Lv X

References (23)

Title : Construction of molecular enrichment accelerators via assembly of enzyme surface grafted polymer and cyclodextrin achieving rapid and stable ester catalysis for biodiesel synthesis - Ao_2023_Carbohydr.Polym_322_121337
Author(s) : Ao Q , Jiang L , Tong X , Song Y , Lv X , Tang J
Ref : Carbohydr Polym , 322 :121337 , 2023
Abstract : Efficient and stable catalysis has always been the core concept of enzyme catalysis in industrial processes for manufacturing. Here, we constructed molecular enrichment accelerators to synergistically enhance enzyme activity and stability by assembling enzyme surface grafted polymer and cyclodextrin. At 40 degreesC, the enzyme activity of CalB-PNIPAM(212)/beta-CD was 2.9 times that of CalB-PNIPAM(212). The enzyme activity of CalB-PNIPAM(428)/gamma-CD had reached 1.61 times that of CalB. At the same time, the stability of CalB-PNIPAM(212)/beta-CD and CalB-PNIPAM(428)/gamma-CD are slightly better than that of CalB under high temperature, organic solution and extreme pH conditions. The synergistic increase in activity and stability of the lipase-polymer assembly was achieved due to the structure of assembly, in which the role of cyclodextrin could enrich substrate affecting molecular diffusion. In addition, the lipase-polymer assembly proved to be an efficient catalyst for biodiesel synthesis, with a biodiesel conversion 1.4 times that of CalB at 60 degreesC. Therefore, this simple and low-cost lipase-polymer assembly provides new possibilities for the construction of high-efficiency industrial biocatalytic catalysts.
ESTHER : Ao_2023_Carbohydr.Polym_322_121337
PubMedSearch : Ao_2023_Carbohydr.Polym_322_121337
PubMedID: 37839844

Title : Unprecedented diterpenoid dimers with soluble epoxide hydrolase inhibitory effect from Euphorbia fischeriana - Zhao_2022_Org.Biomol.Chem__
Author(s) : Zhao WY , Sun CP , Chang YB , Wang WY , Yan JK , Lv X , Wang C , Ma XC
Ref : Org Biomol Chem , : , 2022
Abstract : Biseuphoids A (1) and B (2), two unprecedented ent-abietane-type diterpenoid dimers linked by monomeric blocks through C-17-C-12' and C-17-C-11', respectively, were isolated from Euphorbia fischeriana, along with their biogenesis related diterpenoid monomers, 17-hydroxyjolkinolide B (3), caudicifolin (4), and fischeriabietane C (5). Their structures were elucidated by extensive spectroscopy assisted by quantum chemical NMR and ECD calculations. The unusual dimeric skeletons are possibly derived from the adduct of diterpenoid monomers through Michael-like reactions. The novel dimers 1 and 2 exhibited inhibitory activities on soluble epoxide hydrolase (sEH) with IC(50) values of 8.17 and 5.61 microM, respectively. Molecular dynamics studies illustrated that both 1 and 2 can occupy the catalytic pocket of sEH by forming stable hydrogen bonds with the key amino acid residues including Gln384, Asn378, Pro361, Ala365, Asn366, and Asn472.
ESTHER : Zhao_2022_Org.Biomol.Chem__
PubMedSearch : Zhao_2022_Org.Biomol.Chem__
PubMedID: 35266497

Title : Discovery of benzamide derivatives containing urea moiety as soluble epoxide hydrolase inhibitors - Tian_2022_Bioorg.Chem_127_105898
Author(s) : Tian Y , Li S , Dong K , Su X , Fu S , Lv X , Duan M , Yang T , Han Y , Hu G , Liu J , Sun Y , Yue H , Zhang H , Du Z , Miao Z , Tong M , Liu Y , Qin M , Gong P , Hou Y , Gao Z , Zhao Y
Ref : Bioorg Chem , 127 :105898 , 2022
Abstract : The elevation of epoxy-fatty acids through inhibition of soluble epoxide hydrolase (sEH) is efficient for the treatment of inflammatory and pain-related diseases. Herein, we reported the discovery of a series of benzamide derivatives containing urea moiety as sEH inhibitors. Intensive structural modifications led to the identification of compound A34 as a potent sEH inhibitor with good physicochemical properties. Molecular docking revealed an additional hydrogen-bonding interaction between the unique amide scaffold and Phe497, contributing to sEH inhibition potency enhancement. Compound A34 exhibited outstanding inhibitory activity against human sEH, with an IC(50) value of 0.04 +/- 0.01 nM and a K(i) value of 0.2 +/- 0.1 nM. It also showed moderate systemic drug exposure and oral bioavailability in vivo metabolism studies. In carrageenan-induced inflammatory pain rat model, compound A34 exhibited a better therapeutic effect compared to t-AUCB and Celecoxib. Metabolism studies in vivo together with an inflammatory pain evaluation suggest that A34 may be a viable lead compound for the development of highly potent sEH inhibitors.
ESTHER : Tian_2022_Bioorg.Chem_127_105898
PubMedSearch : Tian_2022_Bioorg.Chem_127_105898
PubMedID: 35792317

