Cunha AF

References (4)

Title : Atropine counteracts the depressive-like behaviour elicited by acute exposure to commercial chlorpyrifos in rats - Siqueira_2019_Neurotoxicol.Teratol_71_6
Author(s) : Siqueira AA , Cunha AF , Marques GLM , Felippe ISA , Minassa VS , Gramelich T , Cicilini MA , Alarcon TA , Pires RGW , Sampaio KN , Beijamini V
Ref : Neurotoxicology & Teratology , 71 :6 , 2019
Abstract : Acute organophosphate (OP) poisoning induces well-known signs of toxicosis related to acetylcholinesterase (AChE) inhibition. However, the relationship between acute OP poisoning and the onset of psychiatric disorders remains unclear. Thus, we investigated behavioural and biochemical consequences of acute exposure to the OP chlorpyrifos in male rats and also the effectiveness of the antidotes atropine and pralidoxime on reversing these changes. A sub-lethal dose of commercial chlorpyrifos (20mg/kg, i.p.) elicited signs of acute toxicosis during the first hours after its injection in rats. Twenty-four hours after treatment, this single dose of chlorpyrifos induced a depressive-like behaviour in the rat forced swimming test without impairing locomotor activity. At this time (24h), chlorpyrifos decreased plasma butyrylcholinesterase (BChE) activity and hippocampal, striatal and prefrontal cortical AChE activity in rats. The behavioural and biochemical consequences of acute chlorpyrifos poisoning do not seem to be long lasting, since 30days later they were absent. We evaluated whether these behavioural and biochemical consequences of acute chlorpyrifos treatment would be reversed by the antidotes atropine (10mg/kgi.p.) and/or pralidoxime (40mg/kg; i.p.) given 1h after poisoning. Pralidoxime partially reactivated the AChE activity in the prefrontal cortex, but not in the hippocampus and striatum. Atropine attenuated the depressive-like behaviour induced by chlorpyrifos in rats. Our results suggest that acute chlorpyrifos poisoning induces a transient depressive-like behaviour possible related to hippocampal AChE inhibition. They suggest that treatment with atropine and pralidoxime seems to be insufficient to counteract all the effects of OP acute poisoning, at least in rats.
ESTHER : Siqueira_2019_Neurotoxicol.Teratol_71_6
PubMedSearch : Siqueira_2019_Neurotoxicol.Teratol_71_6
PubMedID: 30458229

Title : Neuroreflex control of cardiovascular function is impaired after acute poisoning with chlorpyrifos, an organophosphorus insecticide: Possible short and long term clinical implications - Cunha_2018_Toxicology_398-399_13
Author(s) : Cunha AF , Felippe ISA , Ferreira-Junior NC , Resstel LBM , Guimaraes DAM , Beijamini V , Paton JFR , Sampaio KN
Ref : Toxicology , 398-399 :13 , 2018
Abstract : Although it is well-established that severe poisoning by organophosphorus (OP) compounds strongly affects the cardiorespiratory system, the effects of sub-lethal exposure to these compounds on the neural control of cardiovascular function are poorly explored. The aim of this study was to evaluate the effects of acute sub-lethal exposure to chlorpyrifos (CPF), a commonly used OP insecticide, on three basic reflex mechanisms involved in blood pressure regulation, the peripheral chemoreflex, the baroreflex and the Bezold-Jarisch reflex. Adult male Wistar rats were injected intraperitoneally with a single dose of CPF (30mg/kg) or saline (0.9%). 24h after injections, cardiovascular reflexes were tested in awake rats. Potassium cyanide (KCN) and phenylbiguanide (PBG) were injected intravenously to activate the chemoreflex and the Bezold-Jarisch reflex, respectively. The baroreflex was activated by phenylephrine and sodium nitroprusside infusions. Blood samples were taken for measurements of butyrylcholinesterase (BChE) activity while acetylcholinesterase (AChE) activity was measured in brainstem samples. Animals treated with CPF presented signs of intoxication such as ataxia, tremor, lacrimation, salivation, tetany, urination and defecation. The hypertensive and the bradycardic responses of the chemoreflex as well as the hypotensive and bradycardic responses of the Bezold-Jarisch reflex were attenuated in CPF treated animals (P<0.05). Concerning the baroreflex responses, CPF treatment reduced the bradycardia plateau, the range and the gain of the reflex (P<0.05). Plasma BChE and brainstem AChE were both reduced significantly after CPF treatment (P<0.05). Our results showed that acute sub-lethal exposure to CPF impairs the cardiovascular responses of homeostatic and defensive cardiovascular reflexes. These effects are associated with a marked inhibition of plasma BChE and brainstem AChE.
ESTHER : Cunha_2018_Toxicology_398-399_13
PubMedSearch : Cunha_2018_Toxicology_398-399_13
PubMedID: 29471072

