Agarwal S

References (13)

Title : Neuroprotective potential of hydroethanolic hull extract of Juglans regia L. on isoprenaline induced oxidative damage in brain of Wistar rats - Sharma_2021_Toxicol.Rep_8_223
Author(s) : Sharma P , Verma PK , Sood S , Pankaj NK , Agarwal S , Raina R
Ref : Toxicol Rep , 8 :223 , 2021
Abstract : The study was aimed at assessing isoprenaline (ISO) induced oxidative damage in brain of Wistar rats and its protection by hydroethanolic hull extract of Juglans regia. Administration of ISO significantly increases catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), malondialdehyde (MDA) and advanced oxidation protein product (AOPP) levels and significantly reduced activities of antioxidant status (TAS), total thiols (TTH), acetylcholinesterase (AChE), arylesterase (AE), and glutathione peroxidase (GPx) in rat brain. Histopathologically, neuronal degeneration, spongiosis and gliosis were seen in cerebral cortex after ISO administration. Pretreatment with hull extract restored TAS, TTH, AChE, CAT and SOD values. Additionally, significant reductions were noted in levels of MDA, AOPP, and severity of histomorphological changes in cerebral cortex following hull extract treatment. Altered antioxidant biomarkers along with histopathological changes indicate oxidative injury in rat brain following ISO administration. Repeated administration of J. regia hull extract demonstrating presence of neuroprotective properties against ISO induced oxidative damage in rat brain.
ESTHER : Sharma_2021_Toxicol.Rep_8_223
PubMedSearch : Sharma_2021_Toxicol.Rep_8_223
PubMedID: 33520664

Title : Protective propensity of bacoside A and bromelain on renal cholinesterases, gamma-Aminobutyric acid and serotonin level of Mus musculus intoxicated with dichlorvos - Agarwal_2016_Chem.Biol.Interact_261_139
Author(s) : Agarwal S , Chaudhary B , Bist R
Ref : Chemico-Biological Interactions , 261 :139 , 2016
Abstract : Current study established a protective action of bacoside A and bromelain against the toxic effects of dichlorvos in kidneys of mice. Experimental design included five groups. The first group was control. Mice of groups II, III and IV were administered doses of dichlorvos, bromelain and bacoside A respectively. In group V, mice were treated with both the antioxidants (bacoside A and bromelain) and dichlorvos. After 21 days of exposure of different doses, levels of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), gamma-aminobutyric acid (GABA) and serotonin were measured in renal tissues. Dichlorvos significantly reduced the kidney AChE (p < 0.001), BChE (p < 0.01) and GABA level (p < 0.01) compared to control. A simultaneous significant elevation in the serotonin level (p < 0.01) was recorded after dichlorvos exposure. Concomitant exposure of bacoside A and bromelain followed by dichlorvos treatment in group V not only restored, but increased the renal cholinesterases and GABA level. Meanwhile, a significant decline in serotonin level (p < 0.001) was revealed, compared to dichlorvos exposed mice. Bacoside A and bromelain occupy a tremendous antioxidant action in the mice kidneys and a combination of the same ameliorates the renal toxicity induced by dichlorvos.
ESTHER : Agarwal_2016_Chem.Biol.Interact_261_139
PubMedSearch : Agarwal_2016_Chem.Biol.Interact_261_139
PubMedID: 27899289

