Leshkowitz D

References (3)

Title : Dicer Deficiency Differentially Impacts Microglia of the Developing and Adult Brain - Varol_2017_Immunity_46_1030
Author(s) : Varol D , Mildner A , Blank T , Shemer A , Barashi N , Yona S , David E , Boura-Halfon S , Segal-Hayoun Y , Chappell-Maor L , Keren-Shaul H , Leshkowitz D , Hornstein E , Fuhrmann M , Amit I , Maggio N , Prinz M , Jung S
Ref : Immunity , 46 :1030 , 2017
Abstract : Microglia seed the embryonic neuro-epithelium, expand and actively sculpt neuronal circuits in the developing central nervous system, but eventually adopt relative quiescence and ramified morphology in the adult. Here, we probed the impact of post-transcriptional control by microRNAs (miRNAs) on microglial performance during development and adulthood by generating mice lacking microglial Dicer expression at these distinct stages. Conditional Dicer ablation in adult microglia revealed that miRNAs were required to limit microglial responses to challenge. After peripheral endotoxin exposure, Dicer-deficient microglia expressed more pro-inflammatory cytokines than wild-type microglia and thereby compromised hippocampal neuronal functions. In contrast, prenatal Dicer ablation resulted in spontaneous microglia activation and revealed a role for Dicer in DNA repair and preservation of genome integrity. Accordingly, Dicer deficiency rendered otherwise radio-resistant microglia sensitive to gamma irradiation. Collectively, the differential impact of the Dicer ablation on microglia of the developing and adult brain highlights the changes these cells undergo with time.
ESTHER : Varol_2017_Immunity_46_1030
PubMedSearch : Varol_2017_Immunity_46_1030
PubMedID: 28636953

Title : Genome sequence of the pattern-forming social bacterium Paenibacillus dendritiformis C454 chiral morphotype - Sirota-Madi_2012_J.Bacteriol_194_2127
Author(s) : Sirota-Madi A , Olender T , Helman Y , Brainis I , Finkelshtein A , Roth D , Hagai E , Leshkowitz D , Brodsky L , Galatenko V , Nikolaev V , Gutnick DL , Lancet D , Ben-Jacob E
Ref : Journal of Bacteriology , 194 :2127 , 2012
Abstract : Paenibacillus dendritiformis is a Gram-positive, soil-dwelling, spore-forming social microorganism. An intriguing collective faculty of this strain is manifested by its ability to switch between different morphotypes, such as the branching (T) and the chiral (C) morphotypes. Here we report the 6.3-Mb draft genome sequence of the P. dendritiformis C454 chiral morphotype.
ESTHER : Sirota-Madi_2012_J.Bacteriol_194_2127
PubMedSearch : Sirota-Madi_2012_J.Bacteriol_194_2127
PubMedID: 22461558
Gene_locus related to this paper: 9bacl-h3sdn5 , 9bacl-h3sft0 , 9bacl-h3sg17 , 9bacl-h3smu8

Title : Genome sequence of the pattern forming Paenibacillus vortex bacterium reveals potential for thriving in complex environments - Sirota-Madi_2010_BMC.Genomics_11_710
Author(s) : Sirota-Madi A , Olender T , Helman Y , Ingham C , Brainis I , Roth D , Hagi E , Brodsky L , Leshkowitz D , Galatenko V , Nikolaev V , Mugasimangalam RC , Bransburg-Zabary S , Gutnick DL , Lancet D , Ben-Jacob E
Ref : BMC Genomics , 11 :710 , 2010
Abstract : BACKGROUND: The pattern-forming bacterium Paenibacillus vortex is notable for its advanced social behavior, which is reflected in development of colonies with highly intricate architectures. Prior to this study, only two other Paenibacillus species (Paenibacillus sp. JDR-2 and Paenibacillus larvae) have been sequenced. However, no genomic data is available on the Paenibacillus species with pattern-forming and complex social motility. Here we report the de novo genome sequence of this Gram-positive, soil-dwelling, sporulating bacterium.
RESULTS: The complete P. vortex genome was sequenced by a hybrid approach using 454 Life Sciences and Illumina, achieving a total of 289x coverage, with 99.8% sequence identity between the two methods. The sequencing results were validated using a custom designed Agilent microarray expression chip which represented the coding and the non-coding regions. Analysis of the P. vortex genome revealed 6,437 open reading frames (ORFs) and 73 non-coding RNA genes. Comparative genomic analysis with 500 complete bacterial genomes revealed exceptionally high number of two-component system (TCS) genes, transcription factors (TFs), transport and defense related genes. Additionally, we have identified genes involved in the production of antimicrobial compounds and extracellular degrading enzymes.
CONCLUSIONS: These findings suggest that P. vortex has advanced faculties to perceive and react to a wide range of signaling molecules and environmental conditions, which could be associated with its ability to reconfigure and replicate complex colony architectures. Additionally, P. vortex is likely to serve as a rich source of genes important for agricultural, medical and industrial applications and it has the potential to advance the study of social microbiology within Gram-positive bacteria.
ESTHER : Sirota-Madi_2010_BMC.Genomics_11_710
PubMedSearch : Sirota-Madi_2010_BMC.Genomics_11_710
PubMedID: 21167037
Gene_locus related to this paper: 9bacl-e5yrc1 , 9bacl-e5yu37