Title : Synthesis and biological evaluation of new series of benzamide derivatives containing urea moiety as sEH inhibitors - Tian_2022_Bioorg.Med.Chem.Lett_70_128805
Author(s) : Tian Y , Li S , Yang P , Su X , Liu J , Lv X , Dong K , Yang T , Duan M , Hu G , Yue H , Sun Y , Zhang H , Du Z , Miao Z , Tong M , Hou Y , Gao Z , Zhao Y
Ref : Bioorganic & Medicinal Chemistry Lett , 70 :128805 , 2022
Abstract : The pharmacological inhibition of soluble epoxide hydrolase (sEH) was shown to reduce inflammation and pain. Herein, we described a series of newly synthesized sEH inhibitors with the trident-shaped skeleton. Intensive structural modifications led to the identification of compound B15 as a potent sEH inhibitor with an IC(50) value of 0.03 +/- 0.01 nM. Furthermore, compound B15 showed satisfactory metabolic stability in human liver microsomes with a half-time of 197 min. In carrageenan-induced inflammatory pain rat model, compound B15 exhibited a better therapeutic effect compared to t-AUCB and Celecoxib, which demonstrated the proof of potential as anti-inflammatory agents for pain relief.
ESTHER : Tian_2022_Bioorg.Med.Chem.Lett_70_128805
PubMedSearch : Tian_2022_Bioorg.Med.Chem.Lett_70_128805
PubMedID: 35598794

Title : Natural soluble epoxide hydrolase inhibitors from Inula britanica and their potential interactions with soluble epoxide hydrolase: Insight from inhibition kinetics and molecular dynamics - Zhao_2021_Chem.Biol.Interact__109571
Author(s) : Zhao WY , Yan JJ , Zhang M , Wang C , Feng L , Lv X , Huo XK , Sun CP , Chen LX , Ma XC
Ref : Chemico-Biological Interactions , :109571 , 2021
Abstract : Soluble epoxide hydrolase (sEH) is a potential drug target to treat inammation and neurodegenerative diseases. In this study, we found that the extract of Inula britanica exhibited significantly inhibitory effects against sEH, therefore, we investigated its phytochemical constituents to obtain seven new compounds together with sixteen known ones (1-20), including two pairs of novel enantiomers, (2S,3S)-britanicafanin A (1a), (2R,3R)-britanicafanin A (1b), (2R,3S)-britanicafanin B (2a), and (2S,3R)-britanicafanin B (2b), and three new lignans britanicafanins C-E (3-5). Their structures were determined by HRESIMS, 1D and 2D NMR, and electronic circular dichroism (ECD) spectra as well as quantum chemical computations. All the isolates were evaluated for their inhibitory effects against sEH, compounds 1-3, 5-7, 9, 10, 13, 14, and 17-20 showed significant inhibitory effects against sEH with IC(50) values from 3.56 microM to 26.93 microM. The inhibition kinetics results indicated that compounds 9, 10, 13, and 19 were all uncompetitive inhibitors, and their inhibition constants (K(i)) values were 7.11, 1.99, 4.06, and 8.78 microM, respectively. Their potential interactions were analyzed by molecular docking and molecular dynamics (MD), which suggested that amino acid residues Asp335 and Asn359, especially Gln384, played an important role in the inhibition of compounds 10 and 13 on sEH, and compounds 10 and 13 could be considered as the potential candidates for the development of sEH inhibitors.
ESTHER : Zhao_2021_Chem.Biol.Interact__109571
PubMedSearch : Zhao_2021_Chem.Biol.Interact__109571
PubMedID: 34217688

Title : Natural soluble epoxide hydrolase inhibitors from Alisma orientale and their potential mechanism with soluble epoxide hydrolase - Zhao_2021_Int.J.Biol.Macromol_183_811
Author(s) : Zhao WY , Zhang XY , Zhou MR , Tian XG , Lv X , Zhang HL , Deng S , Zhang BJ , Sun CP , Ma XC
Ref : Int J Biol Macromol , 183 :811 , 2021
Abstract : Inhibition of soluble epoxide hydrolase (sEH) is considered to be an effective treatment for inflammation-related diseases, and small molecules origin from natural products show promising activity against sEH. Two undescribed protostanes, 3beta-hydroxy-25-anhydro-alisol F (1) and 3beta-hydroxy-alisol G (2) were isolated from Alisma orientale and identified as new sEH inhibitors with IC(50) values of 10.06 and 30.45 microM, respectively. Potential lead compound 1 was determined as an uncompetitive inhibitor against sEH, which had a K(i) value of 5.13 microM. In-depth molecular docking and molecular dynamics simulations revealed that amino acid residue Ser374 plays an important role in the inhibition of 1, which also provides an idea for the development of sEH inhibitors based on protostane-type triterpenoids.
ESTHER : Zhao_2021_Int.J.Biol.Macromol_183_811
PubMedSearch : Zhao_2021_Int.J.Biol.Macromol_183_811
PubMedID: 33957203