Title : Neuroprotective Role of Novel Triazine Derivatives by Activating Wnt\/beta Catenin Signaling Pathway in Rodent Models of Alzheimer's Disease - Sinha_2015_Mol.Neurobiol_52_638
Author(s) : Sinha A , Tamboli RS , Seth B , Kanhed AM , Tiwari SK , Agarwal S , Nair S , Giridhar R , Chaturvedi RK , Yadav MR
Ref : Molecular Neurobiology , 52 :638 , 2015
Abstract : It has been reported in the literature that cholinesterase inhibitors provide protection in Alzheimer's disease (AD). Recent reports have implicated triazine derivatives as cholinesterase inhibitors. These findings led us to investigate anti-cholinestrase property of some novel triazine derivatives synthesized in this laboratory. In vitro cholinesterase inhibition assay was performed using Ellman method. The potent compounds screened out from in vitro assay were further evaluated using scopolamine-induced amnesic mice model. Further, in vitro reactive oxygen species (ROS) scavenging and anti-apoptotic property of the potent compounds were demonstrated against Abeta1-42-induced neurotoxicity in rat hippocampal cells. Their neuroprotective role was assessed using Abeta1-42-induced Alzheimer's-like phenotype in rats. Further, the role of compounds on the activation of the Wnt/beta-catenin pathway was studied. The results showed that the chosen compounds are having protective effect in Alzheimer's-like condition; the ex vivo results advocated their anti-cholinestrase and anti-oxidant activities. Treatment with TRZ-15 and TRZ-20 showed neuroprotective ability of the compounds as evidenced from the improved cognitive ability in the animals, and decrease in Abeta1-42 burden and cytochrome c and cleaved caspase-3 levels in the brain. This study also demonstrates positive involvement of the novel triazine derivatives in the Wnt/beta-catenin pathway. Immunoblot and immunofluorescence data suggested that ratio of pGSK3/GSK3 and beta-catenin got dramatically improved after treatment with TRZ-15 and TRZ-20. TRZ-15 and TRZ-20 showed neuroprotection in scopolamine-induced amnesic mice and Abeta1-42-induced Alzheimer's rat model and also activate the Wnt/beta-catenin signaling pathway. These findings conclude that TRZ-15 and TRZ-20 could be a therapeutic approach to treat AD.
ESTHER : Sinha_2015_Mol.Neurobiol_52_638
PubMedSearch : Sinha_2015_Mol.Neurobiol_52_638
PubMedID: 25257697

Title : Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol-3-phosphate-specific phospholipase A1 activity - Agarwal_2015_Nat.Commun_6_8745
Author(s) : Agarwal S , Kim H , Chan RB , Williamson R , Cho W , Paolo GD , Satchell KJ
Ref : Nat Commun , 6 :8745 , 2015
Abstract : Vibrio cholerae, responsible for acute gastroenteritis secretes a large multifunctional-autoprocessing repeat-in-toxin (MARTX) toxin linked to evasion of host immune system, facilitating colonization of small intestine. Unlike other effector domains of the multifunctional toxin that target cytoskeleton, the function of alpha-beta hydrolase (ABH) remained elusive. This study demonstrates that ABH is an esterase/lipase with catalytic Ser-His-Asp triad. ABH binds with high affinity to phosphatidylinositol-3-phosphate (PtdIns3P) and cleaves the fatty acid in PtdIns3P at the sn1 position in vitro making it the first PtdIns3P-specific phospholipase A1 (PLA1). Expression of ABH in vivo reduces intracellular PtdIns3P levels and its PtdIns3P-specific PLA1 activity blocks endosomal and autophagic pathways. In accordance with recent studies acknowledging the potential of extracellular pathogens to evade or exploit autophagy to prevent their clearance and facilitate survival, this is the first report highlighting the role of ABH in inhibiting autophagy and endosomal trafficking induced by extracellular V. cholerae.
ESTHER : Agarwal_2015_Nat.Commun_6_8745
PubMedSearch : Agarwal_2015_Nat.Commun_6_8745
PubMedID: 26498860
Gene_locus related to this paper: vibch-rtxAABH

Title : NGA- A Novel Hypothetical Drug Model: Combinatorial Approach to Multifactorial Alzheimer's Disease - Devi_2015_Curr.Drug.Discov.Technol_12_214
Author(s) : Devi CS , Mohanasrinivasan V , Priyadoss CG , Arora N , Singh B , Mittal N , Agarwal S , Saxena S
Ref : Curr Drug Discov Technol , 12 :214 , 2015
Abstract : Alzheimer's disease (AD) is supposed to stanch from inappropriate waving in the brain sections related to memory and perception. The incidence of AD in distressed person associated with an upsurge in the accumulation of amyloid plaque-rich senile plaques and neurofibrillary tangles in the brain. We hypothesize that a combination therapy provides a new treatment for AD. We propose that an anti-AD drug, NGA, a combination of NSAIDS, Galanthamine and ACS-40 may be useful in preventing the formation of amyloid plaques from beta-amyloid. Being a widespread incurable disease, the treatment for Alzheimer's has been at the forefront of the medical research work. We propose a novel drug-like NGA will allow for the effective control and treatment of the progression of AD by preventing acetylcholinesterase activity and reducing plaque formation that forms the distinctive symptom for the identification of the onset of AD. A combinatory use of NSAID with a natural neurotransmitter will allow for an efficient control of amyloid beta toxicity and will open doors for the treatment of a myriad of other neurodegenerative diseases.
ESTHER : Devi_2015_Curr.Drug.Discov.Technol_12_214
PubMedSearch : Devi_2015_Curr.Drug.Discov.Technol_12_214
PubMedID: 26572486

