Gene_locus Report for: asptn-q0ci40 Aspergillus terreus (strain NIH 2624 / FGSC A1156). ATEG_06644 AtFaeD
Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Ascomycota: N E > saccharomyceta: N E > Pezizomycotina: N E > leotiomyceta: N E > Eurotiomycetes: N E > Eurotiomycetidae: N E > Eurotiales: N E > Aspergillaceae: N E > Aspergillus: N E > Aspergillus terreus: N E 6_AlphaBeta_hydrolase :
asptn-q0c7g6 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0c9n3 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0c860 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cat4 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cgt3 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0ci39 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0cis0 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cj96 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-q0cms8 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0cq04 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0crf6 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0ct87 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0ctb4 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cz24 Aspergillus terreus (strain NIH 2624) predicted protein .
A85-EsteraseD-FGH :
asptn-q0cip6 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Esterase D .
A85-IroE-IroD-Fes-Yiel :
asptn-q0cqg2 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
ABHD13-BEM46 :
asptn-q0cpn0 Aspergillus terreus (strain NIH 2624 / FGSC A1156). Hydrolase_4 domain-containing protein .
abh_upf0017 :
asptn-q0ch57 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Acetylxylan_esterase :
asptn-q0cqh8 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Acetylxylan esterase ,
asptn-q0cnm5 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Acetylxylan esterase .
Acidic_Lipase :
asptn-q0cet1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cib3 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Bacterial_esterase :
asptn-q0cy59 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0czw6 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein .
BD-FAE :
asptn-q0cvd2 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-trt4 Aspergillus terreus (strain NIH 2624 / FGSC A1156). Terretonin synthesis protein 4 ,
asptn-5moas Aspergillus terreus (strain NIH 2624 / FGSC A1156). Non-reducing polyketide synthase ATEG_03629. Thioesterase last domain .
Canar_LipB :
asptn-q0cjs0 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cpv1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
Carboxypeptidase_S10 :
asptn-q0cg19 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Carboxypeptidase S1 ,
asptn-kex1 Aspergillus terreus (strain NIH 2624 / FGSC A1156). Carboxypeptidase D ,
asptn-cbpya Aspergillus terreus (strain NIH 2624 / FGSC A1156). Carboxypeptidase Y homolog A .
CFTR-inhibitory-factor_Cif :
asptn-q0ckr1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0cp98 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
CGI-58_ABHD5_ABHD4 :
asptn-q0cr64 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Cutinase :
asptn-cuti1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Cutin hydrolase 1 ,
asptn-cuti2 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Cutin hydrolase 2 ,
asptn-cuti3 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Cutin hydrolase 3 ,
asptn-cuti4 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Cutin hydrolase 4 ,
asptn-cuti5 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Cutin hydrolase 5 ,
asptn-q0cz47 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
DPP4N_Peptidase_S9 :
asptn-dapb Aspergillus terreus (strain NIH 2624 / FGSC A1156) Probable dipeptidyl-aminopeptidase B ,
asptn-q0cm15 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Duf_726 :
asptn-q0cr50 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cyj0 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Duf_829 :
asptn-q0cn88 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0d199 Aspergillus terreus (strain NIH 2624) Predicted protein .
Epoxide_hydrolase :
asptn-q0cb76 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0ccj0 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0ci99 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Esterase_phb :
asptn-axe1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Probable acetylxylan esterase A .
FaeC :
asptn-faec Aspergillus terreus (strain NIH 2624 / FGSC A1156) Ferulic acid esterase C ,
asptn-q0cwm0 Aspergillus terreus (strain NIH 2624 / FGSC A1156). CBM1 domain-containing protein .
FSH1 :
asptn-LOVG Aspergillus terreus (strain NIH 2624 / FGSC A1156) Esterase LovG ,
aspte-Q9Y7C9 Aspergillus terreus hypothetical protein ,
asptn-azpb5 Aspergillus terreus (strain NIH 2624 / FGSC A1156). Azasperpyranone A biosynthesis cluster B protein ATEG_07663 .
