Mahajan_2019_Int.J.Biol.Macromol_122_806

Reference

Title : Biodegradation of di-n-butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway - Mahajan_2019_Int.J.Biol.Macromol_122_806
Author(s) : Mahajan R , Verma S , Kushwaha M , Singh D , Akhter Y , Chatterjee S
Ref : Int J Biol Macromol , 122 :806 , 2019
Abstract :

A priority pollutant Phthalate Esters (PAEs) are widely used as plasticizers and are responsible mainly for carcinogenicity and endocrine disruption in human. For the bioremediation of PAEs, a psychrotolerant Sphingobium yanoikuyae strain P4, capable of utilizing many phthalates dimethyl phthalate (DMP), diethyl phthalate (DEP), dinbutyl phthalate (DBP), diisobutyl phthalate (DIBP), butyl benzyl phthalate (BBP), and few Polycyclic Aromatic Hydrocarbons as the sole source of carbon and energy was isolated from Palampur, Kangra, Himachal Pradesh, India. 100% utilization of DBP (1gL(-1)) by the strain was observed within 24h of incubation at 28 degrees C. Interestingly the strain also degraded DBP completely at 20 degrees C and 15 degrees C within 36h and 60h, respectively. Esterase involved in DBP degradation was found to be inducible in nature and intracellular. Comparative sequence analysis of carboxylesterase enzyme sequences revealed conserved motifs: G-X-S-X-G and -HGG- which were the characteristic peptide motifs reported in different esterases. Structural analysis showed that the enzyme belongs to serine hydrolase superfamily, which has an alpha/beta hydrolase fold. Interaction and binding of DBP to a catalytic Ser(184) residue in the esterase enzyme were also analysed. In conclusion, carboxylesterase possess the required active site which may be involved in the catabolism of DBP.

PubMedSearch : Mahajan_2019_Int.J.Biol.Macromol_122_806
PubMedID: 30395899
Gene_locus related to this paper: sphya-k9da91

Related information

Substrate Dibutyl-phthalate
Gene_locus Dibutyl-phthalate    sphya-k9da91

Citations formats

Mahajan R, Verma S, Kushwaha M, Singh D, Akhter Y, Chatterjee S (2019)
Biodegradation of di-n-butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway
Int J Biol Macromol 122 :806

Mahajan R, Verma S, Kushwaha M, Singh D, Akhter Y, Chatterjee S (2019)
Int J Biol Macromol 122 :806