Title : Investigation of the inhibitory effect of protostanes on human carboxylesterase 2 and their interaction: Inhibition kinetics and molecular stimulations - Lv_2021_Int.J.Biol.Macromol_167_1262
Author(s) : Lv X , Bai R , Yan JK , Huang HL , Huo XK , Tian XG , Zhao XY , Zhang BJ , Zhao WY , Sun CP
Ref : Int J Biol Macromol , 167 :1262 , 2021
Abstract : Carboxylesterase 2 (CES 2), plays a pivotal role in endobiotic homeostasis and xenobiotic metabolism. Protostanes, the major constituents of the genus Alisma, display a series of pharmacological activities. Despite the extensive studies of pharmacological activities, the investigation on inhibitory effects of protostanes against CES 2 is rarely reported. In this study, the inhibitory activities of a library of protostanes (1-25) against human CES 2 were investigated for the first time, using 6,8-dichloro-9,9-dimethyl-7-oxo-7,9-dihydroacridin-2-yl benzoate (DDAB) as the specific fluorescent probe for human CES 2. Compounds 1, 2, 7, 8, 12, 13, 18, 19, and 25 showed strong inhibitory effects towards CES 2. For the most potent compounds 1, 7, 13, and 25, the inhibition kinetics were further investigated, and these four protostanes were all uncompetitive inhibitors against human CES 2 with the inhibition constant (K(i)) values ranging from 0.89 microM to 2.83 microM. In addition, molecular docking and molecular dynamics stimulation were employed to analyze the potential interactions between these protostanes and CES 2, and amino acid residue Gln422 was identified to play a crucial role in the strong inhibition of protostanes towards CES 2.
ESTHER : Lv_2021_Int.J.Biol.Macromol_167_1262
PubMedSearch : Lv_2021_Int.J.Biol.Macromol_167_1262
PubMedID: 33189757

Title : Neuregulin-1beta Protects the Rat Diaphragm during Sepsis against Oxidative Stress and Inflammation by Activating the PI3K\/Akt Pathway - Liu_2020_Oxid.Med.Cell.Longev_2020_1720961
Author(s) : Liu H , Weng XJ , Yao JY , Zheng J , Lv X , Zhou XH , Jiang H , Li ST
Ref : Oxid Med Cell Longev , 2020 :1720961 , 2020
Abstract : Sepsis-induced diaphragm dysfunction (SIDD) which is mainly characterized by decrease in diaphragmatic contractility has been identified to cause great harms to patients. Therefore, there is an important and pressing need to find effective treatments for improving SIDD. In addition, acetylcholinesterase (AChE) activity is a vital property of the diaphragm, so we evaluated both diaphragmatic contractility and AChE activity. Though neuregulin-1beta (NRG-1beta) is known to exert organ-protective effects in some inflammatory diseases, little is known about the potential of NRG-1beta therapy in the diaphragm during sepsis. Our study was aimed at exploring the effects of NRG-1beta application on diaphragmatic contractility and AChE activity during sepsis. Proinflammatory cytokines, muscle injury biomarkers in serum, contractile force, AChE activity, proinflammatory cytokines, oxidative parameters, histological condition, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, and expression of phosphoinositide 3-kinase (PI3K)/protein kinase B (PKB/Akt) signaling proteins in the diaphragm were measured and compared between nonseptic and septic groups with or without NRG-1beta treatment. In vitro, the effects of NRG-1beta on reactive oxygen species (ROS) production in the lipopolysaccharide- (LPS-) stimulated L6 rat muscle skeletal cells with or without the Akt inhibitor MK-2206 were detected. NRG-1beta inhibited proinflammatory cytokine release and muscle injury biomarkers soaring in serum and improved the sepsis-induced diaphragm dysfunction and AChE activity decrease significantly during sepsis. Meanwhile, the inflammatory response, oxidative stress, pathological impairment, and cell apoptosis in the diaphragm were mitigated by NRG-1beta. And NRG-1beta activated the PI3K/Akt signaling in the diaphragm of septic rats. Elevated ROS production in the LPS-stimulated L6 rat skeletal muscle cells was reduced after treatment with NRG-1beta, while MK-2206 blocked these effects of NRG-1beta. In conclusion, our findings underlined that NRG-1beta could reduce circulating levels of proinflammatory cytokines in rats with sepsis, adjust diaphragmatic proinflammatory cytokine level, mitigate diaphragmatic oxidative injury, and lessen diaphragm cell apoptosis, thereby improving diaphragmatic function, and play a role in diaphragmatic protection by activating PI3K/Akt signaling.
ESTHER : Liu_2020_Oxid.Med.Cell.Longev_2020_1720961
PubMedSearch : Liu_2020_Oxid.Med.Cell.Longev_2020_1720961
PubMedID: 32765805

