SM , Kim DJ , Klee K , Lai H , Lang C , Lin S , Macmil SL , Magdelenat G , Matthews L , McCorrison J , Monaghan EL , Mun JH , Najar FZ , Nicholson C , Noirot C , O'Bleness M , Paule CR , Poulain J , Prion F , Qin B , Qu C , Retzel EF , Riddle C , Sallet E , Samain S , Samson N , Sanders I , Saurat O , Scarpelli C , Schiex T , Segurens B , Severin AJ , Sherrier DJ , Shi R , Sims S , Singer SR , Sinharoy S , Sterck L , Viollet A , Wang BB , Wang K , Wang M , Wang X , Warfsmann J , Weissenbach J , White DD , White JD , Wiley GB , Wincker P , Xing Y , Yang L , Yao Z , Ying F , Zhai J , Zhou L , Zuber A , Denarie J , Dixon RA , May GD , Schwartz DC , Rogers J , Quetier F , Town CD , Roe BA
Ref : Nature , 480 :520 , 2011
Abstract : Legumes (Fabaceae or Leguminosae) are unique among cultivated plants for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process that takes place in a specialized structure known as the nodule. Legumes belong to one of the two main groups of eurosids, the Fabidae, which includes most species capable of endosymbiotic nitrogen fixation. Legumes comprise several evolutionary lineages derived from a common ancestor 60 million years ago (Myr ago). Papilionoids are the largest clade, dating nearly to the origin of legumes and containing most cultivated species. Medicago truncatula is a long-established model for the study of legume biology. Here we describe the draft sequence of the M. truncatula euchromatin based on a recently completed BAC assembly supplemented with Illumina shotgun sequence, together capturing approximately 94% of all M. truncatula genes. A whole-genome duplication (WGD) approximately 58 Myr ago had a major role in shaping the M. truncatula genome and thereby contributed to the evolution of endosymbiotic nitrogen fixation. Subsequent to the WGD, the M. truncatula genome experienced higher levels of rearrangement than two other sequenced legumes, Glycine max and Lotus japonicus. M. truncatula is a close relative of alfalfa (Medicago sativa), a widely cultivated crop with limited genomics tools and complex autotetraploid genetics. As such, the M. truncatula genome sequence provides significant opportunities to expand alfalfa's genomic toolbox.
ESTHER : Young_2011_Nature_480_520
PubMedSearch : Young_2011_Nature_480_520
PubMedID: 22089132
Gene_locus related to this paper: medtr-b7fki4 , medtr-b7fmi1 , medtr-g7itl1 , medtr-g7iu67 , medtr-g7izm0 , medtr-g7j641 , medtr-g7jtf8 , medtr-g7jtg2 , medtr-g7jtg4 , medtr-g7kem3 , medtr-g7kml3 , medtr-g7ksx5 , medtr-g7leb3 , medtr-q1s5d8 , medtr-q1s9m3 , medtr-q1t171 , medtr-g7k9e1 , medtr-g7k9e3 , medtr-g7k9e5 , medtr-g7k9e8 , medtr-g7k9e9 , medtr-g7lbp2 , medtr-g7lch3 , medtr-g7ib94 , medtr-g7ljk8 , medtr-g7i6w5 , medtr-g7kvg4 , medtr-g7iam1 , medtr-g7iam3 , medtr-g7l754 , medtr-g7jr41 , medtr-g7l4f5 , medtr-g7l755 , medtr-a0a072vyl4 , medtr-g7jwk8 , medtr-a0a072vhg0 , medtr-a0a072vrv9 , medtr-g7kmk5 , medtr-a0a072uuf6 , medtr-a0a072urp3 , medtr-g7zzc3 , medtr-g7ie19 , medtr-g7kst7 , medtr-a0a072u5k5 , medtr-a0a072v056 , medtr-scp1 , medtr-g7kyn0 , medtr-g7inw6 , medtr-g7j3q3

Title : Complete genome sequence of Streptomyces cattleya NRRL 8057, a producer of antibiotics and fluorometabolites - Barbe_2011_J.Bacteriol_193_5055
Author(s) : Barbe V , Bouzon M , Mangenot S , Badet B , Poulain J , Segurens B , Vallenet D , Marliere P , Weissenbach J
Ref : Journal of Bacteriology , 193 :5055 , 2011
Abstract : Streptomyces cattleya, a producer of the antibiotics thienamycin and cephamycin C, is one of the rare bacteria known to synthesize fluorinated metabolites. The genome consists of two linear replicons. The genes involved in fluorine metabolism and in the biosynthesis of the antibiotic thienamycin were mapped on both replicons.
ESTHER : Barbe_2011_J.Bacteriol_193_5055
PubMedSearch : Barbe_2011_J.Bacteriol_193_5055
PubMedID: 21868806
Gene_locus related to this paper: stren-f8jjf8 , stren-f8jk65 , stren-f8jly2 , stren-f8jm04 , stren-f8juv4 , stren-f8k466 , stren-f8jvj2 , stren-f8k4s8

Title : PCR-based identification of Klebsiella pneumoniae subsp. rhinoscleromatis, the agent of rhinoscleroma - Fevre_2011_PLoS.Negl.Trop.Dis_5_e1052
Author(s) : Fevre C , Passet V , Deletoile A , Barbe V , Frangeul L , Almeida AS , Sansonetti P , Tournebize R , Brisse S
Ref : PLoS Negl Trop Dis , 5 :e1052 , 2011
Abstract : Rhinoscleroma is a chronic granulomatous infection of the upper airways caused by the bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. The disease is endemic in tropical and subtropical areas, but its diagnosis remains difficult. As a consequence, and despite available antibiotherapy, some patients evolve advanced stages that can lead to disfiguration, severe respiratory impairment and death by anoxia. Because identification of the etiologic agent is crucial for the definitive diagnosis of the disease, the aim of this study was to develop two simple PCR assays. We took advantage of the fact that all Klebsiella pneumoniae subsp. rhinoscleromatis isolates are (i) of capsular serotype K3; and (ii) belong to a single clone with diagnostic single nucleotide polymorphisms (SNP). The complete sequence of the genomic region comprising the capsular polysaccharide synthesis (cps) gene cluster was determined. Putative functions of the 21 genes identified were consistent with the structure of the K3 antigen. The K3-specific sequence of gene Kr11509 (wzy) was exploited to set up a PCR test, which was positive for 40 K3 strains but negative when assayed on the 76 other Klebsiella capsular types. Further, to discriminate Klebsiella pneumoniae subsp. rhinoscleromatis from other K3 Klebsiella strains, a specific PCR assay was developed based on diagnostic SNPs in the phosphate porin gene phoE. This work provides rapid and simple molecular tools to confirm the diagnostic of rhinoscleroma, which should improve patient care as well as knowledge on the prevalence and epidemiology of rhinoscleroma.
ESTHER : Fevre_2011_PLoS.Negl.Trop.Dis_5_e1052
PubMedSearch : Fevre_2011_PLoS.Negl.Trop.Dis_5_e1052
PubMedID: 21629720
Gene_locus related to this paper: klep7-a6t9v6 , klep7-y1077 , klepn-w8uta0

Title : Complete genome sequence of the fish pathogen Flavobacterium branchiophilum - Touchon_2011_Appl.Environ.Microbiol_77_7656
Author(s) : Touchon M , Barbier P , Bernardet JF , Loux V , Vacherie B , Barbe V , Rocha EP , Duchaud E
Ref : Applied Environmental Microbiology , 77 :7656 , 2011
Abstract : Members of the genus Flavobacterium occur in a variety of ecological niches and represent an interesting diversity of lifestyles. Flavobacterium branchiophilum is the main causative agent of bacterial gill disease, a severe condition affecting various cultured freshwater fish species worldwide, in particular salmonids in Canada and Japan. We report here the complete genome sequence of strain FL-15 isolated from a diseased sheatfish (Silurus glanis) in Hungary. The analysis of the F. branchiophilum genome revealed putative mechanisms of pathogenicity strikingly different from those of the other, closely related fish pathogen Flavobacterium psychrophilum, including the first cholera-like toxin in a non-Proteobacteria and a wealth of adhesins. The comparison with available genomes of other Flavobacterium species revealed a small genome size, large differences in chromosome organization, and fewer rRNA and tRNA genes, in line with its more fastidious growth. In addition, horizontal gene transfer shaped the evolution of F. branchiophilum, as evidenced by its virulence factors, genomic islands, and CRISPR (clustered regularly interspaced short palindromic repeats) systems. Further functional analysis should help in the understanding of host-pathogen interactions and in the development of rational diagnostic tools and control strategies in fish farms.
ESTHER : Touchon_2011_Appl.Environ.Microbiol_77_7656
PubMedSearch : Touchon_2011_Appl.Environ.Microbiol_77_7656
PubMedID: 21926215
Gene_locus related to this paper: flabf-g2z3q6

Title : Structure, function, and evolution of the Thiomonas spp. genome - Arsene-Ploetze_2010_PLoS.Genet_6_e1000859
Author(s) : Arsene-Ploetze F , Koechler S , Marchal M , Coppee JY , Chandler M , Bonnefoy V , Brochier-Armanet C , Barakat M , Barbe V , Battaglia-Brunet F , Bruneel O , Bryan CG , Cleiss-Arnold J , Cruveiller S , Erhardt M , Heinrich-Salmeron A , Hommais F , Joulian C , Krin E , Lieutaud A , Lievremont D , Michel C , Muller D , Ortet P , Proux C , Siguier P , Roche D , Rouy Z , Salvignol G , Slyemi D , Talla E , Weiss S , Weissenbach J , Medigue C , Bertin PN
Ref : PLoS Genet , 6 :e1000859 , 2010
Abstract : Bacteria of the Thiomonas genus are ubiquitous in extreme environments, such as arsenic-rich acid mine drainage (AMD). The genome of one of these strains, Thiomonas sp. 3As, was sequenced, annotated, and examined, revealing specific adaptations allowing this bacterium to survive and grow in its highly toxic environment. In order to explore genomic diversity as well as genetic evolution in Thiomonas spp., a comparative genomic hybridization (CGH) approach was used on eight different strains of the Thiomonas genus, including five strains of the same species. Our results suggest that the Thiomonas genome has evolved through the gain or loss of genomic islands and that this evolution is influenced by the specific environmental conditions in which the strains live.
ESTHER : Arsene-Ploetze_2010_PLoS.Genet_6_e1000859
PubMedSearch : Arsene-Ploetze_2010_PLoS.Genet_6_e1000859
PubMedID: 20195515
Gene_locus related to this paper: thik1-d5x539 , thia3-d6cv72 , thia3-d6clv8

Title : Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence - Fonknechten_2010_BMC.Genomics_11_555
Author(s) : Fonknechten N , Chaussonnerie S , Tricot S , Lajus A , Andreesen JR , Perchat N , Pelletier E , Gouyvenoux M , Barbe V , Salanoubat M , Le Paslier D , Weissenbach J , Cohen GN , Kreimeyer A
Ref : BMC Genomics , 11 :555 , 2010
Abstract : BACKGROUND: Clostridium sticklandii belongs to a cluster of non-pathogenic proteolytic clostridia which utilize amino acids as carbon and energy sources. Isolated by T.C. Stadtman in 1954, it has been generally regarded as a "gold mine" for novel biochemical reactions and is used as a model organism for studying metabolic aspects such as the Stickland reaction, coenzyme-B12- and selenium-dependent reactions of amino acids. With the goal of revisiting its carbon, nitrogen, and energy metabolism, and comparing studies with other clostridia, its genome has been sequenced and analyzed.
RESULTS: C. sticklandii is one of the best biochemically studied proteolytic clostridial species. Useful additional information has been obtained from the sequencing and annotation of its genome, which is presented in this paper. Besides, experimental procedures reveal that C. sticklandii degrades amino acids in a preferential and sequential way. The organism prefers threonine, arginine, serine, cysteine, proline, and glycine, whereas glutamate, aspartate and alanine are excreted. Energy conservation is primarily obtained by substrate-level phosphorylation in fermentative pathways. The reactions catalyzed by different ferredoxin oxidoreductases and the exergonic NADH-dependent reduction of crotonyl-CoA point to a possible chemiosmotic energy conservation via the Rnf complex. C. sticklandii possesses both the F-type and V-type ATPases. The discovery of an as yet unrecognized selenoprotein in the D-proline reductase operon suggests a more detailed mechanism for NADH-dependent D-proline reduction. A rather unusual metabolic feature is the presence of genes for all the enzymes involved in two different CO2-fixation pathways: C. sticklandii harbours both the glycine synthase/glycine reductase and the Wood-Ljungdahl pathways. This unusual pathway combination has retrospectively been observed in only four other sequenced microorganisms.
CONCLUSIONS: Analysis of the C. sticklandii genome and additional experimental procedures have improved our understanding of anaerobic amino acid degradation. Several specific metabolic features have been detected, some of which are very unusual for anaerobic fermenting bacteria. Comparative genomics has provided the opportunity to study the lifestyle of pathogenic and non-pathogenic clostridial species as well as to elucidate the difference in metabolic features between clostridia and other anaerobes.
ESTHER : Fonknechten_2010_BMC.Genomics_11_555
PubMedSearch : Fonknechten_2010_BMC.Genomics_11_555
PubMedID: 20937090

