| Title : Molecular forms of acetyl- and butyrylcholinesterase in human glioma - Saez-Valero_1996_Neurosci.Lett_206_173 |
| Author(s) : Saez-Valero J , Poza-Cisneros G , Vidal CJ |
| Ref : Neuroscience Letters , 206 :173 , 1996 |
| Abstract : Specimens of astrocytoma, oligodendroglioma and medulloblastoma were sequentially extracted with saline and saline-Triton X-100 buffers. Acetyl- (AChE) and butyrylcholinesterase (BCHE) activities were assayed in the soluble fractions, these being further analyzed to establish the distribution of molecular forms. All the tumors tested showed AChE and BCHE activities, the measured AChE/BCHE ratios being unrelated to the malignant grading. Hydrophilic and amphiphilic AChE and BCHE tetramers, amphiphilic AChE dimers and monomers, and hydrophilic BCHE monomers were identified in all the tumors analyzed. The amphiphilic behavior of the enzyme forms was assessed by sedimentation analysis and hydrophobic chromatography on phenyl-Agarose. A small fraction of glioma AChE monomers was released as, or transformed into, hydrophilic forms by incubation with phosphatidylinositol-specific phospholipase C (PIPLC). These data suggest that AChE monomers bearing distinct hydrophobic domains coexist in human glioma. |
| ESTHER : Saez-Valero_1996_Neurosci.Lett_206_173 |
| PubMedSearch : Saez-Valero_1996_Neurosci.Lett_206_173 |
| PubMedID: 8710179 |
Saez-Valero J, Poza-Cisneros G, Vidal CJ (1996)
Molecular forms of acetyl- and butyrylcholinesterase in human glioma
Neuroscience Letters
206 :173
Saez-Valero J, Poza-Cisneros G, Vidal CJ (1996)
Neuroscience Letters
206 :173