9rosa-CXE1

Malus pumila (Paradise apple); Malus domestica (Apple) (Pyrus malus) CXE1 carboxylesterase MdCXE1 (EC 3.1.1.1)

Comment

Other strains: Malus pumila (Paradise apple)\; Malus domestica (Apple) (Pyrus malus) || Marshall S.D.G., Oakeshott J.G., Russell R.J., Plummer K.M., Newcomb R.D || MdCXE1 is expressed at low levels during early stages of fruit development, rising to a peak of expression in apple fruit at harvest maturity. MdCXE1 hydrolyse 4-methyl umbelliferyl ester substrates that included those containing acyl moieties that are found in esters produced by apple fruit. MdCXE1 is inhibited by organophosphates. MdCXE1 showed increasing affinity (Km) but decreasing turnover (kcat) as substrate acyl carbon length increases from C2 to C16. MdCXE1 may have an impact on apple flavour through its ability to hydrolyse relevant flavour esters in ripe apple fruit.

Relationship

Family : Plant_carboxylesterase

Block : H

Position in NCBI Life Tree : Malus pumila

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Viridiplantae N E > Streptophyta N E > Streptophytina N E > Embryophyta N E > Tracheophyta N E > Euphyllophyta N E > Spermatophyta N E > Magnoliophyta N E > Mesangiospermae N E > eudicotyledons N E > Gunneridae N E > Pentapetalae N E > rosids N E > fabids N E > Rosales N E > Rosaceae N E > Maloideae N E > Maleae N E > Malus N E > Malus pumila N E

Molecular evidence

No mutation

No structure

No kinetic

No disease

No substrate

No inhibitor

Database

Sequence

Peptide

MTTSLDSSTQ KTAPSWTSLP WRTRVSLSVL SKITDAARRA DGTVNRRLMN ILDFKSSATP AAPVRGVTSS DVTVDPARKL WFRLFVPQST LSTPSDLPVI VFFHGGGFTF LSPASFAYNA VCRKFARKFP AVVVSVNYRL CPEHRYPSPY DDGFDVLTFL DQNDDVLPKN ADRSRIFLAG DSAGANVAHH VAVRAAREKD RMRVVKPVGL ISIQPFFGGE ERVESEIRLR GAPLVSVGRT DWLWKVFLPD GSNRDHEAAN VSGPNAVDIS GLEYPNTIVF TGGLDPLLDR QRRYYQWLKK SGKEAKLIEY PNMVHAFYVF PELPESNQLI NQVKDFIASV

References (2)

Title : Biochemical characterisation of MdCXE1, a carboxylesterase from apple that is expressed during fruit ripening - Souleyre_2011_Phytochemistry_72_564
Author(s) : Souleyre EJ , Marshall SD , Oakeshott JG , Russell RJ , Plummer KM , Newcomb RD
Ref : Phytochemistry , 72 :564 , 2011
PubMedID: 21315388
Gene_locus related to this paper: 9rosa-CXE1 , 9rosa-CXE2 , 9rosa-CXE3 , 9rosa-CXE4 , 9rosa-CXE5 , 9rosa-CXE6 , 9rosa-CXE7 , 9rosa-CXE8 , 9rosa-CXE9 , 9rosa-CXE10 , 9rosa-CXE11 , 9rosa-CXE12