Chatterjee S

References (20)

Title : Cumulative Anticholinergic Burden and its Predictors among Older Adults with Alzheimer's Disease Initiating Cholinesterase Inhibitors - Talwar_2024_Drugs.Aging__
Author(s) : Talwar A , Chatterjee S , Sherer J , Abughosh S , Johnson M , Aparasu RR
Ref : Drugs & Aging , : , 2024
Abstract : BACKGROUND: Cumulative anticholinergic burden refers to the cumulative effect of multiple medications with anticholinergic properties. However, concomitant use of cholinesterase inhibitors (ChEIs) and anticholinergic burden can nullify the benefit of the treatment and worsen Alzheimer's disease (AD). A literature gap exists regarding the extent of the cumulative anticholinergic burden and associated risk factors in AD. Therefore, this study evaluated the prevalence and predictors of cumulative anticholinergic burden among patients with AD initiating ChEIs. METHODS: A retrospective longitudinal cohort study was conducted using the Medicare claims data involving parts A, B, and D from 2013 to 2017. The study sample included older adults (65 years and older) diagnosed with AD and initiating ChEIs (donepezil, rivastigmine, or galantamine). The cumulative anticholinergic burden was calculated based on the Anticholinergic Cognitive Burden scale and patient-specific dosing using the defined daily dose over the 1 year follow-up period after ChEI initiation. Incremental anticholinergic burden levels were dichotomized into moderate-high (sum of standardized daily anticholinergic exposure over a year (TSDD) score <= 90) versus low-no (score 0-89). The Andersen Behavioral Model was used as the conceptual framework for selecting the predictors under the predisposing, enabling, and need categories. A multivariable logistic regression model was used to evaluate the predictors of high-moderate versus low-no cumulative anticholinergic burden. A multinomial logistic regression model was also used to determine the factors associated with patients having moderate and high burdens compared to low/no burdens. RESULTS: The study included 222,064 older adults with AD with incident ChEI use (mean age 82.24 +/- 7.29, 68.9% females, 83.6% White). Overall, 80.48% had some anticholinergic burden during the follow-up, with 36.26% patients with moderate (TSDD scores 90-499), followed by 24.76% high (TSDD score > 500), and 19.46% with low (TSDD score 1-89) burden categories. Predisposing factors such as age; African American, Asian, or Hispanic race; and need factors included comorbidities such as dyslipidemia, syncope, delirium, fracture, pneumonia, epilepsy, and claims-based frailty index were less likely to be associated with the moderate-high anticholinergic burden. The factors that increased the odds of moderate-high burden were predisposing factors such as female sex; enabling factors such as dual eligibility and diagnosis year; and need factors such as baseline burden, behavioral and psychological symptoms of dementia, depression, insomnia, urinary incontinence, irritable bowel syndrome, anxiety, muscle spasm, gastroesophageal reflux disease, heart failure, and dysrhythmia. Most of these findings remained consistent with multinomial logistic regression. CONCLUSION: Four out of five older adults with AD had some level of anticholinergic burden, with over 60% having moderate-high anticholinergic burden. Several predisposing, enabling, and need factors were associated with the cumulative anticholinergic burden. The study findings suggest a critical need to minimize the cumulative anticholinergic burden to improve AD care.
ESTHER : Talwar_2024_Drugs.Aging__
PubMedSearch : Talwar_2024_Drugs.Aging__
PubMedID: 38467994

Title : Isolation, Characterization, and In Silico Interaction Studies of Bioactive Compounds from Caesalpinia bonducella with Target Proteins Involved in Alzheimer's Disease - Khoba_2022_Appl.Biochem.Biotechnol__
Author(s) : Khoba K , Kumar S , Chatterjee S , Purty RS
Ref : Appl Biochem Biotechnol , : , 2022
Abstract : Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder characterized by memory loss, cognitive deterioration, and neuropsychiatric symptoms. Various drug targets implicated in AD are amyloid beta peptides, cholinesterase enzymes, and anti-amylogenic protein. Medicinal plants derived phytochemical constituents provide a vast pool of diverse compounds as a source of novel drugs. In view of this, the Caesalpinia bonducella seed extract and its active phytoconstituents were used to study the disease-modifying effects in Alzheimer's disease. The present study successfully demonstrated the therapeutic potential of various phytochemicals as it binds to multiple drug targets, resulting in inhibition of acetylcholinesterase (AChE) enzyme, butyrylcholinesterase (BuChE), BACE-1 enzyme, and anti-amylogenic protein as indicated by docking analysis. In conclusion, phytochemicals identified can be used as a suitable lead to developing a molecule that might have multi-targeted directed ligand (MTDL) potential and disease amelioration effects in Alzheimer's disease.
ESTHER : Khoba_2022_Appl.Biochem.Biotechnol__
PubMedSearch : Khoba_2022_Appl.Biochem.Biotechnol__
PubMedID: 35507252

