Kim T

References (8)

Title : Systematic review and meta-analysis of the genetics of peripheral arterial disease - Ochoa_2024_JVS.Vasc.Sci_5_100133
Author(s) : Ochoa Chaar CI , Kim T , Alameddine D , Dewan A , Guzman R , Dardik A , Grossetta Nardini HK , Wallach JD , Kullo I , Murray M
Ref : JVS Vasc Sci , 5 :100133 , 2024
Abstract : BACKGROUND: Peripheral artery disease (PAD) impacts more than 200 million people worldwide. The understanding of the genetics of the disease and its clinical implications continue to evolve. This systematic review provides a comprehensive summary of all DNA variants that have been studied in association with the diagnosis and progression of PAD, with a meta-analysis of the ones replicated in the literature. METHODS: A systematic review of all studies examining DNA variants associated with the diagnosis and progression of PAD was performed. Candidate gene and genome-wide association studies (GWAS) were included. A meta-analysis of 13 variants derived from earlier smaller candidate gene studies of the diagnosis of PAD was performed. The literature on the progression of PAD was limited, and a meta-analysis was not feasible because of the heterogeneity in the criteria used to characterize it. RESULTS: A total of 231 DNA variants in 112 papers were studied for the association with the diagnosis of PAD. There were significant variations in the definition of PAD and the selection of controls in the various studies. GWAS have established 19 variants associated with the diagnosis of PAD that were replicated in several large patient cohorts. Only variants in intercellular adhesion molecule-1 (rs5498), IL-6 (rs1800795), and hepatic lipase (rs2070895) showed significant association with the diagnosis of PAD. However, these variants were not noted in the published GWAS. CONCLUSIONS: Genetic research in the diagnosis of PAD has significant heterogeneity, but recent GWAS have demonstrated variants consistently associated with the disease. More research focusing on the progression of PAD is needed to identify patients at risk of adverse events and develop strategies that would improve their outcomes.
ESTHER : Ochoa_2024_JVS.Vasc.Sci_5_100133
PubMedSearch : Ochoa_2024_JVS.Vasc.Sci_5_100133
PubMedID: 38314202

Title : Cognitive decline according to amyloid uptake in patients with poststroke cognitive impairment - Yoon_2021_Medicine.(Baltimore)_100_e27252
Author(s) : Yoon B , Yang DW , Hong YJ , Kim T , Na S , Noh SM , Park HL , Ku BD , Yang YS , Choi H , Jang JW , Kim S , Kim Y , Shim Y
Ref : Medicine (Baltimore) , 100 :e27252 , 2021
Abstract : BACKGROUND AND PURPOSE: Poststroke cognitive impairment (PSCI) is common, but the impact of beta-amyloid (Abeta) on PSCI is uncertain. The proposed study will investigate amyloid pathology in participants with PSCI and how differently their cognition progress according to the amyloid pathology. METHODS: This multicenter study was designed to be prospective and observational based on a projected cohort size of 196 participants with either newly developed cognitive impairment, or rapidly aggravated CI, within 3 months after acute cerebral infarction. They will undergo 18F-flutemetamol positron emission tomography at baseline and will be categorized as either amyloid-positive (A+) or amyloid-negative (A-) by visual rating. The primary outcome measures will be based on Korean Mini-Mental State Examination changes (baseline to 12months) between the A+ and A- groups. The secondary outcome measures will be the dementia-conversion rate and changes in the Korean version of the Montreal Cognitive Assessment (baseline to 12months) between the A+ and A- groups. CONCLUSIONS: This study will provide a broadened perspective on the impact of Abeta on the cause and outcomes of PSCI in clinical practice. Identifying amyloid pathology in patients with PSCI will help select patients who need more focused treatments such as acetylcholinesterase inhibitors. TRIAL REGISTRATION: Clinical Research Information Service identifier: KCT0005086.
ESTHER : Yoon_2021_Medicine.(Baltimore)_100_e27252
PubMedSearch : Yoon_2021_Medicine.(Baltimore)_100_e27252
PubMedID: 34559128