Title : Meta-analysis of the cardiovascular outcomes with dipeptidyl peptidase 4 inhibitors: validation of the current FDA mandate - Agarwal_2014_Am.J.Cardiovasc.Drugs_14_191
Author(s) : Agarwal S , Parashar A , Menon V
Ref : Am J Cardiovasc Drugs , 14 :191 , 2014
Abstract : BACKGROUND: Earlier meta-analyses have demonstrated a significant reduction in major adverse cardiovascular events (MACE) with dipeptidyl peptidase 4-inhibitor (DPPI) use, as compared with placebo or alternative anti-diabetic therapies. However, the large phase III/IV trials, namely SAVOR-TIMI 53 and the EXAMINE trials, failed to demonstrate any significant differences in MACE between DPPI and placebo. We aimed to perform an updated meta-analysis of randomized controlled trials (RCTs) to investigate the differences in cardiovascular death, myocardial infarction (MI), and stroke between DPPI and placebo/alternative agents.
METHODS: We searched the MEDLINE, EMBASE, and Cochrane databases for relevant phase III/IV RCTs. Unpublished trials with results available on national clinical trials registers were also included. RCTs with follow-up duration >/=24 weeks were included if they compared DPPI with placebo or an alternative anti-diabetic agent.
RESULTS: A total of 82 RCTs including 73,678 patients were included. We did not observe any significant difference in the pooled odds of cardiovascular death, MI, or stroke in the composite DPPI arm as compared with the control arm. Similarly, the pooled odds of all-cause death and MACE were statistically similar between the two groups. None of the clinical outcomes studied demonstrated evidence of statistical heterogeneity or publication bias. Due to a larger sample size and a longer duration of follow-up, both SAVOR-TIMI 53 and EXAMINE trials had a considerably larger contribution to the pooled estimates in our meta-analysis, driving the updated pooled estimates towards null for all clinical outcomes assessed.
CONCLUSIONS: DPPI use was not associated with increased incidence of cardiovascular mortality, MI, stroke, or MACE compared with placebo or alternative anti-diabetic agents.
ESTHER : Agarwal_2014_Am.J.Cardiovasc.Drugs_14_191
PubMedSearch : Agarwal_2014_Am.J.Cardiovasc.Drugs_14_191
PubMedID: 24687214

Title : Curcumin-Loaded Nanoparticles Potently Induce Adult Neurogenesis and Reverse Cognitive Deficits in Alzheimer's Disease Model via Canonical Wnt\/beta-Catenin Pathway - Tiwari_2014_ACS.Nano_8_76
Author(s) : Tiwari SK , Agarwal S , Seth B , Yadav A , Nair S , Bhatnagar P , Karmakar M , Kumari M , Chauhan LK , Patel DK , Srivastava V , Singh D , Gupta SK , Tripathi A , Chaturvedi RK , Gupta KC
Ref : ACS Nano , 8 :76 , 2014
Abstract : Neurogenesis, a process of generation of new neurons, is reported to be reduced in several neurodegenerative disorders including Alzheimer's disease (AD). Induction of neurogenesis by targeting endogenous neural stem cells (NSC) could be a promising therapeutic approach to such diseases by influencing the brain self-regenerative capacity. Curcumin, a neuroprotective agent, has poor brain bioavailability. Herein, we report that curcumin-encapsulated PLGA nanoparticles (Cur-PLGA-NPs) potently induce NSC proliferation and neuronal differentiation in vitro and in the hippocampus and subventricular zone of adult rats, as compared to uncoated bulk curcumin. Cur-PLGA-NPs induce neurogenesis by internalization into the hippocampal NSC. Cur-PLGA-NPs significantly increase expression of genes involved in cell proliferation (reelin, nestin, and Pax6) and neuronal differentiation (neurogenin, neuroD1, neuregulin, neuroligin, and Stat3). Curcumin nanoparticles increase neuronal differentiation by activating the Wnt/beta-catenin pathway, involved in regulation of neurogenesis. These nanoparticles caused enhanced nuclear translocation of beta-catenin, decreased GSK-3beta levels, and increased promoter activity of the TCF/LEF and cyclin-D1. Pharmacological and siRNA-mediated genetic inhibition of the Wnt pathway blocked neurogenesis-stimulating effects of curcumin. These nanoparticles reverse learning and memory impairments in an amyloid beta induced rat model of AD-like phenotypes, by inducing neurogenesis. In silico molecular docking studies suggest that curcumin interacts with Wif-1, Dkk, and GSK-3beta. These results suggest that curcumin nanoparticles induce adult neurogenesis through activation of the canonical Wnt/beta-catenin pathway and may offer a therapeutic approach to treating neurodegenerative diseases such as AD, by enhancing a brain self-repair mechanism.
ESTHER : Tiwari_2014_ACS.Nano_8_76
PubMedSearch : Tiwari_2014_ACS.Nano_8_76
PubMedID: 24467380

