Drawid A

References (1)

Title : Subcellular localization of the yeast proteome - Kumar_2002_Genes.Dev_16_707
Author(s) : Kumar A , Agarwal S , Heyman JA , Matson S , Heidtman M , Piccirillo S , Umansky L , Drawid A , Jansen R , Liu Y , Cheung KH , Miller P , Gerstein M , Roeder GS , Snyder M
Ref : Genes Dev , 16 :707 , 2002
Abstract : Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http:\/\/ygac.med.yale.edu.
ESTHER : Kumar_2002_Genes.Dev_16_707
PubMedSearch : Kumar_2002_Genes.Dev_16_707
PubMedID: 11914276
Gene_locus related to this paper: yeast-mgll