Title : Risk of Serious Adverse Events Associated With Individual Cholinesterase Inhibitors Use in Older Adults With Dementia: A Population-Based Cohort Study - Masurkar_2022_Drugs.Aging__
Author(s) : Masurkar PP , Chatterjee S , Sherer JT , Chen H , Johnson ML , Aparasu RR
Ref : Drugs & Aging , : , 2022
Abstract : BACKGROUND AND OBJECTIVE: Cholinesterase inhibitors (ChEIs) are used as first-line pharmacotherapy to manage dementia. However, there are limited data regarding their relative safety. This study evaluated the risk of serious adverse events (SAEs) associated with individual ChEIs in older adults with dementia and also examined sex-based and dose-based effects on this risk. METHODS: This was a retrospective cohort study using 2013-2015 US Medicare claims data involving Parts A, B, and D. Patients aged <= 65 years with a dementia diagnosis and incident use of the ChEIs, namely donepezil, galantamine, or rivastigmine, were included. The primary outcome of interest was SAEs defined as emergency department visits, inpatient hospitalizations, or death within 6 months of ChEI initiation. Multivariable Cox proportional hazards regression with propensity score (PS) as a covariate and inverse probability of treatment weighting generated using generalized boosted models was used to assess the risk of SAEs across individual ChEIs. RESULTS: The study included 767,684 older adults with dementia who were incident new users of ChEIs (donepezil 79.42%, rivastigmine 17.67%, galantamine 2.91%). SAEs were observed in 15.5% of the cohort within 6 months of ChEI prescription. Cox regression model with PS as covariate found that patients prescribed rivastigmine (adjusted hazard ratio [aHR] 1.12; 95% CI 1.03-1.33) and galantamine (aHR 1.51; 95% CI 1.24-1.84) were at increased risk of SAEs compared with patients on donepezil. Stratified analyses revealed that rivastigmine was associated with an 18% increased risk for SAEs in females (aHR 1.18; 95% CI 1.06-1.31), and galantamine was associated with a 71% increased risk in males (aHR 1.71; 95% CI 1.17-2.51) compared with donepezil. High and recommended index doses of rivastigmine and galantamine were associated with an increased risk of SAEs compared with donepezil. The findings were consistent in sensitivity analyses. CONCLUSION: The study found that the risk of SAEs varied across individual ChEIs, with sex and dose moderating these effects. Therefore, these moderating effects should be carefully considered in personalizing dementia care.
ESTHER : Masurkar_2022_Drugs.Aging__
PubMedSearch : Masurkar_2022_Drugs.Aging__
PubMedID: 35666463

Title : Antimuscarinic Cascade Across Individual Cholinesterase Inhibitors in Older Adults with Dementia - Masurkar_2021_Drugs.Aging__
Author(s) : Masurkar PP , Chatterjee S , Sherer JT , Aparasu RR
Ref : Drugs & Aging , : , 2021
Abstract : BACKGROUND: Acetylcholinesterase inhibitors (AChEIs) have been associated with an increased risk of starting antimuscarinic treatment to treat overactive bladder (OAB)-an example of a prescribing cascade. Limited comparative data exist regarding the prescribing cascade of antimuscarinics across individual AChEIs in older adults with dementia. OBJECTIVE: This study examined the association between individual AChEI use and antimuscarinic cascade in older adults with dementia. METHODS: We conducted a new user retrospective cohort study from January 2005 to December 2018 using data from the TriNetX electronic medical record database, a federated electronic medical records network in the US. The cohort included patients 65 years or older with a diagnosis of dementia using AChEIs (donepezil, galantamine, or rivastigmine). Individual AChEIs were identified with index dates from 1 January 2006 to 31 June 2018, with a 1-year washout period. The study excluded patients with any antimuscarinic use and OAB diagnosis 1 year before the AChEI index date. The primary outcome of interest was the prescription of antimuscarinics within 6 months of the AChEI index date. A Cox proportional hazard model was used to assess the association between individual incident AChEI use and antimuscarinic prescribing cascade after controlling for several covariates. RESULTS: The study included 47,059 older adults with dementia who were incident users of AChEIs. Most of these patients were initiated with donepezil (83.1%), followed by rivastigmine (12.3%) and galantamine (4.6%). Overall, 8.16% of the study cohort had incident OAB diagnosis or antimuscarinic prescription. Antimuscarinics were initiated by 1725 (3.7%) older adults with dementia within 6 months of AChEI prescription, and cascade varied widely across individual agents-donepezil (3.9%), rivastigmine (2.6%), and galantamine (2.9%). Cox proportional hazard analyses revealed that donepezil users had an increased risk of receiving antimuscarinics (adjusted hazard ratio 1.55, 95% confidence interval 1.31-1.83) compared with rivastigmine. The findings were consistent in sensitivity analyses. CONCLUSION: This study found that donepezil use is more likely to lead to antimuscarinic cascade than rivastigmine. Future studies are needed to determine the potential consequences of this cascade in dementia.
ESTHER : Masurkar_2021_Drugs.Aging__
PubMedSearch : Masurkar_2021_Drugs.Aging__
PubMedID: 34027602