Title : Enhancing secretion of polyethylene terephthalate hydrolase PETase in Bacillus subtilis WB600 mediated by the SP(amy) signal peptide - Wang_2020_Lett.Appl.Microbiol__
Author(s) : Wang N , Guan F , Lv X , Han D , Zhang Y , Wu N , Xia X , Tian J
Ref : Lett Appl Microbiol , : , 2020
Abstract : The polyethylene terephthalate hydrolase (PETase) has been proved to have a high activity to degrade polyethylene terephthalate (PET), but few studies have been carried on its secretion in Bacillus subtilis. In this study, the coding gene of PETase, which was isolated from the Ideonella sakaiensis, was synthesized and expressed in B. subtilis. Then, we evaluated the ability of five Bacillus signal peptides to enhance PETase secretion by B. subtilis. The results indicated that the SP(amy) -induced secretion of PETase was the highest, and its activity against p-Nitrophenyl palmitate was about fourfold that of the natural signal peptide SP(PETase) . The weak promoter P43 provided sufficient time for translation and folding of PETase, resulting in increased extracellular expression. Use of P43 and SP(amy) in combination yielded the greatest bis-(2-hydroxyethyl) terephthalate degradation and PET-film etching activity due to maximized secretion of PETase by B. subtilis. Our findings will facilitate biodegradation of PET plastic.
ESTHER : Wang_2020_Lett.Appl.Microbiol__
PubMedSearch : Wang_2020_Lett.Appl.Microbiol__
PubMedID: 32394501

Title : Expression of ADAM29 and FAM135B in the pathological evolution from normal esophageal epithelium to esophageal cancer: Their differences and clinical significance - Wang_2020_Oncol.Lett_19_1727
Author(s) : Wang T , Lv X , Jiang S , Han S , Wang Y
Ref : Oncol Lett , 19 :1727 , 2020
Abstract : A Disintegrin And Metalloprotease Domain 29 (ADAM29) and Family with sequence similarity 135 member B (FAM135B) genes have been reported to be associated with a carcinogenic risk of esophageal squamous cell carcinoma (ESCC). However, to the best of our knowledge, the expression of ADAM29 and FAM135B in the pathological evolution from normal esophageal epithelial cells to ESCC has not yet been investigated. The present study aimed to investigate the expression of ADAM29 and FAM135B in normal esophageal mucosal epithelium, low-grade and high-grade esophageal intraepithelial neoplasia, and ESCC. Furthermore, the present study aimed to investigate the role of ADAM29 and FAM135B in the development of esophageal lesions. Immunohistochemistry was performed in order to detect the expression levels of ADAM29 and FAM135B proteins in normal esophageal mucosa samples (40 cases), low-grade intraepithelial neoplasia samples (20 cases), high-grade intraepithelial neoplasia samples (20 cases) and ESCC samples (40 cases). The results of the present study demonstrated that the positive rates of ADAM29 and FAM135B proteins increased gradually from normal esophageal mucosal epithelium and esophageal intraepithelial neoplasia, to ESCC (P<0.05). Furthermore, the expression levels of ADAM29 and FAM135B proteins in ESCC were not associated with age and the tumor size (P>0.05); however, the protein levels were associated with the pathological stage, clinical stage and lymph node metastasis of ESCC (P<0.05). In addition, there was a significant association between the expression levels of ADAM29 protein and FAM135B protein ((2)=60.071; P<0.001). The results of the present study demonstrated that the expression levels of ADAM29 and FAM135B were associated with the tumor behavior characteristics and the progression of esophageal cancer, the expression of which could be used for the diagnosis of early esophageal cancer, and provide the basis for guiding individualized treatment.
ESTHER : Wang_2020_Oncol.Lett_19_1727
PubMedSearch : Wang_2020_Oncol.Lett_19_1727
PubMedID: 32194665
Gene_locus related to this paper: human-FAM135B

