Liu ZM

References (9)

Title : Improving the Efficiency of Precise Genome Editing with CRISPR\/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase - Wang_2023_Cells_12_
Author(s) : Wang JH , Wu SJ , Li Y , Zhao Y , Liu ZM , Deng SL , Lian ZX
Ref : Cells , 12 : , 2023
Abstract : The CRISPR/Cas9 system is widely used for genome editing in livestock production, although off-target effects can occur. It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs). Improving the double-strand break (DSB) efficiency of Cas9 in primary cells would improve the homologous repair (HR) efficiency. The low efficiency of HR remains a major hurdle in CRISPR/Cas9-mediated precise genome editing, increasing the work required to screen the genome-edited primary cell clones. In this study, we modified several essential parameters that affect the efficiency of the CRISPR/Cas9-mediated knock-in GFF cloning system, including establishing a high-efficiency transfection system for primary cells via nucleofection and optimizing homology arm (HA) length during HR. Here, we specifically inserted a recombinant human butyrylcholinesterase gene (rhBChE) into the goat fibroblast growth factor (FGF)-5 locus through the CRISPR/Cas9 system, thereby achieving simultaneous rhBChE insertion and FGF5 knock-out. First, this study introduced the Cas9, FGF5 knock-out small guide RNA, and rhBChE knock-in donors into GFFs by electroporation and obtained positive cell clones without off-target effects. Then, we demonstrated the expression of rhBChE in GFF clones and verified its function. Finally, we obtained a CRISPR/Cas9-mediated rhBChE-overexpression goat.
ESTHER : Wang_2023_Cells_12_
PubMedSearch : Wang_2023_Cells_12_
PubMedID: 37508483

Title : [Clinical and genetic characteristics of congenital myasthenia syndrome with episodic apnea caused by CHAT gene mutation: a report of 2 cases] - Liu_2018_Zhonghua.Er.Ke.Za.Zhi_56_216
Author(s) : Liu ZM , Fang F , Ding CH , Zhang WH , Deng J , Chen CH , Wang X , Liu J , Li Z , Jia XL , Zeng JS , Qian SY
Ref : Zhonghua Er Ke Za Zhi , 56 :216 , 2018
Abstract : Objective: To investigate the clinical and genetic features of congenital myasthenia syndrome with episodic apnea (CMS-EA) caused by gene mutation of choline acetyltransferase (CHAT) Methods: The clinical data of 2 patients with congenital myasthenia syndrome were collected, and both were diagnosed from 2013 to 2015 in Beijing Children's Hospital, Capital Medical University. The clinical features and gene mutation characteristics were analyzed, and the patients were followed-up for therapeutic efficacy. Results: The two patients (case 1 and case 2) had the onset soon after birth and at 3 months after birth respectively. The two patients were admitted to the PICU due to dyspnea, cyanotic episodes that required intubation. The patients had repeated apnea and became ventilator dependent. Case 1 died due to refusal of any treatment. Case 2 had a tracheotomy, and gradually weaned from ventilator after using pyridostigmine. The hospitalization of case 2 lasted 162 days. Case 2 was followed up to the age of 3 years and 4 months, and was extubated and was maintained on oral neostigmine but still had fluctuating ptosis and minor physical and mental retardation. Both cases were negative for anti-AChR, anti-acetylcholinesterase, anti-MuSK antibodies. Neostigmine test was negative in case 1 and suspiciously positive in case 2. Low-frequency repetitive nerve stimulation testing of case 2 was negative. Cranial MRI scans of both cases showed brain atrophy-like change. Genetic testing showed compound heterozygous deletions (exon 4, 5, 6) and pathogenic variant c.914T>C (p.I305T) in CHAT in case 1, compound heterozygous variants c.1007T>C (p.I336T) and c.64C>T (p.Q22X) in CHAT in case 2. To our knowledge, compound heterozygous deletions (exon 4, 5, 6) and p.Q22X were novel, previously unreported variants. Conclusion: CMS-EA usually presents at birth or in the neonatal period with hypotonia, ptosis, dysphagia due to severe bulbar weakness, and respiratory insufficiency with cyanosis and apnea. Early treatment with pyridostigmine is helpful to the improvement of clinical symptoms and prognosis.
ESTHER : Liu_2018_Zhonghua.Er.Ke.Za.Zhi_56_216
PubMedSearch : Liu_2018_Zhonghua.Er.Ke.Za.Zhi_56_216
PubMedID: 29518833