Title : lambda cyhalothrin induced toxicity and potential attenuation of hematological, biochemical, enzymological and stress biomarkers in Cyprinus carpio L. at environmentally relevant concentrations: A multiple biomarker approach - Chatterjee_2021_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_250_109164
Author(s) : Chatterjee A , Bhattacharya R , Chatterjee S , Saha NC
Ref : Comparative Biochemistry & Physiology C Toxicol Pharmacol , 250 :109164 , 2021
Abstract : The present study was aimed to evaluate the toxic effects of a commonly used synthetic pyrethroid, lambda cyhalothrin on the common carp, Cyprinus carpio L. The results depicted that 96 h LC(50) value of lambda cyhalothrin to the fish was 1.48 microg l(-1). During 45 days of chronic exposure a significant reduction (p < 0.05) in the RBC, hemoglobin, and hematocrit value of fish was observed in lambda cyhalothrin treated fish. Blood glucose, cholesterol and creatinine levels increased significantly, while total protein and albumin were significantly decreased (p < 0.05) in the exposed fish. Moreover, alanine aminotransferase and aspartate aminotransferase levels in the blood also increased significantly (p < 0.05) in the treated fish. In gills and liver, glutathione S-transferase (GST) and glutathione peroxidase (GPx) and in liver GST exhibited a significant initial augmentation followed by a subsequent reduction while catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) level increased markedly with incrementing concentrations of lambda cyhalothrin in both the organs. Acetylcholinesterase (AchE) activity in both gills and liver decreased in exposed fish upon addition lambda cyhalothrin. However, the hazardous effects of lambda cyhalothrin on C. carpio were characterized and portrayed by the development of integrated biomarker response (IBR), and biomarker response index (BRI). GUTS-SD and IT modeling were implied for a better interpretation of the toxicity. These results indicate that exposure to lambda cyhalothrin alters the survivability at the acute level and the activity of hematological, plasma biochemical as well as enzymological and stress parameters (in gills and liver) at the sublethal level in C. carpio.
ESTHER : Chatterjee_2021_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_250_109164
PubMedSearch : Chatterjee_2021_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_250_109164
PubMedID: 34390845

Title : Risk of overactive bladder associated with cholinesterase inhibitors in dementia - Masurkar_2021_J.Am.Geriatr.Soc__
Author(s) : Masurkar PP , Chatterjee S , Sherer JT , Chen H , Johnson ML , Aparasu RR
Ref : J Am Geriatr Soc , : , 2021
Abstract : BACKGROUND: Although cholinesterase inhibitors (ChEIs) are the primary treatment for dementia, they are associated with overactive bladder (OAB), necessitating antimuscarinic use. Limited data exist regarding the risk of OAB across individual ChEIs in dementia. This study evaluated the risk of OAB associated with individual ChEIs in older adults with dementia. METHODS: This was a new user retrospective cohort study using Medicare claims data involving Parts A, B, and D claims dataset from 2013 to 2015. The study included older adults (aged 65 and older) with a diagnosis of dementia using donepezil, galantamine, or rivastigmine. New ChEI claims were identified with a 6-month baseline washout period. Patients with OAB diagnosis or any antimuscarinic or mirabegron use in the baseline period were excluded. The primary outcome of interest was OAB diagnosis or prescription of antimuscarinics or mirabegron within 6 months of ChEI initiation. Multivariable cox proportional hazards regression with propensity scores (PS) as covariates and inverse probability of treatment weighting generated using generalized boosted models was used to assess the risk of OAB among donepezil, galantamine, and rivastigmine users. RESULTS: The study included 524,975 older adults with dementia who were incident users of ChEIs (donepezil 80.72%, rivastigmine 16.41%, galantamine 2.87%). Overall, OAB diagnosis/antimuscarinic/mirabegron prescription was observed in 5.07% of the cohort within 6 months of ChEIs prescription. The Cox regression model with PS as covariate approach found that donepezil use increased the risk of OAB compared to rivastigmine (aHR, 1.13; 95% CI, 1.08-1.28; p < 0.0001). However, there was no differential risk of OAB between galantamine and rivastigmine. The findings were consistent with the generalized boosted models. CONCLUSIONS: The study found that the risk of OAB varies across individual ChEIs with an increased risk of OAB with donepezil compared to rivastigmine. The study findings suggest the need to understand and manage medication-related morbidity in older adults with dementia.
ESTHER : Masurkar_2021_J.Am.Geriatr.Soc__
PubMedSearch : Masurkar_2021_J.Am.Geriatr.Soc__
PubMedID: 34854475