Fungal-Bact_LIP :
asptn-q0cj90 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cmu9 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0cry4 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein ,
asptn-q0cz14 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0czh3 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
Fungal_carboxylesterase_lipase :
asptn-q0c7i9 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0c7j4 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0c8p0 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0c9e7 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0ccb4 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cce4 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cjm6 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0ckc5 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-q0cmr8 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cqj2 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0csf6 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-q0cug8 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0cum8 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-q0cv09 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0cwz7 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0cxl7 Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein ,
asptn-q0cya0 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0czb3 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein ,
asptn-q0d143 Aspergillus terreus (strain NIH 2624) putative uncharacterized protein .
Fusarinine_C_esterase_sidJ :
asptn-q0cp11 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Dolichol-phosphate mannosyltransferase ,
asptn-q0cuc2 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Glucuronoyl_esterase :
asptn-q0czd9 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Uncharacterized protein .
GTSAGmotif :
asptn-q0cg08 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
Haloperoxidase :
asptn-q0d142 Aspergillus terreus (strain NIH 2624) non-heme chloroperoxidase .
Hormone-sensitive_lipase_like :
asptn-q0cgs6 Aspergillus terreus (strain NIH 2624) predicted protein ,
asptn-pytb Aspergillus terreus (strain NIH 2624 / FGSC A1156). Pyranterreones biosynthesis cluster protein B; .
Kynurenine-formamidase :
asptn-q0d1j3 Aspergillus terreus (strain NIH 2624 / FGSC A1156). N-formylkynurenine formamidase .
LIDHydrolase :
asptn-q0cd92 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein .
Lipase_3 :
asptn-atg15 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Autophagy-related protein 15 ,
asptn-faea Aspergillus terreus (strain NIH 2624/FGSC A1156) Ferulic acid esterase A .
MpaH :
asptn-q0ct68 Aspergillus terreus (strain NIH 2624 / FGSC A1156). AB hydrolase-1 domain-containing protein .
PGAP1 :
asptn-bst1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) GPI inositol-deacylase .
PPase_methylesterase_euk :
asptn-q0cwi5 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Protein phosphatase methylesterase 1 .
Prolylcarboxypeptidase :
asptn-q0c8h2 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Putative uncharacterized protein ,
asptn-q0cvt7 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Predicted protein .
Prolyl_oligopeptidase_S9 :
asptn-dpp5 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Dipeptidyl-peptidase V .
Tannase :
asptn-faeb1 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Ferulic acid esterase B-1 ,
asptn-faeb2 Aspergillus terreus (strain NIH 2624 / FGSC A1156) Ferulic acid esterase B-2 .
Thioesterase :
aspte-AT1 Aspergillus terreus polyketide synthase. Terrein biosynthesis cluster protein terA, from aa 1680 thioesterase module ,
aspte-AT4 Aspergillus terreus polyketide synthase (fragment thioesterase module) ,
asptn-apva Aspergillus terreus (strain NIH 2624 / FGSC A1156). Aspulvinone E synthase Thioesterase domain ,
asptn-atqa Aspergillus terreus (strain NIH 2624 / FGSC A1156). Didemethylasterriquinone synthetase Thioesterase domain ,
asptn-btya Aspergillus terreus (strain NIH 2624 / FGSC A1156). Butyrolactone IIa synthetase Thioesterase domain ,
aspte-curs2 Aspergillus terreus. Dehydrocurvularin biosynthesis protein 2. Thioesterase domain ,
asptn-atra Aspergillus terreus (strain NIH 2624 / FGSC A1156). Atromentin synthetase. Thioesterase domain ,
aspte-mela Aspergillus terreus. Nonribosomal peptide synthetase melA. Thioesterase domain ,
asptn-mela Aspergillus terreus (strain NIH 2624 / FGSC A1156). Nonribosomal peptide synthase melA. Thioesterase domain ,
asptn-pnga Aspergillus terreus (strain NIH 2624 / FGSC A1156). Phenguignardic acid synthase. Thioesterase domain ,
aspte-pyti Aspergillus terreus. Pyranterreones biosynthesis cluster protein I .