Title : Protective effects of Moringa oleifera Lam. leaves against arsenic-induced toxicity in mice - Sheikh_2014_Asian.Pac.J.Trop.Biomed_4_S353
Author(s) : Sheikh A , Yeasmin F , Agarwal S , Rahman M , Islam K , Hossain E , Hossain S , Karim MR , Nikkon F , Saud ZA , Hossain K
Ref : Asian Pac J Trop Biomed , 4 :S353 , 2014
Abstract : OBJECTIVE: To evaluate the protective role of leaves of Moringa oleifera (M. oleifera) Lam. against arsenic-induced toxicity in mice.
METHODS: Swiss albino male mice were divided into four groups. The first group was used as non-treated control group while, the second, third, and fourth groups were treated with M. oleifera leaves (50 mg/kg body weight per day), sodium arsenite (10 mg/kg body weight per day) and sodium arsenite plus M. oleifera leaves, respectively. Serum indices related to cardiac, liver and renal functions were analyzed to evaluate the protective effect of Moringa leaves on arsenic-induced effects in mice.
RESULTS: It revealed that food supplementation of M. oleifera leaves abrogated the arsenic-induced elevation of triglyceride, glucose, urea and the activities of alkaline phospatase, aspartate aminotransferase and alanine aminotransferase in serum. M. oleifera leaves also prevented the arsenic-induced perturbation of serum butyryl cholinesterase activity, total cholesterol and high density lipoprotein cholesterol.
CONCLUSIONS: The results indicate that the leaves of M. oleifera may be useful in reducing the effects of arsenic-induced toxicity.
ESTHER : Sheikh_2014_Asian.Pac.J.Trop.Biomed_4_S353
PubMedSearch : Sheikh_2014_Asian.Pac.J.Trop.Biomed_4_S353
PubMedID: 25183111

Title : Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum - Solyakov_2011_Nat.Commun_2_565
Author(s) : Solyakov L , Halbert J , Alam MM , Semblat JP , Dorin-Semblat D , Reininger L , Bottrill AR , Mistry S , Abdi A , Fennell C , Holland Z , Demarta C , Bouza Y , Sicard A , Nivez MP , Eschenlauer S , Lama T , Thomas DC , Sharma P , Agarwal S , Kern S , Pradel G , Graciotti M , Tobin AB , Doerig C
Ref : Nat Commun , 2 :565 , 2011
Abstract : The role of protein phosphorylation in the life cycle of malaria parasites is slowly emerging. Here we combine global phospho-proteomic analysis with kinome-wide reverse genetics to assess the importance of protein phosphorylation in Plasmodium falciparum asexual proliferation. We identify 1177 phosphorylation sites on 650 parasite proteins that are involved in a wide range of general cellular activities such as DNA synthesis, transcription and metabolism as well as key parasite processes such as invasion and cyto-adherence. Several parasite protein kinases are themselves phosphorylated on putative regulatory residues, including tyrosines in the activation loop of PfGSK3 and PfCLK3; we show that phosphorylation of PfCLK3 Y526 is essential for full kinase activity. A kinome-wide reverse genetics strategy identified 36 parasite kinases as likely essential for erythrocytic schizogony. These studies not only reveal processes that are regulated by protein phosphorylation, but also define potential anti-malarial drug targets within the parasite kinome.
ESTHER : Solyakov_2011_Nat.Commun_2_565
PubMedSearch : Solyakov_2011_Nat.Commun_2_565
PubMedID: 22127061