Title : Discovery of small-molecule enzyme activators by activity-based protein profiling - Kok_2020_Nat.Chem.Biol_16_997
Author(s) : Kok BP , Ghimire S , Kim W , Chatterjee S , Johns T , Kitamura S , Eberhardt J , Ogasawara D , Xu J , Sukiasyan A , Kim SM , Godio C , Bittencourt JM , Cameron M , Galmozzi A , Forli S , Wolan DW , Cravatt BF , Boger DL , Saez E
Ref : Nat Chemical Biology , 16 :997 , 2020
Abstract : Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse-small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assay, we identify compounds that stimulate the activity of LYPLAL1-a poorly characterized serine hydrolase with complex genetic links to human metabolic traits. We apply ABPP-guided medicinal chemistry to advance a lead into a selective LYPLAL1 activator suitable for use in vivo. Structural simulations coupled to mutational, biochemical and biophysical analyses indicate that this compound increases LYPLAL1's catalytic activity likely by enhancing the efficiency of the catalytic triad charge-relay system. Treatment with this LYPLAL1 activator confers beneficial effects in a mouse model of diet-induced obesity. These findings reveal a new mode of pharmacological regulation for this large enzyme family and suggest that ABPP may aid discovery of activators for additional enzyme classes.
ESTHER : Kok_2020_Nat.Chem.Biol_16_997
PubMedSearch : Kok_2020_Nat.Chem.Biol_16_997
PubMedID: 32514184
Gene_locus related to this paper: human-LYPLAL1

Title : N-Acyl pyrazoles: Effective and tunable inhibitors of serine hydrolases - Otrubova_2019_Bioorg.Med.Chem_27_1693
Author(s) : Otrubova K , Chatterjee S , Ghimire S , Cravatt BF , Boger DL
Ref : Bioorganic & Medicinal Chemistry , 27 :1693 , 2019
Abstract : A series of N-acyl pyrazoles was examined as candidate serine hydrolase inhibitors in which the active site acylating reactivity and the leaving group ability of the pyrazole could be tuned not only through the nature of the acyl group (reactivity: amide > carbamate > urea), but also through pyrazole C4 substitution with electron-withdrawing or electron-donating substituents. Their impact on enzyme inhibitory activity displayed pronounced effects with the activity improving substantially as one alters both the nature of the reacting carbonyl group (urea > carbamate > amide) and the pyrazole C4 substituent (CN > H > Me). It was further demonstrated that the acyl chain of the N-acyl pyrazole ureas can be used to tailor the potency and selectivity of the inhibitor class to a targeted serine hydrolase. Thus, elaboration of the acyl chain of pyrazole-based ureas provided remarkably potent, irreversible inhibitors of fatty acid amide hydrolase (FAAH, apparent K(i) = 100-200 pM), dual inhibitors of FAAH and monoacylglycerol hydrolase (MGLL), or selective inhibitors of MGLL (IC(50) = 10-20 nM) while simultaneously minimizing off-target activity (e.g., ABHD6 and KIAA1363).
ESTHER : Otrubova_2019_Bioorg.Med.Chem_27_1693
PubMedSearch : Otrubova_2019_Bioorg.Med.Chem_27_1693
PubMedID: 30879861

Title : Biodegradation of di-n-butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway - Mahajan_2019_Int.J.Biol.Macromol_122_806
Author(s) : Mahajan R , Verma S , Kushwaha M , Singh D , Akhter Y , Chatterjee S
Ref : Int J Biol Macromol , 122 :806 , 2019
Abstract : A priority pollutant Phthalate Esters (PAEs) are widely used as plasticizers and are responsible mainly for carcinogenicity and endocrine disruption in human. For the bioremediation of PAEs, a psychrotolerant Sphingobium yanoikuyae strain P4, capable of utilizing many phthalates dimethyl phthalate (DMP), diethyl phthalate (DEP), dinbutyl phthalate (DBP), diisobutyl phthalate (DIBP), butyl benzyl phthalate (BBP), and few Polycyclic Aromatic Hydrocarbons as the sole source of carbon and energy was isolated from Palampur, Kangra, Himachal Pradesh, India. 100% utilization of DBP (1gL(-1)) by the strain was observed within 24h of incubation at 28 degrees C. Interestingly the strain also degraded DBP completely at 20 degrees C and 15 degrees C within 36h and 60h, respectively. Esterase involved in DBP degradation was found to be inducible in nature and intracellular. Comparative sequence analysis of carboxylesterase enzyme sequences revealed conserved motifs: G-X-S-X-G and -HGG- which were the characteristic peptide motifs reported in different esterases. Structural analysis showed that the enzyme belongs to serine hydrolase superfamily, which has an alpha/beta hydrolase fold. Interaction and binding of DBP to a catalytic Ser(184) residue in the esterase enzyme were also analysed. In conclusion, carboxylesterase possess the required active site which may be involved in the catabolism of DBP.
ESTHER : Mahajan_2019_Int.J.Biol.Macromol_122_806
PubMedSearch : Mahajan_2019_Int.J.Biol.Macromol_122_806
PubMedID: 30395899
Gene_locus related to this paper: sphya-k9da91