Thiohydrolase :
asptn-q0cnl5 Aspergillus terreus (strain NIH 2624 / FGSC A1156). Hydrolase_4 domain-containing protein Molecular evidence
Database
Sequence
Graphical view for this peptide sequence: asptn-q0ci40 Colored MSA for FaeC (raw)
MRYSAATLAASAAGLAANVAANSAGCGTTPSLSSGLNSLTVNGQNREYII
QTPDNYDSSTPHKLVIGYHWRDGTMNNVVDGGFYGLSYLAGDVPTIFVAP
QGIDNGWANSNGDDIAFTDAILEAVESSYCIDEERRFATGWSWGGGMSYS
VACSRADVFRAVAVLSGAEISGCDGGSVPIAYFGQHGVSDSVLPIDLGRQ
LRDHFVQVNGCDAASPQEPAAGSGTHTVTDYTGCSDGHPVQWVAFDGDHE
PTPSDAGAQNSFTGGLIWDFFSQF
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M R Y S A A T L A A S A A G L A A N V A A N S A G C G T T P S L S S G L N S L T V N G Q N R E Y I I Q T P D N Y D S S T P H K L V I G Y H W R D G T M N N V V D G G F Y G L S Y L A G D V P T I F V A P Q G I D N G W A N S N G D D I A F T D A I L E A V E S S Y C I D E E R R F A T G W S W G G G M S Y S V A C S R A D V F R A V A V L S G A E I S G C D G G S V P I A Y F G Q H G V S D S V L P I D L G R Q L R D H F V Q V N G C D A A S P Q E P A A G S G T H T V T D Y T G C S D G H P V Q W V A F D G D H E P T P S D A G A Q N S F T G G L I W D F F S Q F
Reference
Title: Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate-active enzymes (CAZy) databaseMakela MR , Dilokpimol A , Koskela SM , Kuuskeri J , de Vries RP , Hilden K Ref: Microb Biotechnol, 11 :869, 2018 : PubMed Abstract ESTHER: Makela_2018_Microb.Biotechnol_11_869 PubMedSearch: Makela 2018 Microb.Biotechnol 11 869 PubMedID: 29697197 Gene_locus related to this paper: asptn-q0ci40 Abstract
Feruloyl esterases (FAEs) are accessory enzymes for plant biomass degradation, which catalyse hydrolysis of carboxylic ester linkages between hydroxycinnamic acids and plant cell-wall carbohydrates. They are a diverse group of enzymes evolved from, e.g. acetyl xylan esterases (AXEs), lipases and tannases, thus complicating their classification and prediction of function by sequence similarity. Recently, an increasing number of fungal FAEs have been biochemically characterized, owing to their potential in various biotechnological applications and multitude of candidate FAEs in fungal genomes. However, only part of the fungal FAEs are included in Carbohydrate Esterase family 1 (CE1) of the carbohydrate-active enzymes (CAZy) database. In this work, we performed a phylogenetic analysis that divided the fungal members of CE1 into five subfamilies of which three contained characterized enzymes with conserved activities. Conservation within one of the subfamilies was confirmed by characterization of an additional CE1 enzyme from Aspergillus terreus. Recombinant A. terreus FaeD (AtFaeD) showed broad specificity towards synthetic methyl and ethyl esters, and released ferulic acid from plant biomass substrates, demonstrating its true FAE activity and interesting features as potential biocatalyst. The subfamily division of the fungal CE1 members enables more efficient selection of candidate enzymes for biotechnological processes.
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