Coumarinylphenylmaleimide
This chemiluminescent probe is not substrate but react with a product of reaction of a substrate hydrolyzed by an enzyme: this compound reacts with thiocholine after hydrolysis of acetylthiocholine by cholinesterases. A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. Will react with any hyperreactive sulfhydryls. also used to quantitate sulfhydrils. Excitation wavelength is 390 nm, and the emission intensity is monitored at 473 nm.
General
Type : Chemiluminescent Probe, Thiocholine reactant, Fluorescent Probe, Coumarin
Chemical_Nomenclature : 1-[4-[7-(diethylamino)-4-methyl-2-oxochromen-3-yl]phenyl]pyrrole-2,5-dione
Canonical SMILES : CCN(CC)C1=CC2=C(C=C1)C(=C(C(=O)O2)C3=CC=C(C=C3)N4C(=O)C=CC4=O)C
InChI : InChI=1S\/C24H22N2O4\/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28\/h6-14H,4-5H2,1-3H3
InChIKey : YGIABALXNBVHBX-UHFFFAOYSA-N
Other name(s) : CPM || Demcpm || 3-Mmdac || 3-(4-Maleimidophenyl)-4-methyl-7-(diethylamino)coumarin || N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide
MW : 402.4
Formula : C24H22N2O4
CAS_number : 76877-33-3
PubChem : 122042
UniChem : YGIABALXNBVHBX-UHFFFAOYSA-N
Target
Structures : No structure
Families : No family
References (3)
| Title : Fluorescent method for evaluation of cholinesterase inhibitors - Gainullina_2006_Bull.Exp.Biol.Med_142_751 |
| Author(s) : Gainullina ET , Kondratyev KV , Ryzhikov SB , Taranchenko VF |
| Ref : Bulletin of Experimental Biology & Medicine , 142 :751 , 2006 |
| Abstract : |
| PubMedSearch : Gainullina_2006_Bull.Exp.Biol.Med_142_751 |
| PubMedID: 17603688 |
| Title : Phytoestrogens genistein and daidzin enhance the acetylcholinesterase activity of the rat pheochromocytoma cell line PC12 by binding to the estrogen receptor - Isoda_2002_Cytotech_40_117 |
| Author(s) : Isoda H , Talorete TP , Kimura M , Maekawa T , Inamori Y , Nakajima N , Seki H |
| Ref : Cytotechnology , 40 :117 , 2002 |
| Abstract : |
| PubMedSearch : Isoda_2002_Cytotech_40_117 |
| PubMedID: 19003112 |
| Title : A microfluorometric assay for cholinesterases, suitable for multiple kinetic determinations of picomoles of released thiocholine - Parvari_1983_Anal.Biochem_133_450 |
| Author(s) : Parvari R , Pecht I , Soreq H |
| Ref : Analytical Biochemistry , 133 :450 , 1983 |
| Abstract : |
| PubMedSearch : Parvari_1983_Anal.Biochem_133_450 |
| PubMedID: 6638505 |