Title : Fine-scale evolution: genomic, phenotypic and ecological differentiation in two coexisting Salinibacter ruber strains - Pena_2010_ISME.J_4_882
Author(s) : Pena A , Teeling H , Huerta-Cepas J , Santos F , Yarza P , Brito-Echeverria J , Lucio M , Schmitt-Kopplin P , Meseguer I , Schenowitz C , Dossat C , Barbe V , Dopazo J , Rossello-Mora R , Schuler M , Glockner FO , Amann R , Gabaldon T , Anton J
Ref : Isme J , 4 :882 , 2010
Abstract : Genomic and metagenomic data indicate a high degree of genomic variation within microbial populations, although the ecological and evolutive meaning of this microdiversity remains unknown. Microevolution analyses, including genomic and experimental approaches, are so far very scarce for non-pathogenic bacteria. In this study, we compare the genomes, metabolomes and selected ecological traits of the strains M8 and M31 of the hyperhalophilic bacterium Salinibacter ruber that contain ribosomal RNA (rRNA) gene and intergenic regions that are identical in sequence and were simultaneously isolated from a Mediterranean solar saltern. Comparative analyses indicate that S. ruber genomes present a mosaic structure with conserved and hypervariable regions (HVRs). The HVRs or genomic islands, are enriched in transposases, genes related to surface properties, strain-specific genes and highly divergent orthologous. However, the many indels outside the HVRs indicate that genome plasticity extends beyond them. Overall, 10% of the genes encoded in the M8 genome are absent from M31 and could stem from recent acquisitions. S. ruber genomes also harbor 34 genes located outside HVRs that are transcribed during standard growth and probably derive from lateral gene transfers with Archaea preceding the M8/M31 divergence. Metabolomic analyses, phage susceptibility and competition experiments indicate that these genomic differences cannot be considered neutral from an ecological perspective. The results point to the avoidance of competition by micro-niche adaptation and response to viral predation as putative major forces that drive microevolution within these Salinibacter strains. In addition, this work highlights the extent of bacterial functional diversity and environmental adaptation, beyond the resolution of the 16S rRNA and internal transcribed spacers regions.
ESTHER : Pena_2010_ISME.J_4_882
PubMedSearch : Pena_2010_ISME.J_4_882
PubMedID: 20164864
Gene_locus related to this paper: salrd-q2rzy1 , salrd-q2s0e4 , salrd-q2s1i8

Title : The arthrobacter arilaitensis Re117 genome sequence reveals its genetic adaptation to the surface of cheese - Monnet_2010_PLoS.One_5_e15489
Author(s) : Monnet C , Loux V , Gibrat JF , Spinnler E , Barbe V , Vacherie B , Gavory F , Gourbeyre E , Siguier P , Chandler M , Elleuch R , Irlinger F , Vallaeys T
Ref : PLoS ONE , 5 :e15489 , 2010
Abstract : Arthrobacter arilaitensis is one of the major bacterial species found at the surface of cheeses, especially in smear-ripened cheeses, where it contributes to the typical colour, flavour and texture properties of the final product. The A. arilaitensis Re117 genome is composed of a 3,859,257 bp chromosome and two plasmids of 50,407 and 8,528 bp. The chromosome shares large regions of synteny with the chromosomes of three environmental Arthrobacter strains for which genome sequences are available: A. aurescens TC1, A. chlorophenolicus A6 and Arthrobacter sp. FB24. In contrast however, 4.92% of the A. arilaitensis chromosome is composed of ISs elements, a portion that is at least 15 fold higher than for the other Arthrobacter strains. Comparative genomic analyses reveal an extensive loss of genes associated with catabolic activities, presumably as a result of adaptation to the properties of the cheese surface habitat. Like the environmental Arthrobacter strains, A. arilaitensis Re117 is well-equipped with enzymes required for the catabolism of major carbon substrates present at cheese surfaces such as fatty acids, amino acids and lactic acid. However, A. arilaitensis has several specificities which seem to be linked to its adaptation to its particular niche. These include the ability to catabolize D-galactonate, a high number of glycine betaine and related osmolyte transporters, two siderophore biosynthesis gene clusters and a high number of Fe(3+)/siderophore transport systems. In model cheese experiments, addition of small amounts of iron strongly stimulated the growth of A. arilaitensis, indicating that cheese is a highly iron-restricted medium. We suggest that there is a strong selective pressure at the surface of cheese for strains with efficient iron acquisition and salt-tolerance systems together with abilities to catabolize substrates such as lactic acid, lipids and amino acids.
ESTHER : Monnet_2010_PLoS.One_5_e15489
PubMedSearch : Monnet_2010_PLoS.One_5_e15489
PubMedID: 21124797
Gene_locus related to this paper: artar-e1vw31 , artar-e1vwy8

Title : Genomes of three tomato pathogens within the Ralstonia solanacearum species complex reveal significant evolutionary divergence - Remenant_2010_BMC.Genomics_11_379
Author(s) : Remenant B , Coupat-Goutaland B , Guidot A , Cellier G , Wicker E , Allen C , Fegan M , Pruvost O , Elbaz M , Calteau A , Salvignol G , Mornico D , Mangenot S , Barbe V , Medigue C , Prior P
Ref : BMC Genomics , 11 :379 , 2010
Abstract : BACKGROUND: The Ralstonia solanacearum species complex includes thousands of strains pathogenic to an unusually wide range of plant species. These globally dispersed and heterogeneous strains cause bacterial wilt diseases, which have major socio-economic impacts. Pathogenicity is an ancestral trait in R. solanacearum and strains with high genetic variation can be subdivided into four phylotypes, correlating to isolates from Asia (phylotype I), the Americas (phylotype IIA and IIB), Africa (phylotype III) and Indonesia (phylotype IV). Comparison of genome sequences strains representative of this phylogenetic diversity can help determine which traits allow this bacterium to be such a pathogen of so many different plant species and how the bacteria survive in many different habitats.
RESULTS: The genomes of three tomato bacterial wilt pathogens, CFBP2957 (phy. IIA), CMR15 (phy. III) and PSI07 (phy. IV) were sequenced and manually annotated. These genomes were compared with those of three previously sequenced R. solanacearum strains: GMI1000 (tomato, phy. I), IPO1609 (potato, phy. IIB), and Molk2 (banana, phy. IIB). The major genomic features (size, G+C content, number of genes) were conserved across all of the six sequenced strains. Despite relatively high genetic distances (calculated from average nucleotide identity) and many genomic rearrangements, more than 60% of the genes of the megaplasmid and 70% of those on the chromosome are syntenic. The three new genomic sequences revealed the presence of several previously unknown traits, probably acquired by horizontal transfers, within the genomes of R. solanacearum, including a type IV secretion system, a rhi-type anti-mitotic toxin and two small plasmids. Genes involved in virulence appear to be evolving at a faster rate than the genome as a whole.
CONCLUSIONS: Comparative analysis of genome sequences and gene content confirmed the differentiation of R. solanacearum species complex strains into four phylotypes. Genetic distances between strains, in conjunction with CGH analysis of a larger set of strains, revealed differences great enough to consider reclassification of the R. solanacearum species complex into three species. The data are still too fragmentary to link genomic classification and phenotypes, but these new genome sequences identify a pan-genome more representative of the diversity in the R. solanancearum species complex.
ESTHER : Remenant_2010_BMC.Genomics_11_379
PubMedSearch : Remenant_2010_BMC.Genomics_11_379
PubMedID: 20550686
Gene_locus related to this paper: ralsl-d8p4i2 , ralso-d8njk6 , ralso-PCAD , ralso-PCAD2 , ralso-PHAZ , ralso-PHBC , ralso-RSC0439 , ralso-RSC0563 , ralso-RSC0604 , ralso-RSC1003 , ralso-RSC1344 , ralso-RSC1772 , ralso-RSC1804 , ralso-RSC1811 , ralso-RSC1887 , ralso-RSC2149 , ralso-RSC2228 , ralso-RSC2781 , ralso-RSC3346 , ralso-RSP0196 , ralso-RSP0795 , ralso-RSP1108 , ralso-RSP1248 , ralso-RSP1521 , ralsl-d8ny30 , ralsl-y0kx85 , ralsl-a0a072zy50

Title : Comparative genomic and proteomic analyses of two Mycoplasma agalactiae strains: clues to the macro- and micro-events that are shaping mycoplasma diversity - Nouvel_2010_BMC.Genomics_11_86
Author(s) : Nouvel LX , Sirand-Pugnet P , Marenda MS , Sagne E , Barbe V , Mangenot S , Schenowitz C , Jacob D , Barre A , Claverol S , Blanchard A , Citti C
Ref : BMC Genomics , 11 :86 , 2010
Abstract : BACKGROUND: While the genomic era is accumulating a tremendous amount of data, the question of how genomics can describe a bacterial species remains to be fully addressed. The recent sequencing of the genome of the Mycoplasma agalactiae type strain has challenged our general view on mycoplasmas by suggesting that these simple bacteria are able to exchange significant amount of genetic material via horizontal gene transfer. Yet, events that are shaping mycoplasma genomes and that are underlining diversity within this species have to be fully evaluated. For this purpose, we compared two strains that are representative of the genetic spectrum encountered in this species: the type strain PG2 which genome is already available and a field strain, 5632, which was fully sequenced and annotated in this study.
RESULTS: The two genomes differ by ca. 130 kbp with that of 5632 being the largest (1006 kbp). The make up of this additional genetic material mainly corresponds (i) to mobile genetic elements and (ii) to expanded repertoire of gene families that encode putative surface proteins and display features of highly-variable systems. More specifically, three entire copies of a previously described integrative conjugative element are found in 5632 that accounts for ca. 80 kbp. Other mobile genetic elements, found in 5632 but not in PG2, are the more classical insertion sequences which are related to those found in two other ruminant pathogens, M. bovis and M. mycoides subsp. mycoides SC. In 5632, repertoires of gene families encoding surface proteins are larger due to gene duplication. Comparative proteomic analyses of the two strains indicate that the additional coding capacity of 5632 affects the overall architecture of the surface and suggests the occurrence of new phase variable systems based on single nucleotide polymorphisms. CONCLUSION: Overall, comparative analyses of two M. agalactiae strains revealed a very dynamic genome which structure has been shaped by gene flow among ruminant mycoplasmas and expansion-reduction of gene repertoires encoding surface proteins, the expression of which is driven by localized genetic micro-events.
ESTHER : Nouvel_2010_BMC.Genomics_11_86
PubMedSearch : Nouvel_2010_BMC.Genomics_11_86
PubMedID: 20122262
Gene_locus related to this paper: myca6-d3vqz4 , mycap-a5ixe1 , mycap-a5ixe2 , mycap-a5ixk0 , mycap-a5ixt9 , mycap-a5iy19 , mycap-a5iza3 , mycap-a5ixl0

