Rincones J

References (2)

Title : The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential - Parizzi_2012_BMC.Genomics_13_562
Author(s) : Parizzi LP , Grassi MC , Llerena LA , Carazzolle MF , Queiroz VL , Lunardi I , Zeidler AF , Teixeira PJ , Mieczkowski P , Rincones J , Pereira GA
Ref : BMC Genomics , 13 :562 , 2012
Abstract : BACKGROUND: Synthetic biology allows the development of new biochemical pathways for the production of chemicals from renewable sources. One major challenge is the identification of suitable microorganisms to hold these pathways with sufficient robustness and high yield. In this work we analyzed the genome of the propionic acid producer Actinobacteria Propionibacterium acidipropionici (ATCC 4875).
RESULTS: The assembled P. acidipropionici genome has 3,656,170 base pairs (bp) with 68.8% G + C content and a low-copy plasmid of 6,868 bp. We identified 3,336 protein coding genes, approximately 1000 more than P. freudenreichii and P. acnes, with an increase in the number of genes putatively involved in maintenance of genome integrity, as well as the presence of an invertase and genes putatively involved in carbon catabolite repression. In addition, we made an experimental confirmation of the ability of P. acidipropionici to fix CO2, but no phosphoenolpyruvate carboxylase coding gene was found in the genome. Instead, we identified the pyruvate carboxylase gene and confirmed the presence of the corresponding enzyme in proteome analysis as a potential candidate for this activity. Similarly, the phosphate acetyltransferase and acetate kinase genes, which are considered responsible for acetate formation, were not present in the genome. In P. acidipropionici, a similar function seems to be performed by an ADP forming acetate-CoA ligase gene and its corresponding enzyme was confirmed in the proteome analysis.
CONCLUSIONS: Our data shows that P. acidipropionici has several of the desired features that are required to become a platform for the production of chemical commodities: multiple pathways for efficient feedstock utilization, ability to fix CO2, robustness, and efficient production of propionic acid, a potential precursor for valuable 3-carbon compounds.
ESTHER : Parizzi_2012_BMC.Genomics_13_562
PubMedSearch : Parizzi_2012_BMC.Genomics_13_562
PubMedID: 23083487
Gene_locus related to this paper: proa4-k7rwz8

Title : A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao - Mondego_2008_BMC.Genomics_9_548
Author(s) : Mondego JM , Carazzolle MF , Costa GG , Formighieri EF , Parizzi LP , Rincones J , Cotomacci C , Carraro DM , Cunha AF , Carrer H , Vidal RO , Estrela RC , Garcia O , Thomazella DP , de Oliveira BV , Pires AB , Rio MC , Araujo MR , de Moraes MH , Castro LA , Gramacho KP , Goncalves MS , Neto JP , Neto AG , Barbosa LV , Guiltinan MJ , Bailey BA , Meinhardt LW , Cascardo JC , Pereira GA
Ref : BMC Genomics , 9 :548 , 2008
Abstract : BACKGROUND: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9x coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. RESULTS: Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. CONCLUSION: This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/cacao pathosystem.
ESTHER : Mondego_2008_BMC.Genomics_9_548
PubMedSearch : Mondego_2008_BMC.Genomics_9_548
PubMedID: 19019209
Gene_locus related to this paper: monpe-e2l806 , monpe-e2lyh7 , monpe-e2lz46 , monro-v2x3v0 , monro-v2yx67 , monpe-e2lnh8