Title : Determination of Genetic Effects of LIPK and LIPJ Genes on Milk Fatty Acids in Dairy Cattle - Shi_2019_Genes.(Basel)_10_
Author(s) : Shi L , Han B , Liu L , Lv X , Ma Z , Li C , Xu L , Li Y , Zhao F , Yang Y , Sun D
Ref : Genes (Basel) , 10 : , 2019
Abstract : In our previous genome-wide association study (GWAS) on milk fatty acids (FAs) in Chinese Holstein, we discovered 83 genome-wide significant single nucleotide polymorphisms (SNPs) associated with milk FAs. Two of them were close to lipase family member K (LIPK) and lipase family member J (LIPJ), respectively. Hence, this study is a follow-up to verify whether the LIPK and LIPJ have significant genetic effects on milk FAs in dairy cattle. By re-sequencing the entire exons, and 3 kb of 5' and 3' flanking regions, two and seven SNPs were identified in LIPK and LIPJ, respectively, including a novel SNP, ss158213049726. With the Haploview 4.1 software, we found that five of the SNPs in LIPJ formed a haplotype block (D' = 0.96 ~ 1.00). Single-locus association analyses revealed that each SNP in LIPK and LIPJ was significantly associated with at least one milk FA (p = < 1.00x10(-4) ~ 4.88x10(-2)), and the haplotype-based association analyses showed significant genetic effects on nine milk FAs (p = < 1.00x10(-4) ~ 3.98x10(-2)). Out of these SNPs, the missense mutation in LIPK gene, rs42774527, could change the protein secondary structure and function predicted by SOPMA, SIFT, and PROVEAN softwares. With the Genomatix software, we predicted that two SNPs, rs110322221 in LIPK and rs211373799 in LIPJ, altered the transcription factors binding sites (TFBSs), indicating their potential regulation on promoter activity of the genes. Furthermore, we found that both LIPK and LIPJ had relatively high expressions in the mammary gland. In conclusion, our research is the first to demonstrate that LIPK and LIPJ genes have significant associations with milk FAs, and the identified SNPs might be served as genetic markers to optimize breeding programs for milk FAs in dairy cattle. This research deserves in-depth verification.
ESTHER : Shi_2019_Genes.(Basel)_10_
PubMedSearch : Shi_2019_Genes.(Basel)_10_
PubMedID: 30696079
Gene_locus related to this paper: bovin-e1bnt1 , bovin-f1msa3 , human-LIPJ , human-LIPK

Title : Down-regulation of fibronectin and the correlated expression of neuroligin in hirschsprung disease - Zheng_2017_Neurogastroenterol.Motil_29_
Author(s) : Zheng Y , Lv X , Wang D , Gao N , Zhang Q , Li A
Ref : Neurogastroenterol Motil , 29 : , 2017
Abstract : AIM: The goal of this study was to investigate the expression of fibronectin (FN) and the correlated abundance of neuroligins (NLs) in the enteric nervous system (ENS) and to find a novel diagnostic marker in the serum of Hirschsprung disease (HSCR) patients. METHODS: The expression levels of FN, neuroligin-1 and neuroligin-2 were detected in 114 children with or without HSCR. The expression and localization of the NLs and FN were assessed morphologically by immunohistochemical staining. Western blot analysis and real-time fluorescence quantitative PCR (qPCR) were performed to examine the correlated expression of the NLs and FN in aganglionic, transitional, and normal ganglionic colon tissues. An enzyme-linked immunosorbent assay (ELISA) was performed to evaluate and compare serum FN levels between HSCR and non-HSCRand between long-type HSCR and short-type HSCR. RESULTS: These studies showed that both neuroligin-1 and neuroligin-2 were expressed at low levels in aganglionic segments and at intermediate levels in transitional segments compared to their high level of expression in normal tissue. In contrast, FN expression was negatively correlated, with expression in these three samples transitioning from highest to lowest. The serum FN level was higher in HSCR than in non-HSCR, but no significant difference between short-type HSCR and long-type HSCR was observed. CONCLUSION: FN affects the expression of both neuroligin-1 and neuroligin-2 in HSCR, which may lead to the hypoplasia of ganglion cells in the ENS. This correlation may play a key role in the pathogenesis, diagnosis, or classification of HSCR.
ESTHER : Zheng_2017_Neurogastroenterol.Motil_29_
PubMedSearch : Zheng_2017_Neurogastroenterol.Motil_29_
PubMedID: 28656720

Title : A bioluminescent sensor for highly selective and sensitive detection of human carboxylesterase 1 in complex biological samples - Wang_2016_Chem.Commun.(Camb)_52_3183
Author(s) : Wang DD , Jin Q , Zou LW , Hou J , Lv X , Lei W , Cheng HL , Ge GB , Yang L
Ref : Chem Commun (Camb) , 52 :3183 , 2016
Abstract : A highly selective and sensitive bioluminescent sensor (DME) for human carboxylesterase 1 (hCE1) has been developed and well characterized. DME could be used for real-time monitoring of hCE1 activities in complex biological samples and for bio-imaging of endogenous hCE1 in living cells.
ESTHER : Wang_2016_Chem.Commun.(Camb)_52_3183
PubMedSearch : Wang_2016_Chem.Commun.(Camb)_52_3183
PubMedID: 26809686