Title : The complete genome of Propionibacterium freudenreichii CIRM-BIA1, a hardy actinobacterium with food and probiotic applications - Falentin_2010_PLoS.ONE_5_E11748
Author(s) : Falentin H , Deutsch SM , Jan G , Loux V , Thierry A , Parayre S , Maillard MB , Dherbecourt J , Cousin FJ , Jardin J , Siguier P , Couloux A , Barbe V , Vacherie B , Wincker P , Gibrat JF , Gaillardin C , Lortal S
Ref : PLoS ONE , 5 :e11748 , 2010
Abstract : BACKGROUND: Propionibacterium freudenreichii is essential as a ripening culture in Swiss-type cheeses and is also considered for its probiotic use. This species exhibits slow growth, low nutritional requirements, and hardiness in many habitats. It belongs to the taxonomic group of dairy propionibacteria, in contrast to the cutaneous species P. acnes. The genome of the type strain, P. freudenreichii subsp. shermanii CIRM-BIA1 (CIP 103027(T)), was sequenced with an 11-fold coverage. METHODOLOGY/PRINCIPAL FINDINGS: The circular chromosome of 2.7 Mb of the CIRM-BIA1 strain has a GC-content of 67% and contains 22 different insertion sequences (3.5% of the genome in base pairs). Using a proteomic approach, 490 of the 2439 predicted proteins were confirmed. The annotation revealed the genetic basis for the hardiness of P. freudenreichii, as the bacterium possesses a complete enzymatic arsenal for de novo biosynthesis of aminoacids and vitamins (except panthotenate and biotin) as well as sequences involved in metabolism of various carbon sources, immunity against phages, duplicated chaperone genes and, interestingly, genes involved in the management of polyphosphate, glycogen and trehalose storage. The complete biosynthesis pathway for a bifidogenic compound is described, as well as a high number of surface proteins involved in interactions with the host and present in other probiotic bacteria. By comparative genomics, no pathogenicity factors found in P. acnes or in other pathogenic microbial species were identified in P. freudenreichii, which is consistent with the Generally Recognized As Safe and Qualified Presumption of Safety status of P. freudenreichii. Various pathways for formation of cheese flavor compounds were identified: the Wood-Werkman cycle for propionic acid formation, amino acid degradation pathways resulting in the formation of volatile branched chain fatty acids, and esterases involved in the formation of free fatty acids and esters. CONCLUSIONS/SIGNIFICANCE: With the exception of its ability to degrade lactose, P. freudenreichii seems poorly adapted to dairy niches. This genome annotation opens up new prospects for the understanding of the P. freudenreichii probiotic activity.
ESTHER : Falentin_2010_PLoS.ONE_5_E11748
PubMedSearch : Falentin_2010_PLoS.ONE_5_E11748
PubMedID: 20668525
Gene_locus related to this paper: profr-b3cky9 , profr-b3ckz1 , profr-b3ckz0 , profr-b3cky3

Title : Complete genome sequence of Crohn's disease-associated adherent-invasive E. coli strain LF82 - Miquel_2010_PLoS.One_5_e12714
Author(s) : Miquel S , Peyretaillade E , Claret L , de Vallee A , Dossat C , Vacherie B , Zineb el H , Segurens B , Barbe V , Sauvanet P , Neut C , Colombel JF , Medigue C , Mojica FJ , Peyret P , Bonnet R , Darfeuille-Michaud A
Ref : PLoS ONE , 5 : , 2010
Abstract : BACKGROUND: Ileal lesions of Crohn's disease (CD) patients are abnormally colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to invade and to replicate within intestinal epithelial cells and macrophages. PRINCIPAL FINDINGS: We report here the complete genome sequence of E. coli LF82, the reference strain of adherent-invasive E. coli associated with ileal Crohn's disease. The LF82 genome of 4,881,487 bp total size contains a circular chromosome with a size of 4,773,108 bp and a plasmid of 108,379 bp. The analysis of predicted coding sequences (CDSs) within the LF82 flexible genome indicated that this genome is close to the avian pathogenic strain APEC_01, meningitis-associated strain S88 and urinary-isolated strain UTI89 with regards to flexible genome and single nucleotide polymorphisms in various virulence factors. Interestingly, we observed that strains LF82 and UTI89 adhered at a similar level to Intestine-407 cells and that like LF82, APEC_01 and UTI89 were highly invasive. However, A1EC strain LF82 had an intermediate killer phenotype compared to APEC-01 and UTI89 and the LF82 genome does not harbour most of specific virulence genes from ExPEC. LF82 genome has evolved from those of ExPEC B2 strains by the acquisition of Salmonella and Yersinia isolated or clustered genes or CDSs located on pLF82 plasmid and at various loci on the chromosome. CONCLUSION: LF82 genome analysis indicated that a number of genes, gene clusters and pathoadaptative mutations which have been acquired may play a role in virulence of AIEC strain LF82.
ESTHER : Miquel_2010_PLoS.One_5_e12714
PubMedSearch : Miquel_2010_PLoS.One_5_e12714
PubMedID: 20862302
Gene_locus related to this paper: ecoli-yeiG , ecoli-yqia

Title : Genome sequences of Escherichia coli B strains REL606 and BL21(DE3) - Jeong_2009_J.Mol.Biol_394_644
Author(s) : Jeong H , Barbe V , Lee CH , Vallenet D , Yu DS , Choi SH , Couloux A , Lee SW , Yoon SH , Cattolico L , Hur CG , Park HS , Segurens B , Kim SC , Oh TK , Lenski RE , Studier FW , Daegelen P , Kim JF
Ref : Journal of Molecular Biology , 394 :644 , 2009
Abstract : Escherichia coli K-12 and B have been the subjects of classical experiments from which much of our understanding of molecular genetics has emerged. We present here complete genome sequences of two E. coli B strains, REL606, used in a long-term evolution experiment, and BL21(DE3), widely used to express recombinant proteins. The two genomes differ in length by 72,304 bp and have 426 single base pair differences, a seemingly large difference for laboratory strains having a common ancestor within the last 67 years. Transpositions by IS1 and IS150 have occurred in both lineages. Integration of the DE3 prophage in BL21(DE3) apparently displaced a defective prophage in the lambda attachment site of B. As might have been anticipated from the many genetic and biochemical experiments comparing B and K-12 over the years, the B genomes are similar in size and organization to the genome of E. coli K-12 MG1655 and have >99% sequence identity over approximately 92% of their genomes. E. coli B and K-12 differ considerably in distribution of IS elements and in location and composition of larger mobile elements. An unexpected difference is the absence of a large cluster of flagella genes in B, due to a 41 kbp IS1-mediated deletion. Gene clusters that specify the LPS core, O antigen, and restriction enzymes differ substantially, presumably because of horizontal transfer. Comparative analysis of 32 independently isolated E. coli and Shigella genomes, both commensals and pathogenic strains, identifies a minimal set of genes in common plus many strain-specific genes that constitute a large E. coli pan-genome.
ESTHER : Jeong_2009_J.Mol.Biol_394_644
PubMedSearch : Jeong_2009_J.Mol.Biol_394_644
PubMedID: 19786035
Gene_locus related to this paper: eco57-b3a913 , ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yiel , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-yqia , ecoli-YfhR

Title : From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later - Barbe_2009_Microbiology_155_1758
Author(s) : Barbe V , Cruveiller S , Kunst F , Lenoble P , Meurice G , Sekowska A , Vallenet D , Wang T , Moszer I , Medigue C , Danchin A
Ref : Microbiology , 155 :1758 , 2009
Abstract : Comparative genomics is the cornerstone of identification of gene functions. The immense number of living organisms precludes experimental identification of functions except in a handful of model organisms. The bacterial domain is split into large branches, among which the Firmicutes occupy a considerable space. Bacillus subtilis has been the model of Firmicutes for decades and its genome has been a reference for more than 10 years. Sequencing the genome involved more than 30 laboratories, with different expertises, in a attempt to make the most of the experimental information that could be associated with the sequence. This had the expected drawback that the sequencing expertise was quite varied among the groups involved, especially at a time when sequencing genomes was extremely hard work. The recent development of very efficient, fast and accurate sequencing techniques, in parallel with the development of high-level annotation platforms, motivated the present resequencing work. The updated sequence has been reannotated in agreement with the UniProt protein knowledge base, keeping in perspective the split between the paleome (genes necessary for sustaining and perpetuating life) and the cenome (genes required for occupation of a niche, suggesting here that B. subtilis is an epiphyte). This should permit investigators to make reliable inferences to prepare validation experiments in a variety of domains of bacterial growth and development as well as build up accurate phylogenies.
ESTHER : Barbe_2009_Microbiology_155_1758
PubMedSearch : Barbe_2009_Microbiology_155_1758
PubMedID: 19383706

Title : Non mycobacterial virulence genes in the genome of the emerging pathogen Mycobacterium abscessus - Ripoll_2009_PLoS.One_4_e5660
Author(s) : Ripoll F , Pasek S , Schenowitz C , Dossat C , Barbe V , Rottman M , Macheras E , Heym B , Herrmann JL , Daffe M , Brosch R , Risler JL , Gaillard JL
Ref : PLoS ONE , 4 :e5660 , 2009
Abstract : Mycobacterium abscessus is an emerging rapidly growing mycobacterium (RGM) causing a pseudotuberculous lung disease to which patients with cystic fibrosis (CF) are particularly susceptible. We report here its complete genome sequence. The genome of M. abscessus (CIP 104536T) consists of a 5,067,172-bp circular chromosome including 4920 predicted coding sequences (CDS), an 81-kb full-length prophage and 5 IS elements, and a 23-kb mercury resistance plasmid almost identical to pMM23 from Mycobacterium marinum. The chromosome encodes many virulence proteins and virulence protein families absent or present in only small numbers in the model RGM species Mycobacterium smegmatis. Many of these proteins are encoded by genes belonging to a "mycobacterial" gene pool (e.g. PE and PPE proteins, MCE and YrbE proteins, lipoprotein LpqH precursors). However, many others (e.g. phospholipase C, MgtC, MsrA, ABC Fe(3+) transporter) appear to have been horizontally acquired from distantly related environmental bacteria with a high G+C content, mostly actinobacteria (e.g. Rhodococcus sp., Streptomyces sp.) and pseudomonads. We also identified several metabolic regions acquired from actinobacteria and pseudomonads (relating to phenazine biosynthesis, homogentisate catabolism, phenylacetic acid degradation, DNA degradation) not present in the M. smegmatis genome. Many of the "non mycobacterial" factors detected in M. abscessus are also present in two of the pathogens most frequently isolated from CF patients, Pseudomonas aeruginosa and Burkholderia cepacia. This study elucidates the genetic basis of the unique pathogenicity of M. abscessus among RGM, and raises the question of similar mechanisms of pathogenicity shared by unrelated organisms in CF patients.
ESTHER : Ripoll_2009_PLoS.One_4_e5660
PubMedSearch : Ripoll_2009_PLoS.One_4_e5660
PubMedID: 19543527

Title : Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths - Touchon_2009_PLoS.Genet_5_e1000344
Author(s) : Touchon M , Hoede C , Tenaillon O , Barbe V , Baeriswyl S , Bidet P , Bingen E , Bonacorsi S , Bouchier C , Bouvet O , Calteau A , Chiapello H , Clermont O , Cruveiller S , Danchin A , Diard M , Dossat C , Karoui ME , Frapy E , Garry L , Ghigo JM , Gilles AM , Johnson J , Le Bouguenec C , Lescat M , Mangenot S , Martinez-Jehanne V , Matic I , Nassif X , Oztas S , Petit MA , Pichon C , Rouy Z , Ruf CS , Schneider D , Tourret J , Vacherie B , Vallenet D , Medigue C , Rocha EP , Denamur E
Ref : PLoS Genet , 5 :e1000344 , 2009
Abstract : The Escherichia coli species represents one of the best-studied model organisms, but also encompasses a variety of commensal and pathogenic strains that diversify by high rates of genetic change. We uniformly (re-) annotated the genomes of 20 commensal and pathogenic E. coli strains and one strain of E. fergusonii (the closest E. coli related species), including seven that we sequenced to completion. Within the approximately 18,000 families of orthologous genes, we found approximately 2,000 common to all strains. Although recombination rates are much higher than mutation rates, we show, both theoretically and using phylogenetic inference, that this does not obscure the phylogenetic signal, which places the B2 phylogenetic group and one group D strain at the basal position. Based on this phylogeny, we inferred past evolutionary events of gain and loss of genes, identifying functional classes under opposite selection pressures. We found an important adaptive role for metabolism diversification within group B2 and Shigella strains, but identified few or no extraintestinal virulence-specific genes, which could render difficult the development of a vaccine against extraintestinal infections. Genome flux in E. coli is confined to a small number of conserved positions in the chromosome, which most often are not associated with integrases or tRNA genes. Core genes flanking some of these regions show higher rates of recombination, suggesting that a gene, once acquired by a strain, spreads within the species by homologous recombination at the flanking genes. Finally, the genome's long-scale structure of recombination indicates lower recombination rates, but not higher mutation rates, at the terminus of replication. The ensuing effect of background selection and biased gene conversion may thus explain why this region is A+T-rich and shows high sequence divergence but low sequence polymorphism. Overall, despite a very high gene flow, genes co-exist in an organised genome.
ESTHER : Touchon_2009_PLoS.Genet_5_e1000344
PubMedSearch : Touchon_2009_PLoS.Genet_5_e1000344
PubMedID: 19165319
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C0410 , ecoli-C2429 , ecoli-C3633 , ecoli-C3636 , ecoli-C4836 , ecoli-d7xp23 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-IROD , ecoli-IROE , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yiel , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-ypt1 , ecoli-yqia , ecoli-Z0347 , ecoli-Z1930 , ecoli-YfhR , ecout-q1r7l6 , escfe-e9z855 , yerpe-YBTT , ecolx-e0qx45