Title : Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production - Argueso_2009_Genome.Res_19_2258
Author(s) : Argueso JL , Carazzolle MF , Mieczkowski PA , Duarte FM , Netto OV , Missawa SK , Galzerani F , Costa GG , Vidal RO , Noronha MF , Dominska M , Andrietta MG , Andrietta SR , Cunha AF , Gomes LH , Tavares FC , Alcarde AR , Dietrich FS , McCusker JH , Petes TD , Pereira GA
Ref : Genome Res , 19 :2258 , 2009
Abstract : Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (approximately 2 SNPs/kb) and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, with breakpoints within repetitive DNA sequences. Despite its complex karyotype, this diploid, when sporulated, had a high frequency of viable spores. Hybrid diploids formed by outcrossing with the laboratory strain S288c also displayed good spore viability. Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures. We also explored features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high ethanol and cell mass production and high temperature and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation of a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies.
ESTHER : Argueso_2009_Genome.Res_19_2258
PubMedSearch : Argueso_2009_Genome.Res_19_2258
PubMedID: 19812109
Gene_locus related to this paper: yeast-AIM2 , yeast-BST1 , yeast-cbpy1 , yeast-cld1 , yeast-dap1 , yeast-dap2 , yeast-dlhh , yeast-ECM18 , yeast-FSH1 , yeast-FSH3 , yeast-ict1 , yeast-kex01 , yeast-LDH1 , yeast-MCFS1 , yeast-MCFS2 , yeast-met2 , yeast-mgll , yeast-pdat , yeast-ppme1 , yeast-ROG1 , yeast-SAY1 , yeast-tgl1 , yeast-tgl2 , yeast-yby9 , yeast-YDL109C , yeast-YDR428C , yeast-YDR444W , yeast-yg19 , yeast-yj77 , yeast-yjg8 , yeast-YLL012W , yeast-YLR020C , yeast-YLR118c , yeast-ym60 , yeast-ynl5 , yeast-yo059 , yeast-YPR147C , yeast-hda1

Title : A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao - Mondego_2008_BMC.Genomics_9_548
Author(s) : Mondego JM , Carazzolle MF , Costa GG , Formighieri EF , Parizzi LP , Rincones J , Cotomacci C , Carraro DM , Cunha AF , Carrer H , Vidal RO , Estrela RC , Garcia O , Thomazella DP , de Oliveira BV , Pires AB , Rio MC , Araujo MR , de Moraes MH , Castro LA , Gramacho KP , Goncalves MS , Neto JP , Neto AG , Barbosa LV , Guiltinan MJ , Bailey BA , Meinhardt LW , Cascardo JC , Pereira GA
Ref : BMC Genomics , 9 :548 , 2008
Abstract : BACKGROUND: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9x coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. RESULTS: Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. CONCLUSION: This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/cacao pathosystem.
ESTHER : Mondego_2008_BMC.Genomics_9_548
PubMedSearch : Mondego_2008_BMC.Genomics_9_548
PubMedID: 19019209
Gene_locus related to this paper: monpe-e2l806 , monpe-e2lyh7 , monpe-e2lz46 , monro-v2x3v0 , monro-v2yx67 , monpe-e2lnh8