Kushwaha M

References (2)

Title : Biodegradation of di-n-butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway - Mahajan_2019_Int.J.Biol.Macromol_122_806
Author(s) : Mahajan R , Verma S , Kushwaha M , Singh D , Akhter Y , Chatterjee S
Ref : Int J Biol Macromol , 122 :806 , 2019
Abstract : A priority pollutant Phthalate Esters (PAEs) are widely used as plasticizers and are responsible mainly for carcinogenicity and endocrine disruption in human. For the bioremediation of PAEs, a psychrotolerant Sphingobium yanoikuyae strain P4, capable of utilizing many phthalates dimethyl phthalate (DMP), diethyl phthalate (DEP), dinbutyl phthalate (DBP), diisobutyl phthalate (DIBP), butyl benzyl phthalate (BBP), and few Polycyclic Aromatic Hydrocarbons as the sole source of carbon and energy was isolated from Palampur, Kangra, Himachal Pradesh, India. 100% utilization of DBP (1gL(-1)) by the strain was observed within 24h of incubation at 28 degrees C. Interestingly the strain also degraded DBP completely at 20 degrees C and 15 degrees C within 36h and 60h, respectively. Esterase involved in DBP degradation was found to be inducible in nature and intracellular. Comparative sequence analysis of carboxylesterase enzyme sequences revealed conserved motifs: G-X-S-X-G and -HGG- which were the characteristic peptide motifs reported in different esterases. Structural analysis showed that the enzyme belongs to serine hydrolase superfamily, which has an alpha/beta hydrolase fold. Interaction and binding of DBP to a catalytic Ser(184) residue in the esterase enzyme were also analysed. In conclusion, carboxylesterase possess the required active site which may be involved in the catabolism of DBP.
ESTHER : Mahajan_2019_Int.J.Biol.Macromol_122_806
PubMedSearch : Mahajan_2019_Int.J.Biol.Macromol_122_806
PubMedID: 30395899
Gene_locus related to this paper: sphya-k9da91

Title : Profenofos, an Acetylcholinesterase-Inhibiting Organophosphorus Pesticide: A Short Review of Its Usage, Toxicity, and Biodegradation - Kushwaha_2016_J.Environ.Qual_45_1478
Author(s) : Kushwaha M , Verma S , Chatterjee S
Ref : J Environ Qual , 45 :1478 , 2016
Abstract : Pesticides play an important role in the protection of different crops. Among the diverse sets of pesticides used all over the world, the organophosphates are the most widely used group. Profenofos [O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate] is one of the most largely used organophosphate insecticides on field crops, vegetables, and fruit crops. The World Health Organization classifies this compound as moderately hazardous (Toxicity Class II), and its residues have been found in vegetables like okra [ (L.) Moench], gooseberries ( sp.), green chilies [ (L.)], curry leaves [ (L.) Spreng], mint leaves [ (L.)], and coriander leaves [ (L.)]. Dietary intake of profenofos (PFF) is the major exposure pathway for humans. When applied to agricultural fields, PFF residues spread into every part of the environment: ambient air, surface water, and soil. In this review, we discuss the worldwide usage of PFF pesticide, its toxic effects on humans and other living organisms in the environment, and biodegradation of this chemical by various microbial strains. To date, no complete biodegradation pathway has been established for PFF pesticide, calling for a study of this nature.
ESTHER : Kushwaha_2016_J.Environ.Qual_45_1478
PubMedSearch : Kushwaha_2016_J.Environ.Qual_45_1478
PubMedID: 27695768