Title : Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas - Pereyre_2009_PLoS.Genet_5_e1000677
Author(s) : Pereyre S , Sirand-Pugnet P , Beven L , Charron A , Renaudin H , Barre A , Avenaud P , Jacob D , Couloux A , Barbe V , de Daruvar A , Blanchard A , Bebear C
Ref : PLoS Genet , 5 :e1000677 , 2009
Abstract : Mycoplasma hominis is an opportunistic human mycoplasma. Two other pathogenic human species, M. genitalium and Ureaplasma parvum, reside within the same natural niche as M. hominis: the urogenital tract. These three species have overlapping, but distinct, pathogenic roles. They have minimal genomes and, thus, reduced metabolic capabilities characterized by distinct energy-generating pathways. Analysis of the M. hominis PG21 genome sequence revealed that it is the second smallest genome among self-replicating free living organisms (665,445 bp, 537 coding sequences (CDSs)). Five clusters of genes were predicted to have undergone horizontal gene transfer (HGT) between M. hominis and the phylogenetically distant U. parvum species. We reconstructed M. hominis metabolic pathways from the predicted genes, with particular emphasis on energy-generating pathways. The Embden-Meyerhoff-Parnas pathway was incomplete, with a single enzyme absent. We identified the three proteins constituting the arginine dihydrolase pathway. This pathway was found essential to promote growth in vivo. The predicted presence of dimethylarginine dimethylaminohydrolase suggested that arginine catabolism is more complex than initially described. This enzyme may have been acquired by HGT from non-mollicute bacteria. Comparison of the three minimal mollicute genomes showed that 247 CDSs were common to all three genomes, whereas 220 CDSs were specific to M. hominis, 172 CDSs were specific to M. genitalium, and 280 CDSs were specific to U. parvum. Within these species-specific genes, two major sets of genes could be identified: one including genes involved in various energy-generating pathways, depending on the energy source used (glucose, urea, or arginine) and another involved in cytadherence and virulence. Therefore, a minimal mycoplasma cell, not including cytadherence and virulence-related genes, could be envisaged containing a core genome (247 genes), plus a set of genes required for providing energy. For M. hominis, this set would include 247+9 genes, resulting in a theoretical minimal genome of 256 genes.
ESTHER : Pereyre_2009_PLoS.Genet_5_e1000677
PubMedSearch : Pereyre_2009_PLoS.Genet_5_e1000677
PubMedID: 19816563
Gene_locus related to this paper: mychp-d1j8v6

Title : The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae - Pieretti_2009_BMC.Genomics_10_616
Author(s) : Pieretti I , Royer M , Barbe V , Carrere S , Koebnik R , Cociancich S , Couloux A , Darrasse A , Gouzy J , Jacques MA , Lauber E , Manceau C , Mangenot S , Poussier S , Segurens B , Szurek B , Verdier V , Arlat M , Rott P
Ref : BMC Genomics , 10 :616 , 2009
Abstract : BACKGROUND: The Xanthomonadaceae family contains two xylem-limited plant pathogenic bacterial species, Xanthomonas albilineans and Xylella fastidiosa. X. fastidiosa was the first completely sequenced plant pathogen. It is insect-vectored, has a reduced genome and does not possess hrp genes which encode a Type III secretion system found in most plant pathogenic bacteria. X. fastidiosa was excluded from the Xanthomonas group based on phylogenetic analyses with rRNA sequences.
RESULTS: The complete genome of X. albilineans was sequenced and annotated. X. albilineans, which is not known to be insect-vectored, also has a reduced genome and does not possess hrp genes. Phylogenetic analysis using X. albilineans genomic sequences showed that X. fastidiosa belongs to the Xanthomonas group. Order of divergence of the Xanthomonadaceae revealed that X. albilineans and X. fastidiosa experienced a convergent reductive genome evolution during their descent from the progenitor of the Xanthomonas genus. Reductive genome evolutions of the two xylem-limited Xanthomonadaceae were compared in light of their genome characteristics and those of obligate animal symbionts and pathogens. CONCLUSION: The two xylem-limited Xanthomonadaceae, during their descent from a common ancestral parent, experienced a convergent reductive genome evolution. Adaptation to the nutrient-poor xylem elements and to the cloistered environmental niche of xylem vessels probably favoured this convergent evolution. However, genome characteristics of X. albilineans differ from those of X. fastidiosa and obligate animal symbionts and pathogens, indicating that a distinctive process was responsible for the reductive genome evolution in this pathogen. The possible role in genome reduction of the unique toxin albicidin, produced by X. albilineans, is discussed.
ESTHER : Pieretti_2009_BMC.Genomics_10_616
PubMedSearch : Pieretti_2009_BMC.Genomics_10_616
PubMedID: 20017926
Gene_locus related to this paper: xanal-q53b17 , xanap-d2u924 , xanap-d2uar7 , xanap-d2ub15 , xanap-d2ucz4 , xanap-d2ue41 , xanap-d2ue72 , xanap-d2ueb5 , xanap-d2uf40 , xanap-d2ugg6 , xanap-d2ugj3 , xanap-d2ugr3 , xanap-d2ugv1 , xanap-d2ubh2 , xanap-d2ud71 , xanap-d2u9n1 , xanap-d2uci9

Title : Comparative genomics of protoploid Saccharomycetaceae - Souciet_2009_Genome.Res_19_1696
Author(s) : Souciet JL , Dujon B , Gaillardin C , Johnston M , Baret PV , Cliften P , Sherman DJ , Weissenbach J , Westhof E , Wincker P , Jubin C , Poulain J , Barbe V , Segurens B , Artiguenave F , Anthouard V , Vacherie B , Val ME , Fulton RS , Minx P , Wilson R , Durrens P , Jean G , Marck C , Martin T , Nikolski M , Rolland T , Seret ML , Casaregola S , Despons L , Fairhead C , Fischer G , Lafontaine I , Leh V , Lemaire M , De Montigny J , Neuveglise C , Thierry A , Blanc-Lenfle I , Bleykasten C , Diffels J , Fritsch E , Frangeul L , Goeffon A , Jauniaux N , Kachouri-Lafond R , Payen C , Potier S , Pribylova L , Ozanne C , Richard GF , Sacerdot C , Straub ML , Talla E
Ref : Genome Res , 19 :1696 , 2009
Abstract : Our knowledge of yeast genomes remains largely dominated by the extensive studies on Saccharomyces cerevisiae and the consequences of its ancestral duplication, leaving the evolution of the entire class of hemiascomycetes only partly explored. We concentrate here on five species of Saccharomycetaceae, a large subdivision of hemiascomycetes, that we call "protoploid" because they diverged from the S. cerevisiae lineage prior to its genome duplication. We determined the complete genome sequences of three of these species: Kluyveromyces (Lachancea) thermotolerans and Saccharomyces (Lachancea) kluyveri (two members of the newly described Lachancea clade), and Zygosaccharomyces rouxii. We included in our comparisons the previously available sequences of Kluyveromyces lactis and Ashbya (Eremothecium) gossypii. Despite their broad evolutionary range and significant individual variations in each lineage, the five protoploid Saccharomycetaceae share a core repertoire of approximately 3300 protein families and a high degree of conserved synteny. Synteny blocks were used to define gene orthology and to infer ancestors. Far from representing minimal genomes without redundancy, the five protoploid yeasts contain numerous copies of paralogous genes, either dispersed or in tandem arrays, that, altogether, constitute a third of each genome. Ancient, conserved paralogs as well as novel, lineage-specific paralogs were identified.
ESTHER : Souciet_2009_Genome.Res_19_1696
PubMedSearch : Souciet_2009_Genome.Res_19_1696
PubMedID: 19525356
Gene_locus related to this paper: lactc-c5dci9 , lactc-c5ddi5 , lactc-c5dew5 , lactc-c5dez1 , lactc-c5df11 , lactc-c5dfh7 , lactc-c5dgd1 , lactc-c5dif7 , lactc-c5din7 , lactc-c5dja0 , lactc-c5dm95 , lactc-c5dn06 , lactc-c5dnn9 , lactc-c5e2g8 , lactc-c5e3n5 , lactc-c5e375 , zygrc-c5drr0 , zygrc-c5dvh0 , zygrc-c5dvl2 , zygrc-c5dvx0 , zygrc-c5dvz8 , zygrc-c5dx83 , zygrc-c5dxn5 , zygrc-c5dxq9 , zygrc-c5e0w1 , zygrc-c5e1e4 , zygrc-c5e1h2 , zygro-a0a1q2zt01 , 9sach-a0a0p1kuu1 , lactc-kex1 , zygrc-kex1

Title : Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti - de Groot_2009_PLoS.Genet_5_e1000434
Author(s) : de Groot A , Dulermo R , Ortet P , Blanchard L , Guerin P , Fernandez B , Vacherie B , Dossat C , Jolivet E , Siguier P , Chandler M , Barakat M , Dedieu A , Barbe V , Heulin T , Sommer S , Achouak W , Armengaud J
Ref : PLoS Genet , 5 :e1000434 , 2009
Abstract : To better understand adaptation to harsh conditions encountered in hot arid deserts, we report the first complete genome sequence and proteome analysis of a bacterium, Deinococcus deserti VCD115, isolated from Sahara surface sand. Its genome consists of a 2.8-Mb chromosome and three large plasmids of 324 kb, 314 kb, and 396 kb. Accurate primary genome annotation of its 3,455 genes was guided by extensive proteome shotgun analysis. From the large corpus of MS/MS spectra recorded, 1,348 proteins were uncovered and semiquantified by spectral counting. Among the highly detected proteins are several orphans and Deinococcus-specific proteins of unknown function. The alliance of proteomics and genomics high-throughput techniques allowed identification of 15 unpredicted genes and, surprisingly, reversal of incorrectly predicted orientation of 11 genes. Reversal of orientation of two Deinococcus-specific radiation-induced genes, ddrC and ddrH, and identification in D. deserti of supplementary genes involved in manganese import extend our knowledge of the radiotolerance toolbox of Deinococcaceae. Additional genes involved in nutrient import and in DNA repair (i.e., two extra recA, three translesion DNA polymerases, a photolyase) were also identified and found to be expressed under standard growth conditions, and, for these DNA repair genes, after exposure of the cells to UV. The supplementary nutrient import and DNA repair genes are likely important for survival and adaptation of D. deserti to its nutrient-poor, dry, and UV-exposed extreme environment.
ESTHER : de Groot_2009_PLoS.Genet_5_e1000434
PubMedSearch : de Groot_2009_PLoS.Genet_5_e1000434
PubMedID: 19370165
Gene_locus related to this paper: deidv-c1cva5 , deidv-c1cvi6 , deidv-c1cwe6 , deidv-c1cwk6 , deidv-c1cxm7 , deidv-c1cy72 , deidv-c1d002 , deidv-c1d2d8 , deidv-c1d3d8 , deidv-c1d3p0 , deidv-c1cxw0 , deidv-c1d2x1 , deidv-c1cvt7