Title : Testing the Ret and Sema3d genetic interaction in mouse enteric nervous system development - Kapoor_2017_Hum.Mol.Genet_26_1811
Author(s) : Kapoor A , Auer DR , Lee D , Chatterjee S , Chakravarti A
Ref : Hum Mol Genet , 26 :1811 , 2017
Abstract : For most multigenic disorders, clinical manifestation (penetrance) and presentation (expressivity) are likely to be an outcome of genetic interaction between multiple susceptibility genes. Here, using gene knockouts in mice, we evaluated genetic interaction between loss of Ret and loss of Sema3d, two Hirschsprung disease susceptibility genes. We intercrossed Ret and Sema3d double null heterozygotes to generate mice with the nine possible genotypes and assessed survival by counting various genotypes, myenteric plexus presence by acetylcholinesterase staining and embryonic day 12.5 (E12.5) intestine transcriptome by RNA-sequencing. Survival rates of Ret wild-type, null heterozygote and null homozygote mice at E12.5, birth and weaning were not influenced by the genotypes at Sema3d locus and vice versa. Loss of myenteric plexus was observed only in all Ret null homozygotes, irrespective of the genotypes at Sema3d locus, and Sema3d null heterozygote and homozygote mice had normal intestinal innervation. As compared with wild-type mice intestinal gene expression, loss of Ret in null homozygotes led to differential expression of approximately 300 genes, whereas loss of Sema3d in null homozygotes had no major consequence and there was no evidence supporting major interaction between the two genes influencing intestine transcriptome. Overall, given the null alleles and phenotypic assays used, we did not find evidence for genetic interaction between Ret and Sema3d affecting survival, presence of myenteric plexus or intestine transcriptome.
ESTHER : Kapoor_2017_Hum.Mol.Genet_26_1811
PubMedSearch : Kapoor_2017_Hum.Mol.Genet_26_1811
PubMedID: 28334784

Title : Profenofos, an Acetylcholinesterase-Inhibiting Organophosphorus Pesticide: A Short Review of Its Usage, Toxicity, and Biodegradation - Kushwaha_2016_J.Environ.Qual_45_1478
Author(s) : Kushwaha M , Verma S , Chatterjee S
Ref : J Environ Qual , 45 :1478 , 2016
Abstract : Pesticides play an important role in the protection of different crops. Among the diverse sets of pesticides used all over the world, the organophosphates are the most widely used group. Profenofos [O-(4-bromo-2-chlorophenyl) O-ethyl S-propyl phosphorothioate] is one of the most largely used organophosphate insecticides on field crops, vegetables, and fruit crops. The World Health Organization classifies this compound as moderately hazardous (Toxicity Class II), and its residues have been found in vegetables like okra [ (L.) Moench], gooseberries ( sp.), green chilies [ (L.)], curry leaves [ (L.) Spreng], mint leaves [ (L.)], and coriander leaves [ (L.)]. Dietary intake of profenofos (PFF) is the major exposure pathway for humans. When applied to agricultural fields, PFF residues spread into every part of the environment: ambient air, surface water, and soil. In this review, we discuss the worldwide usage of PFF pesticide, its toxic effects on humans and other living organisms in the environment, and biodegradation of this chemical by various microbial strains. To date, no complete biodegradation pathway has been established for PFF pesticide, calling for a study of this nature.
ESTHER : Kushwaha_2016_J.Environ.Qual_45_1478
PubMedSearch : Kushwaha_2016_J.Environ.Qual_45_1478
PubMedID: 27695768

Title : Draft genome of a commonly misdiagnosed multidrug resistant pathogen Candida auris - Chatterjee_2015_BMC.Genomics_16_686
Author(s) : Chatterjee S , Alampalli SV , Nageshan RK , Chettiar ST , Joshi S , Tatu US
Ref : BMC Genomics , 16 :686 , 2015
Abstract : BACKGROUND: Candida auris is a multidrug resistant, emerging agent of fungemia in humans. Its actual global distribution remains obscure as the current commercial methods of clinical diagnosis misidentify it as C. haemulonii. Here we report the first draft genome of C. auris to explore the genomic basis of virulence and unique differences that could be employed for differential diagnosis. RESULTS: More than 99.5 % of the C. auris genomic reads did not align to the current whole (or draft) genome sequences of Candida albicans, Candida lusitaniae, Candida glabrata and Saccharomyces cerevisiae; thereby indicating its divergence from the active Candida clade. The genome spans around 12.49 Mb with 8527 predicted genes. Functional annotation revealed that among the sequenced Candida species, it is closest to the hemiascomycete species Clavispora lusitaniae. Comparison with the well-studied species Candida albicans showed that it shares significant virulence attributes with other pathogenic Candida species such as oligopeptide transporters, mannosyl transfersases, secreted proteases and genes involved in biofilm formation. We also identified a plethora of transporters belonging to the ABC and major facilitator superfamily along with known MDR transcription factors which explained its high tolerance to antifungal drugs. CONCLUSIONS: Our study emphasizes an urgent need for accurate fungal screening methods such as PCR and electrophoretic karyotyping to ensure proper management of fungemia. Our work highlights the potential genetic mechanisms involved in virulence and pathogenicity of an important emerging human pathogen namely C. auris. Owing to its diversity at the genomic scale; we expect the genome sequence to be a useful resource to map species specific differences that will help develop accurate diagnostic markers and better drug targets.
ESTHER : Chatterjee_2015_BMC.Genomics_16_686
PubMedSearch : Chatterjee_2015_BMC.Genomics_16_686
PubMedID: 26346253
Gene_locus related to this paper: canar-a0a2h1a5v8