Title : Pharmacokinetics and bioequivalence study of a fixed dose combination of rabeprazole and itopride in healthy Indian volunteers - Sahoo_2009_Arzneimittelforschung_59_451
Author(s) : Sahoo BK , Das A , Agarwal S , Bhaumik U , Bose A , Ghosh D , Roy B , Pal TK
Ref : Arzneimittelforschung , 59 :451 , 2009
Abstract : The aim of the present study was to compare the pharmacokinetics of rabeprazole (CAS 117976-89-3) and itopride (CAS 122898-67-3) after oral administration of a rabeprazole (20 mg)-itopride (150 mg) fixed dose combination (FDC) in healthy human volunteers. The bioequivalence of two formulations (test and reference) was determined in 12 healthy Indian male volunteers (age: 25.25 +/- 4.69 years; weight: 60.50 +/- 5.04 kg) in a randomized, single-dose, two-period, two-treatment crossover study. Both formulations were administered orally as a single dose, with the treatments separated by a washout period of 1 week. Rabeprazole and itopride plasma levels were determined by a validated HPLC method using UV detection. The formulations were compared using the pharmacokinetic parameters area under the plasma concentration-time curve (AUC(0-t)), area under the plasma concentration-time curve from zero to infinity (AUC(0-infinity)) and peak plasma concentration (Cmax). General linear model (GLM) procedures were used in which sources of variation were subject, treatment and period. The results indicated that there were no statistically significant differences (P > 0.05) between the logarithmically transformed AUC(0-infinity) and Cmax values between test and reference formulation. The 90% confidence interval for the ratio of the logarithmically transformed AUC(0-t), AUC(0-infinity) and Cmax were within the bioequivalence limits of 0.8-1.25 and the relative bioavailability of rabeprazole and itopride test and reference formulations was 98.24 and 93.65%, respectively.
ESTHER : Sahoo_2009_Arzneimittelforschung_59_451
PubMedSearch : Sahoo_2009_Arzneimittelforschung_59_451
PubMedID: 19856792

Title : Subcellular localization of the yeast proteome - Kumar_2002_Genes.Dev_16_707
Author(s) : Kumar A , Agarwal S , Heyman JA , Matson S , Heidtman M , Piccirillo S , Umansky L , Drawid A , Jansen R , Liu Y , Cheung KH , Miller P , Gerstein M , Roeder GS , Snyder M
Ref : Genes Dev , 16 :707 , 2002
Abstract : Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http:\/\/ygac.med.yale.edu.
ESTHER : Kumar_2002_Genes.Dev_16_707
PubMedSearch : Kumar_2002_Genes.Dev_16_707
PubMedID: 11914276
Gene_locus related to this paper: yeast-mgll

Title : Cyclic, Selectively Permeable Acetylcholinesterase Inhibitors -
Author(s) : Mallender WD , Agarwal S , Ma W , Spatola AF , Rosenberry TL
Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :245 , 1998
PubMedID:

Title : Serum enzyme abnormalities in protein energy malnutrition - Kumari_1993_Ind.Pediat_30_469
Author(s) : Kumari R , Rao YN , Talukdar B , Agarwal S , Puri RK
Ref : Indian Pediatrics , 30 :469 , 1993
Abstract : Six serum enzymes, alkaline phosphatase, cholinesterase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyl transpeptidase were studied in 30 cases of protein energy malnutrition (PEM). The mean serum values of alkaline phosphatase, cholinesterase and lactate dehydrogenase in cases of PEM were significantly lower than the controls, lowering being maximum in PEM Grade IV. The mean serum values of aspartate aminotransferase and alanine aminotransferase in patients with PEM were significantly higher than the controls. The mean serum values of gamma-glutamyl transpeptidase showed similar significant rise in all but PEM Grade IV. The degree of increase in the serum values of these three enzymes were maximum in cases with PEM Grade I. These findings suggest that abnormalities in blood levels of these enzymes occur in any form of PEM and these are related to the severity of the disease.
ESTHER : Kumari_1993_Ind.Pediat_30_469
PubMedSearch : Kumari_1993_Ind.Pediat_30_469
PubMedID: 8288327