Title : Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources - Vuilleumier_2009_PLoS.One_4_e5584
Author(s) : Vuilleumier S , Chistoserdova L , Lee MC , Bringel F , Lajus A , Zhou Y , Gourion B , Barbe V , Chang J , Cruveiller S , Dossat C , Gillett W , Gruffaz C , Haugen E , Hourcade E , Levy R , Mangenot S , Muller E , Nadalig T , Pagni M , Penny C , Peyraud R , Robinson DG , Roche D , Rouy Z , Saenampechek C , Salvignol G , Vallenet D , Wu Z , Marx CJ , Vorholt JA , Olson MV , Kaul R , Weissenbach J , Medigue C , Lidstrom ME
Ref : PLoS ONE , 4 :e5584 , 2009
Abstract : BACKGROUND: Methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. The genomes of two pink-pigmented facultative methylotrophic bacteria of the Alpha-proteobacterial genus Methylobacterium, the reference species Methylobacterium extorquens strain AM1 and the dichloromethane-degrading strain DM4, were compared. METHODOLOGY/PRINCIPAL FINDINGS: The 6.88 Mb genome of strain AM1 comprises a 5.51 Mb chromosome, a 1.26 Mb megaplasmid and three plasmids, while the 6.12 Mb genome of strain DM4 features a 5.94 Mb chromosome and two plasmids. The chromosomes are highly syntenic and share a large majority of genes, while plasmids are mostly strain-specific, with the exception of a 130 kb region of the strain AM1 megaplasmid which is syntenic to a chromosomal region of strain DM4. Both genomes contain large sets of insertion elements, many of them strain-specific, suggesting an important potential for genomic plasticity. Most of the genomic determinants associated with methylotrophy are nearly identical, with two exceptions that illustrate the metabolic and genomic versatility of Methylobacterium. A 126 kb dichloromethane utilization (dcm) gene cluster is essential for the ability of strain DM4 to use DCM as the sole carbon and energy source for growth and is unique to strain DM4. The methylamine utilization (mau) gene cluster is only found in strain AM1, indicating that strain DM4 employs an alternative system for growth with methylamine. The dcm and mau clusters represent two of the chromosomal genomic islands (AM1: 28; DM4: 17) that were defined. The mau cluster is flanked by mobile elements, but the dcm cluster disrupts a gene annotated as chelatase and for which we propose the name "island integration determinant" (iid). CONCLUSION/SIGNIFICANCE: These two genome sequences provide a platform for intra- and interspecies genomic comparisons in the genus Methylobacterium, and for investigations of the adaptive mechanisms which allow bacterial lineages to acquire methylotrophic lifestyles.
ESTHER : Vuilleumier_2009_PLoS.One_4_e5584
PubMedSearch : Vuilleumier_2009_PLoS.One_4_e5584
PubMedID: 19440302
Gene_locus related to this paper: metc4-b7krz1 , metea-c5asz7 , metea-c5au09 , metea-c5axg7 , metea-c5b1t3 , metea-c5b215 , metea-c5b387 , meted-c7cbs2 , meted-c7ce76 , meted-c7cfe3 , meted-c7cfx5 , meted-c7cg08 , meted-c7cgc9 , meted-c7cge7 , meted-c7chb8 , meted-c7ci36 , meted-c7cln3 , meted-c7cnd9 , metep-a9vxp1 , metep-a9w2b1 , metep-a9w028 , metex-orf5 , metex-Q8RPA1 , metpb-b1zjw5 , metea-c5as87 , metea-c5awv9 , meted-c7cb08 , metea-rutd , meted-rutd

Title : Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia - Amadou_2008_Genome.Res_18_1472
Author(s) : Amadou C , Pascal G , Mangenot S , Glew M , Bontemps C , Capela D , Carrere S , Cruveiller S , Dossat C , Lajus A , Marchetti M , Poinsot V , Rouy Z , Servin B , Saad M , Schenowitz C , Barbe V , Batut J , Medigue C , Masson-Boivin C
Ref : Genome Res , 18 :1472 , 2008
Abstract : We report the first complete genome sequence of a beta-proteobacterial nitrogen-fixing symbiont of legumes, Cupriavidus taiwanensis LMG19424. The genome consists of two chromosomes of size 3.42 Mb and 2.50 Mb, and a large symbiotic plasmid of 0.56 Mb. The C. taiwanensis genome displays an unexpected high similarity with the genome of the saprophytic bacterium C. eutrophus H16, despite being 0.94 Mb smaller. Both organisms harbor two chromosomes with large regions of synteny interspersed by specific regions. In contrast, the two species host highly divergent plasmids, with the consequence that C. taiwanensis is symbiotically proficient and less metabolically versatile. Altogether, specific regions in C. taiwanensis compared with C. eutrophus cover 1.02 Mb and are enriched in genes associated with symbiosis or virulence in other bacteria. C. taiwanensis reveals characteristics of a minimal rhizobium, including the most compact (35-kb) symbiotic island (nod and nif) identified so far in any rhizobium. The atypical phylogenetic position of C. taiwanensis allowed insightful comparative genomics of all available rhizobium genomes. We did not find any gene that was both common and specific to all rhizobia, thus suggesting that a unique shared genetic strategy does not support symbiosis of rhizobia with legumes. Instead, phylodistribution analysis of more than 200 Sinorhizobium meliloti known symbiotic genes indicated large and complex variations of their occurrence in rhizobia and non-rhizobia. This led us to devise an in silico method to extract genes preferentially associated with rhizobia. We discuss how the novel genes we have identified may contribute to symbiotic adaptation.
ESTHER : Amadou_2008_Genome.Res_18_1472
PubMedSearch : Amadou_2008_Genome.Res_18_1472
PubMedID: 18490699
Gene_locus related to this paper: cuppj-metx , cuppj-q46nh7 , cuptr-b2agb4 , cuptr-b2ahd0 , cuptr-b2ahw1 , cuptr-b2ai18 , cuptr-b2ai31 , cuptr-b2aii9 , cuptr-b2aik0 , cuptr-b2aiq3 , cuptr-b3r2j1 , cuptr-b3r3y6 , cuptr-b3r4w6 , cuptr-b3r7p4 , cuptr-b3r8d6 , cuptr-b3r8f5 , cuptr-b3r9z0 , cuptr-b3r255 , cuptr-b3r457 , cuptr-b3r543 , cuptr-b3ras4 , cuptr-b3rau3 , cuptr-b3rb04 , cuptr-b3rbm8 , cuptr-b3rd43 , cuptr-b3r6t6 , cuptr-b3r3f3 , cuptr-b3r2m0

Title : Comparative analysis of Acinetobacters: three genomes for three lifestyles - Vallenet_2008_PLoS.One_3_e1805
Author(s) : Vallenet D , Nordmann P , Barbe V , Poirel L , Mangenot S , Bataille E , Dossat C , Gas S , Kreimeyer A , Lenoble P , Oztas S , Poulain J , Segurens B , Robert C , Abergel C , Claverie JM , Raoult D , Medigue C , Weissenbach J , Cruveiller S
Ref : PLoS ONE , 3 :e1805 , 2008
Abstract : Acinetobacter baumannii is the source of numerous nosocomial infections in humans and therefore deserves close attention as multidrug or even pandrug resistant strains are increasingly being identified worldwide. Here we report the comparison of two newly sequenced genomes of A. baumannii. The human isolate A. baumannii AYE is multidrug resistant whereas strain SDF, which was isolated from body lice, is antibiotic susceptible. As reference for comparison in this analysis, the genome of the soil-living bacterium A. baylyi strain ADP1 was used. The most interesting dissimilarities we observed were that i) whereas strain AYE and A. baylyi genomes harbored very few Insertion Sequence elements which could promote expression of downstream genes, strain SDF sequence contains several hundred of them that have played a crucial role in its genome reduction (gene disruptions and simple DNA loss); ii) strain SDF has low catabolic capacities compared to strain AYE. Interestingly, the latter has even higher catabolic capacities than A. baylyi which has already been reported as a very nutritionally versatile organism. This metabolic performance could explain the persistence of A. baumannii nosocomial strains in environments where nutrients are scarce; iii) several processes known to play a key role during host infection (biofilm formation, iron uptake, quorum sensing, virulence factors) were either different or absent, the best example of which is iron uptake. Indeed, strain AYE and A. baylyi use siderophore-based systems to scavenge iron from the environment whereas strain SDF uses an alternate system similar to the Haem Acquisition System (HAS). Taken together, all these observations suggest that the genome contents of the 3 Acinetobacters compared are partly shaped by life in distinct ecological niches: human (and more largely hospital environment), louse, soil.
ESTHER : Vallenet_2008_PLoS.One_3_e1805
PubMedSearch : Vallenet_2008_PLoS.One_3_e1805
PubMedID: 18350144
Gene_locus related to this paper: acib1-e8pgf8 , acib3-b7guy6 , acib3-b7h156 , acib3-metx , aciba-d0c992 , aciba-k1epl1 , aciba-k6lkl9 , acibc-b2huf4 , acibc-b2i0a2 , acibc-b2i0w9 , acibc-b2i2b0 , acibs-b0vt32 , acibt-a3m1g6 , acibt-a3m5r6 , acibt-a3m5t3 , acibt-a3m5x2 , acibt-a3m627 , acibt-a3m707 , aciby-b0v723 , acica-d0s0a7 , aciju-d0sj67 , aciba-f5iht4 , aciba-a0a009wzt4

Title : A tale of two oxidation states: bacterial colonization of arsenic-rich environments - Muller_2007_PLoS.Genet_3_e53
Author(s) : Muller D , Medigue C , Koechler S , Barbe V , Barakat M , Talla E , Bonnefoy V , Krin E , Arsene-Ploetze F , Carapito C , Chandler M , Cournoyer B , Cruveiller S , Dossat C , Duval S , Heymann M , Leize E , Lieutaud A , Lievremont D , Makita Y , Mangenot S , Nitschke W , Ortet P , Perdrial N , Schoepp B , Siguier P , Simeonova DD , Rouy Z , Segurens B , Turlin E , Vallenet D , Van Dorsselaer A , Weiss S , Weissenbach J , Lett MC , Danchin A , Bertin PN
Ref : PLoS Genet , 3 :e53 , 2007
Abstract : Microbial biotransformations have a major impact on contamination by toxic elements, which threatens public health in developing and industrial countries. Finding a means of preserving natural environments-including ground and surface waters-from arsenic constitutes a major challenge facing modern society. Although this metalloid is ubiquitous on Earth, thus far no bacterium thriving in arsenic-contaminated environments has been fully characterized. In-depth exploration of the genome of the beta-proteobacterium Herminiimonas arsenicoxydans with regard to physiology, genetics, and proteomics, revealed that it possesses heretofore unsuspected mechanisms for coping with arsenic. Aside from multiple biochemical processes such as arsenic oxidation, reduction, and efflux, H. arsenicoxydans also exhibits positive chemotaxis and motility towards arsenic and metalloid scavenging by exopolysaccharides. These observations demonstrate the existence of a novel strategy to efficiently colonize arsenic-rich environments, which extends beyond oxidoreduction reactions. Such a microbial mechanism of detoxification, which is possibly exploitable for bioremediation applications of contaminated sites, may have played a crucial role in the occupation of ancient ecological niches on earth.
ESTHER : Muller_2007_PLoS.Genet_3_e53
PubMedSearch : Muller_2007_PLoS.Genet_3_e53
PubMedID: 17432936
Gene_locus related to this paper: herar-a4g4w8 , herar-a4g5p0 , herar-a4g6p3 , herar-a4g378 , herar-a4g411 , herar-a4g622 , herar-a4g818 , herar-a4g899 , herar-a4gac3 , herar-metx , herar-a4g8n7

Title : Being pathogenic, plastic, and sexual while living with a nearly minimal bacterial genome - Sirand-Pugnet_2007_PLoS.Genet_3_e75
Author(s) : Sirand-Pugnet P , Lartigue C , Marenda M , Jacob D , Barre A , Barbe V , Schenowitz C , Mangenot S , Couloux A , Segurens B , de Daruvar A , Blanchard A , Citti C
Ref : PLoS Genet , 3 :e75 , 2007
Abstract : Mycoplasmas are commonly described as the simplest self-replicating organisms, whose evolution was mainly characterized by genome downsizing with a proposed evolutionary scenario similar to that of obligate intracellular bacteria such as insect endosymbionts. Thus far, analysis of mycoplasma genomes indicates a low level of horizontal gene transfer (HGT) implying that DNA acquisition is strongly limited in these minimal bacteria. In this study, the genome of the ruminant pathogen Mycoplasma agalactiae was sequenced. Comparative genomic data and phylogenetic tree reconstruction revealed that approximately 18% of its small genome (877,438 bp) has undergone HGT with the phylogenetically distinct mycoides cluster, which is composed of significant ruminant pathogens. HGT involves genes often found as clusters, several of which encode lipoproteins that usually play an important role in mycoplasma-host interaction. A decayed form of a conjugative element also described in a member of the mycoides cluster was found in the M. agalactiae genome, suggesting that HGT may have occurred by mobilizing a related genetic element. The possibility of HGT events among other mycoplasmas was evaluated with the available sequenced genomes. Our data indicate marginal levels of HGT among Mycoplasma species except for those described above and, to a lesser extent, for those observed in between the two bird pathogens, M. gallisepticum and M. synoviae. This first description of large-scale HGT among mycoplasmas sharing the same ecological niche challenges the generally accepted evolutionary scenario in which gene loss is the main driving force of mycoplasma evolution. The latter clearly differs from that of other bacteria with small genomes, particularly obligate intracellular bacteria that are isolated within host cells. Consequently, mycoplasmas are not only able to subvert complex hosts but presumably have retained sexual competence, a trait that may prevent them from genome stasis and contribute to adaptation to new hosts.
ESTHER : Sirand-Pugnet_2007_PLoS.Genet_3_e75
PubMedSearch : Sirand-Pugnet_2007_PLoS.Genet_3_e75
PubMedID: 17511520
Gene_locus related to this paper: mycap-a5ixe1 , mycap-a5ixe2 , mycap-a5ixk0 , mycap-a5ixt9 , mycap-a5iy19 , mycap-a5iza3 , mycap-a5ixl0