Title : Partial agonists of the alpha3beta4* neuronal nicotinic acetylcholine receptor reduce ethanol consumption and seeking in rats - Chatterjee_2011_Neuropsychopharmacology_36_603
Author(s) : Chatterjee S , Steensland P , Simms JA , Holgate J , Coe JW , Hurst RS , Shaffer CL , Lowe J , Rollema H , Bartlett SE
Ref : Neuropsychopharmacology , 36 :603 , 2011
Abstract : Alcohol use disorders (AUDs) impact millions of individuals and there remain few effective treatment strategies. Despite evidence that neuronal nicotinic acetylcholine receptors (nAChRs) have a role in AUDs, it has not been established which subtypes of the nAChR are involved. Recent human genetic association studies have implicated the gene cluster CHRNA3-CHRNA5-CHRNB4 encoding the alpha3, alpha5, and beta4 subunits of the nAChR in susceptibility to develop nicotine and alcohol dependence; however, their role in ethanol-mediated behaviors is unknown due to the lack of suitable and selective research tools. To determine the role of the alpha3, and beta4 subunits of the nAChR in ethanol self-administration, we developed and characterized high-affinity partial agonists at alpha3beta4 nAChRs, CP-601932, and PF-4575180. Both CP-601932 and PF-4575180 selectively decrease ethanol but not sucrose consumption and operant self-administration following long-term exposure. We show that the functional potencies of CP-601932 and PF-4575180 at alpha3beta4 nAChRs correlate with their unbound rat brain concentrations, suggesting that the effects on ethanol self-administration are mediated via interaction with alpha3beta4 nAChRs. Also varenicline, an approved smoking cessation aid previously shown to decrease ethanol consumption and seeking in rats and mice, reduces ethanol intake at unbound brain concentrations that allow functional interactions with alpha3beta4 nAChRs. Furthermore, the selective alpha4beta2(*) nAChR antagonist, DHbetaE, did not reduce ethanol intake. Together, these data provide further support for the human genetic association studies, implicating CHRNA3 and CHRNB4 genes in ethanol-mediated behaviors. CP-601932 has been shown to be safe in humans and may represent a potential novel treatment for AUDs.
ESTHER : Chatterjee_2011_Neuropsychopharmacology_36_603
PubMedSearch : Chatterjee_2011_Neuropsychopharmacology_36_603
PubMedID: 21048701

Title : Prevalence and predictors of anticholinergic agents in elderly outpatients with dementia - Bhattacharya_2011_Am.J.Geriatr.Pharmacother_9_434
Author(s) : Bhattacharya R , Chatterjee S , Carnahan RM , Aparasu RR
Ref : Am J Geriatr Pharmacother , 9 :434 , 2011
Abstract : BACKGROUND: Anticholinergic medications, although frequently used in elderly populations, are associated with cognitive impairment and constitute significant concern for patients with dementia. OBJECTIVE: The purpose of our study was to examine patterns and predictors of prescribing anticholinergic agents for elderly outpatients with dementia. METHODS: We combined data from the 2006-2007 National Ambulatory Medical Care Survey and the outpatient department component of National Hospital Ambulatory Medical Care Survey to analyze patient visits by elderly persons (aged >/=65 years) with dementia. Anticholinergic drugs were identified using the Anticholinergic Drug Scale, which classifies anticholinergic drugs into four levels (0-3) in increasing order of anticholinergic activity. Descriptive analysis using sampling weights was used to evaluate patterns of anticholinergic drug prescription, especially prescribing of medications with clinically significant anticholinergic activity (ie, levels 2 or 3). Multiple logistic regression was used in the conceptual framework of the Andersen Behavioral Model to examine the predisposing, enabling, and need factors associated with prescribing of medications with clinically significant anticholinergic activity. RESULTS: According to the national surveys there were a total of 6.8 million (95% CI, 5.27-8.44 million; 0.32%) ambulatory care visits for dementia. Approximately 43% (42.86%; 95% CI, 35.24-50.48) of these visits involved prescribing at least one anticholinergic drug; and 10.07% of visits involved prescribing levels 2 or 3 anticholinergic medications. The predisposing factor, age (75-84 years; odds ratio [OR] = 0.25; 95% CI, 0.07-0.87), and the need factors, acetylcholinesterase inhibitor use (OR = 0.25; 95% CI, 0.07-0.86) and comorbid mood disorders (OR = 0.12; 95% CI, 0.02-0.73), were associated with decreased likelihood of prescribing medications with clinically significant anticholinergic activity. The need factor total number of medications prescribed (OR = 1.45, 95% CI, 1.20-1.75) increased the likelihood of these prescriptions being administered. CONCLUSIONS: One in 10 outpatient visits by elderly persons with dementia involved prescribing medications with clinically significant anticholinergic activity. Given their adverse cognitive effects, there is a strong need to optimize anticholinergic drug prescribing in vulnerable elderly outpatients with dementia.
ESTHER : Bhattacharya_2011_Am.J.Geriatr.Pharmacother_9_434
PubMedSearch : Bhattacharya_2011_Am.J.Geriatr.Pharmacother_9_434
PubMedID: 22030114

