Koo_2004_J.Biol.Chem_279_31613

Reference

Title : A novel fermentation\/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli - Koo_2004_J.Biol.Chem_279_31613
Author(s) : Koo BM , Yoon MJ , Lee CR , Nam TW , Choe YJ , Jaffe H , Peterkofsky A , Seok YJ
Ref : Journal of Biological Chemistry , 279 :31613 , 2004
Abstract :

The bacterial phosphoenolpyruvate:sugar phosphotransferase system regulates a variety of physiological processes as well as effecting sugar transport. The crr gene product (enzyme IIA(Glc) (IIA(Glc))) mediates some of these regulatory phenomena. In this report, we characterize a novel IIA(Glc)-binding protein from Escherichia coli extracts, discovered using ligand-fishing with surface plasmon resonance spectroscopy. This protein, which we named FrsA (fermentation/respiration switch protein), is the 47-kDa product of the yafA gene, previously denoted as "function unknown." FrsA forms a 1:1 complex specifically with the unphosphorylated form of IIA(Glc), with the highest affinity of any protein thus far shown to interact with IIA(Glc). Orthologs of FrsA have been found to exist only in facultative anaerobes belonging to the gamma-proteobacterial group. Disruption of frsA increased cellular respiration on several sugars including glucose, while increased FrsA expression resulted in an increased fermentation rate on these sugars with the concomitant accumulation of mixed-acid fermentation products. These results suggest that IIA(Glc) regulates the flux between respiration and fermentation pathways by sensing the available sugar species via a phosphorylation state-dependent interaction with FrsA.

PubMedSearch : Koo_2004_J.Biol.Chem_279_31613
PubMedID: 15169777
Gene_locus related to this paper: vibvy-y856

Related information

Gene_locus vibvy-y856
Family UPF0255    Duf_1100-S    Duf_1100-R

Citations formats

Koo BM, Yoon MJ, Lee CR, Nam TW, Choe YJ, Jaffe H, Peterkofsky A, Seok YJ (2004)
A novel fermentation\/respiration switch protein regulated by enzyme IIAGlc in Escherichia coli
Journal of Biological Chemistry 279 :31613

Koo BM, Yoon MJ, Lee CR, Nam TW, Choe YJ, Jaffe H, Peterkofsky A, Seok YJ (2004)
Journal of Biological Chemistry 279 :31613