Title : Ultrasonic pretreatment in lipase-catalyzed synthesis of structured lipids with high 1,3-dioleoyl-2-palmitoylglycerol content - Liu_2015_Ultrason.Sonochem_23_100
Author(s) : Liu SL , Dong XY , Wei F , Wang X , Lv X , Zhong J , Wu L , Quek SY , Chen H
Ref : Ultrason Sonochem , 23 :100 , 2015
Abstract : Production of structured lipid 1,3-dioleoyl-2-palmitoylglycerol (OPO), from tripalmitin (PPP) and oleic acid (OA) using lipases and ultrasonic pretreatment was conducted. Factors influencing both the ultrasonic conditions and enzymatic reaction were investigated. Optimum conditions could be attained with 6 min pretreatment time, 50% ultrasonic power, 3 s/9 s (work/pause) cycle of ultrasonic pulse, 1:8 PPP/OA molar ratio, 12% enzyme dosage and 50 degreeC temperature of. At the optimum conditions, the OPO yield of 51.8% could be achieved in 4h. Studies showed that the OPO content increased to 35.9% in 1h with ultrasonic pretreatment, in comparison to 4h without ultrasonic pretreatment. Reuse of Lipozyme RM IM for 10 cycles under ultrasonic irradiation did not cause essential damage to its lipase activity. Reaction kinetic model fitted well with the proposed Ping-Pong mechanism. The apparent kinetic constant (Vm'/K) of ultrasound pretreatment reaction was 2.52 times higher than the conventional mechanical stirring, indicating that ultrasound pretreatment enhanced the substrates affinity to the enzyme. This study confirmed that ultrasonic pretreatment was more efficient in OPO production than conventional mechanical agitation.
ESTHER : Liu_2015_Ultrason.Sonochem_23_100
PubMedSearch : Liu_2015_Ultrason.Sonochem_23_100
PubMedID: 25453210

Title : Vagal modulation of high mobility group box-1 protein mediates electroacupuncture-induced cardioprotection in ischemia-reperfusion injury - Zhang_2015_Sci.Rep_5_15503
Author(s) : Zhang J , Yong Y , Li X , Hu Y , Wang J , Wang YQ , Song W , Chen WT , Xie J , Chen XM , Lv X , Hou LL , Wang K , Zhou J , Wang XR , Song JG
Ref : Sci Rep , 5 :15503 , 2015
Abstract : Excessive release of high mobility group box-1 (HMGB1) protein from ischemic cardiomyocytes activates inflammatory cascades and enhances myocardial injury after reperfusion. Here we report evidence that electroacupuncture of mice at Neiguan acupoints can inhibit the up-regulation of cardiac HMGB1 following myocardial ischemia and attenuate the associated inflammatory responses and myocardial injury during reperfusion. These benefits of electroacupuncture were partially reversed by administering recombinant HMGB1 to the mice, and further potentiated by administering anti-HMGB1 antibody. Electroacupuncture-induced inhibition of HMGB1 release was markedly reduced by unilateral vagotomy or administration of nicotinic receptor antagonist, but not by chemical sympathectomy. The cholinesterase inhibitor neostigmine mimicked the effects of electroacupuncture on HMGB1 release and myocardial ischemia reperfusion injury. Culture experiments with isolated neonatal cardiomyocytes showed that acetylcholine, but not noradrenaline, inhibited hypoxia-induced release of HMGB1 via a alpha7nAchR-dependent pathway. These results suggest that electroacupuncture acts via the vagal nerve and its nicotinic receptor-mediated signaling to inhibit HMGB1 release from ischemic cardiomyocytes. This helps attenuate pro-inflammatory responses and myocardial injury during reperfusion.
ESTHER : Zhang_2015_Sci.Rep_5_15503
PubMedSearch : Zhang_2015_Sci.Rep_5_15503
PubMedID: 26499847

Title : Fructus Psoraleae contains natural compounds with potent inhibitory effects towards human carboxylesterase 2 - Li_2015_Fitoterapia_101_99
Author(s) : Li YG , Hou J , Li SY , Lv X , Ning J , Wang P , Liu ZM , Ge GB , Ren JY , Yang L
Ref : Fitoterapia , 101 :99 , 2015
Abstract : Fructus Psoraleae (FP) is an edible Chinese herbal which is widely used in Asia for the treatment of various diseases including asthma, diarrhea, and osteoporosis. This study aimed to investigate the inhibitory effects of the crude ethanol extract from FP on human carboxylesterase 2 (hCE2), as well as to identity and characterize the naturally occurring inhibitors of hCE2 in FP. Our results demonstrated that the ethanol extract of FP displayed potent inhibitory effects towards hCE2, while five major bioactive constitutes in FP were efficiently identified by LC-DAD-ESI-MS/MS, with the aid of LC-based activity profiling. The identified bioactive compounds including neobavaisoflavone, isobavachalcone, bavachinin, corylifol A and bakuchiol were found to be naturally occurring potent inhibitors of hCE2, with low Ki values ranging from 0.62muM to 3.89muM. This is the first report of the chemical constitutes in FP as potent inhibitors of hCE2.
ESTHER : Li_2015_Fitoterapia_101_99
PubMedSearch : Li_2015_Fitoterapia_101_99
PubMedID: 25596095

