Clave G

References (7)

Title : Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application - de Saint Germain_2022_Front.Plant.Sci_13_887347
Author(s) : de Saint Germain A , Clave G , Schouveiler P , Pillot JP , Singh AV , Chevalier A , Daignan Fornier S , Guillory A , Bonhomme S , Rameau C , Boyer FD
Ref : Front Plant Sci , 13 :887347 , 2022
Abstract : Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clave (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.
ESTHER : de Saint Germain_2022_Front.Plant.Sci_13_887347
PubMedSearch : de Saint Germain_2022_Front.Plant.Sci_13_887347
PubMedID: 35720613

Title : A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically - de Saint Germain_2021_Plant.Commun_2_100166
Author(s) : de Saint Germain A , Jacobs A , Brun G , Pouvreau JB , Braem L , Cornu D , Clave G , Baudu E , Steinmetz V , Servajean V , Wicke S , Gevaert K , Simier P , Goormachtig S , Delavault P , Boyer FD
Ref : Plant Commun , 2 :100166 , 2021
Abstract : Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe. In order to germinate, it must perceive various structurally divergent host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identify five putative SL receptors in P. ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination. We demonstrate the high plasticity of PrKAI2d3, which allows it to interact with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with bioactivity comparable to that of ITCs. This study demonstrates that P. ramosa has extended its signal perception system during evolution, a fact that should be considered for the development of specific and efficient biocontrol methods.
ESTHER : de Saint Germain_2021_Plant.Commun_2_100166
PubMedSearch : de Saint Germain_2021_Plant.Commun_2_100166
PubMedID: 34746757
Gene_locus related to this paper: phera-PrKAI2c , phera-PrKAI2d1 , phera-PrKAI2d2 , phera-PrKAI2d3 , phera-PrKAI2d4

Title : Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies - de Saint Germain_2021_Methods.Mol.Biol_2309_219
Author(s) : de Saint Germain A , Clave G , Boyer FD
Ref : Methods Mol Biol , 2309 :219 , 2021
Abstract : In this chapter, we will describe a method we set up to synthesize two profluorescent strigolactone (SL) mimic probes (GC240 and GC242) and the optimized protocols developed to study the enzymatic properties of various strigolactone receptors. The Arabidopsis AtD14 SL receptor is used here as a model for this purpose.
ESTHER : de Saint Germain_2021_Methods.Mol.Biol_2309_219
PubMedSearch : de Saint Germain_2021_Methods.Mol.Biol_2309_219
PubMedID: 34028690

Title : A Phelipanche ramosa KAI2 Protein Perceives enzymatically Strigolactones and Isothiocyanates - de Saint Germain_2020_Biorxiv__
Author(s) : de Saint Germain A , Jacobs A , Brun G , Pouvreau JB , Braem L , Cornu D , Clave G , Baudu E , Steinmetz V , Servajean V , Wicke S , Gevaert K , Simier P , Goormachti S , Delavault P , Boyer FD
Ref : Biorxiv , : , 2020
Abstract : Phelipanche ramosa is an obligate root-parasitic weed threatening major crops in central Europe. For its germination, it has to perceive various structurally diverging host-exuded signals, including isothiocyanates (ITCs) and strigolactones (SLs). However, the receptors involved are still uncharacterized. Here, we identified five putative SL receptors in P. ramosa, of which PrKAI2d3 is involved in seed germination stimulation. We established the high plasticity of PrKAI2d3, allowing interaction with different chemicals, including ITCs. The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants. We provide evidence that the PrKAI2d3 enzymatic activity confers hypersensitivity to SLs. Additionally, we demonstrated that methylbutenolide-OH binds PrKAI2d3 and stimulates P. ramosa germination with a bioactivity comparable to that of ITCs. This study highlights that P. ramosa has extended its signal perception system during evolution, a fact to be considered in the development of specific and efficient biocontrol methods.
ESTHER : de Saint Germain_2020_Biorxiv__
PubMedSearch : de Saint Germain_2020_Biorxiv__
PubMedID:

Title : Validated Method for Strigolactone Quantification by Ultra High-Performance Liquid Chromatography - Electrospray Ionisation Tandem Mass Spectrometry Using Novel Deuterium Labelled Standards - Boutet-Mercey_2018_Phytochem.Anal_29_59
Author(s) : Boutet-Mercey S , Perreau F , Roux A , Clave G , Pillot JP , Schmitz-Afonso I , Touboul D , Mouille G , Rameau C , Boyer FD
Ref : Phytochem Anal , 29 :59 , 2018
Abstract : INTRODUCTION: Strigolactones (SLs) are important plant hormones. They are difficult to analyse because they occur in very small concentrations especially in comparison with other plant hormones and other substances can interfere with their detection. OBJECTIVE: To develop a procedure for the extraction, purification and quantification of SLs from plant roots. METHODOLOGY: Samples were prepared by extraction of plant root tissues with ethyl acetate. Then the extracts were further purified with silica column chromatography. The natural SLs in the final extracts were quantified using novel deuterium labelled SLs. The results of the methodology were compared with those of the procedure of Yoneyama and coworkers. RESULTS: This procedure required about 1-g root samples to detect and quantify simultaneously the SLs (orobanchyl acetate and fabacyl acetate) concentration with high reliability. CONCLUSION: A method was developed for determining endogenous fabacyl acetate and orobanchyl acetate in plant tissue based on novel deuterium labelled standards. A method of orobanchol quantification using a synthetic SL GR24 as internal standard was proposed. Copyright 2017 John Wiley & Sons, Ltd.
ESTHER : Boutet-Mercey_2018_Phytochem.Anal_29_59
PubMedSearch : Boutet-Mercey_2018_Phytochem.Anal_29_59
PubMedID: 28851101

Title : An histidine covalent receptor and butenolide complex mediates strigolactone perception - de Saint Germain_2016_Nat.Chem.Biol_12_787
Author(s) : de Saint Germain A , Clave G , Badet-Denisot MA , Pillot JP , Cornu D , Le Caer JP , Burger M , Pelissier F , Retailleau P , Turnbull C , Bonhomme S , Chory J , Rameau C , Boyer FD
Ref : Nat Chemical Biology , 12 :787 , 2016
Abstract : Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of alpha/beta-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.
ESTHER : de Saint Germain_2016_Nat.Chem.Biol_12_787
PubMedSearch : de Saint Germain_2016_Nat.Chem.Biol_12_787
PubMedID: 27479744
Gene_locus related to this paper: pea-RMS3

Title : New strigolactone analogs as plant hormones with low activities in the rhizosphere - Boyer_2014_Mol.Plant_7_675
Author(s) : Boyer FD , de Saint Germain A , Pouvreau JB , Clave G , Pillot JP , Roux A , Rasmussen A , Depuydt S , Lauressergues D , Frei dit Frey N , Heugebaert TS , Stevens CV , Geelen D , Goormachtig S , Rameau C
Ref : Mol Plant , 7 :675 , 2014
Abstract : Strigolactones (SLs) are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules (23 (3'-methyl-GR24), 31 (thia-3'-methyl-debranone-like molecule)) already described, and a new one (AR36), for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the D-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules: 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. Detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants (pea rms1 and rms4, Arabidopsis max2 and, max4) for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination.
ESTHER : Boyer_2014_Mol.Plant_7_675
PubMedSearch : Boyer_2014_Mol.Plant_7_675
PubMedID: 24249726