Title : Molecular interaction studies of acetylcholinesterase with potential acetylcholinesterase inhibitors from the root of Rhodiola crenulata using molecular docking and isothermal titration calorimetry methods - Li_2017_Int.J.Biol.Macromol_104_527
Author(s) : Li FJ , Liu Y , Yuan Y , Yang B , Liu ZM , Huang LQ
Ref : Int J Biol Macromol , 104 :527 , 2017
Abstract : (-)-Epicatechin gallate ((-)-ECG), 1,2,3,4,6-O-pentagalloylglucose (PGG), rhodionin, herbacetin and rhodiosin isolated from the root of Rhodiola crenulata exhibited potent, dose-dependent inhibitory effects on acetylcholinesterase (AChE) with IC50 ranged from 57.50+/-5.83 to 2.43+/-0.34mug/mL. With the aim of explaining the differences in activity of these active ingredients and clarifying how they inhibit AChE, the AChE-inhibitor interactions were further explored using molecular docking and isothermal titration calorimetry (ITC) methods in the present study. Molecular docking studies revealed that all compounds except PGG showed binding energy values ranging from -10.30 to -8.00kcal/mol while the binding energy of galantamine, a known AChE inhibitor, was -9.53kcal/mol; they inhibited the AChE by binding into the ligand pocket with the similar binding pattern to that of galantamine by interacting with Glu199 of AChE. Inhibition constant of these active ingredients had a positive correlation with binding energy. The interaction between AChE and PGG was further evaluated with the ITC method and the results indicated that the PGG-AChE interaction was relevant to AChE concentration. The results revealed a possible mechanism for the AChE inhibition activity of these bioactive ingredients, which may provide some help in lead compounds optimization in the future.
ESTHER : Li_2017_Int.J.Biol.Macromol_104_527
PubMedSearch : Li_2017_Int.J.Biol.Macromol_104_527
PubMedID: 28625836

Title : Fructus Psoraleae contains natural compounds with potent inhibitory effects towards human carboxylesterase 2 - Li_2015_Fitoterapia_101_99
Author(s) : Li YG , Hou J , Li SY , Lv X , Ning J , Wang P , Liu ZM , Ge GB , Ren JY , Yang L
Ref : Fitoterapia , 101 :99 , 2015
Abstract : Fructus Psoraleae (FP) is an edible Chinese herbal which is widely used in Asia for the treatment of various diseases including asthma, diarrhea, and osteoporosis. This study aimed to investigate the inhibitory effects of the crude ethanol extract from FP on human carboxylesterase 2 (hCE2), as well as to identity and characterize the naturally occurring inhibitors of hCE2 in FP. Our results demonstrated that the ethanol extract of FP displayed potent inhibitory effects towards hCE2, while five major bioactive constitutes in FP were efficiently identified by LC-DAD-ESI-MS/MS, with the aid of LC-based activity profiling. The identified bioactive compounds including neobavaisoflavone, isobavachalcone, bavachinin, corylifol A and bakuchiol were found to be naturally occurring potent inhibitors of hCE2, with low Ki values ranging from 0.62muM to 3.89muM. This is the first report of the chemical constitutes in FP as potent inhibitors of hCE2.
ESTHER : Li_2015_Fitoterapia_101_99
PubMedSearch : Li_2015_Fitoterapia_101_99
PubMedID: 25596095

Title : A highly selective ratiometric fluorescent probe for in vitro monitoring and cellular imaging of human carboxylesterase 1 - Liu_2014_Biosens.Bioelectron_57C_30
Author(s) : Liu ZM , Feng L , Ge GB , Lv X , Hou J , Cao YF , Cui JN , Yang L
Ref : Biosensors & Bioelectronics , 57C :30 , 2014
Abstract : A new ratiometric fluorescent probe derived from 2-(2-hydroxy-3-methoxyphenyl) benzothiazole (HMBT) has been developed for selective monitoring of human carboxylesterase 1 (hCE1). The probe is designed by introducing benzoyl moiety to HMBT. The prepared latent spectroscopic probe 1 displays satisfying stability under physiological pH conditions with very low background signal. Both the reaction phynotyping and chemical inhibition assays demonstrated that hCE1 mediated the specific cleavage of the carboxylic ester bond of probe 1 in human biological samples. The release of HMBT leads to a remarkable red-shifted emission in fluorescence spectrum (120nm large emission shift). Furthermore, human cell-based assays show that probe 1 is cell membrane permeable, and it can be used for bioassay and cellular imaging of hCE1 activity in HepG2 cells. These findings lead to the development of a simple and sensitive fluorescent method for measurement of hCE1 activity in vitro or in living cells, in the presence of additional enzymes or endogenous compounds.
ESTHER : Liu_2014_Biosens.Bioelectron_57C_30
PubMedSearch : Liu_2014_Biosens.Bioelectron_57C_30
PubMedID: 24534577