Title : Decomposition of the PET Film by MHETase Using Exo-PETase Function - Sagong_2020_ACS.Catal_10_48050
Author(s) : Sagong HY , Seo H , Kim T , Son H , Joo S , Lee S , Kim S , Woo JS , Hwang S , Kim KJ
Ref : ACS Catal , 10 :4805 , 2020
Abstract : Monohydroxyethyl terephthalate (MHET) hydrolase (MHETase) is an enzyme known to be involved in the final degradation step of poly(ethylene terephthalate) (PET) by hydrolyzing MHET into terephthalic acid and ethylene glycol in Ideonella sakaiensis. Here, we report the extracellular production of MHETase in an active form with a proper folding. Based on the structural observations and biochemical experiments, we reveal that MHETase also functions as exo-PETase by hydrolyzing the synthesized PET pentamer. We further present that MHETase has a hydrolysis activity against the termini-generated PET film, demonstrating the exo-PETase function of the enzyme. We also develop a MHETase R411K/S416A/F424I variant with a higher BHET activity, and the variant exhibits an enhanced degradation activity against the PET film. Based on these results, we propose that MHETase plays several roles in the biodegradation of PET using the BHETase and exo-PETase activities as well as the MHET hydrolysis function
ESTHER : Sagong_2020_ACS.Catal_10_48050
PubMedSearch : Sagong_2020_ACS.Catal_10_48050
PubMedID:
Gene_locus related to this paper: idesa-mheth

Title : Deastringent Peel Extracts of Persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) Protect Neuronal PC-12 and SH-SY5Y Cells against Oxidative Stress - Jeong_2018_J.Microbiol.Biotechnol_28_1094
Author(s) : Jeong DW , Cho CH , Lee JS , Lee SH , Kim T , Kim DO
Ref : J Microbiol Biotechnol , 28 :1094 , 2018
Abstract : The peel of astringent persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) is a by-product of dried persimmon (gotgam). We investigated if deastringent peel extracts of persimmon cv. Cheongdo-Bansi had antioxidative and neuroprotective properties. Two different extracts were prepared: thermally and nonthermally treated persimmon peel extracts (TPE and NTPE, respectively). Both TPE and NTPE were fractionated sequentially in n-hexane, chloroform, ethyl acetate, n-butanol, and water. The TPE and NTPE ethyl acetate fractions had the highest total phenolic and flavonoid contents as well as antioxidant capacities among all the fractions. Pretreatment of neuronal PC-12 and SH-SY5Y cells with the TPE and NTPE ethyl acetate fractions increased cell viability after exposure to oxidative stress. The ethyl acetate fraction of TPE attenuated oxidative stress inside both PC-12 and SH-SY5Y cells more effectively than that of NTPE. Furthermore, the TPE and NTPE ethyl acetate fractions inhibited acetylcholinesterase and butyrylcholinesterase. Analysis of ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry results revealed gallic acid, kaempferol, kaempferol-3-O-galactoside, kaempferol-3-O-glucoside, quercetin, quercetin-3-O-galactoside, quercetin-3-O-galactoside-2'-O-gallate, and quercetin-3-O-glucoside as the major phenolics of the TPE and NTPE ethyl acetate fractions. Taken together, these results suggest that the ethyl acetate fraction of deastringent persimmon peel is rich in antioxidants and has potential as a functional food to reduce oxidative stress.
ESTHER : Jeong_2018_J.Microbiol.Biotechnol_28_1094
PubMedSearch : Jeong_2018_J.Microbiol.Biotechnol_28_1094
PubMedID: 29975999

Title : In Silico, In Vitro, and In Vivo Studies Indicate the Potential Use of Bolaamphiphiles for Therapeutic siRNAs Delivery - Kim_2013_Mol.Ther.Nucleic.Acids_2_e80
Author(s) : Kim T , Afonin KA , Viard M , Koyfman AY , Sparks S , Heldman E , Grinberg S , Linder C , Blumenthal RP , Shapiro BA
Ref : Mol Ther Nucleic Acids , 2 :e80 , 2013
Abstract : Specific small interfering RNAs (siRNAs) designed to silence different oncogenic pathways can be used for cancer therapy. However, non-modified naked siRNAs have short half-lives in blood serum and encounter difficulties in crossing biological membranes due to their negative charge. These obstacles can be overcome by using siRNAs complexed with bolaamphiphiles, consisting of two positively charged head groups that flank an internal hydrophobic chain. Bolaamphiphiles have relatively low toxicities, long persistence in the blood stream, and most importantly, in aqueous conditions can form poly-cationic micelles thus, becoming amenable to association with siRNAs. Herein, two different bolaamphiphiles with acetylcholine head groups attached to an alkyl chain in two distinct configurations are compared for their abilities to complex with siRNAs and deliver them into cells inducing gene silencing. Our explicit solvent molecular dynamics (MD) simulations showed that bolaamphiphiles associate with siRNAs due to electrostatic, hydrogen bonding, and hydrophobic interactions. These in silico studies are supported by various in vitro and in cell culture experimental techniques as well as by some in vivo studies. Results demonstrate that depending on the application, the extent of siRNA chemical protection, delivery efficiency, and further intracellular release can be varied by simply changing the type of bolaamphiphile used.Molecular Therapy-Nucleic Acids (2013) 2, e80; doi:10.1038/mtna.2013.5; published online 19 March 2013.
ESTHER : Kim_2013_Mol.Ther.Nucleic.Acids_2_e80
PubMedSearch : Kim_2013_Mol.Ther.Nucleic.Acids_2_e80
PubMedID: 23511334