Title : Legumes symbioses: absence of Nod genes in photosynthetic bradyrhizobia - Giraud_2007_Science_316_1307
Author(s) : Giraud E , Moulin L , Vallenet D , Barbe V , Cytryn E , Avarre JC , Jaubert M , Simon D , Cartieaux F , Prin Y , Bena G , Hannibal L , Fardoux J , Kojadinovic M , Vuillet L , Lajus A , Cruveiller S , Rouy Z , Mangenot S , Segurens B , Dossat C , Franck WL , Chang WS , Saunders E , Bruce D , Richardson P , Normand P , Dreyfus B , Pignol D , Stacey G , Emerich D , Vermeglio A , Medigue C , Sadowsky M
Ref : Science , 316 :1307 , 2007
Abstract : Leguminous plants (such as peas and soybeans) and rhizobial soil bacteria are symbiotic partners that communicate through molecular signaling pathways, resulting in the formation of nodules on legume roots and occasionally stems that house nitrogen-fixing bacteria. Nodule formation has been assumed to be exclusively initiated by the binding of bacterial, host-specific lipochito-oligosaccharidic Nod factors, encoded by the nodABC genes, to kinase-like receptors of the plant. Here we show by complete genome sequencing of two symbiotic, photosynthetic, Bradyrhizobium strains, BTAi1 and ORS278, that canonical nodABC genes and typical lipochito-oligosaccharidic Nod factors are not required for symbiosis in some legumes. Mutational analyses indicated that these unique rhizobia use an alternative pathway to initiate symbioses, where a purine derivative may play a key role in triggering nodule formation.
ESTHER : Giraud_2007_Science_316_1307
PubMedSearch : Giraud_2007_Science_316_1307
PubMedID: 17540897
Gene_locus related to this paper: brasb-a5e8s7 , brasb-a5e9h9 , brasb-a5e9x2 , brasb-a5eac3 , brasb-a5eb24 , brasb-a5ech6 , brasb-a5eck9 , brasb-a5ed44 , brasb-a5edz7 , brasb-a5ee62 , brasb-a5ees1 , brasb-a5ef53 , brasb-a5efp3 , brasb-a5efp4 , brasb-a5eg29 , brasb-a5eh09 , brasb-a5ei81 , brasb-a5eiy7 , brasb-a5ej26 , brasb-a5ek41 , brasb-a5elh0 , brasb-a5ema7 , brasb-a5emc8 , brasb-a5eml7 , brasb-a5ene5 , brasb-a5ent6 , brasb-a5eny8 , brasb-a5ep81 , brasb-a5eph8 , brasb-a5epv4 , brasb-a5epx9 , brasb-a5eqb3 , brasb-a5erc8 , brasb-a5esb1 , brasb-a5ese9 , brasb-a5esl7 , brasb-a5esv5 , brasb-a5esw6 , brasb-a5etk7 , brasb-a5eul1 , braso-a4yk16 , braso-a4yl66 , braso-a4ylm4 , braso-a4ylr9 , braso-a4ylx7 , braso-a4ymj8 , braso-a4ynl1 , braso-a4ypd9 , braso-a4yqh3 , braso-a4yr10 , braso-a4yri0 , braso-a4yt56 , braso-a4yul4 , braso-a4yw76 , braso-a4ywb6 , braso-a4yxg2 , braso-a4yy49 , braso-a4yyj6 , braso-a4yzd7 , braso-a4yzh0 , braso-a4z0q9 , braso-a4z0v7 , braso-a4z1h1 , braso-a4z1p1 , braso-a4z1p8 , braso-a4z1v6 , braso-a4z2a5 , braso-a4z152 , braso-a4yl32 , brasb-a5et63 , brasb-a5emr8 , braso-a4ynl2 , brasb-a5eqb2 , braso-a4yr63

Title : Evolution of the terminal regions of the Streptomyces linear chromosome - Choulet_2006_Mol.Biol.Evol_23_2361
Author(s) : Choulet F , Aigle B , Gallois A , Mangenot S , Gerbaud C , Truong C , Francou FX , Fourrier C , Guerineau M , Decaris B , Barbe V , Pernodet JL , Leblond P
Ref : Molecular Biology Evolution , 23 :2361 , 2006
Abstract : Comparative analysis of the Streptomyces chromosome sequences, between Streptomyces coelicolor, Streptomyces avermitilis, and Streptomyces ambofaciens ATCC23877 (whose partial sequence is released in this study), revealed a highly compartmentalized genetic organization of their genome. Indeed, despite the presence of specific genomic islands, the central part of the chromosome appears highly syntenic. In contrast, the chromosome of each species exhibits large species-specific terminal regions (from 753 to 1,393 kb), even when considering closely related species (S. ambofaciens and S. coelicolor). Interestingly, the size of the central conserved region between species decreases as the phylogenetic distance between them increases, whereas the specific terminal fraction reciprocally increases in size. Between highly syntenic central regions and species-specific chromosomal parts, there is a notable degeneration of synteny due to frequent insertions/deletions. This reveals a massive and constant genomic flux (from lateral gene transfer and DNA rearrangements) affecting the terminal contingency regions. We speculate that a gradient of recombination rate (i.e., insertion/deletion events) toward the extremities is the force driving the exclusion of essential genes from the terminal regions (i.e., chromosome compartmentalization) and generating a fast gene turnover for strong adaptation capabilities.
ESTHER : Choulet_2006_Mol.Biol.Evol_23_2361
PubMedSearch : Choulet_2006_Mol.Biol.Evol_23_2361
PubMedID: 16956972
Gene_locus related to this paper: stram-a0abw5 , stram-a0ac15 , stram-a0ac26 , stram-a0ac27 , stram-a0acp3 , stram-a0acq4 , stram-a0acx2.1 , stram-a0acx2.2 , stram-a0adx4 , stram-a0aeb0 , stram-a3ki49 , stram-a3ki77 , stram-a3ki84 , stram-a3ki91 , stram-a3kik7 , stram-a3kj27 , stram-a3kkf9 , stram-a3kkk4 , stram-a3kkk5 , stram-q1rrf2 , strco-SCF11.06 , strco-SCO7057 , stram-a0acr7

Title : Intraspecific variability of the terminal inverted repeats of the linear chromosome of Streptomyces ambofaciens - Choulet_2006_J.Bacteriol_188_6599
Author(s) : Choulet F , Gallois A , Aigle B , Mangenot S , Gerbaud C , Truong C , Francou FX , Borges F , Fourrier C , Guerineau M , Decaris B , Barbe V , Pernodet JL , Leblond P
Ref : Journal of Bacteriology , 188 :6599 , 2006
Abstract : The sequences of the terminal inverted repeats (TIRs) ending the linear chromosomal DNA of two Streptomyces ambofaciens strains, ATCC23877 and DSM40697 (198 kb and 213 kb, respectively), were determined from two sets of recombinant cosmids. Among the 215 coding DNA sequences (CDSs) predicted in the TIRs of strain DSM40697, 65 are absent in the TIRs of strain ATCC23877. Reciprocally, 45 of the 194 predicted CDSs are specific to the ATCC23877 strain. The strain-specific CDSs are located mainly at the terminal end of the TIRs. Indeed, although TIRs appear almost identical over 150 kb (99% nucleotide identity), large regions of DNA of 60 kb (DSM40697) and 48 kb (ATCC23877), mostly spanning the ends of the chromosome, are strain specific. These regions are rich in plasmid-associated genes, including genes encoding putative conjugal transfer functions. The strain-specific regions also share a G+C content (68%) lower than that of the rest of the genome (from 71% to 73%), a percentage that is more typical of Streptomyces plasmids and mobile elements. These data suggest that exchanges of replicon extremities have occurred, thereby contributing to the terminal variability observed at the intraspecific level. In addition, the terminal regions include many mobile genetic element-related genes, pseudogenes, and genes related to adaptation. The results give insight into the mechanisms of evolution of the TIRs: integration of new information and/or loss of DNA fragments and subsequent homogenization of the two chromosomal extremities.
ESTHER : Choulet_2006_J.Bacteriol_188_6599
PubMedSearch : Choulet_2006_J.Bacteriol_188_6599
PubMedID: 16952952
Gene_locus related to this paper: stram-q0jwm8 , stram-q1rql3 , stram-q1rqp7 , stram-q1rqu8 , stram-q1rqw8 , stram-q1rr14 , stram-q1rr40 , stram-q1rr62 , stram-q1rrc9 , stram-q1rrf2 , stram-Q9KWX3

Title : Deciphering the evolution and metabolism of an anammox bacterium from a community genome - Strous_2006_Nature_440_790
Author(s) : Strous M , Pelletier E , Mangenot S , Rattei T , Lehner A , Taylor MW , Horn M , Daims H , Bartol-Mavel D , Wincker P , Barbe V , Fonknechten N , Vallenet D , Segurens B , Schenowitz-Truong C , Medigue C , Collingro A , Snel B , Dutilh BE , Op den Camp HJ , van der Drift C , Cirpus I , van de Pas-Schoonen KT , Harhangi HR , van Niftrik L , Schmid M , Keltjens J , van de Vossenberg J , Kartal B , Meier H , Frishman D , Huynen MA , Mewes HW , Weissenbach J , Jetten MS , Wagner M , Le Paslier D
Ref : Nature , 440 :790 , 2006
Abstract : Anaerobic ammonium oxidation (anammox) has become a main focus in oceanography and wastewater treatment. It is also the nitrogen cycle's major remaining biochemical enigma. Among its features, the occurrence of hydrazine as a free intermediate of catabolism, the biosynthesis of ladderane lipids and the role of cytoplasm differentiation are unique in biology. Here we use environmental genomics--the reconstruction of genomic data directly from the environment--to assemble the genome of the uncultured anammox bacterium Kuenenia stuttgartiensis from a complex bioreactor community. The genome data illuminate the evolutionary history of the Planctomycetes and allow us to expose the genetic blueprint of the organism's special properties. Most significantly, we identified candidate genes responsible for ladderane biosynthesis and biological hydrazine metabolism, and discovered unexpected metabolic versatility.
ESTHER : Strous_2006_Nature_440_790
PubMedSearch : Strous_2006_Nature_440_790
PubMedID: 16598256
Gene_locus related to this paper: 9bact-q1py93 , 9bact-q1q3k9 , 9bact-q1q414