Title : Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos - Viswanath_2010_J.Steroid.Biochem.Mol.Biol_120_22
Author(s) : Viswanath G , Chatterjee S , Dabral S , Nanguneri SR , Divya G , Roy P
Ref : J Steroid Biochem Mol Biol , 120 :22 , 2010
Abstract : The present work describes the screening and characterization of some common endocrine disrupting chemicals for their (anti)androgenic activities. Various chemicals (mostly pesticides and pharmaceuticals) were screened with the NIH3T3 cell line stably expressing human androgen receptor (hAR) and luciferase reporter gene for their ability to stimulate luciferase activity or inhibit the response that was evoked by 0.4nM testosterone. The most potent anti-androgenic compounds identified in our assay included chlorpyrifos, endosulfan and piperophos. Finally, the chemicals were analyzed for their effects on steriodogenesis in rat Leydig cells. Piperophos and chlorpyrifos showed a significant decrease in testosterone biosynthesis by Leydig cells. RT-PCR studies showed decrease in the expression of key steroidogenic enzymes: cytochrome P450scc, 3beta-HSD and 17beta-HSD and immunoblot analysis demonstrated a decrease in steroidogenic acute regulatory (StAR) protein expression by both these chemicals. Chlorpyrifos also showed a decrease in LH receptor stimulated cAMP production. In conclusion, we demonstrate that commonly used pesticides like chlorpyrifos and piperophos pose serious threat to male reproductive system by interfering at various levels of androgen biosynthesis.
ESTHER : Viswanath_2010_J.Steroid.Biochem.Mol.Biol_120_22
PubMedSearch : Viswanath_2010_J.Steroid.Biochem.Mol.Biol_120_22
PubMedID: 20211256

Title : Silk-fiber immobilized lipase-catalyzed hydrolysis of emulsified sunflower oil - Chatterjee_2009_Appl.Biochem.Biotechnol_157_593
Author(s) : Chatterjee S , Barbora L , Cameotra SS , Mahanta P , Goswami P
Ref : Appl Biochem Biotechnol , 157 :593 , 2009
Abstract : Lipase was immobilized in silk fibers through glutaraldehyde cross-linking to a maximum loading of 59 U/g silk-fiber and the immobilized lipase was utilized for the hydrolysis of sunflower oil (Helianthus annuus). The hydrolytic activity of the lipase, which was poor in biphasic oil in water system, was increased significantly when the sunflower oil was emulsified in aqueous medium. The hydrolytic activities of the immobilized lipase were 48.73 +/- 1.26 U, 36.11 +/- 0.96 U, and nil when the substrate sunflower oil was used as emulsion created by a rhamnolipid biosurfactant, Triton X100, and ultrasonication, respectively. Although the efficiency of the immobilized lipase was less than 12% than the corresponding free lipase, the immobilized lipase could be reused for the biosurfactant-mediated hydrolysis of sunflower oil up to third cycle of the reaction. The yield of the fatty acids in the second, third, and fourth cycles were 49.45%, 22.91%, and 5.09%, respectively, of the yield obtained in the first cycle.
ESTHER : Chatterjee_2009_Appl.Biochem.Biotechnol_157_593
PubMedSearch : Chatterjee_2009_Appl.Biochem.Biotechnol_157_593
PubMedID: 19002611

Title : Metabolic cooperation of Gordonia sp. strain MTCC 4818 and Arthrobacter sp. strain WY in the utilization of butyl benzyl phthalate: effect of a novel co-culture in the degradation of a mixture of phthalates - Chatterjee_2008_Microbiol.(Reading)_154_3338
Author(s) : Chatterjee S , Dutta TK
Ref : Microbiology (Reading) , 154 :3338 , 2008
Abstract : Degradation of butyl benzyl phthalate (BBP) by a co-culture of Gordonia sp. strain MTCC 4818 and Arthrobacter sp. strain WY was investigated. In the degradation of BBP by the co-culture, the limitations of the individual species in metabolizing BBP were overcome, leading to the development of a consortium capable of complete utilization of this ester. In the degradation of BBP by the co-culture, the presence of multiple esterases was demonstrated in both species by activity staining of non-denaturing gels, indicating their roles in the degradation process. The esterases were found to be inducible, with unique or broad substrate specificities towards BBP and its monoesters. Moreover, a number of catabolic enzymes other than esterases identified in the metabolism of BBP-degraded intermediates facilitated the co-culture-mediated degradation process. The versatility of the co-culture was further established by the rapid and complete degradation of a mixture of phthalate esters of environmental concern.
ESTHER : Chatterjee_2008_Microbiol.(Reading)_154_3338
PubMedSearch : Chatterjee_2008_Microbiol.(Reading)_154_3338
PubMedID: 18957587

