(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Firmicutes: NE > Clostridia: NE > Clostridiales: NE > Clostridiaceae: NE > Clostridium: NE > Clostridium ljungdahlii: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA IKYDNDGKVYDFTSEAAPVESNYKLPTYVNIDNFTEKNVAIGDEKYKLPG VLSIPKGKGPFPAVVLVHGSGANDMDETYGDTKVFKDISAGLASKGIAVL RYNKRSNEYPSLTAIDTKLDLNKETVNDAVDAVKLLKKTKEIDSSKVFVL GHSQGAMLTPTILKDCGNSGAAGGIMMAGPVDFLDTLLEQSKYLNSIGYM DEDNLKYIQNLYDLVKNNKDFPKNISENTIIFHAYPYYWLSVKNSTQAQD AVDIKNPLLILQGKRDYQVAASNLEKWKNILKDKQNVEYNSYDKLNHFFI EGEGKMTPEEYGKGNVPKYVIDDIVSWISKYSK
Clostridium ljungdahlii is an anaerobic homoacetogen, able to ferment sugars, other organic compounds, or CO(2)/H(2) and synthesis gas (CO/H(2)). The latter feature makes it an interesting microbe for the biotech industry, as important bulk chemicals and proteins can be produced at the expense of CO(2), thus combining industrial needs with sustained reduction of CO and CO(2) in the atmosphere. Sequencing the complete genome of C. ljungdahlii revealed that it comprises 4,630,065 bp and is one of the largest clostridial genomes known to date. Experimental data and in silico comparisons revealed a third mode of anaerobic homoacetogenic metabolism. Unlike other organisms such as Moorella thermoacetica or Acetobacterium woodii, neither cytochromes nor sodium ions are involved in energy generation. Instead, an Rnf system is present, by which proton translocation can be performed. An electroporation procedure has been developed to transform the organism with plasmids bearing heterologous genes for butanol production. Successful expression of these genes could be demonstrated, leading to formation of the biofuel. Thus, C. ljungdahlii can be used as a unique microbial production platform based on synthesis gas and carbon dioxide/hydrogen mixtures.