Title : The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution - van de Guchte_2006_Proc.Natl.Acad.Sci.U.S.A_103_9274
Author(s) : van de Guchte M , Penaud S , Grimaldi C , Barbe V , Bryson K , Nicolas P , Robert C , Oztas S , Mangenot S , Couloux A , Loux V , Dervyn R , Bossy R , Bolotin A , Batto JM , Walunas T , Gibrat JF , Bessieres P , Weissenbach J , Ehrlich SD , Maguin E
Ref : Proc Natl Acad Sci U S A , 103 :9274 , 2006
Abstract : Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) is a representative of the group of lactic acid-producing bacteria, mainly known for its worldwide application in yogurt production. The genome sequence of this bacterium has been determined and shows the signs of ongoing specialization, with a substantial number of pseudogenes and incomplete metabolic pathways and relatively few regulatory functions. Several unique features of the L. bulgaricus genome support the hypothesis that the genome is in a phase of rapid evolution. (i) Exceptionally high numbers of rRNA and tRNA genes with regard to genome size may indicate that the L. bulgaricus genome has known a recent phase of important size reduction, in agreement with the observed high frequency of gene inactivation and elimination; (ii) a much higher GC content at codon position 3 than expected on the basis of the overall GC content suggests that the composition of the genome is evolving toward a higher GC content; and (iii) the presence of a 47.5-kbp inverted repeat in the replication termination region, an extremely rare feature in bacterial genomes, may be interpreted as a transient stage in genome evolution. The results indicate the adaptation of L. bulgaricus from a plant-associated habitat to the stable protein and lactose-rich milk environment through the loss of superfluous functions and protocooperation with Streptococcus thermophilus.
ESTHER : van de Guchte_2006_Proc.Natl.Acad.Sci.U.S.A_103_9274
PubMedSearch : van de Guchte_2006_Proc.Natl.Acad.Sci.U.S.A_103_9274
PubMedID: 16754859
Gene_locus related to this paper: lacda-q1g8l1 , lacdb-q04ci8 , lacdb-q04cw3 , lacdl-pepx , lacdl-pip

Title : Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila - Vodovar_2006_Nat.Biotechnol_24_673
Author(s) : Vodovar N , Vallenet D , Cruveiller S , Rouy Z , Barbe V , Acosta C , Cattolico L , Jubin C , Lajus A , Segurens B , Vacherie B , Wincker P , Weissenbach J , Lemaitre B , Medigue C , Boccard F
Ref : Nat Biotechnol , 24 :673 , 2006
Abstract : Pseudomonas entomophila is an entomopathogenic bacterium that, upon ingestion, kills Drosophila melanogaster as well as insects from different orders. The complete sequence of the 5.9-Mb genome was determined and compared to the sequenced genomes of four Pseudomonas species. P. entomophila possesses most of the catabolic genes of the closely related strain P. putida KT2440, revealing its metabolically versatile properties and its soil lifestyle. Several features that probably contribute to its entomopathogenic properties were disclosed. Unexpectedly for an animal pathogen, P. entomophila is devoid of a type III secretion system and associated toxins but rather relies on a number of potential virulence factors such as insecticidal toxins, proteases, putative hemolysins, hydrogen cyanide and novel secondary metabolites to infect and kill insects. Genome-wide random mutagenesis revealed the major role of the two-component system GacS/GacA that regulates most of the potential virulence factors identified.
ESTHER : Vodovar_2006_Nat.Biotechnol_24_673
PubMedSearch : Vodovar_2006_Nat.Biotechnol_24_673
PubMedID: 16699499
Gene_locus related to this paper: pseae-PA1622 , psee4-q1i2k0 , psee4-q1i3a7 , psee4-q1i3n8 , psee4-q1i4y6 , psee4-q1i4z6 , psee4-q1i5s3 , psee4-q1i5s9 , psee4-q1i6r6 , psee4-q1i9a0 , psee4-q1i9c3 , psee4-q1i9h5 , psee4-q1i9t2 , psee4-q1iaa7 , psee4-q1ibu9 , psee4-q1icm5 , psee4-q1idp8 , psee4-q1idv7 , psee4-q1ie27 , psee4-q1ie44 , psee4-q1ifj6 , psee4-q1ifn8 , psee4-q1ig13 , psee4-q1ige4 , psep1-a5wax1 , psepg-b0kir7 , psepk-q88nk6 , psepu-METX , psepu-PHAZ , psepu-PIP , psepu-PP1617 , psepu-PP4178 , psepu-PP4551 , psee4-q1icg3

Title : Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125 - Medigue_2005_Genome.Res_15_1325
Author(s) : Medigue C , Krin E , Pascal G , Barbe V , Bernsel A , Bertin PN , Cheung F , Cruveiller S , D'Amico S , Duilio A , Fang G , Feller G , Ho C , Mangenot S , Marino G , Nilsson J , Parrilli E , Rocha EP , Rouy Z , Sekowska A , Tutino ML , Vallenet D , von Heijne G , Danchin A
Ref : Genome Res , 15 :1325 , 2005
Abstract : A considerable fraction of life develops in the sea at temperatures lower than 15 degrees C. Little is known about the adaptive features selected under those conditions. We present the analysis of the genome sequence of the fast growing Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. We find that it copes with the increased solubility of oxygen at low temperature by multiplying dioxygen scavenging while deleting whole pathways producing reactive oxygen species. Dioxygen-consuming lipid desaturases achieve both protection against oxygen and synthesis of lipids making the membrane fluid. A remarkable strategy for avoidance of reactive oxygen species generation is developed by P. haloplanktis, with elimination of the ubiquitous molybdopterin-dependent metabolism. The P. haloplanktis proteome reveals a concerted amino acid usage bias specific to psychrophiles, consistently appearing apt to accommodate asparagine, a residue prone to make proteins age. Adding to its originality, P. haloplanktis further differs from its marine counterparts with recruitment of a plasmid origin of replication for its second chromosome.
ESTHER : Medigue_2005_Genome.Res_15_1325
PubMedSearch : Medigue_2005_Genome.Res_15_1325
PubMedID: 16169927
Gene_locus related to this paper: pseht-q3icg6 , pseht-q3icm3 , pseht-q3icu1 , pseht-q3id97 , pseht-q3ida0 , pseht-q3idf4 , pseht-q3ie89 , pseht-q3ieu0 , pseht-q3iev9 , pseht-q3if93 , pseht-q3ifd8 , pseht-q3ife2 , pseht-q3ig70 , pseht-q3igp2 , pseht-q3igv0 , pseht-q3ihr6 , pseht-q3ii38 , pseht-q3iid7 , pseht-q3iip2 , pseht-q3iir1 , pseht-q3iis4 , pseht-q3ijn3 , pseht-q3ijt3 , pseht-q3ijy1 , pseht-q3ijy8 , pseht-q3ik03 , pseht-q3ikv5 , pseht-q3il66 , pseht-q3ik88

Title : Genome evolution in yeasts - Dujon_2004_Nature_430_35
Author(s) : Dujon B , Sherman D , Fischer G , Durrens P , Casaregola S , Lafontaine I , De Montigny J , Marck C , Neuveglise C , Talla E , Goffard N , Frangeul L , Aigle M , Anthouard V , Babour A , Barbe V , Barnay S , Blanchin S , Beckerich JM , Beyne E , Bleykasten C , Boisrame A , Boyer J , Cattolico L , Confanioleri F , de Daruvar A , Despons L , Fabre E , Fairhead C , Ferry-Dumazet H , Groppi A , Hantraye F , Hennequin C , Jauniaux N , Joyet P , Kachouri R , Kerrest A , Koszul R , Lemaire M , Lesur I , Ma L , Muller H , Nicaud JM , Nikolski M , Oztas S , Ozier-Kalogeropoulos O , Pellenz S , Potier S , Richard GF , Straub ML , Suleau A , Swennen D , Tekaia F , Wesolowski-Louvel M , Westhof E , Wirth B , Zeniou-Meyer M , Zivanovic I , Bolotin-Fukuhara M , Thierry A , Bouchier C , Caudron B , Scarpelli C , Gaillardin C , Weissenbach J , Wincker P , Souciet JL
Ref : Nature , 430 :35 , 2004
Abstract : Identifying the mechanisms of eukaryotic genome evolution by comparative genomics is often complicated by the multiplicity of events that have taken place throughout the history of individual lineages, leaving only distorted and superimposed traces in the genome of each living organism. The hemiascomycete yeasts, with their compact genomes, similar lifestyle and distinct sexual and physiological properties, provide a unique opportunity to explore such mechanisms. We present here the complete, assembled genome sequences of four yeast species, selected to represent a broad evolutionary range within a single eukaryotic phylum, that after analysis proved to be molecularly as diverse as the entire phylum of chordates. A total of approximately 24,200 novel genes were identified, the translation products of which were classified together with Saccharomyces cerevisiae proteins into about 4,700 families, forming the basis for interspecific comparisons. Analysis of chromosome maps and genome redundancies reveal that the different yeast lineages have evolved through a marked interplay between several distinct molecular mechanisms, including tandem gene repeat formation, segmental duplication, a massive genome duplication and extensive gene loss.
ESTHER : Dujon_2004_Nature_430_35
PubMedSearch : Dujon_2004_Nature_430_35
PubMedID: 15229592
Gene_locus related to this paper: canga-apth1 , canga-ppme1 , canga-q6fik7 , canga-q6fiv5 , canga-q6fiw8 , canga-q6fj11 , canga-q6fjh6 , canga-q6fjl0 , canga-q6fjr8 , canga-q6fkj6 , canga-q6fkm9 , canga-q6fku7 , canga-q6fl14 , canga-q6flb5 , canga-q6fle9 , canga-q6flk8 , canga-q6fly1 , canga-q6fly9 , canga-q6fmz4 , canga-q6fnx4 , canga-q6fp28 , canga-q6fpa8 , canga-q6fpi6 , canga-q6fpv7 , canga-q6fpw6 , canga-q6fqj3 , canga-q6fr97 , canga-q6frt7 , canga-q6ftm9 , canga-q6ftu0 , canga-q6ftv9 , canga-q6ftz9 , canga-q6fuf8 , canga-q6fv41 , canga-q6fvu3 , canga-q6fw36 , canga-q6fw94 , canga-q6fwk6 , canga-q6fwm0 , canga-q6fxc7 , canga-q6fxd7 , debha-apth1 , debha-atg15 , debha-b5rtk1 , debha-b5rub4 , debha-b5rue8 , debha-b5rue9 , debha-bna7 , debha-ppme1 , debha-q6bgx3 , debha-q6bh69 , debha-q6bhb8 , debha-q6bhc1 , debha-q6bhd0 , debha-q6bhj7 , debha-q6bi97 , debha-q6biq7 , debha-q6bj53 , debha-q6bkd8 , debha-q6bks1 , debha-q6bky4 , debha-q6bm63 , debha-q6bmh3 , debha-q6bn89 , debha-q6bnj6 , debha-q6bp08 , debha-q6bpb4 , debha-q6bpc0 , debha-q6bpc6 , debha-q6bq10 , debha-q6bq11 , debha-q6bqd9 , debha-q6bqj6 , debha-q6br33 , debha-q6br93 , debha-q6brg1 , debha-q6brw7 , debha-q6bs23 , debha-q6bsc3 , debha-q6bsl8 , debha-q6bsx6 , debha-q6bta5 , debha-q6bty5 , debha-q6btz0 , debha-q6bu73 , debha-q6buk9 , debha-q6but7 , debha-q6bvc4 , debha-q6bvg4 , debha-q6bvg8 , debha-q6bvp4 , debha-q6bw82 , debha-q6bxr7 , debha-q6bxu9 , debha-q6bym5 , debha-q6byn7 , debha-q6bzj8 , debha-q6bzk2 , debha-q6bzm5 , klula-apth1 , klula-ppme1 , klula-q6cin9 , klula-q6ciu6 , klula-q6cj47 , klula-q6cjc8 , klula-q6cjq9 , klula-q6cjs1 , klula-q6cjv9 , klula-q6ckd7 , klula-q6ckk4 , klula-q6ckx4 , klula-q6cl20 , klula-q6clm1 , klula-q6cly8 , klula-q6clz7 , klula-q6cm48 , klula-q6cm49 , klula-q6cmt5 , klula-q6cn71 , klula-q6cnm1 , klula-q6cr74 , klula-q6cr90 , klula-q6crs0 , klula-q6crv8 , klula-q6crz9 , klula-q6cst8 , klula-q6csv8 , klula-q6ctp8 , klula-q6cu02 , klula-q6cu78 , klula-q6cu79 , klula-q6cuv3 , klula-q6cvd3 , klula-q6cw70 , klula-q6cw92 , klula-q6cwu7 , klula-q6cx84 , klula-q6cxa3 , klula-q6cy41 , yarli-apth1 , yarli-atg15 , yarli-BST1B , yarli-lip2 , yarli-LIP3 , yarli-LIP4 , yarli-LIP5 , yarli-LIP7 , yarli-LIP8 , yarli-lipa1 , yarli-ppme1 , yarli-q6bzp1 , yarli-q6bzv7 , yarli-q6c1f5 , yarli-q6c1f7 , yarli-q6c1r3 , yarli-q6c2z2 , yarli-q6c3h1 , yarli-q6c3i6 , yarli-q6c3l1 , yarli-q6c3u6 , yarli-q6c4h8 , yarli-q6c5j1 , yarli-q6c5m4 , yarli-q6c6m4 , yarli-q6c6p7 , yarli-q6c6v2 , yarli-q6c7h3 , yarli-q6c7i7 , yarli-q6c7j5 , yarli-q6c7y6 , yarli-q6c8m4 , yarli-q6c8q4 , yarli-q6c8u4 , yarli-q6c8y2 , yarli-q6c9r0 , yarli-q6c9r1 , yarli-q6c9u0 , yarli-q6c9v4 , yarli-q6c209 , yarli-q6c225 , yarli-q6c493 , yarli-q6c598 , yarli-q6c687 , yarli-q6c822 , yarli-q6cau6 , yarli-q6cax2 , yarli-q6caz1 , yarli-q6cb63 , yarli-q6cba7 , yarli-q6cbb1 , yarli-q6cbe6 , yarli-q6cby1 , yarli-q6ccr0 , yarli-q6cdg1 , yarli-q6cdi6 , yarli-q6cdv9 , yarli-q6ce37 , yarli-q6ceg0 , yarli-q6cep3 , yarli-q6cey5 , yarli-q6cf60 , yarli-q6cfp3 , yarli-q6cfx2 , yarli-q6cg13 , yarli-q6cg27 , yarli-q6cgj3 , yarli-q6chb8 , yarli-q6ci59 , yarli-q6c748 , canga-q6fpj0 , klula-q6cp11 , yarli-q6c4p0 , debha-q6btp5 , debha-kex1

