Thomazella DP

References (2)

Title : Genome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthora roreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phases - Meinhardt_2014_BMC.Genomics_15_164
Author(s) : Meinhardt LW , Costa GG , Thomazella DP , Teixeira PJ , Carazzolle MF , Schuster SC , Carlson JE , Guiltinan MJ , Mieczkowski P , Farmer A , Ramaraj T , Crozier J , Davis RE , Shao J , Melnick RL , Pereira GA , Bailey BA
Ref : BMC Genomics , 15 :164 , 2014
Abstract : BACKGROUND: The basidiomycete Moniliophthora roreri is the causal agent of Frosty pod rot (FPR) disease of cacao (Theobroma cacao), the source of chocolate, and FPR is one of the most destructive diseases of this important perennial crop in the Americas. This hemibiotroph infects only cacao pods and has an extended biotrophic phase lasting up to sixty days, culminating in plant necrosis and sporulation of the fungus without the formation of a basidiocarp.
RESULTS: We sequenced and assembled 52.3 Mb into 3,298 contigs that represent the M. roreri genome. Of the 17,920 predicted open reading frames (OFRs), 13,760 were validated by RNA-Seq. Using read count data from RNA sequencing of cacao pods at 30 and 60 days post infection, differential gene expression was estimated for the biotrophic and necrotrophic phases of this plant-pathogen interaction. The sequencing data were used to develop a genome based secretome for the infected pods. Of the 1,535 genes encoding putative secreted proteins, 1,355 were expressed in the biotrophic and necrotrophic phases. Analysis of the data revealed secretome gene expression that correlated with infection and intercellular growth in the biotrophic phase and invasive growth and plant cellular death in the necrotrophic phase.
CONCLUSIONS: Genome sequencing and RNA-Seq was used to determine and validate the Moniliophthora roreri genome and secretome. High sequence identity between Moniliophthora roreri genes and Moniliophthora perniciosa genes supports the taxonomic relationship with Moniliophthora perniciosa and the relatedness of this fungus to other basidiomycetes. Analysis of RNA-Seq data from infected plant tissues revealed differentially expressed genes in the biotrophic and necrotrophic phases. The secreted protein genes that were upregulated in the biotrophic phase are primarily associated with breakdown of the intercellular matrix and modification of the fungal mycelia, possibly to mask the fungus from plant defenses. Based on the transcriptome data, the upregulated secreted proteins in the necrotrophic phase are hypothesized to be actively attacking the plant cell walls and plant cellular components resulting in necrosis. These genes are being used to develop a new understanding of how this disease interaction progresses and to identify potential targets to reduce the impact of this devastating disease.
ESTHER : Meinhardt_2014_BMC.Genomics_15_164
PubMedSearch : Meinhardt_2014_BMC.Genomics_15_164
PubMedID: 24571091
Gene_locus related to this paper: monro-v2wn76 , monro-v2xuz8 , monro-v2xl67 , monro-v2xnp4 , monro-v2wv67 , monro-v2wja9

Title : A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao - Mondego_2008_BMC.Genomics_9_548
Author(s) : Mondego JM , Carazzolle MF , Costa GG , Formighieri EF , Parizzi LP , Rincones J , Cotomacci C , Carraro DM , Cunha AF , Carrer H , Vidal RO , Estrela RC , Garcia O , Thomazella DP , de Oliveira BV , Pires AB , Rio MC , Araujo MR , de Moraes MH , Castro LA , Gramacho KP , Goncalves MS , Neto JP , Neto AG , Barbosa LV , Guiltinan MJ , Bailey BA , Meinhardt LW , Cascardo JC , Pereira GA
Ref : BMC Genomics , 9 :548 , 2008
Abstract : BACKGROUND: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9x coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. RESULTS: Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. CONCLUSION: This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/cacao pathosystem.
ESTHER : Mondego_2008_BMC.Genomics_9_548
PubMedSearch : Mondego_2008_BMC.Genomics_9_548
PubMedID: 19019209
Gene_locus related to this paper: monpe-e2l806 , monpe-e2lyh7 , monpe-e2lz46 , monro-v2x3v0 , monro-v2yx67 , monpe-e2lnh8