Title : Disruption of pituitary-ovarian axis by carbofuran in catfish, Heteropneustes fossilis (Bloch) - Chatterjee_2001_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_129_265
Author(s) : Chatterjee S , Kumar Dasmahapatra A , Ghosh R
Ref : Comparative Biochemistry & Physiology C Pharmacol Toxicol , 129 :265 , 2001
Abstract : We investigated whether carbofuran (CF), a carbamate pesticide, at sub-lethal concentration had any adverse effects on reproductive function of the Indian catfish, Heteropneustes fossilis. 17beta-Estradiol content of serum and ovary of pre-spawning (P) and spawning (S) fish was reduced after sub-lethal concentration of carbofuran treatment (0.5-2 mg/ml, 30 days). After 30 days of CF treatment, the serum and ovarian vitellogenin levels of fish at the P stage were also reduced but remained unaltered in the S stage. The staining intensity of the pituitary gonadotrophs of the pre-spawning fish was significantly higher in CF-treated fish compared to controls suggesting the inability of the pituitary gonadotrophs to release gonadotropin following CF treatment. CF thus acts as an antiestrogenic, endocrine-disrupting agent in fish, possibly targeting the pituitary-gonad axis.
ESTHER : Chatterjee_2001_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_129_265
PubMedSearch : Chatterjee_2001_Comp.Biochem.Physiol.C.Toxicol.Pharmacol_129_265
PubMedID: 11461841

Title : Impact of carbofuran in the oocyte maturation of catfish, Heteropenustes fossilis (Bloch) - Chatterjee_1997_Arch.Environ.Contam.Toxicol_32_426
Author(s) : Chatterjee S , Dutta AB , Ghosh R
Ref : Archives of Environmental Contamination & Toxicology , 32 :426 , 1997
Abstract : Investigations were undertaken to observe the influence of technical grade carbofuran (CF) on the egg maturational processes of catfish, Heteropneustes fossilis (Bloch). Fish were exposed to sublethal doses (0.5, 1, and 2 mg/L) of CF for 30 days at 25 +/- 1 degrees C. Control fish were maintained in water. The gonado-somatic index (G.S. I. = ovarian weight x 100/body weight) of the fish, treated with 1 or 2 mg/L CF, was decreased significantly compared to that of the controls. The inhibition was almost at the same level in these doses. CF at the dose of 0.5 mg/L was found to be ineffective. From the histomorphological observations of the ovary, it was observed that CF altered both the area and the percentage occurrence of the various types of primary oocytes in the ovary compared to that of the control fish. The stage I primary oocytes were predominantly higher in CF-treated fish than stage II and stage III which was reversed in control animals. The degeneration of follicular walls, connective tissues and vacuolization in the ooplasm of the stage II and III oocytes were observed in CF-treated fish (0.5-2 mg/L). It appears that CF at sublethal concentrations inhibits oocyte maturational processes in catfish.
ESTHER : Chatterjee_1997_Arch.Environ.Contam.Toxicol_32_426
PubMedSearch : Chatterjee_1997_Arch.Environ.Contam.Toxicol_32_426
PubMedID: 9175511

Title : Evaluation of urinary cells in acid cholesteryl ester hydrolase deficiency - Chatterjee_1986_Clin.Genet_29_360
Author(s) : Chatterjee S , Castiglione E , Kwiterovich PO, Jr. , Hoeg JM , Brewer HB
Ref : Clin Genet , 29 :360 , 1986
Abstract : Deficiency in the lysosomal enzyme responsible for cholesteryl ester hydrolysis, acid cholesteryl ester hydrolase (E.C., leads to two clinically recognized diseases: Wolman disease and cholesteryl ester storage including leukocytes, fibroblasts and liver. Analysis of urinary sediment from well characterized cases of Wolman disease and CESD also revealed the shedding of lipid enriched renal tubular cells. Morphologic, enzymic and lipid compositional studies of these cells indicate that the enzyme deficiency observed in fibroblasts and leukocytes from these individuals are reflected in these cells shed in the urine. These findings in renal tubular cells confirm and extend those made in other cell types. These studies indicate that analysis of urinary sediment in suspected cases of acid cholesteryl ester deficiency may provide a meaningful approach for monitoring therapeutic attempts involving enzyme infusion and gene therapy.
ESTHER : Chatterjee_1986_Clin.Genet_29_360
PubMedSearch : Chatterjee_1986_Clin.Genet_29_360
PubMedID: 3742843