Title : Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium - Barbe_2004_Nucleic.Acids.Res_32_5766
Author(s) : Barbe V , Vallenet D , Fonknechten N , Kreimeyer A , Oztas S , Labarre L , Cruveiller S , Robert C , Duprat S , Wincker P , Ornston LN , Weissenbach J , Marliere P , Cohen GN , Medigue C
Ref : Nucleic Acids Research , 32 :5766 , 2004
Abstract : Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas putida. Unlike these bacteria, the Acinetobacter ADP1 is highly competent for natural transformation which affords extraordinary convenience for genetic manipulation. The circular chromosome of the Acinetobacter ADP1, presented here, encodes 3325 predicted coding sequences, of which 60% have been classified based on sequence similarity to other documented proteins. The close evolutionary proximity of Acinetobacter and Pseudomonas species, as judged by the sequences of their 16S RNA genes and by the highest level of bidirectional best hits, contrasts with the extensive divergence in the GC content of their DNA (40 versus 62%). The chromosomes also differ significantly in size, with the Acinetobacter ADP1 chromosome <60% of the length of the Pseudomonas counterparts. Genome analysis of the Acinetobacter ADP1 revealed genes for metabolic pathways involved in utilization of a large variety of compounds. Almost all of these genes, with orthologs that are scattered in other species, are located in five major 'islands of catabolic diversity', now an apparent 'archipelago of catabolic diversity', within one-quarter of the overall genome. Acinetobacter ADP1 displays many features of other aerobic soil bacteria with metabolism oriented toward the degradation of organic compounds found in their natural habitat. A distinguishing feature of this genome is the absence of a gene corresponding to pyruvate kinase, the enzyme that generally catalyzes the terminal step in conversion of carbohydrates to pyruvate for respiration by the citric acid cycle. This finding supports the view that the cycle itself is centrally geared to the catabolic capabilities of this exceptionally versatile organism.
ESTHER : Barbe_2004_Nucleic.Acids.Res_32_5766
PubMedSearch : Barbe_2004_Nucleic.Acids.Res_32_5766
PubMedID: 15514110
Gene_locus related to this paper: aciad-q6f6s6 , aciad-q6f6x6 , aciad-q6f6x7 , aciad-q6f6z2 , aciad-q6f7m0 , aciad-q6f7y3 , aciad-q6f8t1 , aciad-q6f8v6 , aciad-q6f8x2 , aciad-q6f9b1 , aciad-q6f9f4 , aciad-q6f933 , aciad-q6f951 , aciad-q6fa15 , aciad-q6fa93 , aciad-q6fag8 , aciad-q6fak6 , aciad-q6fal1 , aciad-q6fas4 , aciad-q6faz3 , aciad-q6fbp9 , aciad-q6fbr2 , aciad-q6fbr5 , aciad-q6fc40 , aciad-q6fd43 , aciad-q6fd56 , aciad-q6fdd3 , aciad-q6fdh2 , aciad-q6fe39 , aciad-q6feb1 , aciad-q6fen4 , aciad-q6feq3 , aciad-q6ff86 , aciad-q6ffz9 , aciad-q8rlz8 , acica-elh1 , acica-elh2 , acica-estB , acica-este2 , acica-este3

Title : Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome - Dufresne_2003_Proc.Natl.Acad.Sci.U.S.A_100_10020
Author(s) : Dufresne A , Salanoubat M , Partensky F , Artiguenave F , Axmann IM , Barbe V , Duprat S , Galperin MY , Koonin EV , Le Gall F , Makarova KS , Ostrowski M , Oztas S , Robert C , Rogozin IB , Scanlan DJ , Tandeau de Marsac N , Weissenbach J , Wincker P , Wolf YI , Hess WR
Ref : Proc Natl Acad Sci U S A , 100 :10020 , 2003
Abstract : Prochlorococcus marinus, the dominant photosynthetic organism in the ocean, is found in two main ecological forms: high-light-adapted genotypes in the upper part of the water column and low-light-adapted genotypes at the bottom of the illuminated layer. P. marinus SS120, the complete genome sequence reported here, is an extremely low-light-adapted form. The genome of P. marinus SS120 is composed of a single circular chromosome of 1,751,080 bp with an average G+C content of 36.4%. It contains 1,884 predicted protein-coding genes with an average size of 825 bp, a single rRNA operon, and 40 tRNA genes. Together with the 1.66-Mbp genome of P. marinus MED4, the genome of P. marinus SS120 is one of the two smallest genomes of a photosynthetic organism known to date. It lacks many genes that are involved in photosynthesis, DNA repair, solute uptake, intermediary metabolism, motility, phototaxis, and other functions that are conserved among other cyanobacteria. Systems of signal transduction and environmental stress response show a particularly drastic reduction in the number of components, even taking into account the small size of the SS120 genome. In contrast, housekeeping genes, which encode enzymes of amino acid, nucleotide, cofactor, and cell wall biosynthesis, are all present. Because of its remarkable compactness, the genome of P. marinus SS120 might approximate the minimal gene complement of a photosynthetic organism.
ESTHER : Dufresne_2003_Proc.Natl.Acad.Sci.U.S.A_100_10020
PubMedSearch : Dufresne_2003_Proc.Natl.Acad.Sci.U.S.A_100_10020
PubMedID: 12917486
Gene_locus related to this paper: proma-MHPC2 , proma-PRO1109 , proma-q7v9n9 , proma-q7vb48 , proma-q7vb60 , proma-q7vbe1 , proma-q7vbl5 , proma-q7vcg4 , proma-q7vdr9 , proma-q7ved1

Title : An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi - Cohen_2003_Mol.Microbiol_47_1495
Author(s) : Cohen GN , Barbe V , Flament D , Galperin M , Heilig R , Lecompte O , Poch O , Prieur D , Querellou J , Ripp R , Thierry JC , Van der Oost J , Weissenbach J , Zivanovic Y , Forterre P
Ref : Molecular Microbiology , 47 :1495 , 2003
Abstract : The hyperthermophilic euryarchaeon Pyrococcus abyssi and the related species Pyrococcus furiosus and Pyrococcus horikoshii, whose genomes have been completely sequenced, are presently used as model organisms in different laboratories to study archaeal DNA replication and gene expression and to develop genetic tools for hyperthermophiles. We have performed an extensive re-annotation of the genome of P. abyssi to obtain an integrated view of its phylogeny, molecular biology and physiology. Many new functions are predicted for both informational and operational proteins. Moreover, several candidate genes have been identified that might encode missing links in key metabolic pathways, some of which have unique biochemical features. The great majority of Pyrococcus proteins are typical archaeal proteins and their phylogenetic pattern agrees with its position near the root of the archaeal tree. However, proteins probably from bacterial origin, including some from mesophilic bacteria, are also present in the P. abyssi genome.
ESTHER : Cohen_2003_Mol.Microbiol_47_1495
PubMedSearch : Cohen_2003_Mol.Microbiol_47_1495
PubMedID: 12622808
Gene_locus related to this paper: pyrab-PAB0762 , pyrab-PAB1050 , pyrab-PAB1300 , pyrab-PAB2176 , pyrab-PYRAB14490

Title : Mechanisms of evolution in Rickettsia conorii and R. prowazekii - Ogata_2001_Science_293_2093
Author(s) : Ogata H , Audic S , Renesto-Audiffren P , Fournier PE , Barbe V , Samson D , Roux V , Cossart P , Weissenbach J , Claverie JM , Raoult D
Ref : Science , 293 :2093 , 2001
Abstract : Rickettsia conorii is an obligate intracellular bacterium that causes Mediterranean spotted fever in humans. We determined the 1,268,755-nucleotide complete genome sequence of R. conorii, containing 1374 open reading frames. This genome exhibits 804 of the 834 genes of the previously determined R. prowazekii genome plus 552 supplementary open reading frames and a 10-fold increase in the number of repetitive elements. Despite these differences, the two genomes exhibit a nearly perfect colinearity that allowed the clear identification of different stages of gene alterations with gene remnants and 37 genes split in 105 fragments, of which 59 are transcribed. A 38-kilobase sequence inversion was dated shortly after the divergence of the genus.
ESTHER : Ogata_2001_Science_293_2093
PubMedSearch : Ogata_2001_Science_293_2093
PubMedID: 11557893
Gene_locus related to this paper: ricco-PTRB , ricco-RC0464 , ricco-RC0603 , ricco-RC0617 , ricco-RC0713 , ricco-RC1147 , ricco-RC1296 , ricpr-RP681 , ricco-RC1136

Title : Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi - Katinka_2001_Nature_414_450
Author(s) : Katinka MD , Duprat S , Cornillot E , Metenier G , Thomarat F , Prensier G , Barbe V , Peyretaillade E , Brottier P , Wincker P , Delbac F , El Alaoui H , Peyret P , Saurin W , Gouy M , Weissenbach J , Vivares CP
Ref : Nature , 414 :450 , 2001
Abstract : Microsporidia are obligate intracellular parasites infesting many animal groups. Lacking mitochondria and peroxysomes, these unicellular eukaryotes were first considered a deeply branching protist lineage that diverged before the endosymbiotic event that led to mitochondria. The discovery of a gene for a mitochondrial-type chaperone combined with molecular phylogenetic data later implied that microsporidia are atypical fungi that lost mitochondria during evolution. Here we report the DNA sequences of the 11 chromosomes of the approximately 2.9-megabase (Mb) genome of Encephalitozoon cuniculi (1,997 potential protein-coding genes). Genome compaction is reflected by reduced intergenic spacers and by the shortness of most putative proteins relative to their eukaryote orthologues. The strong host dependence is illustrated by the lack of genes for some biosynthetic pathways and for the tricarboxylic acid cycle. Phylogenetic analysis lends substantial credit to the fungal affiliation of microsporidia. Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle.
ESTHER : Katinka_2001_Nature_414_450
PubMedSearch : Katinka_2001_Nature_414_450
PubMedID: 11719806
Gene_locus related to this paper: enccu-ECU07.0900 , enccu-ECU09.0730 , enccu-q8sut7 , enccu-Q8SVX3

Title : The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis - Chambaud_2001_Nucleic.Acids.Res_29_2145
Author(s) : Chambaud I , Heilig R , Ferris S , Barbe V , Samson D , Galisson F , Moszer I , Dybvig K , Wroblewski H , Viari A , Rocha EP , Blanchard A
Ref : Nucleic Acids Research , 29 :2145 , 2001
Abstract : Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.
ESTHER : Chambaud_2001_Nucleic.Acids.Res_29_2145
PubMedSearch : Chambaud_2001_Nucleic.Acids.Res_29_2145
PubMedID: 11353084
Gene_locus related to this paper: mycpu-MYPU.0330 , mycpu-MYPU.0350 , mycpu-MYPU.0360 , mycpu-MYPU.0370 , mycpu-MYPU.0380 , mycpu-MYPU.6950