Title : Prenatally detected congenital perineal mass using 3D ultrasound which was diagnosed as lipoblastoma combined with anorectal malformation: case report - Ahn_2010_J.Korean.Med.Sci_25_1093
Author(s) : Ahn KH , Boo YJ , Seol HJ , Park HT , Hong SC , Oh MJ , Kim T , Kim HJ , Kim YT , Kim SH , Lee KW
Ref : J Korean Med Sci , 25 :1093 , 2010
Abstract : We report a case of prenatally diagnosed congenital perineal mass which was combined with anorectal malformation. The mass was successfully treated with posterior sagittal anorectoplasty postnatally. On ultrasound examination at a gestational age of 23 weeks the fetal perineal mass were found on the right side. Any other defects were not visible on ultrasonography during whole gestation. Amniocentesis was performed to evaluate the fetal karyotyping and acetylcholinesterase which were also normal. As the fetus grew up, the mass size was slowly increased more and more. At birth, a female neonate had a perineal mass on the right side as expected. During operation, the anal sphincteric displacement was found near the mass and reconstructed through posterior sagittal incision. This is the first reported case of prenatally diagnosed congenital perineal mass, after birth which was diagnosed as lipoblastoma and even combined with anorectal malformation. This case shows that it can be of clinical importance to be aware of this rare fetal perineal mass in prenatal diagnosis and counseling.
ESTHER : Ahn_2010_J.Korean.Med.Sci_25_1093
PubMedSearch : Ahn_2010_J.Korean.Med.Sci_25_1093
PubMedID: 20592907

Title : Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation - Tingley_2007_Proc.Natl.Acad.Sci.U.S.A_104_8461
Author(s) : Tingley WG , Pawlikowska L , Zaroff JG , Kim T , Nguyen T , Young SG , Vranizan K , Kwok PY , Whooley MA , Conklin BR
Ref : Proc Natl Acad Sci U S A , 104 :8461 , 2007
Abstract : Sudden cardiac death due to abnormal heart rhythm kills 400,000-460,000 Americans each year. To identify genes that regulate heart rhythm, we are developing a screen that uses mouse embryonic stem cells (mESCs) with gene disruptions that can be differentiated into cardiac cells for phenotyping. Here, we show that the heterozygous disruption of the Akap10 (D-AKAP2) gene that disrupts the final 51 aa increases the contractile response of cultured cardiac cells to cholinergic signals. In both heterozygous and homozygous mutant mice derived from these mESCs, the same Akap10 disruption increases the cardiac response to cholinergic signals, suggesting a dominant interfering effect of the Akap10 mutant allele. The mutant mice have cardiac arrhythmias and die prematurely. We also found that a common variant of AKAP10 in humans (646V, 40% of alleles) was associated with increased basal heart rate and decreased heart rate variability (markers of low cholinergic/vagus nerve sensitivity). These markers predict an increased risk of sudden cardiac death. Although the molecular mechanism remains unknown, our findings in mutant mESCs, mice, and a common human AKAP10 SNP all suggest a role for AKAP10 in heart rhythm control. Our stem cell-based screen may provide a means of identifying other genes that control heart rhythm.
ESTHER : Tingley_2007_Proc.Natl.Acad.Sci.U.S.A_104_8461
PubMedSearch : Tingley_2007_Proc.Natl.Acad.Sci.U.S.A_104_8461
PubMedID: 17485678

Title : Effects of mobile buffers on facilitation: experimental and computational studies - Tang_2000_Biophys.J_78_2735
Author(s) : Tang Y , Schlumpberger T , Kim T , Lueker M , Zucker RS
Ref : Biophysical Journal , 78 :2735 , 2000
Abstract : Facilitation is an important form of short-term plasticity that occurs in most synapses. At crayfish neuromuscular junctions, basal transmission and facilitation were significantly reduced after presynaptic introduction of "fast" high-affinity calcium buffers, and the decay of facilitation was accelerated. The existence of residual calcium during facilitation was also demonstrated. Computational modeling of three-dimensional buffered Ca(2+) diffusion and binding to secretory and facilitation targets suggest that the facilitation site is located away from a secretory trigger mediating exocytosis; otherwise, the facilitation site would be saturated by each action potential. Our simulations account for many characteristics of facilitation and effects of exogenous buffer, and suggest that facilitation is caused by residual calcium gaining access to a site distinct from the secretory trigger through restricted diffusion.
ESTHER : Tang_2000_Biophys.J_78_2735
PubMedSearch : Tang_2000_Biophys.J_78_2735
PubMedID: 10827959