Kumar_2002_Genes.Dev_16_707

Reference

Title : Subcellular localization of the yeast proteome - Kumar_2002_Genes.Dev_16_707
Author(s) : Kumar A , Agarwal S , Heyman JA , Matson S , Heidtman M , Piccirillo S , Umansky L , Drawid A , Jansen R , Liu Y , Cheung KH , Miller P , Gerstein M , Roeder GS , Snyder M
Ref : Genes Dev , 16 :707 , 2002
Abstract :

Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http:\/\/ygac.med.yale.edu.

PubMedSearch : Kumar_2002_Genes.Dev_16_707
PubMedID: 11914276
Gene_locus related to this paper: yeast-mgll

Related information

Gene_locus yeast-mgll

Citations formats

Kumar A, Agarwal S, Heyman JA, Matson S, Heidtman M, Piccirillo S, Umansky L, Drawid A, Jansen R, Liu Y, Cheung KH, Miller P, Gerstein M, Roeder GS, Snyder M (2002)
Subcellular localization of the yeast proteome
Genes Dev 16 :707

Kumar A, Agarwal S, Heyman JA, Matson S, Heidtman M, Piccirillo S, Umansky L, Drawid A, Jansen R, Liu Y, Cheung KH, Miller P, Gerstein M, Roeder GS, Snyder M (2002)
Genes Dev 16 :707