Title : A highly selective ratiometric fluorescent probe for in vitro monitoring and cellular imaging of human carboxylesterase 1 - Liu_2014_Biosens.Bioelectron_57C_30
Author(s) : Liu ZM , Feng L , Ge GB , Lv X , Hou J , Cao YF , Cui JN , Yang L
Ref : Biosensors & Bioelectronics , 57C :30 , 2014
Abstract : A new ratiometric fluorescent probe derived from 2-(2-hydroxy-3-methoxyphenyl) benzothiazole (HMBT) has been developed for selective monitoring of human carboxylesterase 1 (hCE1). The probe is designed by introducing benzoyl moiety to HMBT. The prepared latent spectroscopic probe 1 displays satisfying stability under physiological pH conditions with very low background signal. Both the reaction phynotyping and chemical inhibition assays demonstrated that hCE1 mediated the specific cleavage of the carboxylic ester bond of probe 1 in human biological samples. The release of HMBT leads to a remarkable red-shifted emission in fluorescence spectrum (120nm large emission shift). Furthermore, human cell-based assays show that probe 1 is cell membrane permeable, and it can be used for bioassay and cellular imaging of hCE1 activity in HepG2 cells. These findings lead to the development of a simple and sensitive fluorescent method for measurement of hCE1 activity in vitro or in living cells, in the presence of additional enzymes or endogenous compounds.
ESTHER : Liu_2014_Biosens.Bioelectron_57C_30
PubMedSearch : Liu_2014_Biosens.Bioelectron_57C_30
PubMedID: 24534577

Title : A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells, - Liu_2014_Sens.Actuators.B.Chem_205_151
Author(s) : Liu ZM , Feng L , Hou J , Lv X , Ning J , Ge GB , Wang KW , Cui JN , Yang L
Ref : Sensors and Actuators B: Chemical , 205 :151 , 2014
Abstract : A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated.
ESTHER : Liu_2014_Sens.Actuators.B.Chem_205_151
PubMedSearch : Liu_2014_Sens.Actuators.B.Chem_205_151

Title : A highly selective long-wavelength fluorescent probe for the detection of human carboxylesterase 2 and its biomedical applications - Feng_2014_Chem.Commun.(Camb)_50_14519
Author(s) : Feng L , Liu ZM , Xu L , Lv X , Ning J , Hou J , Ge GB , Cui JN , Yang L
Ref : Chem Commun (Camb) , 50 :14519 , 2014
Abstract : A highly selective long-wavelength fluorescent probe TCFB has been developed for the detection of hCE2. The probe can be used for real-time monitoring of hCE2 activity in complex biological systems.
ESTHER : Feng_2014_Chem.Commun.(Camb)_50_14519
PubMedSearch : Feng_2014_Chem.Commun.(Camb)_50_14519
PubMedID: 25303144
Gene_locus related to this paper: human-CES2

Title : A highly selective fluorescent ESIPT probe for the detection of Human carboxylesterase 2 and its biological applications - Feng_2014_Biosens.Bioelectron_65C_9
Author(s) : Feng L , Liu ZM , Hou J , Lv X , Ning J , Ge GB , Cui JN , Yang L
Ref : Biosensors & Bioelectronics , 65C :9 , 2014
Abstract : A new ratiometric florescence probe derived from 3-hydroxyflavone (3-HF) has been developed for selective and sensitive detection of human carboxylesterase 2 (CE2). The probe is designed by modulating the excited state intramolecular proton transfer (ESIPT) emission of 3-HF via introducing of 4-ethylbenzoyloxy group. Under physiological conditions, probe 1 displays satisfying stability with very low background signal, but it can be selectively hydrolyzed by CE2 to release free 3-HF which brings remarkable changes in fluorescence spectrum. Both reaction phenotyping and chemical inhibition assays demonstrate that probe 1 is highly selective for CE2 over other human hydrolases including carboxylesterase 1, cholinesterases and paraoxonases. Probe 1 has been applied successfully to measure the real activities of CE2 in human biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. Additionally, probe 1 is cell membrane permeable and can be used for cellular imaging of endogenous CE2 in living cells. All of these features make it possible to serve as a promising tool for exploring the individual differences in biological function of CE2, as well as for rapid screening of selective and potent inhibitors of CE2 for further clinical use.
ESTHER : Feng_2014_Biosens.Bioelectron_65C_9
PubMedSearch : Feng_2014_Biosens.Bioelectron_65C_9
PubMedID: 25461132