Title : A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells, - Liu_2014_Sens.Actuators.B.Chem_205_151
Author(s) : Liu ZM , Feng L , Hou J , Lv X , Ning J , Ge GB , Wang KW , Cui JN , Yang L
Ref : Sensors and Actuators B: Chemical , 205 :151 , 2014
Abstract : A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated.
ESTHER : Liu_2014_Sens.Actuators.B.Chem_205_151
PubMedSearch : Liu_2014_Sens.Actuators.B.Chem_205_151
PubMedID:

Title : A highly selective long-wavelength fluorescent probe for the detection of human carboxylesterase 2 and its biomedical applications - Feng_2014_Chem.Commun.(Camb)_50_14519
Author(s) : Feng L , Liu ZM , Xu L , Lv X , Ning J , Hou J , Ge GB , Cui JN , Yang L
Ref : Chem Commun (Camb) , 50 :14519 , 2014
Abstract : A highly selective long-wavelength fluorescent probe TCFB has been developed for the detection of hCE2. The probe can be used for real-time monitoring of hCE2 activity in complex biological systems.
ESTHER : Feng_2014_Chem.Commun.(Camb)_50_14519
PubMedSearch : Feng_2014_Chem.Commun.(Camb)_50_14519
PubMedID: 25303144
Gene_locus related to this paper: human-CES2

Title : A highly selective fluorescent ESIPT probe for the detection of Human carboxylesterase 2 and its biological applications - Feng_2014_Biosens.Bioelectron_65C_9
Author(s) : Feng L , Liu ZM , Hou J , Lv X , Ning J , Ge GB , Cui JN , Yang L
Ref : Biosensors & Bioelectronics , 65C :9 , 2014
Abstract : A new ratiometric florescence probe derived from 3-hydroxyflavone (3-HF) has been developed for selective and sensitive detection of human carboxylesterase 2 (CE2). The probe is designed by modulating the excited state intramolecular proton transfer (ESIPT) emission of 3-HF via introducing of 4-ethylbenzoyloxy group. Under physiological conditions, probe 1 displays satisfying stability with very low background signal, but it can be selectively hydrolyzed by CE2 to release free 3-HF which brings remarkable changes in fluorescence spectrum. Both reaction phenotyping and chemical inhibition assays demonstrate that probe 1 is highly selective for CE2 over other human hydrolases including carboxylesterase 1, cholinesterases and paraoxonases. Probe 1 has been applied successfully to measure the real activities of CE2 in human biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. Additionally, probe 1 is cell membrane permeable and can be used for cellular imaging of endogenous CE2 in living cells. All of these features make it possible to serve as a promising tool for exploring the individual differences in biological function of CE2, as well as for rapid screening of selective and potent inhibitors of CE2 for further clinical use.
ESTHER : Feng_2014_Biosens.Bioelectron_65C_9
PubMedSearch : Feng_2014_Biosens.Bioelectron_65C_9
PubMedID: 25461132

Title : [Production of functional lipids by lipase-catalyzed acidolysis of lard in solvent free system] - Zhao_2005_Sheng.Wu.Gong.Cheng.Xue.Bao_21_493
Author(s) : Zhao HZ , Lu ZX , Bie XM , Lu FX , Liu ZM
Ref : Sheng Wu Gong Cheng Xue Bao , 21 :493 , 2005
Abstract : China has richly and inexpensive fat and oils from animal and plants, but these resources could not get effectively utilization. In order to make the best of these resources, lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipid in solvent free system was investigated. Of the five lipases that were tested in the initial screening, immobilized lipase TL IM fromca T. languginosa resulted in the highest incorporation of capry lic acid into lard. This enzyme was further studied for the effect of enzyme load, substrate ratib, reaction time, reaction temperature and added water content on the incorporation of caprylic acid into lard. HPLC analyzed the products from the acidolysis reaction. The highest incorporation was attained at 20% enzyme load. Time course studied suggest that the incorporation of caprylic acid into lard was increased up to 38.77 mol% after 24h. Desirable mole ratio of substrates was 1:2 (lard: caprylic acid), caprylic acid incorporation up to 30.95 mol%. In the range of 45 - 60 degrees C , temperature had no significant effect on enzyme activity and caprylic acid incorporation changed little. When temperature was above 60 degrees C, incorporation of caprylic acid into lard was decreased. The highest incorporation of caprylic acid into lard 35.76 mol% was attained when added water content was 2.5%.
ESTHER : Zhao_2005_Sheng.Wu.Gong.Cheng.Xue.Bao_21_493
PubMedSearch : Zhao_2005_Sheng.Wu.Gong.Cheng.Xue.Bao_21_493
PubMedID: 16108382