(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Clostridia: NE > Clostridiales: NE > Clostridiaceae: NE > Clostridium: NE > environmental samples: NE > Clostridium hathewayi CAG:224: NE
Warning: This entry is a compilation of different species or line or strain with more than 90% amino acid identity. You can retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Clostridium hathewayi DSM 13479: N, E.
[Clostridium] hathewayi DSM 13479: N, E.
Clostridium hathewayi WAL-18680: N, E.
Clostridium hathewayi 12489931: N, E.
Hungatella hathewayi: N, E.
[Clostridium] hathewayi WAL-18680: N, E.
[Clostridium] hathewayi 12489931: N, E.
Molecular evidence
Database
No mutation 1 structure: 5A2G: An esterase from anaerobic Clostridium hathewayi Clath_Est1 can hydrolyze aliphatic aromatic polyesters No kinetic
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MAKQFLYDSLPVVETKAGKLRGYQWEGTYIFKGIRYARANRFQLPEEVEP WEGVKEAASYGFVCPMLTRDHPQGELLVPHRYWPQDEDCLSLNIWSQSLD RSAKKPVMFWIHGGAFSMGSSIEQKAYNGENMSRYGDVVVVTVNHRLNIL GYLDLSPYGERYAGSANAGQADLVAALKWVRDNIEAFGGDPDNVTIFGQS GGGMKVSGLMQTPEADGLFHRAMIMSGVAGDVLPYSTGDSRPLIQAMLKE LGLAEQEAGRLETVPYYDLAAAYNRVSPAIARAGGYIGCTPRPDDFYKGE GPAVGFTDHAKTIPVMVGTVFGEFAMMPLPFNKETISEAELDEILDKRFQ GHGKELKAVFAEAYPGKSPVDLLTLDTIFRGPTKEFVRSLAAAGGSVYSY LFALEFPYQNQKTAWHCSDIPFIFHNTELVPVANIPEVSDRLEEQMFGAV MAFARSGKPEYGGLPQWPASRENDEATMIFDRVCEVRHNHDNRLLKLHAE LSPKFDLAAVMAEMGDEIQH
Recently, a variety of biodegradable polymers have been developed as alternatives to recalcitrant materials. Although many studies on polyester biodegradability have focused on aerobic environments, there is much less known on biodegradation of polyesters in natural and artificial anaerobic habitats. Consequently, the potential of anaerobic biogas sludge to hydrolyze the synthetic compostable polyester PBAT (poly(butylene adipate-co-butylene terephthalate) was evaluated in this study. On the basis of reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, accumulation of terephthalic acid (Ta) was observed in all anaerobic batches within the first 14 days. Thereafter, a decline of Ta was observed, which occurred presumably due to consumption by the microbial population. The esterase Chath_Est1 from the anaerobic risk 1 strain Clostridium hathewayi DSM-13479 was found to hydrolyze PBAT. Detailed characterization of this esterase including elucidation of the crystal structure was performed. The crystal structure indicates that Chath_Est1 belongs to the alpha/beta-hydrolases family. This study gives a clear hint that also micro-organisms in anaerobic habitats can degrade manmade PBAT.
Clostridium hathewayi; Hungatella hathewayi esterase of aliphatic aromatic polyesters (Clath_Est1)
