Amplex-red
Amplex Red is a fluorescent probe that is widely used to detect hydrogen peroxide (H2O2) in a reaction where it is oxidised to resorufin by horseradish peroxidase (HRP) as a catalyst. Amplex Red is converted to resorufin by carboxylesterases 1 and 2 without requiring H2O2, horseradish peroxidase or oxygen. Caution is necessary in experimental procedures for the detection of H2O2 release from isolated mitochondria in tissue containing carboxylesterase. It probably is less important for large scale screening of cholinesterases inhibitors
General
Type : Phenoxazin, Oxazine, Derivative of Resorufin, Not AlphaBeta Hydrolase target, Fluorescent Probe
Chemical_Nomenclature : 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
Canonical SMILES : CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O
InChI : InChI=1S\/C14H11NO4\/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15\/h2-7,17-18H,1H3
InChIKey : PKYCWFICOKSIHZ-UHFFFAOYSA-N
Other name(s) : 10-Acetyl-3,7-dihydroxyphenoxazine || 1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone || ADHP || Amplex red
MW : 257.24
Formula : C14H11NO4
CAS_number : 119171-73-2
PubChem : 167453
UniChem : PKYCWFICOKSIHZ-UHFFFAOYSA-N
Target
Structures : No structure
Families : Carb_B_Chordata
References (4)
| Title : Discovery of dual cation-pi inhibitors of acetylcholinesterase: design, synthesis and biological evaluation - Damuka_2020_Pharmacol.Rep__ |
| Author(s) : Damuka N , Kammari K , Potshangbam AM , Rathore RS , Kondapi AK , Vindal V |
| Ref : Pharmacol Rep , : , 2020 |
| Abstract : |
| PubMedSearch : Damuka_2020_Pharmacol.Rep__ |
| PubMedID: 32200493 |
| Title : Identification of acetylcholinesterase inhibitors using homogenous cell-based assays in quantitative high-throughput screening platforms - Li_2017_Biotechnol.J_12_ |
| Author(s) : Li S , Huang R , Solomon S , Liu Y , Zhao B , Santillo MF , Xia M |
| Ref : Biotechnol J , 12 : , 2017 |
| Abstract : |
| PubMedSearch : Li_2017_Biotechnol.J_12_ |
| PubMedID: 28294544 |
| Title : Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays - Miwa_2016_Free.Radic.Biol.Med_90_173 |
| Author(s) : Miwa S , Treumann A , Bell A , Vistoli G , Nelson G , Hay S , von Zglinicki T |
| Ref : Free Radic Biol Med , 90 :173 , 2016 |
| Abstract : |
| PubMedSearch : Miwa_2016_Free.Radic.Biol.Med_90_173 |
| PubMedID: 26577176 |
| Title : A fluorescence assay for measuring acetylcholinesterase activity in rat blood and a human neuroblastoma cell line (SH-SY5Y) - Santillo_2015_J.Pharmacol.Toxicol.Methods_76_15 |
| Author(s) : Santillo MF , Liu Y |
| Ref : Journal of Pharmacological & Toxicological Methods , 76 :15 , 2015 |
| Abstract : |
| PubMedSearch : Santillo_2015_J.Pharmacol.Toxicol.Methods_76_15 |
| PubMedID: 26165232 |