D74G_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Peripheral Anionic Site Peripheral Anionic Site\/signal transduction\/allosteric modulation\/substrate inhibition. Affects conformation of active site and PAS Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165 || Signal transduction Affects conformation of active site and PAS Shafferman_1992_EMBO.J_11_3561 Shafferman_1992_4th.ChE.Meeting.Eilat__165

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Peripheral Anionic Site || Substrate inhibition || Signal transduction || Allosteric modulation

Torpedo_number : 72

Kinetic Parameter : BW284C51_D74G_human-ACHE, Decamethonium_D74G_human-ACHE, Propidium_D74G_human-ACHE, Edrophonium_D74G_human-ACHE, Acetylthiocholine_D74G_human-ACHE

News : No news

Comment :
p.D74G Asp74Gly (p.D105Gly Asp105Gly in primary sequence with 31 amino-acids signal peptide) Peripheral Anionic Site\/signal transduction\/allosteric modulation. Affects conformation of active site and PAS\;

References (4)

Title : Acetylthiocholine binds to asp74 at the peripheral site of human acetylcholinesterase as the first step in the catalytic pathway - Mallender_2000_Biochemistry_39_7753
Author(s) : Mallender WD , Szegletes T , Rosenberry TL
Ref : Biochemistry , 39 :7753 , 2000
PubMedID: 10869180

Title : Acetylcholinesterase Catalysis - Protein Engineering Studies -
Author(s) : Shafferman A , Velan B
Ref : In Multidisciplinary approaches to cholinesterase functions - Proceedings of Fourth International Meeting on Cholinesterases , (Shafferman, A. and Velan, B., Eds) Plenum Press, New York :165 , 1992
PubMedID:

Title : Mutagenesis of human acetylcholinesterase. Identification of residues involved in catalytic activity and in polypeptide folding - Shafferman_1992_J.Biol.Chem_267_17640
Author(s) : Shafferman A , Kronman C , Flashner Y , Leitner M , Grosfeld H , Ordentlich A , Gozes Y , Cohen S , Ariel N , Barak D , Harel M , Silman I , Sussman JL , Velan B
Ref : Journal of Biological Chemistry , 267 :17640 , 1992
PubMedID: 1517212

Title : Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center - Shafferman_1992_EMBO.J_11_3561
Author(s) : Shafferman A , Velan B , Ordentlich A , Kronman C , Grosfeld H , Leitner M , Flashner Y , Cohen S , Barak D , Ariel N
Ref : EMBO Journal , 11 :3561 , 1992
PubMedID: 1396557