Title : Influence of organic co-solvents on the activity and substrate specificity of feruloyl esterases - Faulds_2011_Bioresour.Technol_102_4962 |
Author(s) : Faulds CB , Perez-Boada M , Martinez AT |
Ref : Bioresour Technol , 102 :4962 , 2011 |
Abstract :
Organic co-solvents can expand the use of enzymes in lignocellulose deconstruction through making substrates more soluble and thus more accessible. In choosing the most adequate co-solvent for feruloyl esterases, hydrolysis of methyl p-hydroxycinnamates by three pure enzymes (and a multi-enzyme preparation) was evaluated. Low concentrations of dimethylsulfoxide (DMSO) enhanced hydrolysis by two of the enzymes while at levels >20%, activity was reduced. DMSO also enhanced acetyl esterase-type activity of the enzymes. The co-solvent effect was different for each enzyme-substrate couple, indicating that other factors are also involved. Kinetic studies with a Talaromyces stipitatus feruloyl esterase showed low concentrations of dimethylsulfoxide enhanced the hydrolytic rate while K(m) also increased. Moreover, long-term incubation (96 h) of an Aspergillus niger feruloyl esterase in dimethylsulfoxide:water provided to the enzyme the ability to hydrolyze methyl p-coumarate, suggesting an active-site re-arrangement. Dimethylsulfoxide (10-30%) is proposed as an adequate co-solvent for feruloyl esterase treatment of water-insoluble substrates. |
PubMedSearch : Faulds_2011_Bioresour.Technol_102_4962 |
PubMedID: 21354789 |
Faulds CB, Perez-Boada M, Martinez AT (2011)
Influence of organic co-solvents on the activity and substrate specificity of feruloyl esterases
Bioresour Technol
102 :4962
Faulds CB, Perez-Boada M, Martinez AT (2011)
Bioresour Technol
102 :4962