Gene_locus Report for: malgo-a8qcw4

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Eukaryota: N E > Opisthokonta: N E > Fungi: N E > Dikarya: N E > Basidiomycota: N E > Ustilaginomycotina: N E > Malasseziomycetes: N E > Malasseziales: N E > Malasseziaceae: N E > Malassezia: N E > Malassezia globosa: N E

A85-EsteraseD-FGH : malgo-a8q902Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
abh_upf0017 : malgo-a8pwm2Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Acidic_Lipase : malgo-a8q4e6Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
BD-FAE : malgo-a8pzh3Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
CGI-58_ABHD5_ABHD4 : malgo-a8q1i5Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Duf_726 : malgo-a8pwh6Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Fungal-Bact_LIP : malgo-a8px35Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein, malgo-a8q9m3Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein, malgo-a8qcv4Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Hypothetical secretory lipase (Family LIP), malgo-a8qcv7Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Hypothetical secretory lipase (Family LIP), malgo-a8qdf0Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Fungal_carboxylesterase_lipase : malgo-a8qcr1Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Fusarinine_C_esterase_sidJ : malgo-a8pwr6Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
LIDHydrolase : malgo-a8pvh8Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
Lipase_3 : malgo-a8puy5 Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) MGL_0799), MgMDL2, secretory lipase (Family 3), malgo-a8puy1 Malassezia globosa (strain ATCC MYA-4612 / CBS 7966) SMG1, LIP1, secretory lipase (Family 3).
Monoglyceridelipase_lysophospholip : malgo-a8q8v5Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein, malgo-a8q9n0Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Putative uncharacterized protein.
PPase_methylesterase_euk : malgo-a8q7d0Malassezia globosa (strain ATCC 96807 / CBS 7966) (Dandruff-associated fungus) Protein phosphatase methylesterase 1

Title: Purification and characterization of a secretory lipolytic enzyme, MgLIP2, from Malassezia globosa
Juntachai W, Oura T, Kajiwara S
Ref: Microbiology (Reading), 157:3492, 2011 : PubMed
Abstract


ESTHER: Juntachai_2011_Microbiol.(Reading)_157_3492
PubMedSearch: Juntachai 2011 Microbiol.(Reading) 157 3492
PubMedID: 22016565
Gene_locus related to this paper: malgo-a8qcw4

Abstract

Malassezia globosa is a lipid-dependent yeast that is found on the human skin and is associated with various skin disorders, including dandruff and seborrhoeic dermatitis (SD). Despite its important role in skin diseases, the molecular basis for its pathogenicity is poorly understood. The current hypothesis is that dandruff and SD are linked to fatty acid metabolism and secretory lipolytic enzymes, which hydrolyse sebaceous lipids and release irritating free fatty acids. A previous genomic analysis of M. globosa identified a family of 13 homologous genes predicted to encode secreted lipases. We have also reported that M. globosa had significantly higher extracellular lipase activity compared with other species. To identify the major secretory lipases of this yeast during its growth, we successfully purified and characterized an extracellular lipase MgLIP2. Based on MALDI-TOF MS, the peptide mass fingerprint of a tryptically digested protein MgLIP2 corresponded to ORF MGL_4054 of M. globosa. This lipase showed high esterase activity against 4-nitrophenyl palmitate and 1-naphthyl palmitate but not 1-naphthyl acetate. This enzyme had optimal activity at 30 degreesC and pH 5.0. Furthermore, the activity significantly increased in the presence of Triton X-100 and was partially inhibited by PMSF but was unaffected by univalent and divalent metal ions.



        

Title: Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens
Xu J, Saunders CW, Hu P, Grant RA, Boekhout T, Kuramae EE, Kronstad JW, Deangelis YM, Reeder NL and Dawson TL, Jr. <11 more author(s)> Xu J, Saunders CW, Hu P, Grant RA, Boekhout T, Kuramae EE, Kronstad JW, Deangelis YM, Reeder NL, Johnstone KR, Leland M, Fieno AM, Begley WM, Sun Y, Lacey MP, Chaudhary T, Keough T, Chu L, Sears R, Yuan B, Dawson TL, Jr. (- 11)
Ref: Proc Natl Acad Sci U S A, 104:18730, 2007 : PubMed
Abstract


ESTHER: Xu_2007_Proc.Natl.Acad.Sci.U.S.A_104_18730
PubMedSearch: Xu 2007 Proc.Natl.Acad.Sci.U.S.A 104 18730
PubMedID: 18000048
Gene_locus related to this paper: malgo-a8puy5, malgo-a8puy1, malgo-a8pvh8, malgo-a8pwh6, malgo-a8pwm2, malgo-a8pwr6, malgo-a8px35, malgo-a8pzh3, malgo-a8q1i5, malgo-a8q4e6, malgo-a8q7d0, malgo-a8q8v5, malgo-a8q9m3, malgo-a8q9n0, malgo-a8q902, malgo-a8qcr1, malgo-a8qcv4, malgo-a8qcv7, malgo-a8qcw4, malgo-a8qdf0

Abstract

Fungi in the genus Malassezia are ubiquitous skin residents of humans and other warm-blooded animals. Malassezia are involved in disorders including dandruff and seborrheic dermatitis, which together affect >50% of humans. Despite the importance of Malassezia in common skin diseases, remarkably little is known at the molecular level. We describe the genome, secretory proteome, and expression of selected genes of Malassezia globosa. Further, we report a comparative survey of the genome and secretory proteome of Malassezia restricta, a close relative implicated in similar skin disorders. Adaptation to the skin environment and associated pathogenicity may be due to unique metabolic limitations and capabilities. For example, the lipid dependence of M. globosa can be explained by the apparent absence of a fatty acid synthase gene. The inability to synthesize fatty acids may be complemented by the presence of multiple secreted lipases to aid in harvesting host lipids. In addition, an abundance of genes encoding secreted hydrolases (e.g., lipases, phospholipases, aspartyl proteases, and acid sphingomyelinases) was found in the M. globosa genome. In contrast, the phylogenetically closely related plant pathogen Ustilago maydis encodes a different arsenal of extracellular hydrolases with more copies of glycosyl hydrolase genes. M. globosa shares a similar arsenal of extracellular hydrolases with the phylogenetically distant human pathogen, Candida albicans, which occupies a similar niche, indicating the importance of host-specific adaptation. The M. globosa genome sequence also revealed the presence of mating-type genes, providing an indication that Malassezia may be capable of sex.