Title : Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization - Qin_2014_Proc.Natl.Acad.Sci.U.S.A_111_5135
Author(s) : Qin C , Yu C , Shen Y , Fang X , Chen L , Min J , Cheng J , Zhao S , Xu M , Luo Y , Yang Y , Wu Z , Mao L , Wu H , Ling-Hu C , Zhou H , Lin H , Gonzalez-Morales S , Trejo-Saavedra DL , Tian H , Tang X , Zhao M , Huang Z , Zhou A , Yao X , Cui J , Li W , Chen Z , Feng Y , Niu Y , Bi S , Yang X , Cai H , Luo X , Montes-Hernandez S , Leyva-Gonzalez MA , Xiong Z , He X , Bai L , Tan S , Liu D , Liu J , Zhang S , Chen M , Zhang L , Zhang Y , Liao W , Wang M , Lv X , Wen B , Liu H , Luan H , Yang S , Wang X , Xu J , Li X , Li S , Wang J , Palloix A , Bosland PW , Li Y , Krogh A , Rivera-Bustamante RF , Herrera-Estrella L , Yin Y , Yu J , Hu K , Zhang Z
Ref : Proc Natl Acad Sci U S A , 111 :5135 , 2014
Abstract : As an economic crop, pepper satisfies people's spicy taste and has medicinal uses worldwide. To gain a better understanding of Capsicum evolution, domestication, and specialization, we present here the genome sequence of the cultivated pepper Zunla-1 (C. annuum L.) and its wild progenitor Chiltepin (C. annuum var. glabriusculum). We estimate that the pepper genome expanded approximately 0.3 Mya (with respect to the genome of other Solanaceae) by a rapid amplification of retrotransposons elements, resulting in a genome comprised of approximately 81% repetitive sequences. Approximately 79% of 3.48-Gb scaffolds containing 34,476 protein-coding genes were anchored to chromosomes by a high-density genetic map. Comparison of cultivated and wild pepper genomes with 20 resequencing accessions revealed molecular footprints of artificial selection, providing us with a list of candidate domestication genes. We also found that dosage compensation effect of tandem duplication genes probably contributed to the pungent diversification in pepper. The Capsicum reference genome provides crucial information for the study of not only the evolution of the pepper genome but also, the Solanaceae family, and it will facilitate the establishment of more effective pepper breeding programs.
ESTHER : Qin_2014_Proc.Natl.Acad.Sci.U.S.A_111_5135
PubMedSearch : Qin_2014_Proc.Natl.Acad.Sci.U.S.A_111_5135
PubMedID: 24591624
Gene_locus related to this paper: capch-q75qh4 , capan-a0a1u8fuf5 , capan-a0a1u8gmz3 , capan-a0a1u8f879 , capan-a0a1u8ftr2 , capan-a0a1u8g8s6

Title : Genome sequence of Acinetobacter baumannii MDR-TJ - Gao_2011_J.Bacteriol_193_2365
Author(s) : Gao F , Wang Y , Liu YJ , Wu XM , Lv X , Gan YR , Song SD , Huang H
Ref : Journal of Bacteriology , 193 :2365 , 2011
Abstract : Acinetobacter baumannii is a pathogenic species of bacteria, identified as an aerobic gram-negative bacterium, that is resistant to most antibiotics. In this study, the MDR-TJ strain was isolated at the Second Hospital of Tianjin Medical University, China, and was found to be resistant to penicillin, cephalosporins, aminoglycosides, quinolones, and also imipenem. The genome sequence of Acinetobacter baumannii strain MDR-TJ was determined by using a combination of 454 pyrosequencing and paired-end sequencing performed with the Roche Genome Sequencer FLX system to generate a scaffolded assembly.
ESTHER : Gao_2011_J.Bacteriol_193_2365
PubMedSearch : Gao_2011_J.Bacteriol_193_2365
PubMedID: 21398552
Gene_locus related to this paper: aciba-f5iht4 , aciba-a0a009wzt4

Title : The draft genome of the carcinogenic human liver fluke Clonorchis sinensis - Wang_2011_Genome.Biol_12_R107
Author(s) : Wang X , Chen W , Huang Y , Sun J , Men J , Liu H , Luo F , Guo L , Lv X , Deng C , Zhou C , Fan Y , Li X , Huang L , Hu Y , Liang C , Hu X , Xu J , Yu X
Ref : Genome Biol , 12 :R107 , 2011
Abstract : BACKGROUND: Clonorchis sinensis is a carcinogenic human liver fluke that is widespread in Asian countries. Increasing infection rates of this neglected tropical disease are leading to negative economic and public health consequences in affected regions. Experimental and epidemiological studies have shown a strong association between the incidence of cholangiocarcinoma and the infection rate of C. sinensis. To aid research into this organism, we have sequenced its genome. RESULTS: We combined de novo sequencing with computational techniques to provide new information about the biology of this liver fluke. The assembled genome has a total size of 516 Mb with a scaffold N50 length of 42 kb. Approximately 16,000 reliable protein-coding gene models were predicted. Genes for the complete pathways for glycolysis, the Krebs cycle and fatty acid metabolism were found, but key genes involved in fatty acid biosynthesis are missing from the genome, reflecting the parasitic lifestyle of a liver fluke that receives lipids from the bile of its host. We also identified pathogenic molecules that may contribute to liver fluke-induced hepatobiliary diseases. Large proteins such as multifunctional secreted proteases and tegumental proteins were identified as potential targets for the development of drugs and vaccines. CONCLUSIONS: This study provides valuable genomic information about the human liver fluke C. sinensis and adds to our knowledge on the biology of the parasite. The draft genome will serve as a platform to develop new strategies for parasite control.
ESTHER : Wang_2011_Genome.Biol_12_R107
PubMedSearch : Wang_2011_Genome.Biol_12_R107
PubMedID: 22023798
Gene_locus related to this paper: closi-h2krw6