UPF0255

Relationship

Family: UPF0255

Block: X

Parent Family: No family

Comment

DUF1100 UPF0255. The family was split in two. Duf_1100-S with active site (S217 in (GXSXG) D300 H329) as in artni-Q93NG6, and Duf_1100-R (R53, D203, R 272 (in GXRXG)) as in FrsA. This family is represented by a C-C bond cleaving alpha/beta-hydrolase involved in nicotine degradation: 2,6-dihydroxy-pseudo-oxynicotine hydrolase from the nicotine-degradation pathway of Arthrobacter nicotinovorans (artni-Q93NG6) (Schleberger et al.). The fermentation/respiration switch protein FrsA (product of the yafA gene in E. Coli (ecoli-yafa)(vibvy-y856) binds the dephosphorylated form of glucose-specific enzyme IIAGl the interaction increases the glucose fermentation under oxygen-limited conditions. FrsA is a pyruvate decarboxylase (Lee et al.)(In Scop 2,6-dihydropseudooxynicotine hydrolase) Thus there are two functions and two different active sites in this family (nucleophile elbow: GXSXG in hydrolase, GXRXG in decarboxylase). However (Kellett et al.) showed Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase. The family also includes the hydrolytic polyketide shortening protein Ayg1 from fungi (Fujii et al.), and Fus2 from Gibberella species, which is part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Fus2 catalyses closure of the 2-pyrrolidone ring of the intermediate 20-hydroxy-prefusarin to form another intermediate, 20-hydroxy-fusarin, which is then oxidized by Fus8 (Niehaus et al.). Other members of this family are still uncharacterised

Database

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

No structure scheme yet for this family

No Genes Proteins

No fragment of genes

No fragment of genes

Substrates in UPF0255 family (1)

No Inhibitor

References (6)

Title : Genetic manipulation of the Fusarium fujikuroi fusarin gene cluster yields insight into the complex regulation and fusarin biosynthetic pathway - Niehaus_2013_Chem.Biol_20_1055
Author(s) : Niehaus EM , Kleigrewe K , Wiemann P , Studt L , Sieber CM , Connolly LR , Freitag M , Guldener U , Tudzynski B , Humpf HU
Ref : Chemical Biology , 20 :1055 , 2013
PubMedID: 23932525
Gene_locus related to this paper: gibf5-fus2 , gibf5-fus5

Title : Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase - Kellett_2013_Biochemistry_52_1842
Author(s) : Kellett WF , Brunk E , Desai BJ , Fedorov AA , Almo SC , Gerlt JA , Rothlisberger U , Richards NG
Ref : Biochemistry , 52 :1842 , 2013
PubMedID: 23452154
Gene_locus related to this paper: vibvy-y856

Title : FrsA functions as a cofactor-independent decarboxylase to control metabolic flux - Lee_2011_Nat.Chem.Biol_7_434
Author(s) : Lee KJ , Jeong CS , An YJ , Lee HJ , Park SJ , Seok YJ , Kim P , Lee JH , Lee KH , Cha SS
Ref : Nat Chemical Biology , 7 :434 , 2011
PubMedID: 21623357
Gene_locus related to this paper: vibvy-y856

Title : Structure and action of a C-C bond cleaving alpha\/beta-hydrolase involved in nicotine degradation - Schleberger_2007_J.Mol.Biol_367_409
Author(s) : Schleberger C , Sachelaru P , Brandsch R , Schulz GE
Ref : Journal of Molecular Biology , 367 :409 , 2007
PubMedID: 17275835
Gene_locus related to this paper: artni-Q93NG6

Title : A novel fermentation\/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli - Koo_2004_J.Biol.Chem_279_31613
Author(s) : Koo BM , Yoon MJ , Lee CR , Nam TW , Choe YJ , Jaffe H , Peterkofsky A , Seok YJ
Ref : Journal of Biological Chemistry , 279 :31613 , 2004
PubMedID: 15169777
Gene_locus related to this paper: vibvy-y856

Title : Hydrolytic polyketide shortening by ayg1p, a novel enzyme involved in fungal melanin biosynthesis - Fujii_2004_J.Biol.Chem_279_44613
Author(s) : Fujii I , Yasuoka Y , Tsai HF , Chang YC , Kwon-Chung KJ , Ebizuka Y
Ref : Journal of Biological Chemistry , 279 :44613 , 2004
PubMedID: 15310761
Gene_locus related to this paper: aspfu-AYG1