Title : Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application - de Saint Germain_2022_Front.Plant.Sci_13_887347 |
Author(s) : de Saint Germain A , Clave G , Schouveiler P , Pillot JP , Singh AV , Chevalier A , Daignan Fornier S , Guillory A , Bonhomme S , Rameau C , Boyer FD |
Ref : Front Plant Sci , 13 :887347 , 2022 |
Abstract :
Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clave (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants. |
PubMedSearch : de Saint Germain_2022_Front.Plant.Sci_13_887347 |
PubMedID: 35720613 |
Inhibitor | Scopoletin |
Substrate | Xilatone-Red Yoshimulactone-Green 3'-Methyl-GR24 desmethyl-GR24 GC242 GC240 GC486 DiFMU-acetate |
de Saint Germain A, Clave G, Schouveiler P, Pillot JP, Singh AV, Chevalier A, Daignan Fornier S, Guillory A, Bonhomme S, Rameau C, Boyer FD (2022)
Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application
Front Plant Sci
13 :887347
de Saint Germain A, Clave G, Schouveiler P, Pillot JP, Singh AV, Chevalier A, Daignan Fornier S, Guillory A, Bonhomme S, Rameau C, Boyer FD (2022)
Front Plant Sci
13 :887347